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Virus-like particle vaccines expressing Toxoplasma gondii rhoptry protein 18 and microneme protein 8 provide enhanced protection

Toxoplasma gondii rhoptry protein 18 (ROP18) and microneme protein 8 (MIC8) are important invasion factors that mediate parasite multiplication. The main objective of this study was to determine the immunogenicity and efficacy by virus-like particle (VLP) vaccines of T. gondii ROP18 VLPs and MIC8 VL... Full description

Journal Title: Vaccine 2018-09-11, Vol.36 (38), p.5692-5700
Main Author: Lee, Su-Hwa
Other Authors: Kang, Hae-Ji , Lee, Dong-Hun , Kang, Sang-Moo , Quan, Fu-Shi
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Netherlands: Elsevier Ltd
ID: ISSN: 0264-410X
Link: https://www.ncbi.nlm.nih.gov/pubmed/30107996
Zum Text:
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title: Virus-like particle vaccines expressing Toxoplasma gondii rhoptry protein 18 and microneme protein 8 provide enhanced protection
format: Article
creator:
  • Lee, Su-Hwa
  • Kang, Hae-Ji
  • Lee, Dong-Hun
  • Kang, Sang-Moo
  • Quan, Fu-Shi
subjects:
  • Animals
  • Antibodies, Protozoan - blood
  • Antigens
  • Apoptosis - immunology
  • Cell Adhesion Molecules - immunology
  • Cell Line
  • Female
  • Health aspects
  • Immunoglobulin A - blood
  • Immunoglobulin G - blood
  • Interferon-gamma - blood
  • Interleukin-6 - blood
  • Medical colleges
  • Mice
  • Mice, Inbred BALB C
  • Microneme protein 8
  • Protein-Serine-Threonine Kinases - immunology
  • Protozoan Proteins - immunology
  • Protozoan Vaccines - immunology
  • Research institutes
  • Rhoptry protein 18
  • Sf9 Cells
  • Spodoptera
  • Synergistic effects
  • T cells
  • Toxoplasma gondii
  • Toxoplasmosis, Animal - immunology
  • Toxoplasmosis, Animal - prevention & control
  • Vaccination
  • Vaccine
  • Vaccines
  • Vaccines, Virus-Like Particle - immunology
  • Virus-like particles
ispartof: Vaccine, 2018-09-11, Vol.36 (38), p.5692-5700
description: Toxoplasma gondii rhoptry protein 18 (ROP18) and microneme protein 8 (MIC8) are important invasion factors that mediate parasite multiplication. The main objective of this study was to determine the immunogenicity and efficacy by virus-like particle (VLP) vaccines of T. gondii ROP18 VLPs and MIC8 VLPs in combination or individuals. Mice were intranasally immunized with single ROP18 VLPs or MIC8 VLPs, or a combination of ROP18 VLPs and MIC8 VLPs followed by challenge with T. gondii tachyzoites (GT1) through intraperitoneal injection or challenge with T. gondii (ME49) through oral infection. Vaccination with a combination of ROP18 VLPs and MIC8 VLPs resulted in more potent effects on inducing parasite neutralizing activity, memory T cell response, and reducing parasite burden compared to single VLP vaccination. Lower levels of inflammatory cytokines (IFN-γ, IL-6) and apoptosis responses were observed with VLP vaccine combination after challenge. These results suggest that VLP vaccines presenting both T. gondii ROP18 and MIC8 antigens confer enhanced protection compared to a single VLPs vaccine, and VLP platforms could be developed as potential vaccines against toxoplasmosis.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0264-410X
fulltext: fulltext
issn:
  • 0264-410X
  • 1873-2518
url: Link


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titleVirus-like particle vaccines expressing Toxoplasma gondii rhoptry protein 18 and microneme protein 8 provide enhanced protection
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descriptionToxoplasma gondii rhoptry protein 18 (ROP18) and microneme protein 8 (MIC8) are important invasion factors that mediate parasite multiplication. The main objective of this study was to determine the immunogenicity and efficacy by virus-like particle (VLP) vaccines of T. gondii ROP18 VLPs and MIC8 VLPs in combination or individuals. Mice were intranasally immunized with single ROP18 VLPs or MIC8 VLPs, or a combination of ROP18 VLPs and MIC8 VLPs followed by challenge with T. gondii tachyzoites (GT1) through intraperitoneal injection or challenge with T. gondii (ME49) through oral infection. Vaccination with a combination of ROP18 VLPs and MIC8 VLPs resulted in more potent effects on inducing parasite neutralizing activity, memory T cell response, and reducing parasite burden compared to single VLP vaccination. Lower levels of inflammatory cytokines (IFN-γ, IL-6) and apoptosis responses were observed with VLP vaccine combination after challenge. These results suggest that VLP vaccines presenting both T. gondii ROP18 and MIC8 antigens confer enhanced protection compared to a single VLPs vaccine, and VLP platforms could be developed as potential vaccines against toxoplasmosis.
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languageeng
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subjectAnimals ; Antibodies, Protozoan - blood ; Antigens ; Apoptosis - immunology ; Cell Adhesion Molecules - immunology ; Cell Line ; Female ; Health aspects ; Immunoglobulin A - blood ; Immunoglobulin G - blood ; Interferon-gamma - blood ; Interleukin-6 - blood ; Medical colleges ; Mice ; Mice, Inbred BALB C ; Microneme protein 8 ; Protein-Serine-Threonine Kinases - immunology ; Protozoan Proteins - immunology ; Protozoan Vaccines - immunology ; Research institutes ; Rhoptry protein 18 ; Sf9 Cells ; Spodoptera ; Synergistic effects ; T cells ; Toxoplasma gondii ; Toxoplasmosis, Animal - immunology ; Toxoplasmosis, Animal - prevention & control ; Vaccination ; Vaccine ; Vaccines ; Vaccines, Virus-Like Particle - immunology ; Virus-like particles
ispartofVaccine, 2018-09-11, Vol.36 (38), p.5692-5700
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descriptionToxoplasma gondii rhoptry protein 18 (ROP18) and microneme protein 8 (MIC8) are important invasion factors that mediate parasite multiplication. The main objective of this study was to determine the immunogenicity and efficacy by virus-like particle (VLP) vaccines of T. gondii ROP18 VLPs and MIC8 VLPs in combination or individuals. Mice were intranasally immunized with single ROP18 VLPs or MIC8 VLPs, or a combination of ROP18 VLPs and MIC8 VLPs followed by challenge with T. gondii tachyzoites (GT1) through intraperitoneal injection or challenge with T. gondii (ME49) through oral infection. Vaccination with a combination of ROP18 VLPs and MIC8 VLPs resulted in more potent effects on inducing parasite neutralizing activity, memory T cell response, and reducing parasite burden compared to single VLP vaccination. Lower levels of inflammatory cytokines (IFN-γ, IL-6) and apoptosis responses were observed with VLP vaccine combination after challenge. These results suggest that VLP vaccines presenting both T. gondii ROP18 and MIC8 antigens confer enhanced protection compared to a single VLPs vaccine, and VLP platforms could be developed as potential vaccines against toxoplasmosis.
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abstractToxoplasma gondii rhoptry protein 18 (ROP18) and microneme protein 8 (MIC8) are important invasion factors that mediate parasite multiplication. The main objective of this study was to determine the immunogenicity and efficacy by virus-like particle (VLP) vaccines of T. gondii ROP18 VLPs and MIC8 VLPs in combination or individuals. Mice were intranasally immunized with single ROP18 VLPs or MIC8 VLPs, or a combination of ROP18 VLPs and MIC8 VLPs followed by challenge with T. gondii tachyzoites (GT1) through intraperitoneal injection or challenge with T. gondii (ME49) through oral infection. Vaccination with a combination of ROP18 VLPs and MIC8 VLPs resulted in more potent effects on inducing parasite neutralizing activity, memory T cell response, and reducing parasite burden compared to single VLP vaccination. Lower levels of inflammatory cytokines (IFN-γ, IL-6) and apoptosis responses were observed with VLP vaccine combination after challenge. These results suggest that VLP vaccines presenting both T. gondii ROP18 and MIC8 antigens confer enhanced protection compared to a single VLPs vaccine, and VLP platforms could be developed as potential vaccines against toxoplasmosis.
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pmid30107996
doi10.1016/j.vaccine.2018.08.016