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Electrochemical sandwich immunoassay for Escherichia coli O157:H7 based on the use of magnetic nanoparticles and graphene functionalized with electrocatalytically active Au@Pt core/shell nanoparticles

A highly sensitive electrochemical sandwich immunoassay is described for determination of Escherichia coli O157:H7 ( E. coli O157:H7). Silica coated magnetite nanoparticles (Fe 3 O 4 ) were modified with primary antibody to capture E. coli O157:H7. Gold-platinum core/shell nanoparticles (Au@Pt NPs)... Full description

Journal Title: Mikrochimica acta (1966) 2018, Vol.185 (10), p.455-11
Main Author: Zhu, Fanjun
Other Authors: Zhao, Guangying , Dou, Wenchao
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Vienna: Springer Vienna
ID: ISSN: 0026-3672
Link: https://www.ncbi.nlm.nih.gov/pubmed/30215173
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title: Electrochemical sandwich immunoassay for Escherichia coli O157:H7 based on the use of magnetic nanoparticles and graphene functionalized with electrocatalytically active Au@Pt core/shell nanoparticles
format: Article
creator:
  • Zhu, Fanjun
  • Zhao, Guangying
  • Dou, Wenchao
subjects:
  • Analytical Chemistry
  • Antibodies
  • Bacteria
  • Catalysis
  • Characterization and Evaluation of Materials
  • Chemistry
  • Chemistry and Materials Science
  • Core-shell particles
  • E coli
  • Electrochemistry
  • Escherichia coli O157 - isolation & purification
  • Food
  • Gold
  • Gold - chemistry
  • Graphene
  • Graphite
  • Graphite - chemistry
  • Hydrogen peroxide
  • Hydrogen Peroxide - chemistry
  • Immunoassay
  • Immunoassay - methods
  • Iron oxides
  • Magnetite
  • Magnetite Nanoparticles - chemistry
  • Metal Nanoparticles - chemistry
  • Microengineering
  • Models, Molecular
  • Molecular Conformation
  • Nanochemistry
  • Nanoparticles
  • Nanotechnology
  • Original Paper
  • Oxidation-Reduction
  • Peroxidase
  • Phenothiazines - chemistry
  • Platinum
  • Platinum - chemistry
  • Safety and security measures
  • Signal to noise ratio
  • Silica
  • Silicon dioxide
  • Usage
  • Viral antibodies
ispartof: Mikrochimica acta (1966), 2018, Vol.185 (10), p.455-11
description: A highly sensitive electrochemical sandwich immunoassay is described for determination of Escherichia coli O157:H7 ( E. coli O157:H7). Silica coated magnetite nanoparticles (Fe 3 O 4 ) were modified with primary antibody to capture E. coli O157:H7. Gold-platinum core/shell nanoparticles (Au@Pt NPs) with different Pt shell thicknesses were prepared via changing the molar ratio of H 2 PtCl 6 to HAuCl 4 in the precursor solution. The optimized Au@Pt NPs exhibit enhanced activity in the electrocatalytic reduction of hydrogen peroxide (H 2 O 2 ). The Au@Pt NPs were modified with graphene that was functionalized with Neutral Red, and then used as an electrochemical label for secondary antibodies and horseradish peroxidase (HRP). The sandwich immunocomplexes were magnetically absorbed on a 4-channel screen printed carbon electrode. Due to the catalysis of the Au@Pt NPs and HRP, the signal is strongly amplified in the presence of H 2 O 2 when using thionine as the electron mediator. Under optimal conditions, the immunoassay has a linear response in the 4.0 × 10 2 to 4.0 × 10 8  CFU·mL −1 concentration range, with a limit of detection of 91 CFU·mL −1 (at an S/N ratio of 3). Graphical abstract Preparation of Au@Pt core/shell nanoparticles with different Pt shell thickness (A), rGO-NR (B), rGO-NR-Au@Pt-Ab 2 -HRP (C) and the preparation and the detection process of the immunoassay (D). rGO: reduced graphene oxide, GO: graphene oxide, NR: Neutral Red, HRP: horseradish peroxidase, AuNPs: gold nanoparticles, Fe 3 O 4 @SiO 2 : Silica coated magnetite nanoparticles, 4-SPCE: 4-channel screen printed carbon electrode.
language: eng
source:
identifier: ISSN: 0026-3672
fulltext: no_fulltext
issn:
  • 0026-3672
  • 1436-5073
url: Link


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titleElectrochemical sandwich immunoassay for Escherichia coli O157:H7 based on the use of magnetic nanoparticles and graphene functionalized with electrocatalytically active Au@Pt core/shell nanoparticles
creatorZhu, Fanjun ; Zhao, Guangying ; Dou, Wenchao
creatorcontribZhu, Fanjun ; Zhao, Guangying ; Dou, Wenchao
descriptionA highly sensitive electrochemical sandwich immunoassay is described for determination of Escherichia coli O157:H7 ( E. coli O157:H7). Silica coated magnetite nanoparticles (Fe 3 O 4 ) were modified with primary antibody to capture E. coli O157:H7. Gold-platinum core/shell nanoparticles (Au@Pt NPs) with different Pt shell thicknesses were prepared via changing the molar ratio of H 2 PtCl 6 to HAuCl 4 in the precursor solution. The optimized Au@Pt NPs exhibit enhanced activity in the electrocatalytic reduction of hydrogen peroxide (H 2 O 2 ). The Au@Pt NPs were modified with graphene that was functionalized with Neutral Red, and then used as an electrochemical label for secondary antibodies and horseradish peroxidase (HRP). The sandwich immunocomplexes were magnetically absorbed on a 4-channel screen printed carbon electrode. Due to the catalysis of the Au@Pt NPs and HRP, the signal is strongly amplified in the presence of H 2 O 2 when using thionine as the electron mediator. Under optimal conditions, the immunoassay has a linear response in the 4.0 × 10 2 to 4.0 × 10 8  CFU·mL −1 concentration range, with a limit of detection of 91 CFU·mL −1 (at an S/N ratio of 3). Graphical abstract Preparation of Au@Pt core/shell nanoparticles with different Pt shell thickness (A), rGO-NR (B), rGO-NR-Au@Pt-Ab 2 -HRP (C) and the preparation and the detection process of the immunoassay (D). rGO: reduced graphene oxide, GO: graphene oxide, NR: Neutral Red, HRP: horseradish peroxidase, AuNPs: gold nanoparticles, Fe 3 O 4 @SiO 2 : Silica coated magnetite nanoparticles, 4-SPCE: 4-channel screen printed carbon electrode.
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subjectAnalytical Chemistry ; Antibodies ; Bacteria ; Catalysis ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Core-shell particles ; E coli ; Electrochemistry ; Escherichia coli O157 - isolation & purification ; Food ; Gold ; Gold - chemistry ; Graphene ; Graphite ; Graphite - chemistry ; Hydrogen peroxide ; Hydrogen Peroxide - chemistry ; Immunoassay ; Immunoassay - methods ; Iron oxides ; Magnetite ; Magnetite Nanoparticles - chemistry ; Metal Nanoparticles - chemistry ; Microengineering ; Models, Molecular ; Molecular Conformation ; Nanochemistry ; Nanoparticles ; Nanotechnology ; Original Paper ; Oxidation-Reduction ; Peroxidase ; Phenothiazines - chemistry ; Platinum ; Platinum - chemistry ; Safety and security measures ; Signal to noise ratio ; Silica ; Silicon dioxide ; Usage ; Viral antibodies
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descriptionA highly sensitive electrochemical sandwich immunoassay is described for determination of Escherichia coli O157:H7 ( E. coli O157:H7). Silica coated magnetite nanoparticles (Fe 3 O 4 ) were modified with primary antibody to capture E. coli O157:H7. Gold-platinum core/shell nanoparticles (Au@Pt NPs) with different Pt shell thicknesses were prepared via changing the molar ratio of H 2 PtCl 6 to HAuCl 4 in the precursor solution. The optimized Au@Pt NPs exhibit enhanced activity in the electrocatalytic reduction of hydrogen peroxide (H 2 O 2 ). The Au@Pt NPs were modified with graphene that was functionalized with Neutral Red, and then used as an electrochemical label for secondary antibodies and horseradish peroxidase (HRP). The sandwich immunocomplexes were magnetically absorbed on a 4-channel screen printed carbon electrode. Due to the catalysis of the Au@Pt NPs and HRP, the signal is strongly amplified in the presence of H 2 O 2 when using thionine as the electron mediator. Under optimal conditions, the immunoassay has a linear response in the 4.0 × 10 2 to 4.0 × 10 8  CFU·mL −1 concentration range, with a limit of detection of 91 CFU·mL −1 (at an S/N ratio of 3). Graphical abstract Preparation of Au@Pt core/shell nanoparticles with different Pt shell thickness (A), rGO-NR (B), rGO-NR-Au@Pt-Ab 2 -HRP (C) and the preparation and the detection process of the immunoassay (D). rGO: reduced graphene oxide, GO: graphene oxide, NR: Neutral Red, HRP: horseradish peroxidase, AuNPs: gold nanoparticles, Fe 3 O 4 @SiO 2 : Silica coated magnetite nanoparticles, 4-SPCE: 4-channel screen printed carbon electrode.
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0Analytical Chemistry
1Antibodies
2Bacteria
3Catalysis
4Characterization and Evaluation of Materials
5Chemistry
6Chemistry and Materials Science
7Core-shell particles
8E coli
9Electrochemistry
10Escherichia coli O157 - isolation & purification
11Food
12Gold
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14Graphene
15Graphite
16Graphite - chemistry
17Hydrogen peroxide
18Hydrogen Peroxide - chemistry
19Immunoassay
20Immunoassay - methods
21Iron oxides
22Magnetite
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24Metal Nanoparticles - chemistry
25Microengineering
26Models, Molecular
27Molecular Conformation
28Nanochemistry
29Nanoparticles
30Nanotechnology
31Original Paper
32Oxidation-Reduction
33Peroxidase
34Phenothiazines - chemistry
35Platinum
36Platinum - chemistry
37Safety and security measures
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39Silica
40Silicon dioxide
41Usage
42Viral antibodies
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atitleElectrochemical sandwich immunoassay for Escherichia coli O157:H7 based on the use of magnetic nanoparticles and graphene functionalized with electrocatalytically active Au@Pt core/shell nanoparticles
jtitleMikrochimica acta (1966)
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abstractA highly sensitive electrochemical sandwich immunoassay is described for determination of Escherichia coli O157:H7 ( E. coli O157:H7). Silica coated magnetite nanoparticles (Fe 3 O 4 ) were modified with primary antibody to capture E. coli O157:H7. Gold-platinum core/shell nanoparticles (Au@Pt NPs) with different Pt shell thicknesses were prepared via changing the molar ratio of H 2 PtCl 6 to HAuCl 4 in the precursor solution. The optimized Au@Pt NPs exhibit enhanced activity in the electrocatalytic reduction of hydrogen peroxide (H 2 O 2 ). The Au@Pt NPs were modified with graphene that was functionalized with Neutral Red, and then used as an electrochemical label for secondary antibodies and horseradish peroxidase (HRP). The sandwich immunocomplexes were magnetically absorbed on a 4-channel screen printed carbon electrode. Due to the catalysis of the Au@Pt NPs and HRP, the signal is strongly amplified in the presence of H 2 O 2 when using thionine as the electron mediator. Under optimal conditions, the immunoassay has a linear response in the 4.0 × 10 2 to 4.0 × 10 8  CFU·mL −1 concentration range, with a limit of detection of 91 CFU·mL −1 (at an S/N ratio of 3). Graphical abstract Preparation of Au@Pt core/shell nanoparticles with different Pt shell thickness (A), rGO-NR (B), rGO-NR-Au@Pt-Ab 2 -HRP (C) and the preparation and the detection process of the immunoassay (D). rGO: reduced graphene oxide, GO: graphene oxide, NR: Neutral Red, HRP: horseradish peroxidase, AuNPs: gold nanoparticles, Fe 3 O 4 @SiO 2 : Silica coated magnetite nanoparticles, 4-SPCE: 4-channel screen printed carbon electrode.
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pmid30215173
doi10.1007/s00604-018-2984-2
orcididhttps://orcid.org/0000-0003-0999-012X