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Characterization of pre-existing and induced SARS-CoV-2-specific CD8 + T cells

Emerging data indicate that SARS-CoV-2-specific CD8 T cells targeting different viral proteins are detectable in up to 70% of convalescent individuals . However, very little information is currently available about the abundance, phenotype, functional capacity and fate of pre-existing and induced SA... Full description

Journal Title: Nature medicine 2021, Vol.27 (1), p.78-85
Main Author: Schulien, Isabel
Other Authors: Kemming, Janine , Oberhardt, Valerie , Wild, Katharina , Seidel, Lea M , Killmer, Saskia , Sagar , Daul, Franziska , Salvat Lago, Marilyn , Decker, Annegrit , Luxenburger, Hendrik , Binder, Benedikt , Bettinger, Dominik , Sogukpinar, Oezlem , Rieg, Siegbert , Panning, Marcus , Huzly, Daniela , Schwemmle, Martin , Kochs, Georg , Waller, Cornelius F , Nieters, Alexandra , Duerschmied, Daniel , Emmerich, Florian , Mei, Henrik E , Schulz, Axel Ronald , Llewellyn-Lacey, Sian , Price, David A , Boettler, Tobias , Bengsch, Bertram , Thimme, Robert , Hofmann, Maike , Neumann-Haefelin, Christoph
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: United States: Nature Publishing Group
ID: ISSN: 1078-8956
Link: https://www.ncbi.nlm.nih.gov/pubmed/33184509
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recordid: cdi_proquest_miscellaneous_2460768230
title: Characterization of pre-existing and induced SARS-CoV-2-specific CD8 + T cells
format: Article
creator:
  • Schulien, Isabel
  • Kemming, Janine
  • Oberhardt, Valerie
  • Wild, Katharina
  • Seidel, Lea M
  • Killmer, Saskia
  • Sagar
  • Daul, Franziska
  • Salvat Lago, Marilyn
  • Decker, Annegrit
  • Luxenburger, Hendrik
  • Binder, Benedikt
  • Bettinger, Dominik
  • Sogukpinar, Oezlem
  • Rieg, Siegbert
  • Panning, Marcus
  • Huzly, Daniela
  • Schwemmle, Martin
  • Kochs, Georg
  • Waller, Cornelius F
  • Nieters, Alexandra
  • Duerschmied, Daniel
  • Emmerich, Florian
  • Mei, Henrik E
  • Schulz, Axel Ronald
  • Llewellyn-Lacey, Sian
  • Price, David A
  • Boettler, Tobias
  • Bengsch, Bertram
  • Thimme, Robert
  • Hofmann, Maike
  • Neumann-Haefelin, Christoph
subjects:
  • Antibodies
  • Case-Control Studies
  • CD8 antigen
  • CD8-Positive T-Lymphocytes - immunology
  • Contraction
  • Convalescence
  • Coronavirus Nucleocapsid Proteins - chemistry
  • COVID-19 - blood
  • COVID-19 - immunology
  • Cross Reactions
  • Cross-Sectional Studies
  • Epitopes
  • Epitopes, T-Lymphocyte
  • Flow Cytometry
  • HLA-B Antigens - immunology
  • Humans
  • Immunologic Memory
  • Immunological memory
  • Infections
  • Influenza
  • Longitudinal Studies
  • Lymphocytes
  • Lymphocytes T
  • Major histocompatibility complex
  • Memory cells
  • Phenotypes
  • Phosphoproteins - chemistry
  • SARS-CoV-2 - physiology
  • Severe acute respiratory syndrome
  • Severe acute respiratory syndrome coronavirus 2
  • Spike Glycoprotein, Coronavirus - chemistry
  • Viral diseases
  • Viruses
ispartof: Nature medicine, 2021, Vol.27 (1), p.78-85
description: Emerging data indicate that SARS-CoV-2-specific CD8 T cells targeting different viral proteins are detectable in up to 70% of convalescent individuals . However, very little information is currently available about the abundance, phenotype, functional capacity and fate of pre-existing and induced SARS-CoV-2-specific CD8 T cell responses during the natural course of SARS-CoV-2 infection. Here, we define a set of optimal and dominant SARS-CoV-2-specific CD8 T cell epitopes. We also perform a high-resolution ex vivo analysis of pre-existing and induced SARS-CoV-2-specific CD8 T cells, applying peptide-loaded major histocompatibility complex class I (pMHCI) tetramer technology. We observe rapid induction, prolonged contraction and emergence of heterogeneous and functionally competent cross-reactive and induced memory CD8 T cell responses in cross-sectionally analyzed individuals with mild disease following SARS-CoV-2 infection and three individuals longitudinally assessed for their T cells pre- and post-SARS-CoV-2 infection. SARS-CoV-2-specific memory CD8 T cells exhibited functional characteristics comparable to influenza-specific CD8 T cells and were detectable in SARS-CoV-2 convalescent individuals who were seronegative for anti-SARS-CoV-2 antibodies targeting spike (S) and nucleoprotein (N). These results define cross-reactive and induced SARS-CoV-2-specific CD8 T cell responses as potentially important determinants of immune protection in mild SARS-CoV-2 infection.
language: eng
source:
identifier: ISSN: 1078-8956
fulltext: no_fulltext
issn:
  • 1078-8956
  • 1546-170X
url: Link


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titleCharacterization of pre-existing and induced SARS-CoV-2-specific CD8 + T cells
creatorSchulien, Isabel ; Kemming, Janine ; Oberhardt, Valerie ; Wild, Katharina ; Seidel, Lea M ; Killmer, Saskia ; Sagar ; Daul, Franziska ; Salvat Lago, Marilyn ; Decker, Annegrit ; Luxenburger, Hendrik ; Binder, Benedikt ; Bettinger, Dominik ; Sogukpinar, Oezlem ; Rieg, Siegbert ; Panning, Marcus ; Huzly, Daniela ; Schwemmle, Martin ; Kochs, Georg ; Waller, Cornelius F ; Nieters, Alexandra ; Duerschmied, Daniel ; Emmerich, Florian ; Mei, Henrik E ; Schulz, Axel Ronald ; Llewellyn-Lacey, Sian ; Price, David A ; Boettler, Tobias ; Bengsch, Bertram ; Thimme, Robert ; Hofmann, Maike ; Neumann-Haefelin, Christoph
creatorcontribSchulien, Isabel ; Kemming, Janine ; Oberhardt, Valerie ; Wild, Katharina ; Seidel, Lea M ; Killmer, Saskia ; Sagar ; Daul, Franziska ; Salvat Lago, Marilyn ; Decker, Annegrit ; Luxenburger, Hendrik ; Binder, Benedikt ; Bettinger, Dominik ; Sogukpinar, Oezlem ; Rieg, Siegbert ; Panning, Marcus ; Huzly, Daniela ; Schwemmle, Martin ; Kochs, Georg ; Waller, Cornelius F ; Nieters, Alexandra ; Duerschmied, Daniel ; Emmerich, Florian ; Mei, Henrik E ; Schulz, Axel Ronald ; Llewellyn-Lacey, Sian ; Price, David A ; Boettler, Tobias ; Bengsch, Bertram ; Thimme, Robert ; Hofmann, Maike ; Neumann-Haefelin, Christoph
descriptionEmerging data indicate that SARS-CoV-2-specific CD8 T cells targeting different viral proteins are detectable in up to 70% of convalescent individuals . However, very little information is currently available about the abundance, phenotype, functional capacity and fate of pre-existing and induced SARS-CoV-2-specific CD8 T cell responses during the natural course of SARS-CoV-2 infection. Here, we define a set of optimal and dominant SARS-CoV-2-specific CD8 T cell epitopes. We also perform a high-resolution ex vivo analysis of pre-existing and induced SARS-CoV-2-specific CD8 T cells, applying peptide-loaded major histocompatibility complex class I (pMHCI) tetramer technology. We observe rapid induction, prolonged contraction and emergence of heterogeneous and functionally competent cross-reactive and induced memory CD8 T cell responses in cross-sectionally analyzed individuals with mild disease following SARS-CoV-2 infection and three individuals longitudinally assessed for their T cells pre- and post-SARS-CoV-2 infection. SARS-CoV-2-specific memory CD8 T cells exhibited functional characteristics comparable to influenza-specific CD8 T cells and were detectable in SARS-CoV-2 convalescent individuals who were seronegative for anti-SARS-CoV-2 antibodies targeting spike (S) and nucleoprotein (N). These results define cross-reactive and induced SARS-CoV-2-specific CD8 T cell responses as potentially important determinants of immune protection in mild SARS-CoV-2 infection.
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subjectAntibodies ; Case-Control Studies ; CD8 antigen ; CD8-Positive T-Lymphocytes - immunology ; Contraction ; Convalescence ; Coronavirus Nucleocapsid Proteins - chemistry ; COVID-19 - blood ; COVID-19 - immunology ; Cross Reactions ; Cross-Sectional Studies ; Epitopes ; Epitopes, T-Lymphocyte ; Flow Cytometry ; HLA-B Antigens - immunology ; Humans ; Immunologic Memory ; Immunological memory ; Infections ; Influenza ; Longitudinal Studies ; Lymphocytes ; Lymphocytes T ; Major histocompatibility complex ; Memory cells ; Phenotypes ; Phosphoproteins - chemistry ; SARS-CoV-2 - physiology ; Severe acute respiratory syndrome ; Severe acute respiratory syndrome coronavirus 2 ; Spike Glycoprotein, Coronavirus - chemistry ; Viral diseases ; Viruses
ispartofNature medicine, 2021, Vol.27 (1), p.78-85
rightsThe Author(s), under exclusive licence to Springer Nature America, Inc. 2020.
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19Waller, Cornelius F
20Nieters, Alexandra
21Duerschmied, Daniel
22Emmerich, Florian
23Mei, Henrik E
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26Price, David A
27Boettler, Tobias
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31Neumann-Haefelin, Christoph
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0Characterization of pre-existing and induced SARS-CoV-2-specific CD8 + T cells
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descriptionEmerging data indicate that SARS-CoV-2-specific CD8 T cells targeting different viral proteins are detectable in up to 70% of convalescent individuals . However, very little information is currently available about the abundance, phenotype, functional capacity and fate of pre-existing and induced SARS-CoV-2-specific CD8 T cell responses during the natural course of SARS-CoV-2 infection. Here, we define a set of optimal and dominant SARS-CoV-2-specific CD8 T cell epitopes. We also perform a high-resolution ex vivo analysis of pre-existing and induced SARS-CoV-2-specific CD8 T cells, applying peptide-loaded major histocompatibility complex class I (pMHCI) tetramer technology. We observe rapid induction, prolonged contraction and emergence of heterogeneous and functionally competent cross-reactive and induced memory CD8 T cell responses in cross-sectionally analyzed individuals with mild disease following SARS-CoV-2 infection and three individuals longitudinally assessed for their T cells pre- and post-SARS-CoV-2 infection. SARS-CoV-2-specific memory CD8 T cells exhibited functional characteristics comparable to influenza-specific CD8 T cells and were detectable in SARS-CoV-2 convalescent individuals who were seronegative for anti-SARS-CoV-2 antibodies targeting spike (S) and nucleoprotein (N). These results define cross-reactive and induced SARS-CoV-2-specific CD8 T cell responses as potentially important determinants of immune protection in mild SARS-CoV-2 infection.
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11Epitopes
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15Humans
16Immunologic Memory
17Immunological memory
18Infections
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20Longitudinal Studies
21Lymphocytes
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23Major histocompatibility complex
24Memory cells
25Phenotypes
26Phosphoproteins - chemistry
27SARS-CoV-2 - physiology
28Severe acute respiratory syndrome
29Severe acute respiratory syndrome coronavirus 2
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31Viral diseases
32Viruses
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titleCharacterization of pre-existing and induced SARS-CoV-2-specific CD8 + T cells
authorSchulien, Isabel ; Kemming, Janine ; Oberhardt, Valerie ; Wild, Katharina ; Seidel, Lea M ; Killmer, Saskia ; Sagar ; Daul, Franziska ; Salvat Lago, Marilyn ; Decker, Annegrit ; Luxenburger, Hendrik ; Binder, Benedikt ; Bettinger, Dominik ; Sogukpinar, Oezlem ; Rieg, Siegbert ; Panning, Marcus ; Huzly, Daniela ; Schwemmle, Martin ; Kochs, Georg ; Waller, Cornelius F ; Nieters, Alexandra ; Duerschmied, Daniel ; Emmerich, Florian ; Mei, Henrik E ; Schulz, Axel Ronald ; Llewellyn-Lacey, Sian ; Price, David A ; Boettler, Tobias ; Bengsch, Bertram ; Thimme, Robert ; Hofmann, Maike ; Neumann-Haefelin, Christoph
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30Spike Glycoprotein, Coronavirus - chemistry
31Viral diseases
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abstractEmerging data indicate that SARS-CoV-2-specific CD8 T cells targeting different viral proteins are detectable in up to 70% of convalescent individuals . However, very little information is currently available about the abundance, phenotype, functional capacity and fate of pre-existing and induced SARS-CoV-2-specific CD8 T cell responses during the natural course of SARS-CoV-2 infection. Here, we define a set of optimal and dominant SARS-CoV-2-specific CD8 T cell epitopes. We also perform a high-resolution ex vivo analysis of pre-existing and induced SARS-CoV-2-specific CD8 T cells, applying peptide-loaded major histocompatibility complex class I (pMHCI) tetramer technology. We observe rapid induction, prolonged contraction and emergence of heterogeneous and functionally competent cross-reactive and induced memory CD8 T cell responses in cross-sectionally analyzed individuals with mild disease following SARS-CoV-2 infection and three individuals longitudinally assessed for their T cells pre- and post-SARS-CoV-2 infection. SARS-CoV-2-specific memory CD8 T cells exhibited functional characteristics comparable to influenza-specific CD8 T cells and were detectable in SARS-CoV-2 convalescent individuals who were seronegative for anti-SARS-CoV-2 antibodies targeting spike (S) and nucleoprotein (N). These results define cross-reactive and induced SARS-CoV-2-specific CD8 T cell responses as potentially important determinants of immune protection in mild SARS-CoV-2 infection.
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