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Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Method to Distinguish Three Mealybug Groups Within the Planococcus citri-P. minor Species Complex (Hemiptera: Coccoidea: Pseudococcidae)

The mealybug species Planococcus citri (Risso) and Planococcus minor (Maskell) (Hemiptera: Coccoidea: Pseudococcidae) have special significance to U.S. quarantine and U.S. agriculture. Commonly intercepted at U.S. ports-of-entry, they are difficult to identify based on morphological characters. This... Full description

Journal Title: Journal of economic entomology 2009, Vol.102 (1), p.8-12
Main Author: Rung, A
Other Authors: Miller, D. R , Scheffer, S. J
Format: Electronic Article Electronic Article
Language: English
Subjects:
PCR
Publisher: Lanham, MD: Entomological Society of America
ID: ISSN: 0022-0493
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recordid: cdi_proquest_miscellaneous_66984721
title: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Method to Distinguish Three Mealybug Groups Within the Planococcus citri-P. minor Species Complex (Hemiptera: Coccoidea: Pseudococcidae)
format: Article
creator:
  • Rung, A
  • Miller, D. R
  • Scheffer, S. J
subjects:
  • Animals
  • Biological and medical sciences
  • Coccoidea
  • Control
  • diagnostics
  • Fundamental and applied biological sciences. Psychology
  • Generalities
  • Hemiptera
  • Hemiptera - classification
  • Hemiptera - genetics
  • PCR
  • Phytopathology. Animal pests. Plant and forest protection
  • Planococcus
  • Planococcus citri
  • Planococcus minor
  • Polymerase Chain Reaction - methods
  • Polymorphism, Restriction Fragment Length
  • Protozoa. Invertebrates
  • Pseudococcidae
  • RAPID COMMUNICATIONS
  • restriction fragment length polymorphism
ispartof: Journal of economic entomology, 2009, Vol.102 (1), p.8-12
description: The mealybug species Planococcus citri (Risso) and Planococcus minor (Maskell) (Hemiptera: Coccoidea: Pseudococcidae) have special significance to U.S. quarantine and U.S. agriculture. Commonly intercepted at U.S. ports-of-entry, they are difficult to identify based on morphological characters. This study presents a molecular method for distinguishing P. citri, P. minor, and a genetically distinct group that is morphologically identical to P. citri, from Hawaii. This method uses polymerase chain reaction (PCR) followed by restriction fragment polymorphism analysis (RFLP) using the restriction enzymes BspHl, BsmHl, and HpHl. The resulting band patterns can be visualized in a 2% agarose gel and are sufficient to differentiate between the three entities mentioned above. PCR-RFLP diagnostics can be used for all life stages and is cheaper and faster than DNA sequencing.
language: eng
source:
identifier: ISSN: 0022-0493
fulltext: no_fulltext
issn:
  • 0022-0493
  • 1938-291X
url: Link


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titlePolymerase Chain Reaction-Restriction Fragment Length Polymorphism Method to Distinguish Three Mealybug Groups Within the Planococcus citri-P. minor Species Complex (Hemiptera: Coccoidea: Pseudococcidae)
creatorRung, A ; Miller, D. R ; Scheffer, S. J
creatorcontribRung, A ; Miller, D. R ; Scheffer, S. J
descriptionThe mealybug species Planococcus citri (Risso) and Planococcus minor (Maskell) (Hemiptera: Coccoidea: Pseudococcidae) have special significance to U.S. quarantine and U.S. agriculture. Commonly intercepted at U.S. ports-of-entry, they are difficult to identify based on morphological characters. This study presents a molecular method for distinguishing P. citri, P. minor, and a genetically distinct group that is morphologically identical to P. citri, from Hawaii. This method uses polymerase chain reaction (PCR) followed by restriction fragment polymorphism analysis (RFLP) using the restriction enzymes BspHl, BsmHl, and HpHl. The resulting band patterns can be visualized in a 2% agarose gel and are sufficient to differentiate between the three entities mentioned above. PCR-RFLP diagnostics can be used for all life stages and is cheaper and faster than DNA sequencing.
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subjectAnimals ; Biological and medical sciences ; Coccoidea ; Control ; diagnostics ; Fundamental and applied biological sciences. Psychology ; Generalities ; Hemiptera ; Hemiptera - classification ; Hemiptera - genetics ; PCR ; Phytopathology. Animal pests. Plant and forest protection ; Planococcus ; Planococcus citri ; Planococcus minor ; Polymerase Chain Reaction - methods ; Polymorphism, Restriction Fragment Length ; Protozoa. Invertebrates ; Pseudococcidae ; RAPID COMMUNICATIONS ; restriction fragment length polymorphism
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titlePolymerase Chain Reaction-Restriction Fragment Length Polymorphism Method to Distinguish Three Mealybug Groups Within the Planococcus citri-P. minor Species Complex (Hemiptera: Coccoidea: Pseudococcidae)
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abstractThe mealybug species Planococcus citri (Risso) and Planococcus minor (Maskell) (Hemiptera: Coccoidea: Pseudococcidae) have special significance to U.S. quarantine and U.S. agriculture. Commonly intercepted at U.S. ports-of-entry, they are difficult to identify based on morphological characters. This study presents a molecular method for distinguishing P. citri, P. minor, and a genetically distinct group that is morphologically identical to P. citri, from Hawaii. This method uses polymerase chain reaction (PCR) followed by restriction fragment polymorphism analysis (RFLP) using the restriction enzymes BspHl, BsmHl, and HpHl. The resulting band patterns can be visualized in a 2% agarose gel and are sufficient to differentiate between the three entities mentioned above. PCR-RFLP diagnostics can be used for all life stages and is cheaper and faster than DNA sequencing.
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doi10.1603/029.102.0102
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