schliessen

Filtern

 

Bibliotheken

In vitro and animal study of novel nano-hydroxyapatite/poly(epsilon-caprolactone) composite scaffolds fabricated by layer manufacturing process

The purpose of this study was to propose a computer-controllable scaffold structure made by a layer manufacturing process (LMP) with addition of nano- or micro-sized particles and to investigate the effects of particle size in vitro. In addition, the superiority of this LMP method over the conventio... Full description

Journal Title: Tissue engineering. Part A 2009-05, Vol.15 (5), p.977-989
Main Author: Heo, Su-Jin
Other Authors: Kim, Seung-Eon , Wei, Jie , Kim, Dong Hwa , Hyun, Young-Taek , Yun, Hui-Suk , Kim, Hyung Keun , Yoon, Taek Rim , Kim, Su-Hyang , Park, Su-A , Shin, Ji Won , Shin, Jung-Woog
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: United States: Mary Ann Liebert, Inc
ID: ISSN: 1937-3341
Link: https://www.ncbi.nlm.nih.gov/pubmed/18803480
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_proquest_miscellaneous_67195272
title: In vitro and animal study of novel nano-hydroxyapatite/poly(epsilon-caprolactone) composite scaffolds fabricated by layer manufacturing process
format: Article
creator:
  • Heo, Su-Jin
  • Kim, Seung-Eon
  • Wei, Jie
  • Kim, Dong Hwa
  • Hyun, Young-Taek
  • Yun, Hui-Suk
  • Kim, Hyung Keun
  • Yoon, Taek Rim
  • Kim, Su-Hyang
  • Park, Su-A
  • Shin, Ji Won
  • Shin, Jung-Woog
subjects:
  • Alkaline Phosphatase - metabolism
  • Animal experimentation
  • Animals
  • Biomedical materials
  • Bone Regeneration
  • Bone Substitutes
  • Calcium - metabolism
  • Cell Adhesion
  • Cell Differentiation
  • Cell Proliferation
  • Composite materials
  • Compressive Strength
  • Health aspects
  • Hydroxyapatites
  • Hydroxylapatite
  • Mesenchymal Stromal Cells - cytology
  • Mesenchymal Stromal Cells - metabolism
  • Methods
  • Microscopy, Electron, Scanning
  • Nanocomposites
  • Nanomaterials
  • Nanotechnology
  • Particle Size
  • Physiological aspects
  • Polyesters
  • Rabbits
  • Stem cells
  • Tibia - injuries
  • Tibia - metabolism
  • Tibia - physiology
  • Tissue engineering
  • Tissue Engineering - instrumentation
  • Tissue Engineering - methods
  • Tissue Scaffolds
  • Tomography, X-Ray Computed
  • Usage
ispartof: Tissue engineering. Part A, 2009-05, Vol.15 (5), p.977-989
description: The purpose of this study was to propose a computer-controllable scaffold structure made by a layer manufacturing process (LMP) with addition of nano- or micro-sized particles and to investigate the effects of particle size in vitro. In addition, the superiority of this LMP method over the conventional scaffolds made by salt leaching and gas forming process was investigated through animal study. Using the LMP, we have created a new nano-sized hydroxyapatite/poly(epsilon-caprolactone) composite (n-HPC) scaffold and a micro-sized hydroxyapatite/poly(epsilon-caprolactone) composite (m-HPC) scaffold for bone tissue engineering applications. The scaffold macropores were well interconnected, with a porosity of 73% and a pore size of 500 microm. The compressive modulus of the n-HPC and m-HPC scaffolds was 6.76 and 3.18 MPa, respectively. We compared the cellular responses to the two kinds of scaffolds. Both n-HPC and m-HPC exhibited good in vitro biocompatibility. Attachment and proliferation of mesenchymal stem cells were better on the n-HPC than on the m-HPC scaffold. Moreover, significantly higher alkaline phosphatase activity and calcium content were observed on the n-HPC than on the m-HPC scaffold. In an animal study, the LMP scaffolds enhanced bone formation, owing to their well-interconnected pores. Radiological and histological examinations confirmed that the new bony tissue had grown easily into the entire n-HPC scaffold fabricated by LMP. We suggest that the well-interconnected pores in the LMP scaffolds might encourage cell attachment, proliferation, and migration to stimulate cell functions, thus enhancing bone formation in the LMP scaffolds. This study shows that bioactive and biocompatible n-HPC composite scaffolds prepared using an LMP have potential applications in bone tissue engineering.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 1937-3341
fulltext: fulltext
issn:
  • 1937-3341
  • 1937-335X
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.37016
LOCALfalse
PrimoNMBib
record
control
sourceidgale_proqu
recordidTN_cdi_proquest_miscellaneous_67195272
sourceformatXML
sourcesystemPC
galeidA205747183
sourcerecordidA205747183
originalsourceidFETCH-LOGICAL-c414t-c530b0bbe1b05fd6139e16dfd94d9eb40fea99b2d17e6dc166020083faad084c3
addsrcrecordideNqFkt1qFjEQhhdRbK1egCcSEUQP9muyP9nNYSn-FAqeKHgWZpNJTckma5It7lV4y-azH4pFlDAkhOd9M5mZqnrK6I7RUZxm9LuMsGsoHXeUCXqvOmaiHeq27T_f_3Xu2FH1KKVrSjnlw_CwOmLjSNtupMfV9wtPbmyOgYDXJewMjqS86o0EQ3y4QUc8-FB_2XQM3zZYINuMp0tw2ytcknXB1wqWGByoHDy-JirMS0gFIkmBMcHpRAxM0SrIqMm0EQcbRjKDX00RrdH6K1IcFKb0uHpgwCV8cthPqk9v33w8f19ffnh3cX52WauOdblWfUsnOk3IJtobzVkrkHFttOi0wKmjBkGIqdFsQK4V45zui9QaAE3HTrUn1ctb3_Lu1xVTlrNNCp0Dj2FNkg9M9M3Q_BdsKGdc9LyAz--A12GNvnxCjqLkN_w0e3HLXIFDab0JOYLaG8qzhvZDN7CxLdTuL1RZGmerSo2NLfd_CNitQMWQUkQjl1gaGTfJqNxPiiyTUgLkvghyPylF8-yQ7jrNqH8rDqNRgOGOqbK5ND_4ko11_7D-AUlS0GU
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid89139772
display
typearticle
titleIn vitro and animal study of novel nano-hydroxyapatite/poly(epsilon-caprolactone) composite scaffolds fabricated by layer manufacturing process
sourceAlma/SFX Local Collection
creatorHeo, Su-Jin ; Kim, Seung-Eon ; Wei, Jie ; Kim, Dong Hwa ; Hyun, Young-Taek ; Yun, Hui-Suk ; Kim, Hyung Keun ; Yoon, Taek Rim ; Kim, Su-Hyang ; Park, Su-A ; Shin, Ji Won ; Shin, Jung-Woog
creatorcontribHeo, Su-Jin ; Kim, Seung-Eon ; Wei, Jie ; Kim, Dong Hwa ; Hyun, Young-Taek ; Yun, Hui-Suk ; Kim, Hyung Keun ; Yoon, Taek Rim ; Kim, Su-Hyang ; Park, Su-A ; Shin, Ji Won ; Shin, Jung-Woog
descriptionThe purpose of this study was to propose a computer-controllable scaffold structure made by a layer manufacturing process (LMP) with addition of nano- or micro-sized particles and to investigate the effects of particle size in vitro. In addition, the superiority of this LMP method over the conventional scaffolds made by salt leaching and gas forming process was investigated through animal study. Using the LMP, we have created a new nano-sized hydroxyapatite/poly(epsilon-caprolactone) composite (n-HPC) scaffold and a micro-sized hydroxyapatite/poly(epsilon-caprolactone) composite (m-HPC) scaffold for bone tissue engineering applications. The scaffold macropores were well interconnected, with a porosity of 73% and a pore size of 500 microm. The compressive modulus of the n-HPC and m-HPC scaffolds was 6.76 and 3.18 MPa, respectively. We compared the cellular responses to the two kinds of scaffolds. Both n-HPC and m-HPC exhibited good in vitro biocompatibility. Attachment and proliferation of mesenchymal stem cells were better on the n-HPC than on the m-HPC scaffold. Moreover, significantly higher alkaline phosphatase activity and calcium content were observed on the n-HPC than on the m-HPC scaffold. In an animal study, the LMP scaffolds enhanced bone formation, owing to their well-interconnected pores. Radiological and histological examinations confirmed that the new bony tissue had grown easily into the entire n-HPC scaffold fabricated by LMP. We suggest that the well-interconnected pores in the LMP scaffolds might encourage cell attachment, proliferation, and migration to stimulate cell functions, thus enhancing bone formation in the LMP scaffolds. This study shows that bioactive and biocompatible n-HPC composite scaffolds prepared using an LMP have potential applications in bone tissue engineering.
identifier
0ISSN: 1937-3341
1EISSN: 1937-335X
2DOI: 10.1089/ten.tea.2008.0190
3PMID: 18803480
languageeng
publisherUnited States: Mary Ann Liebert, Inc
subjectAlkaline Phosphatase - metabolism ; Animal experimentation ; Animals ; Biomedical materials ; Bone Regeneration ; Bone Substitutes ; Calcium - metabolism ; Cell Adhesion ; Cell Differentiation ; Cell Proliferation ; Composite materials ; Compressive Strength ; Health aspects ; Hydroxyapatites ; Hydroxylapatite ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - metabolism ; Methods ; Microscopy, Electron, Scanning ; Nanocomposites ; Nanomaterials ; Nanotechnology ; Particle Size ; Physiological aspects ; Polyesters ; Rabbits ; Stem cells ; Tibia - injuries ; Tibia - metabolism ; Tibia - physiology ; Tissue engineering ; Tissue Engineering - instrumentation ; Tissue Engineering - methods ; Tissue Scaffolds ; Tomography, X-Ray Computed ; Usage
ispartofTissue engineering. Part A, 2009-05, Vol.15 (5), p.977-989
rights
0COPYRIGHT 2009 Mary Ann Liebert, Inc.
1(©) Copyright 2009, Mary Ann Liebert, Inc.
lds50peer_reviewed
citedbyFETCH-LOGICAL-c414t-c530b0bbe1b05fd6139e16dfd94d9eb40fea99b2d17e6dc166020083faad084c3
citesFETCH-LOGICAL-c414t-c530b0bbe1b05fd6139e16dfd94d9eb40fea99b2d17e6dc166020083faad084c3
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18803480$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Heo, Su-Jin
1Kim, Seung-Eon
2Wei, Jie
3Kim, Dong Hwa
4Hyun, Young-Taek
5Yun, Hui-Suk
6Kim, Hyung Keun
7Yoon, Taek Rim
8Kim, Su-Hyang
9Park, Su-A
10Shin, Ji Won
11Shin, Jung-Woog
title
0In vitro and animal study of novel nano-hydroxyapatite/poly(epsilon-caprolactone) composite scaffolds fabricated by layer manufacturing process
1Tissue engineering. Part A
addtitleTissue Eng Part A
descriptionThe purpose of this study was to propose a computer-controllable scaffold structure made by a layer manufacturing process (LMP) with addition of nano- or micro-sized particles and to investigate the effects of particle size in vitro. In addition, the superiority of this LMP method over the conventional scaffolds made by salt leaching and gas forming process was investigated through animal study. Using the LMP, we have created a new nano-sized hydroxyapatite/poly(epsilon-caprolactone) composite (n-HPC) scaffold and a micro-sized hydroxyapatite/poly(epsilon-caprolactone) composite (m-HPC) scaffold for bone tissue engineering applications. The scaffold macropores were well interconnected, with a porosity of 73% and a pore size of 500 microm. The compressive modulus of the n-HPC and m-HPC scaffolds was 6.76 and 3.18 MPa, respectively. We compared the cellular responses to the two kinds of scaffolds. Both n-HPC and m-HPC exhibited good in vitro biocompatibility. Attachment and proliferation of mesenchymal stem cells were better on the n-HPC than on the m-HPC scaffold. Moreover, significantly higher alkaline phosphatase activity and calcium content were observed on the n-HPC than on the m-HPC scaffold. In an animal study, the LMP scaffolds enhanced bone formation, owing to their well-interconnected pores. Radiological and histological examinations confirmed that the new bony tissue had grown easily into the entire n-HPC scaffold fabricated by LMP. We suggest that the well-interconnected pores in the LMP scaffolds might encourage cell attachment, proliferation, and migration to stimulate cell functions, thus enhancing bone formation in the LMP scaffolds. This study shows that bioactive and biocompatible n-HPC composite scaffolds prepared using an LMP have potential applications in bone tissue engineering.
subject
0Alkaline Phosphatase - metabolism
1Animal experimentation
2Animals
3Biomedical materials
4Bone Regeneration
5Bone Substitutes
6Calcium - metabolism
7Cell Adhesion
8Cell Differentiation
9Cell Proliferation
10Composite materials
11Compressive Strength
12Health aspects
13Hydroxyapatites
14Hydroxylapatite
15Mesenchymal Stromal Cells - cytology
16Mesenchymal Stromal Cells - metabolism
17Methods
18Microscopy, Electron, Scanning
19Nanocomposites
20Nanomaterials
21Nanotechnology
22Particle Size
23Physiological aspects
24Polyesters
25Rabbits
26Stem cells
27Tibia - injuries
28Tibia - metabolism
29Tibia - physiology
30Tissue engineering
31Tissue Engineering - instrumentation
32Tissue Engineering - methods
33Tissue Scaffolds
34Tomography, X-Ray Computed
35Usage
issn
01937-3341
11937-335X
fulltexttrue
rsrctypearticle
creationdate2009
recordtypearticle
recordideNqFkt1qFjEQhhdRbK1egCcSEUQP9muyP9nNYSn-FAqeKHgWZpNJTckma5It7lV4y-azH4pFlDAkhOd9M5mZqnrK6I7RUZxm9LuMsGsoHXeUCXqvOmaiHeq27T_f_3Xu2FH1KKVrSjnlw_CwOmLjSNtupMfV9wtPbmyOgYDXJewMjqS86o0EQ3y4QUc8-FB_2XQM3zZYINuMp0tw2ytcknXB1wqWGByoHDy-JirMS0gFIkmBMcHpRAxM0SrIqMm0EQcbRjKDX00RrdH6K1IcFKb0uHpgwCV8cthPqk9v33w8f19ffnh3cX52WauOdblWfUsnOk3IJtobzVkrkHFttOi0wKmjBkGIqdFsQK4V45zui9QaAE3HTrUn1ctb3_Lu1xVTlrNNCp0Dj2FNkg9M9M3Q_BdsKGdc9LyAz--A12GNvnxCjqLkN_w0e3HLXIFDab0JOYLaG8qzhvZDN7CxLdTuL1RZGmerSo2NLfd_CNitQMWQUkQjl1gaGTfJqNxPiiyTUgLkvghyPylF8-yQ7jrNqH8rDqNRgOGOqbK5ND_4ko11_7D-AUlS0GU
startdate200905
enddate200905
creator
0Heo, Su-Jin
1Kim, Seung-Eon
2Wei, Jie
3Kim, Dong Hwa
4Hyun, Young-Taek
5Yun, Hui-Suk
6Kim, Hyung Keun
7Yoon, Taek Rim
8Kim, Su-Hyang
9Park, Su-A
10Shin, Ji Won
11Shin, Jung-Woog
generalMary Ann Liebert, Inc
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
83V.
97QP
107T5
117TK
127TM
137TO
147X7
157XB
1688A
1788E
1888I
198FE
208FH
218FI
228FJ
238FK
24ABUWG
25AZQEC
26BBNVY
27BENPR
28BHPHI
29DWQXO
30FYUFA
31GHDGH
32GNUQQ
33H94
34HCIFZ
35K9.
36LK8
37M0S
38M1P
39M2P
40M7P
41PQEST
42PQQKQ
43PQUKI
44PRINS
45Q9U
467QO
478FD
48FR3
49P64
507X8
sort
creationdate200905
titleIn vitro and animal study of novel nano-hydroxyapatite/poly(epsilon-caprolactone) composite scaffolds fabricated by layer manufacturing process
authorHeo, Su-Jin ; Kim, Seung-Eon ; Wei, Jie ; Kim, Dong Hwa ; Hyun, Young-Taek ; Yun, Hui-Suk ; Kim, Hyung Keun ; Yoon, Taek Rim ; Kim, Su-Hyang ; Park, Su-A ; Shin, Ji Won ; Shin, Jung-Woog
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-c414t-c530b0bbe1b05fd6139e16dfd94d9eb40fea99b2d17e6dc166020083faad084c3
rsrctypearticles
prefilterarticles
languageeng
creationdate2009
topic
0Alkaline Phosphatase - metabolism
1Animal experimentation
2Animals
3Biomedical materials
4Bone Regeneration
5Bone Substitutes
6Calcium - metabolism
7Cell Adhesion
8Cell Differentiation
9Cell Proliferation
10Composite materials
11Compressive Strength
12Health aspects
13Hydroxyapatites
14Hydroxylapatite
15Mesenchymal Stromal Cells - cytology
16Mesenchymal Stromal Cells - metabolism
17Methods
18Microscopy, Electron, Scanning
19Nanocomposites
20Nanomaterials
21Nanotechnology
22Particle Size
23Physiological aspects
24Polyesters
25Rabbits
26Stem cells
27Tibia - injuries
28Tibia - metabolism
29Tibia - physiology
30Tissue engineering
31Tissue Engineering - instrumentation
32Tissue Engineering - methods
33Tissue Scaffolds
34Tomography, X-Ray Computed
35Usage
toplevel
0peer_reviewed
1online_resources
creatorcontrib
0Heo, Su-Jin
1Kim, Seung-Eon
2Wei, Jie
3Kim, Dong Hwa
4Hyun, Young-Taek
5Yun, Hui-Suk
6Kim, Hyung Keun
7Yoon, Taek Rim
8Kim, Su-Hyang
9Park, Su-A
10Shin, Ji Won
11Shin, Jung-Woog
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7ProQuest Central (Corporate)
8Calcium & Calcified Tissue Abstracts
9Immunology Abstracts
10Neurosciences Abstracts
11Nucleic Acids Abstracts
12Oncogenes and Growth Factors Abstracts
13Health & Medical Collection
14ProQuest Central (purchase pre-March 2016)
15Biology Database (Alumni Edition)
16Medical Database (Alumni Edition)
17Science Database (Alumni Edition)
18ProQuest SciTech Collection
19ProQuest Natural Science Collection
20Hospital Premium Collection
21Hospital Premium Collection (Alumni Edition)
22ProQuest Central (Alumni) (purchase pre-March 2016)
23ProQuest Central (Alumni Edition)
24ProQuest Central Essentials
25Biological Science Collection
26ProQuest Central
27Natural Science Collection
28ProQuest Central Korea
29Health Research Premium Collection
30Health Research Premium Collection (Alumni)
31ProQuest Central Student
32AIDS and Cancer Research Abstracts
33SciTech Premium Collection
34ProQuest Health & Medical Complete (Alumni)
35ProQuest Biological Science Collection
36Health & Medical Collection (Alumni Edition)
37Medical Database
38Science Database
39Biological Science Database
40ProQuest One Academic Eastern Edition
41ProQuest One Academic
42ProQuest One Academic UKI Edition
43ProQuest Central China
44ProQuest Central Basic
45Biotechnology Research Abstracts
46Technology Research Database
47Engineering Research Database
48Biotechnology and BioEngineering Abstracts
49MEDLINE - Academic
jtitleTissue engineering. Part A
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Heo, Su-Jin
1Kim, Seung-Eon
2Wei, Jie
3Kim, Dong Hwa
4Hyun, Young-Taek
5Yun, Hui-Suk
6Kim, Hyung Keun
7Yoon, Taek Rim
8Kim, Su-Hyang
9Park, Su-A
10Shin, Ji Won
11Shin, Jung-Woog
formatjournal
genrearticle
ristypeJOUR
atitleIn vitro and animal study of novel nano-hydroxyapatite/poly(epsilon-caprolactone) composite scaffolds fabricated by layer manufacturing process
jtitleTissue engineering. Part A
addtitleTissue Eng Part A
date2009-05
risdate2009
volume15
issue5
spage977
epage989
pages977-989
issn1937-3341
eissn1937-335X
abstractThe purpose of this study was to propose a computer-controllable scaffold structure made by a layer manufacturing process (LMP) with addition of nano- or micro-sized particles and to investigate the effects of particle size in vitro. In addition, the superiority of this LMP method over the conventional scaffolds made by salt leaching and gas forming process was investigated through animal study. Using the LMP, we have created a new nano-sized hydroxyapatite/poly(epsilon-caprolactone) composite (n-HPC) scaffold and a micro-sized hydroxyapatite/poly(epsilon-caprolactone) composite (m-HPC) scaffold for bone tissue engineering applications. The scaffold macropores were well interconnected, with a porosity of 73% and a pore size of 500 microm. The compressive modulus of the n-HPC and m-HPC scaffolds was 6.76 and 3.18 MPa, respectively. We compared the cellular responses to the two kinds of scaffolds. Both n-HPC and m-HPC exhibited good in vitro biocompatibility. Attachment and proliferation of mesenchymal stem cells were better on the n-HPC than on the m-HPC scaffold. Moreover, significantly higher alkaline phosphatase activity and calcium content were observed on the n-HPC than on the m-HPC scaffold. In an animal study, the LMP scaffolds enhanced bone formation, owing to their well-interconnected pores. Radiological and histological examinations confirmed that the new bony tissue had grown easily into the entire n-HPC scaffold fabricated by LMP. We suggest that the well-interconnected pores in the LMP scaffolds might encourage cell attachment, proliferation, and migration to stimulate cell functions, thus enhancing bone formation in the LMP scaffolds. This study shows that bioactive and biocompatible n-HPC composite scaffolds prepared using an LMP have potential applications in bone tissue engineering.
copUnited States
pubMary Ann Liebert, Inc
pmid18803480
doi10.1089/ten.tea.2008.0190