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Identification of N-acylhomoserine lactones in mucopurulent respiratory secretions from cystic fibrosis patients

Pseudomonas aeruginosa and species of the Burkholderia cepacia complex are the primary bacterial pathogens contributing to lung disease in patients with cystic fibrosis. Quorum sensing systems using N-acyl homoserine lactone (AHL) signal molecules are involved in the regulation of a number of virule... Full description

Journal Title: FEMS microbiology letters 2005, Vol.244 (2), p.297-304
Main Author: Chambers, Catherine E
Other Authors: Visser, Michelle B , Schwab, Ute , Sokol, Pamela A
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Oxford, UK: Elsevier B.V
ID: ISSN: 0378-1097
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recordid: cdi_proquest_miscellaneous_67511254
title: Identification of N-acylhomoserine lactones in mucopurulent respiratory secretions from cystic fibrosis patients
format: Article
creator:
  • Chambers, Catherine E
  • Visser, Michelle B
  • Schwab, Ute
  • Sokol, Pamela A
subjects:
  • Bacteriology
  • Biological and medical sciences
  • Burkholderia
  • Burkholderia cepacia
  • Burkholderia cepacia - metabolism
  • Cystic fibrosis
  • Cystic Fibrosis - metabolism
  • Cystic Fibrosis - microbiology
  • Epidemiology
  • Fundamental and applied biological sciences. Psychology
  • Homoserine - analysis
  • Homoserine - chemistry
  • Humans
  • L-Homoserine lactone
  • Lactones
  • Lactones - isolation & purification
  • Liquid chromatography
  • Low concentrations
  • Lung diseases
  • Metabolism. Enzymes
  • Microbiology
  • N-Acyl homoserine lactone
  • N-Acylhomoserine lactone
  • N-octanoylhomoserine lactone
  • Pseudomonas aeruginosa
  • Pseudomonas aeruginosa - metabolism
  • Quorum sensing
  • Secretions
  • Signal Transduction
  • Sputum - chemistry
  • Sputum - microbiology
  • Virulence
  • Virulence factors
ispartof: FEMS microbiology letters, 2005, Vol.244 (2), p.297-304
description: Pseudomonas aeruginosa and species of the Burkholderia cepacia complex are the primary bacterial pathogens contributing to lung disease in patients with cystic fibrosis. Quorum sensing systems using N-acyl homoserine lactone (AHL) signal molecules are involved in the regulation of a number of virulence factors in these species. Extracts of mucopurulent respiratory secretions from 13 cystic fibrosis patients infected with P. aeruginosa and/or strains of the B. cepacia complex were fractionated using reverse-phase fast pressure liquid chromatography and analyzed for the presence of AHLs using a traI–luxCDABE-based reporter that responds to AHLs with acyl chains ranging between 4 and 12 carbons. Using this assay system, a broad range of AHLs were detected and identified despite being present at low concentrations in limited sample volumes. N-(3-oxo-dodecanoyl)- l-homoserine lactone, N-(3-oxo-decanoyl)- l-homoserine lactone and N-octanoyl- l-homoserine lactone (OHL) were the AHLs most frequently identified. OHL and N-decanoyl- l-homoserine lactone were detected in nanomolar concentrations compared to picomolar amounts of the 3-oxo-derivatives of the AHLs identified.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0378-1097
fulltext: fulltext
issn:
  • 0378-1097
  • 1574-6968
url: Link


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titleIdentification of N-acylhomoserine lactones in mucopurulent respiratory secretions from cystic fibrosis patients
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creatorcontribChambers, Catherine E ; Visser, Michelle B ; Schwab, Ute ; Sokol, Pamela A
descriptionPseudomonas aeruginosa and species of the Burkholderia cepacia complex are the primary bacterial pathogens contributing to lung disease in patients with cystic fibrosis. Quorum sensing systems using N-acyl homoserine lactone (AHL) signal molecules are involved in the regulation of a number of virulence factors in these species. Extracts of mucopurulent respiratory secretions from 13 cystic fibrosis patients infected with P. aeruginosa and/or strains of the B. cepacia complex were fractionated using reverse-phase fast pressure liquid chromatography and analyzed for the presence of AHLs using a traI–luxCDABE-based reporter that responds to AHLs with acyl chains ranging between 4 and 12 carbons. Using this assay system, a broad range of AHLs were detected and identified despite being present at low concentrations in limited sample volumes. N-(3-oxo-dodecanoyl)- l-homoserine lactone, N-(3-oxo-decanoyl)- l-homoserine lactone and N-octanoyl- l-homoserine lactone (OHL) were the AHLs most frequently identified. OHL and N-decanoyl- l-homoserine lactone were detected in nanomolar concentrations compared to picomolar amounts of the 3-oxo-derivatives of the AHLs identified.
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subjectBacteriology ; Biological and medical sciences ; Burkholderia ; Burkholderia cepacia ; Burkholderia cepacia - metabolism ; Cystic fibrosis ; Cystic Fibrosis - metabolism ; Cystic Fibrosis - microbiology ; Epidemiology ; Fundamental and applied biological sciences. Psychology ; Homoserine - analysis ; Homoserine - chemistry ; Humans ; L-Homoserine lactone ; Lactones ; Lactones - isolation & purification ; Liquid chromatography ; Low concentrations ; Lung diseases ; Metabolism. Enzymes ; Microbiology ; N-Acyl homoserine lactone ; N-Acylhomoserine lactone ; N-octanoylhomoserine lactone ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - metabolism ; Quorum sensing ; Secretions ; Signal Transduction ; Sputum - chemistry ; Sputum - microbiology ; Virulence ; Virulence factors
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descriptionPseudomonas aeruginosa and species of the Burkholderia cepacia complex are the primary bacterial pathogens contributing to lung disease in patients with cystic fibrosis. Quorum sensing systems using N-acyl homoserine lactone (AHL) signal molecules are involved in the regulation of a number of virulence factors in these species. Extracts of mucopurulent respiratory secretions from 13 cystic fibrosis patients infected with P. aeruginosa and/or strains of the B. cepacia complex were fractionated using reverse-phase fast pressure liquid chromatography and analyzed for the presence of AHLs using a traI–luxCDABE-based reporter that responds to AHLs with acyl chains ranging between 4 and 12 carbons. Using this assay system, a broad range of AHLs were detected and identified despite being present at low concentrations in limited sample volumes. N-(3-oxo-dodecanoyl)- l-homoserine lactone, N-(3-oxo-decanoyl)- l-homoserine lactone and N-octanoyl- l-homoserine lactone (OHL) were the AHLs most frequently identified. OHL and N-decanoyl- l-homoserine lactone were detected in nanomolar concentrations compared to picomolar amounts of the 3-oxo-derivatives of the AHLs identified.
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abstractPseudomonas aeruginosa and species of the Burkholderia cepacia complex are the primary bacterial pathogens contributing to lung disease in patients with cystic fibrosis. Quorum sensing systems using N-acyl homoserine lactone (AHL) signal molecules are involved in the regulation of a number of virulence factors in these species. Extracts of mucopurulent respiratory secretions from 13 cystic fibrosis patients infected with P. aeruginosa and/or strains of the B. cepacia complex were fractionated using reverse-phase fast pressure liquid chromatography and analyzed for the presence of AHLs using a traI–luxCDABE-based reporter that responds to AHLs with acyl chains ranging between 4 and 12 carbons. Using this assay system, a broad range of AHLs were detected and identified despite being present at low concentrations in limited sample volumes. N-(3-oxo-dodecanoyl)- l-homoserine lactone, N-(3-oxo-decanoyl)- l-homoserine lactone and N-octanoyl- l-homoserine lactone (OHL) were the AHLs most frequently identified. OHL and N-decanoyl- l-homoserine lactone were detected in nanomolar concentrations compared to picomolar amounts of the 3-oxo-derivatives of the AHLs identified.
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