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(-)-Epigallocatechin gallate enhances the expression of genes related to insulin sensitivity and adipocyte differentiation in 3T3-L1 adipocytes at an early stage of differentiation

Abstract Objective (-)-Epigallocatechin gallate (EGCG) is thought to enhance insulin sensitivity in adipocytes, although doses used in in vitro experiments have been shown to promote apoptosis. To explore the effects of EGCG on insulin sensitivity in adipocytes, the expression of genes related to in... Full description

Journal Title: Nutrition (Burbank Los Angeles County, Calif.), 2009, Vol.25 (10), p.1047-1056
Main Author: Sakurai, Naoko, M.S
Other Authors: Mochizuki, Kazuki, Ph.D , Kameji, Hiroyuki, M.S , Shimada, Masaya, Ph.D , Goda, Toshinao, Ph.D
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
Quelle: Alma/SFX Local Collection
Publisher: New York, NY: Elsevier Inc
ID: ISSN: 0899-9007
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title: (-)-Epigallocatechin gallate enhances the expression of genes related to insulin sensitivity and adipocyte differentiation in 3T3-L1 adipocytes at an early stage of differentiation
format: Article
creator:
  • Sakurai, Naoko, M.S
  • Mochizuki, Kazuki, Ph.D
  • Kameji, Hiroyuki, M.S
  • Shimada, Masaya, Ph.D
  • Goda, Toshinao, Ph.D
subjects:
  • 3T3-L1 Cells
  • Adipocyte
  • Adipocytes - cytology
  • Adipocytes - metabolism
  • Adipogenesis - genetics
  • Animals
  • Biological and medical sciences
  • Catechin
  • Catechin - administration & dosage
  • Catechin - analogs & derivatives
  • Cebps
  • Cell culture
  • Deoxyribonucleic acid
  • Diabetes
  • DNA
  • EGCG
  • Environmental sciences
  • Feeding. Feeding behavior
  • Fundamental and applied biological sciences. Psychology
  • Gastroenterology and Hepatology
  • Gene expression
  • Gene Expression Regulation, Developmental
  • Insulin
  • Insulin - physiology
  • Insulin resistance
  • Insulin sensitivity
  • Lipid Metabolism - genetics
  • Lipids
  • Mice
  • Musculoskeletal system
  • Polymerase chain reaction
  • Pparg
  • Regression Analysis
  • RNA, Messenger - genetics
  • RNA, Messenger - metabolism
  • Rodents
  • Studies
  • Tea - chemistry
  • Vertebrates: anatomy and physiology, studies on body, several organs or systems
ispartof: Nutrition (Burbank, Los Angeles County, Calif.), 2009, Vol.25 (10), p.1047-1056
description: Abstract Objective (-)-Epigallocatechin gallate (EGCG) is thought to enhance insulin sensitivity in adipocytes, although doses used in in vitro experiments have been shown to promote apoptosis. To explore the effects of EGCG on insulin sensitivity in adipocytes, the expression of genes related to insulin sensitivity and adipocyte differentiation in 3T3-L1 adipocytes were measured in response to low doses of EGCG. Methods Increasing concentrations of low-dose EGCG were administered for 8 d to differentiating 3T3 adipocytes, either at days 0–8 (early stage) or at days 8–16 (late stage). Fat accumulation and cell activity were measured by Oil Red O staining and 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay, respectively. The expression of genes related to insulin sensitivity and adipocyte differentiation was measured by real-time reverse transcriptase–polymerase chain reaction. Results Fat accumulation and cell activity in 3T3-L1 cells at the early and late stages were reduced at EGCG concentrations ≥50 μM. However, EGCG doses of 5–10 μM reduced fat accumulation and induced the expression of genes related to insulin sensitivity (including Fabp4, Cd36, Lpl, Pck1, Acox1, Lypla3 , and Ucp2 ) and adipocyte differentiation ( Pparg1, Pparg2, Cebps , and Ppargc1a ). These increases were only seen at the early, and not late, stages of differentiation. Conclusion These data indicate that low doses of EGCG, despite reducing triacylglycerol accumulation, induce the expression of genes related to insulin sensitivity in the early stage of differentiation.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0899-9007
fulltext: fulltext
issn:
  • 0899-9007
  • 1873-1244
url: Link


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title(-)-Epigallocatechin gallate enhances the expression of genes related to insulin sensitivity and adipocyte differentiation in 3T3-L1 adipocytes at an early stage of differentiation
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creatorSakurai, Naoko, M.S ; Mochizuki, Kazuki, Ph.D ; Kameji, Hiroyuki, M.S ; Shimada, Masaya, Ph.D ; Goda, Toshinao, Ph.D
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descriptionAbstract Objective (-)-Epigallocatechin gallate (EGCG) is thought to enhance insulin sensitivity in adipocytes, although doses used in in vitro experiments have been shown to promote apoptosis. To explore the effects of EGCG on insulin sensitivity in adipocytes, the expression of genes related to insulin sensitivity and adipocyte differentiation in 3T3-L1 adipocytes were measured in response to low doses of EGCG. Methods Increasing concentrations of low-dose EGCG were administered for 8 d to differentiating 3T3 adipocytes, either at days 0–8 (early stage) or at days 8–16 (late stage). Fat accumulation and cell activity were measured by Oil Red O staining and 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay, respectively. The expression of genes related to insulin sensitivity and adipocyte differentiation was measured by real-time reverse transcriptase–polymerase chain reaction. Results Fat accumulation and cell activity in 3T3-L1 cells at the early and late stages were reduced at EGCG concentrations ≥50 μM. However, EGCG doses of 5–10 μM reduced fat accumulation and induced the expression of genes related to insulin sensitivity (including Fabp4, Cd36, Lpl, Pck1, Acox1, Lypla3 , and Ucp2 ) and adipocyte differentiation ( Pparg1, Pparg2, Cebps , and Ppargc1a ). These increases were only seen at the early, and not late, stages of differentiation. Conclusion These data indicate that low doses of EGCG, despite reducing triacylglycerol accumulation, induce the expression of genes related to insulin sensitivity in the early stage of differentiation.
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subject3T3-L1 Cells ; Adipocyte ; Adipocytes - cytology ; Adipocytes - metabolism ; Adipogenesis - genetics ; Animals ; Biological and medical sciences ; Catechin ; Catechin - administration & dosage ; Catechin - analogs & derivatives ; Cebps ; Cell culture ; Deoxyribonucleic acid ; Diabetes ; DNA ; EGCG ; Environmental sciences ; Feeding. Feeding behavior ; Fundamental and applied biological sciences. Psychology ; Gastroenterology and Hepatology ; Gene expression ; Gene Expression Regulation, Developmental ; Insulin ; Insulin - physiology ; Insulin resistance ; Insulin sensitivity ; Lipid Metabolism - genetics ; Lipids ; Mice ; Musculoskeletal system ; Polymerase chain reaction ; Pparg ; Regression Analysis ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Rodents ; Studies ; Tea - chemistry ; Vertebrates: anatomy and physiology, studies on body, several organs or systems
ispartofNutrition (Burbank, Los Angeles County, Calif.), 2009, Vol.25 (10), p.1047-1056
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descriptionAbstract Objective (-)-Epigallocatechin gallate (EGCG) is thought to enhance insulin sensitivity in adipocytes, although doses used in in vitro experiments have been shown to promote apoptosis. To explore the effects of EGCG on insulin sensitivity in adipocytes, the expression of genes related to insulin sensitivity and adipocyte differentiation in 3T3-L1 adipocytes were measured in response to low doses of EGCG. Methods Increasing concentrations of low-dose EGCG were administered for 8 d to differentiating 3T3 adipocytes, either at days 0–8 (early stage) or at days 8–16 (late stage). Fat accumulation and cell activity were measured by Oil Red O staining and 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay, respectively. The expression of genes related to insulin sensitivity and adipocyte differentiation was measured by real-time reverse transcriptase–polymerase chain reaction. Results Fat accumulation and cell activity in 3T3-L1 cells at the early and late stages were reduced at EGCG concentrations ≥50 μM. However, EGCG doses of 5–10 μM reduced fat accumulation and induced the expression of genes related to insulin sensitivity (including Fabp4, Cd36, Lpl, Pck1, Acox1, Lypla3 , and Ucp2 ) and adipocyte differentiation ( Pparg1, Pparg2, Cebps , and Ppargc1a ). These increases were only seen at the early, and not late, stages of differentiation. Conclusion These data indicate that low doses of EGCG, despite reducing triacylglycerol accumulation, induce the expression of genes related to insulin sensitivity in the early stage of differentiation.
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31Pparg
32Regression Analysis
33RNA, Messenger - genetics
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title(-)-Epigallocatechin gallate enhances the expression of genes related to insulin sensitivity and adipocyte differentiation in 3T3-L1 adipocytes at an early stage of differentiation
authorSakurai, Naoko, M.S ; Mochizuki, Kazuki, Ph.D ; Kameji, Hiroyuki, M.S ; Shimada, Masaya, Ph.D ; Goda, Toshinao, Ph.D
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03T3-L1 Cells
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jtitleNutrition (Burbank, Los Angeles County, Calif.)
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abstractAbstract Objective (-)-Epigallocatechin gallate (EGCG) is thought to enhance insulin sensitivity in adipocytes, although doses used in in vitro experiments have been shown to promote apoptosis. To explore the effects of EGCG on insulin sensitivity in adipocytes, the expression of genes related to insulin sensitivity and adipocyte differentiation in 3T3-L1 adipocytes were measured in response to low doses of EGCG. Methods Increasing concentrations of low-dose EGCG were administered for 8 d to differentiating 3T3 adipocytes, either at days 0–8 (early stage) or at days 8–16 (late stage). Fat accumulation and cell activity were measured by Oil Red O staining and 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan assay, respectively. The expression of genes related to insulin sensitivity and adipocyte differentiation was measured by real-time reverse transcriptase–polymerase chain reaction. Results Fat accumulation and cell activity in 3T3-L1 cells at the early and late stages were reduced at EGCG concentrations ≥50 μM. However, EGCG doses of 5–10 μM reduced fat accumulation and induced the expression of genes related to insulin sensitivity (including Fabp4, Cd36, Lpl, Pck1, Acox1, Lypla3 , and Ucp2 ) and adipocyte differentiation ( Pparg1, Pparg2, Cebps , and Ppargc1a ). These increases were only seen at the early, and not late, stages of differentiation. Conclusion These data indicate that low doses of EGCG, despite reducing triacylglycerol accumulation, induce the expression of genes related to insulin sensitivity in the early stage of differentiation.
copNew York, NY
pubElsevier Inc
pmid19535224
doi10.1016/j.nut.2009.02.012