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Reduction of viral loads by multigenic DNA priming and adenovirus boosting in the SIVmac-macaque model

In this study, we investigated the ability of a multigenic SIV DNA prime/replication-defective adenovirus serotype 5 (rAd/SIV) boost regimen to induce SIV-specific immune responses and protection against intrarectal challenge with SIVmac251 in rhesus macaques. Four rhesus macaques were immunized int... Full description

Journal Title: Vaccine 2006, Vol.24 (11), p.1811-1820
Main Author: Suh, You S
Other Authors: Park, Ki S , Sauermann, Ulrike , Franz, Monika , Norley, Stephen , Wilfingseder, Doris , Stoiber, Heribert , Fagrouch, Zahra , Heeney, Jonathan , Hunsmann, Gerhard , Stahl-Hennig, Christiane , Sung, Young C
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
HIV
SIV
Quelle: Alma/SFX Local Collection
Publisher: Oxford: Elsevier Ltd
ID: ISSN: 0264-410X
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title: Reduction of viral loads by multigenic DNA priming and adenovirus boosting in the SIVmac-macaque model
format: Article
creator:
  • Suh, You S
  • Park, Ki S
  • Sauermann, Ulrike
  • Franz, Monika
  • Norley, Stephen
  • Wilfingseder, Doris
  • Stoiber, Heribert
  • Fagrouch, Zahra
  • Heeney, Jonathan
  • Hunsmann, Gerhard
  • Stahl-Hennig, Christiane
  • Sung, Young C
subjects:
  • Acquired immune deficiency syndrome
  • Adenoviridae - genetics
  • Adenoviridae - immunology
  • Adenovirus
  • Adjuvants, Immunologic
  • AIDS
  • AIDS vaccines
  • Animals
  • Antibodies
  • Antibodies, Viral - blood
  • Applied microbiology
  • Biological and medical sciences
  • CD4 Lymphocyte Count
  • Cytokines
  • Deoxyribonucleic acid
  • DNA
  • Fundamental and applied biological sciences. Psychology
  • Gene expression
  • Genetic Vectors
  • Health aspects
  • HIV
  • Human immunodeficiency virus
  • IL-12
  • Immunization, Secondary
  • Infections
  • Injections, Intramuscular
  • Interferon-gamma - biosynthesis
  • Interleukin-12 - genetics
  • Interleukin-12 - pharmacology
  • Lymphocytes
  • Macaca mulatta
  • Microbiology
  • Miscellaneous
  • Multigenic vaccine
  • Neutralization Tests
  • Plasmids
  • Primates
  • Prime-boost
  • Proteins
  • SAIDS Vaccines - administration & dosage
  • SAIDS Vaccines - immunology
  • Simian Acquired Immunodeficiency Syndrome - immunology
  • Simian Acquired Immunodeficiency Syndrome - prevention & control
  • Simian Acquired Immunodeficiency Syndrome - virology
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus - immunology
  • Simian Immunodeficiency Virus - isolation & purification
  • SIV
  • T cells
  • T-Lymphocytes - immunology
  • Vaccination
  • Vaccines
  • Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
  • Vaccines, DNA - administration & dosage
  • Vaccines, DNA - immunology
  • Viral antibodies
  • Viral Load
  • Viral Nonstructural Proteins - genetics
  • Viral Nonstructural Proteins - immunology
  • Viral Structural Proteins - genetics
  • Viral Structural Proteins - immunology
  • Viremia
  • Virology
ispartof: Vaccine, 2006, Vol.24 (11), p.1811-1820
description: In this study, we investigated the ability of a multigenic SIV DNA prime/replication-defective adenovirus serotype 5 (rAd/SIV) boost regimen to induce SIV-specific immune responses and protection against intrarectal challenge with SIVmac251 in rhesus macaques. Four rhesus macaques were immunized intramuscularly three times at 8-week intervals with SIV DNA vaccine and boosted once with rAd/SIV vaccine Four control macaques received the same amount of mock plasmid DNA and mock adenovirus vector. While the SIV DNA vaccine included plasmids expressing a mutated human IL-12 gene (IL-12N222L) as well as SIVmac239 structural and regulatory genes, the rAd/SIV vaccine contained rAd vectors expressing SIVmac239 genes only. Immunization with SIV DNA vaccine alone induced SIV-specific IFN-γ ELISPOT responses in only two of four vaccinated macaques, whereas all animals developed SIV-specific T-cell responses and Env- and Tat-specific antibody responses following the rAd/SIV vaccine boost. Upon intrarectal challenge with pathogenic SIVmac251, strong anamnestic Env-specific binding and neutralizing antibody responses were detected in the vaccinated macaques. Overall, the immunized macaques had lower peak and set-point viral loads than control macaques, suggesting that the induced immune responses play a role in the control of viremia. In addition, the loss of CD4 + T cells was delayed in the vaccinated macaques after challenge. These results indicate that the multigenic DNA prime-adenovirus boost immunization may be a promising approach in developing an effective AIDS vaccine.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0264-410X
fulltext: fulltext
issn:
  • 0264-410X
  • 1873-2518
url: Link


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titleReduction of viral loads by multigenic DNA priming and adenovirus boosting in the SIVmac-macaque model
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creatorSuh, You S ; Park, Ki S ; Sauermann, Ulrike ; Franz, Monika ; Norley, Stephen ; Wilfingseder, Doris ; Stoiber, Heribert ; Fagrouch, Zahra ; Heeney, Jonathan ; Hunsmann, Gerhard ; Stahl-Hennig, Christiane ; Sung, Young C
creatorcontribSuh, You S ; Park, Ki S ; Sauermann, Ulrike ; Franz, Monika ; Norley, Stephen ; Wilfingseder, Doris ; Stoiber, Heribert ; Fagrouch, Zahra ; Heeney, Jonathan ; Hunsmann, Gerhard ; Stahl-Hennig, Christiane ; Sung, Young C
descriptionIn this study, we investigated the ability of a multigenic SIV DNA prime/replication-defective adenovirus serotype 5 (rAd/SIV) boost regimen to induce SIV-specific immune responses and protection against intrarectal challenge with SIVmac251 in rhesus macaques. Four rhesus macaques were immunized intramuscularly three times at 8-week intervals with SIV DNA vaccine and boosted once with rAd/SIV vaccine Four control macaques received the same amount of mock plasmid DNA and mock adenovirus vector. While the SIV DNA vaccine included plasmids expressing a mutated human IL-12 gene (IL-12N222L) as well as SIVmac239 structural and regulatory genes, the rAd/SIV vaccine contained rAd vectors expressing SIVmac239 genes only. Immunization with SIV DNA vaccine alone induced SIV-specific IFN-γ ELISPOT responses in only two of four vaccinated macaques, whereas all animals developed SIV-specific T-cell responses and Env- and Tat-specific antibody responses following the rAd/SIV vaccine boost. Upon intrarectal challenge with pathogenic SIVmac251, strong anamnestic Env-specific binding and neutralizing antibody responses were detected in the vaccinated macaques. Overall, the immunized macaques had lower peak and set-point viral loads than control macaques, suggesting that the induced immune responses play a role in the control of viremia. In addition, the loss of CD4 + T cells was delayed in the vaccinated macaques after challenge. These results indicate that the multigenic DNA prime-adenovirus boost immunization may be a promising approach in developing an effective AIDS vaccine.
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0ISSN: 0264-410X
1EISSN: 1873-2518
2DOI: 10.1016/j.vaccine.2005.10.026
3PMID: 16274888
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languageeng
publisherOxford: Elsevier Ltd
subjectAcquired immune deficiency syndrome ; Adenoviridae - genetics ; Adenoviridae - immunology ; Adenovirus ; Adjuvants, Immunologic ; AIDS ; AIDS vaccines ; Animals ; Antibodies ; Antibodies, Viral - blood ; Applied microbiology ; Biological and medical sciences ; CD4 Lymphocyte Count ; Cytokines ; Deoxyribonucleic acid ; DNA ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Genetic Vectors ; Health aspects ; HIV ; Human immunodeficiency virus ; IL-12 ; Immunization, Secondary ; Infections ; Injections, Intramuscular ; Interferon-gamma - biosynthesis ; Interleukin-12 - genetics ; Interleukin-12 - pharmacology ; Lymphocytes ; Macaca mulatta ; Microbiology ; Miscellaneous ; Multigenic vaccine ; Neutralization Tests ; Plasmids ; Primates ; Prime-boost ; Proteins ; SAIDS Vaccines - administration & dosage ; SAIDS Vaccines - immunology ; Simian Acquired Immunodeficiency Syndrome - immunology ; Simian Acquired Immunodeficiency Syndrome - prevention & control ; Simian Acquired Immunodeficiency Syndrome - virology ; Simian immunodeficiency virus ; Simian Immunodeficiency Virus - immunology ; Simian Immunodeficiency Virus - isolation & purification ; SIV ; T cells ; T-Lymphocytes - immunology ; Vaccination ; Vaccines ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Vaccines, DNA - administration & dosage ; Vaccines, DNA - immunology ; Viral antibodies ; Viral Load ; Viral Nonstructural Proteins - genetics ; Viral Nonstructural Proteins - immunology ; Viral Structural Proteins - genetics ; Viral Structural Proteins - immunology ; Viremia ; Virology
ispartofVaccine, 2006, Vol.24 (11), p.1811-1820
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0Suh, You S
1Park, Ki S
2Sauermann, Ulrike
3Franz, Monika
4Norley, Stephen
5Wilfingseder, Doris
6Stoiber, Heribert
7Fagrouch, Zahra
8Heeney, Jonathan
9Hunsmann, Gerhard
10Stahl-Hennig, Christiane
11Sung, Young C
title
0Reduction of viral loads by multigenic DNA priming and adenovirus boosting in the SIVmac-macaque model
1Vaccine
addtitleVaccine
descriptionIn this study, we investigated the ability of a multigenic SIV DNA prime/replication-defective adenovirus serotype 5 (rAd/SIV) boost regimen to induce SIV-specific immune responses and protection against intrarectal challenge with SIVmac251 in rhesus macaques. Four rhesus macaques were immunized intramuscularly three times at 8-week intervals with SIV DNA vaccine and boosted once with rAd/SIV vaccine Four control macaques received the same amount of mock plasmid DNA and mock adenovirus vector. While the SIV DNA vaccine included plasmids expressing a mutated human IL-12 gene (IL-12N222L) as well as SIVmac239 structural and regulatory genes, the rAd/SIV vaccine contained rAd vectors expressing SIVmac239 genes only. Immunization with SIV DNA vaccine alone induced SIV-specific IFN-γ ELISPOT responses in only two of four vaccinated macaques, whereas all animals developed SIV-specific T-cell responses and Env- and Tat-specific antibody responses following the rAd/SIV vaccine boost. Upon intrarectal challenge with pathogenic SIVmac251, strong anamnestic Env-specific binding and neutralizing antibody responses were detected in the vaccinated macaques. Overall, the immunized macaques had lower peak and set-point viral loads than control macaques, suggesting that the induced immune responses play a role in the control of viremia. In addition, the loss of CD4 + T cells was delayed in the vaccinated macaques after challenge. These results indicate that the multigenic DNA prime-adenovirus boost immunization may be a promising approach in developing an effective AIDS vaccine.
subject
0Acquired immune deficiency syndrome
1Adenoviridae - genetics
2Adenoviridae - immunology
3Adenovirus
4Adjuvants, Immunologic
5AIDS
6AIDS vaccines
7Animals
8Antibodies
9Antibodies, Viral - blood
10Applied microbiology
11Biological and medical sciences
12CD4 Lymphocyte Count
13Cytokines
14Deoxyribonucleic acid
15DNA
16Fundamental and applied biological sciences. Psychology
17Gene expression
18Genetic Vectors
19Health aspects
20HIV
21Human immunodeficiency virus
22IL-12
23Immunization, Secondary
24Infections
25Injections, Intramuscular
26Interferon-gamma - biosynthesis
27Interleukin-12 - genetics
28Interleukin-12 - pharmacology
29Lymphocytes
30Macaca mulatta
31Microbiology
32Miscellaneous
33Multigenic vaccine
34Neutralization Tests
35Plasmids
36Primates
37Prime-boost
38Proteins
39SAIDS Vaccines - administration & dosage
40SAIDS Vaccines - immunology
41Simian Acquired Immunodeficiency Syndrome - immunology
42Simian Acquired Immunodeficiency Syndrome - prevention & control
43Simian Acquired Immunodeficiency Syndrome - virology
44Simian immunodeficiency virus
45Simian Immunodeficiency Virus - immunology
46Simian Immunodeficiency Virus - isolation & purification
47SIV
48T cells
49T-Lymphocytes - immunology
50Vaccination
51Vaccines
52Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
53Vaccines, DNA - administration & dosage
54Vaccines, DNA - immunology
55Viral antibodies
56Viral Load
57Viral Nonstructural Proteins - genetics
58Viral Nonstructural Proteins - immunology
59Viral Structural Proteins - genetics
60Viral Structural Proteins - immunology
61Viremia
62Virology
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7Fagrouch, Zahra
8Heeney, Jonathan
9Hunsmann, Gerhard
10Stahl-Hennig, Christiane
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titleReduction of viral loads by multigenic DNA priming and adenovirus boosting in the SIVmac-macaque model
authorSuh, You S ; Park, Ki S ; Sauermann, Ulrike ; Franz, Monika ; Norley, Stephen ; Wilfingseder, Doris ; Stoiber, Heribert ; Fagrouch, Zahra ; Heeney, Jonathan ; Hunsmann, Gerhard ; Stahl-Hennig, Christiane ; Sung, Young C
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0Acquired immune deficiency syndrome
1Adenoviridae - genetics
2Adenoviridae - immunology
3Adenovirus
4Adjuvants, Immunologic
5AIDS
6AIDS vaccines
7Animals
8Antibodies
9Antibodies, Viral - blood
10Applied microbiology
11Biological and medical sciences
12CD4 Lymphocyte Count
13Cytokines
14Deoxyribonucleic acid
15DNA
16Fundamental and applied biological sciences. Psychology
17Gene expression
18Genetic Vectors
19Health aspects
20HIV
21Human immunodeficiency virus
22IL-12
23Immunization, Secondary
24Infections
25Injections, Intramuscular
26Interferon-gamma - biosynthesis
27Interleukin-12 - genetics
28Interleukin-12 - pharmacology
29Lymphocytes
30Macaca mulatta
31Microbiology
32Miscellaneous
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34Neutralization Tests
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36Primates
37Prime-boost
38Proteins
39SAIDS Vaccines - administration & dosage
40SAIDS Vaccines - immunology
41Simian Acquired Immunodeficiency Syndrome - immunology
42Simian Acquired Immunodeficiency Syndrome - prevention & control
43Simian Acquired Immunodeficiency Syndrome - virology
44Simian immunodeficiency virus
45Simian Immunodeficiency Virus - immunology
46Simian Immunodeficiency Virus - isolation & purification
47SIV
48T cells
49T-Lymphocytes - immunology
50Vaccination
51Vaccines
52Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
53Vaccines, DNA - administration & dosage
54Vaccines, DNA - immunology
55Viral antibodies
56Viral Load
57Viral Nonstructural Proteins - genetics
58Viral Nonstructural Proteins - immunology
59Viral Structural Proteins - genetics
60Viral Structural Proteins - immunology
61Viremia
62Virology
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abstractIn this study, we investigated the ability of a multigenic SIV DNA prime/replication-defective adenovirus serotype 5 (rAd/SIV) boost regimen to induce SIV-specific immune responses and protection against intrarectal challenge with SIVmac251 in rhesus macaques. Four rhesus macaques were immunized intramuscularly three times at 8-week intervals with SIV DNA vaccine and boosted once with rAd/SIV vaccine Four control macaques received the same amount of mock plasmid DNA and mock adenovirus vector. While the SIV DNA vaccine included plasmids expressing a mutated human IL-12 gene (IL-12N222L) as well as SIVmac239 structural and regulatory genes, the rAd/SIV vaccine contained rAd vectors expressing SIVmac239 genes only. Immunization with SIV DNA vaccine alone induced SIV-specific IFN-γ ELISPOT responses in only two of four vaccinated macaques, whereas all animals developed SIV-specific T-cell responses and Env- and Tat-specific antibody responses following the rAd/SIV vaccine boost. Upon intrarectal challenge with pathogenic SIVmac251, strong anamnestic Env-specific binding and neutralizing antibody responses were detected in the vaccinated macaques. Overall, the immunized macaques had lower peak and set-point viral loads than control macaques, suggesting that the induced immune responses play a role in the control of viremia. In addition, the loss of CD4 + T cells was delayed in the vaccinated macaques after challenge. These results indicate that the multigenic DNA prime-adenovirus boost immunization may be a promising approach in developing an effective AIDS vaccine.
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pubElsevier Ltd
pmid16274888
doi10.1016/j.vaccine.2005.10.026