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The Listeria monocytogenes prfAP2 promoter is regulated by sigma B in a growth phase dependent manner

Listeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters ( prfAP1, prfAP2, and P plcA). The prfAP2 promoter was previously proposed to be sigma B (σ B)-dependent. This hypothesis was tested by creating prfA promoter- gus transcriptional fusions in both... Full description

Journal Title: FEMS microbiology letters 2005, Vol.245 (2), p.329-336
Main Author: Schwab, Ute
Other Authors: Bowen, Barbara , Nadon, Celine , Wiedmann, Martin , Boor, Kathryn J
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Oxford, UK: Elsevier B.V
ID: ISSN: 0378-1097
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title: The Listeria monocytogenes prfAP2 promoter is regulated by sigma B in a growth phase dependent manner
format: Article
creator:
  • Schwab, Ute
  • Bowen, Barbara
  • Nadon, Celine
  • Wiedmann, Martin
  • Boor, Kathryn J
subjects:
  • Artificial Gene Fusion
  • Bacterial Proteins - genetics
  • Bacterial Proteins - physiology
  • Bacteriology
  • Biological and medical sciences
  • Fluorescent Antibody Technique
  • Fundamental and applied biological sciences. Psychology
  • Gene Deletion
  • Gene Expression Regulation, Bacterial
  • Genes, Reporter
  • Genetics
  • Glucuronidase - analysis
  • Glucuronidase - genetics
  • gus Reporter gene
  • Immunofluorescence
  • Listeria
  • Listeria monocytogenes
  • Listeria monocytogenes - genetics
  • Listeria monocytogenes - growth & development
  • Microbiology
  • Pathogens
  • Peptide Termination Factors
  • prfA
  • Promoter Regions, Genetic - physiology
  • RNA, Bacterial - analysis
  • RNA, Messenger - analysis
  • Sigma B
  • Sigma Factor - genetics
  • Sigma Factor - physiology
  • Stationary phase
  • Trans-Activators - genetics
  • Transcription
ispartof: FEMS microbiology letters, 2005, Vol.245 (2), p.329-336
description: Listeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters ( prfAP1, prfAP2, and P plcA). The prfAP2 promoter was previously proposed to be sigma B (σ B)-dependent. This hypothesis was tested by creating prfA promoter- gus transcriptional fusions in both L. monocytogenes wild-type (wt) and Δ sigB backgrounds and then measuring (i) β-glucuronidase (GUS) activities; (ii) gus mRNA transcript levels; and (iii) the presence or absence of GUS in cells by immunofluorescence staining. prfAP2-directed expression increased as the wt L. monocytogenes strain entered stationary phase, whereas prfAP2-directed expression was greatly reduced in the Δ sigB strain, confirming both growth phase- and σ B-dependent transcription of prfAP2. We conclude that prfAP2 is directly regulated by σ B.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0378-1097
fulltext: fulltext
issn:
  • 0378-1097
  • 1574-6968
url: Link


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titleThe Listeria monocytogenes prfAP2 promoter is regulated by sigma B in a growth phase dependent manner
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creatorSchwab, Ute ; Bowen, Barbara ; Nadon, Celine ; Wiedmann, Martin ; Boor, Kathryn J
creatorcontribSchwab, Ute ; Bowen, Barbara ; Nadon, Celine ; Wiedmann, Martin ; Boor, Kathryn J
descriptionListeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters ( prfAP1, prfAP2, and P plcA). The prfAP2 promoter was previously proposed to be sigma B (σ B)-dependent. This hypothesis was tested by creating prfA promoter- gus transcriptional fusions in both L. monocytogenes wild-type (wt) and Δ sigB backgrounds and then measuring (i) β-glucuronidase (GUS) activities; (ii) gus mRNA transcript levels; and (iii) the presence or absence of GUS in cells by immunofluorescence staining. prfAP2-directed expression increased as the wt L. monocytogenes strain entered stationary phase, whereas prfAP2-directed expression was greatly reduced in the Δ sigB strain, confirming both growth phase- and σ B-dependent transcription of prfAP2. We conclude that prfAP2 is directly regulated by σ B.
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3PMID: 15837390
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languageeng
publisherOxford, UK: Elsevier B.V
subjectArtificial Gene Fusion ; Bacterial Proteins - genetics ; Bacterial Proteins - physiology ; Bacteriology ; Biological and medical sciences ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Gene Expression Regulation, Bacterial ; Genes, Reporter ; Genetics ; Glucuronidase - analysis ; Glucuronidase - genetics ; gus Reporter gene ; Immunofluorescence ; Listeria ; Listeria monocytogenes ; Listeria monocytogenes - genetics ; Listeria monocytogenes - growth & development ; Microbiology ; Pathogens ; Peptide Termination Factors ; prfA ; Promoter Regions, Genetic - physiology ; RNA, Bacterial - analysis ; RNA, Messenger - analysis ; Sigma B ; Sigma Factor - genetics ; Sigma Factor - physiology ; Stationary phase ; Trans-Activators - genetics ; Transcription
ispartofFEMS microbiology letters, 2005, Vol.245 (2), p.329-336
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descriptionListeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters ( prfAP1, prfAP2, and P plcA). The prfAP2 promoter was previously proposed to be sigma B (σ B)-dependent. This hypothesis was tested by creating prfA promoter- gus transcriptional fusions in both L. monocytogenes wild-type (wt) and Δ sigB backgrounds and then measuring (i) β-glucuronidase (GUS) activities; (ii) gus mRNA transcript levels; and (iii) the presence or absence of GUS in cells by immunofluorescence staining. prfAP2-directed expression increased as the wt L. monocytogenes strain entered stationary phase, whereas prfAP2-directed expression was greatly reduced in the Δ sigB strain, confirming both growth phase- and σ B-dependent transcription of prfAP2. We conclude that prfAP2 is directly regulated by σ B.
subject
0Artificial Gene Fusion
1Bacterial Proteins - genetics
2Bacterial Proteins - physiology
3Bacteriology
4Biological and medical sciences
5Fluorescent Antibody Technique
6Fundamental and applied biological sciences. Psychology
7Gene Deletion
8Gene Expression Regulation, Bacterial
9Genes, Reporter
10Genetics
11Glucuronidase - analysis
12Glucuronidase - genetics
13gus Reporter gene
14Immunofluorescence
15Listeria
16Listeria monocytogenes
17Listeria monocytogenes - genetics
18Listeria monocytogenes - growth & development
19Microbiology
20Pathogens
21Peptide Termination Factors
22prfA
23Promoter Regions, Genetic - physiology
24RNA, Bacterial - analysis
25RNA, Messenger - analysis
26Sigma B
27Sigma Factor - genetics
28Sigma Factor - physiology
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30Trans-Activators - genetics
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authorSchwab, Ute ; Bowen, Barbara ; Nadon, Celine ; Wiedmann, Martin ; Boor, Kathryn J
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1Bacterial Proteins - genetics
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3Bacteriology
4Biological and medical sciences
5Fluorescent Antibody Technique
6Fundamental and applied biological sciences. Psychology
7Gene Deletion
8Gene Expression Regulation, Bacterial
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13gus Reporter gene
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20Pathogens
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abstractListeria monocytogenes prfA, encoding positive regulatory factor A, is transcribed from three promoters ( prfAP1, prfAP2, and P plcA). The prfAP2 promoter was previously proposed to be sigma B (σ B)-dependent. This hypothesis was tested by creating prfA promoter- gus transcriptional fusions in both L. monocytogenes wild-type (wt) and Δ sigB backgrounds and then measuring (i) β-glucuronidase (GUS) activities; (ii) gus mRNA transcript levels; and (iii) the presence or absence of GUS in cells by immunofluorescence staining. prfAP2-directed expression increased as the wt L. monocytogenes strain entered stationary phase, whereas prfAP2-directed expression was greatly reduced in the Δ sigB strain, confirming both growth phase- and σ B-dependent transcription of prfAP2. We conclude that prfAP2 is directly regulated by σ B.
copOxford, UK
pubElsevier B.V
pmid15837390
doi10.1016/j.femsle.2005.03.025
tpages8