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Molecular cloning and expression of an alpha-amylase inhibitor from rye with potential for controlling insect pests

Alpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. In this work, we report the cloning and expression of a gene encoding fo... Full description

Journal Title: The Protein Journal 2005, Vol.24 (2), p.113-123
Main Author: Dias, Simoni C
Other Authors: Franco, Octávio L , Magalhães, Cláudio P , de Oliveira-Neto, Osmundo B , Laumann, Raúl A , Figueira, Edson L Z , Melo, Francislete R , Grossi-De-Sá, Maria F
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
Quelle: Alma/SFX Local Collection
Publisher: Netherlands
ID: ISSN: 1572-3887
Link: https://www.ncbi.nlm.nih.gov/pubmed/16003953
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title: Molecular cloning and expression of an alpha-amylase inhibitor from rye with potential for controlling insect pests
format: Article
creator:
  • Dias, Simoni C
  • Franco, Octávio L
  • Magalhães, Cláudio P
  • de Oliveira-Neto, Osmundo B
  • Laumann, Raúl A
  • Figueira, Edson L Z
  • Melo, Francislete R
  • Grossi-De-Sá, Maria F
subjects:
  • alpha-Amylases - antagonists & inhibitors
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA
  • DNA Primers
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme Inhibitors - chemistry
  • Escherichia coli - genetics
  • Insecta
  • Models, Molecular
  • Molecular Sequence Data
  • Pest Control, Biological
  • Secale - chemistry
  • Sequence Homology, Amino Acid
ispartof: The Protein Journal, 2005, Vol.24 (2), p.113-123
description: Alpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. In this work, we report the cloning and expression of a gene encoding for a new alpha-amylase inhibitor (BIII) from rye (Secale cereale) seeds. The BIII gene contains 354 nucleotides that encode for 118 amino acids sequence. A 313 bp fragment of the gene was expressed in Escherichia coli and resulted in a functional inhibitor that reduced the activity of alpha-amylases of larvae of the coleopteran pests Acanthoscelides obtectus, Zabrotess subfasciatus and Anthonomus grandis. In contrast, the inhibitor did not inhibit the activity of porcine pancreatic alpha-amylase. Although the amino acid sequence of BIII showed high identity with those of bifunctional inhibitors, the recombinant protein was unable to inhibit trypsin-like serine proteinases. The effects of recombinant BIII were evaluated in vivo against A. grandis. When first instar larvae were reared on an artificial diet containing four different concentrations of BIII, a reduction in larval weight and a mortality of 83% were observed at the highest concentration.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 1572-3887
fulltext: fulltext
issn:
  • 1572-3887
  • 1875-8355
  • 1573-4943
url: Link


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titleMolecular cloning and expression of an alpha-amylase inhibitor from rye with potential for controlling insect pests
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creatorDias, Simoni C ; Franco, Octávio L ; Magalhães, Cláudio P ; de Oliveira-Neto, Osmundo B ; Laumann, Raúl A ; Figueira, Edson L Z ; Melo, Francislete R ; Grossi-De-Sá, Maria F
creatorcontribDias, Simoni C ; Franco, Octávio L ; Magalhães, Cláudio P ; de Oliveira-Neto, Osmundo B ; Laumann, Raúl A ; Figueira, Edson L Z ; Melo, Francislete R ; Grossi-De-Sá, Maria F
descriptionAlpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. In this work, we report the cloning and expression of a gene encoding for a new alpha-amylase inhibitor (BIII) from rye (Secale cereale) seeds. The BIII gene contains 354 nucleotides that encode for 118 amino acids sequence. A 313 bp fragment of the gene was expressed in Escherichia coli and resulted in a functional inhibitor that reduced the activity of alpha-amylases of larvae of the coleopteran pests Acanthoscelides obtectus, Zabrotess subfasciatus and Anthonomus grandis. In contrast, the inhibitor did not inhibit the activity of porcine pancreatic alpha-amylase. Although the amino acid sequence of BIII showed high identity with those of bifunctional inhibitors, the recombinant protein was unable to inhibit trypsin-like serine proteinases. The effects of recombinant BIII were evaluated in vivo against A. grandis. When first instar larvae were reared on an artificial diet containing four different concentrations of BIII, a reduction in larval weight and a mortality of 83% were observed at the highest concentration.
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publisherNetherlands
subjectalpha-Amylases - antagonists & inhibitors ; Amino Acid Sequence ; Animals ; Base Sequence ; Cloning, Molecular ; DNA ; DNA Primers ; Electrophoresis, Polyacrylamide Gel ; Enzyme Inhibitors - chemistry ; Escherichia coli - genetics ; Insecta ; Models, Molecular ; Molecular Sequence Data ; Pest Control, Biological ; Secale - chemistry ; Sequence Homology, Amino Acid
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descriptionAlpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. In this work, we report the cloning and expression of a gene encoding for a new alpha-amylase inhibitor (BIII) from rye (Secale cereale) seeds. The BIII gene contains 354 nucleotides that encode for 118 amino acids sequence. A 313 bp fragment of the gene was expressed in Escherichia coli and resulted in a functional inhibitor that reduced the activity of alpha-amylases of larvae of the coleopteran pests Acanthoscelides obtectus, Zabrotess subfasciatus and Anthonomus grandis. In contrast, the inhibitor did not inhibit the activity of porcine pancreatic alpha-amylase. Although the amino acid sequence of BIII showed high identity with those of bifunctional inhibitors, the recombinant protein was unable to inhibit trypsin-like serine proteinases. The effects of recombinant BIII were evaluated in vivo against A. grandis. When first instar larvae were reared on an artificial diet containing four different concentrations of BIII, a reduction in larval weight and a mortality of 83% were observed at the highest concentration.
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abstractAlpha-amylase inhibitors have important roles in plant defense mechanisms, particularly against insects, and several of these inhibitors have been expressed in different crops to increase their resistance to particular insects. In this work, we report the cloning and expression of a gene encoding for a new alpha-amylase inhibitor (BIII) from rye (Secale cereale) seeds. The BIII gene contains 354 nucleotides that encode for 118 amino acids sequence. A 313 bp fragment of the gene was expressed in Escherichia coli and resulted in a functional inhibitor that reduced the activity of alpha-amylases of larvae of the coleopteran pests Acanthoscelides obtectus, Zabrotess subfasciatus and Anthonomus grandis. In contrast, the inhibitor did not inhibit the activity of porcine pancreatic alpha-amylase. Although the amino acid sequence of BIII showed high identity with those of bifunctional inhibitors, the recombinant protein was unable to inhibit trypsin-like serine proteinases. The effects of recombinant BIII were evaluated in vivo against A. grandis. When first instar larvae were reared on an artificial diet containing four different concentrations of BIII, a reduction in larval weight and a mortality of 83% were observed at the highest concentration.
copNetherlands
pmid16003953
doi10.1007/s10930-004-1518-4