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Characterization of the bacterial community associated with the surface and mucus layer of whiting (Merlangius merlangus)

Abstract The bacterial community inhabiting the mucus layer and surface of whiting was examined to determine whether the bacteria present are a reflection of the surrounding water or an indigenous bacterial flora is present. The outer mucus, mouth mucus and gut of four whiting harvested from a site... Full description

Journal Title: FEMS microbiology ecology 2007-10-01, Vol.62 (1), p.90-97
Main Author: Smith, Cindy J
Other Authors: Danilowicz, Bret S , Meijer, Wim G
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Oxford, UK: Blackwell Publishing Ltd
ID: ISSN: 0168-6496
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recordid: cdi_proquest_miscellaneous_68333410
title: Characterization of the bacterial community associated with the surface and mucus layer of whiting (Merlangius merlangus)
format: Article
creator:
  • Smith, Cindy J
  • Danilowicz, Bret S
  • Meijer, Wim G
subjects:
  • 16S rRNA gene
  • Agnatha. Pisces
  • Animal, plant and microbial ecology
  • Animals
  • Bacteria
  • Bacteria - classification
  • Bacteria - genetics
  • Bacteria - isolation & purification
  • Biodiversity
  • Biological and medical sciences
  • Cloning
  • Cluster Analysis
  • Communities
  • DNA Fingerprinting
  • DNA, Bacterial - chemistry
  • DNA, Bacterial - genetics
  • DNA, Ribosomal - chemistry
  • DNA, Ribosomal - genetics
  • fish mucus layer bacterial community
  • Flora
  • Fundamental and applied biological sciences. Psychology
  • Gadiformes - microbiology
  • Gene polymorphism
  • Intestines - microbiology
  • Ireland
  • Merlangius merlangus
  • Microbial ecology
  • Molecular Sequence Data
  • Mouth - microbiology
  • Mucus
  • Mucus - microbiology
  • Photobacterium
  • Photobacterium iliopiscarium
  • Polymorphism
  • Polymorphism, Restriction Fragment Length
  • Restriction fragment length polymorphism
  • RNA, Ribosomal, 16S - genetics
  • rRNA 16S
  • Seawater - microbiology
  • Sequence Analysis, DNA
  • Skin - microbiology
  • terminal restriction fragment length polymorphism
  • Various environments (extraatmospheric space, air, water)
  • Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
  • Water circulation
  • Water column
ispartof: FEMS microbiology ecology, 2007-10-01, Vol.62 (1), p.90-97
description: Abstract The bacterial community inhabiting the mucus layer and surface of whiting was examined to determine whether the bacteria present are a reflection of the surrounding water or an indigenous bacterial flora is present. The outer mucus, mouth mucus and gut of four whiting harvested from a site in the Irish Sea and the surrounding water were examined by terminal restriction fragment length polymorphism (tRFLP) analysis of the 16S rRNA gene and clone library construction. The water community was the most diverse, with only a small number of shared water-mucus phylotypes present. The bacterial flora associated with the outer mucus layer were more diverse than that of the mouth mucus and gut. All three mucus layers were characterized by the presence of a dominant phylotype, identified as clone wom-1, highly similar to Photobacterium iliopiscarium. In addition to other Photobacterium phylotypes, members of the CFB and Clostridia groups were also detected. Subsequently, whiting from 11 different sites along the east and south coast of Ireland were compared by tRFLP analysis. Strikingly, the mucus layer of whiting at all sites was characterized by the presence and dominance of a TRF corresponding to the clone wom-1 which was virtually absent from the water column.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0168-6496
fulltext: fulltext
issn:
  • 0168-6496
  • 1574-6941
url: Link


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creatorcontribSmith, Cindy J ; Danilowicz, Bret S ; Meijer, Wim G
descriptionAbstract The bacterial community inhabiting the mucus layer and surface of whiting was examined to determine whether the bacteria present are a reflection of the surrounding water or an indigenous bacterial flora is present. The outer mucus, mouth mucus and gut of four whiting harvested from a site in the Irish Sea and the surrounding water were examined by terminal restriction fragment length polymorphism (tRFLP) analysis of the 16S rRNA gene and clone library construction. The water community was the most diverse, with only a small number of shared water-mucus phylotypes present. The bacterial flora associated with the outer mucus layer were more diverse than that of the mouth mucus and gut. All three mucus layers were characterized by the presence of a dominant phylotype, identified as clone wom-1, highly similar to Photobacterium iliopiscarium. In addition to other Photobacterium phylotypes, members of the CFB and Clostridia groups were also detected. Subsequently, whiting from 11 different sites along the east and south coast of Ireland were compared by tRFLP analysis. Strikingly, the mucus layer of whiting at all sites was characterized by the presence and dominance of a TRF corresponding to the clone wom-1 which was virtually absent from the water column.
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languageeng
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subject16S rRNA gene ; Agnatha. Pisces ; Animal, plant and microbial ecology ; Animals ; Bacteria ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Biodiversity ; Biological and medical sciences ; Cloning ; Cluster Analysis ; Communities ; DNA Fingerprinting ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; fish mucus layer bacterial community ; Flora ; Fundamental and applied biological sciences. Psychology ; Gadiformes - microbiology ; Gene polymorphism ; Intestines - microbiology ; Ireland ; Merlangius merlangus ; Microbial ecology ; Molecular Sequence Data ; Mouth - microbiology ; Mucus ; Mucus - microbiology ; Photobacterium ; Photobacterium iliopiscarium ; Polymorphism ; Polymorphism, Restriction Fragment Length ; Restriction fragment length polymorphism ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Seawater - microbiology ; Sequence Analysis, DNA ; Skin - microbiology ; terminal restriction fragment length polymorphism ; Various environments (extraatmospheric space, air, water) ; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution ; Water circulation ; Water column
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descriptionAbstract The bacterial community inhabiting the mucus layer and surface of whiting was examined to determine whether the bacteria present are a reflection of the surrounding water or an indigenous bacterial flora is present. The outer mucus, mouth mucus and gut of four whiting harvested from a site in the Irish Sea and the surrounding water were examined by terminal restriction fragment length polymorphism (tRFLP) analysis of the 16S rRNA gene and clone library construction. The water community was the most diverse, with only a small number of shared water-mucus phylotypes present. The bacterial flora associated with the outer mucus layer were more diverse than that of the mouth mucus and gut. All three mucus layers were characterized by the presence of a dominant phylotype, identified as clone wom-1, highly similar to Photobacterium iliopiscarium. In addition to other Photobacterium phylotypes, members of the CFB and Clostridia groups were also detected. Subsequently, whiting from 11 different sites along the east and south coast of Ireland were compared by tRFLP analysis. Strikingly, the mucus layer of whiting at all sites was characterized by the presence and dominance of a TRF corresponding to the clone wom-1 which was virtually absent from the water column.
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016S rRNA gene
1Agnatha. Pisces
2Animal, plant and microbial ecology
3Animals
4Bacteria
5Bacteria - classification
6Bacteria - genetics
7Bacteria - isolation & purification
8Biodiversity
9Biological and medical sciences
10Cloning
11Cluster Analysis
12Communities
13DNA Fingerprinting
14DNA, Bacterial - chemistry
15DNA, Bacterial - genetics
16DNA, Ribosomal - chemistry
17DNA, Ribosomal - genetics
18fish mucus layer bacterial community
19Flora
20Fundamental and applied biological sciences. Psychology
21Gadiformes - microbiology
22Gene polymorphism
23Intestines - microbiology
24Ireland
25Merlangius merlangus
26Microbial ecology
27Molecular Sequence Data
28Mouth - microbiology
29Mucus
30Mucus - microbiology
31Photobacterium
32Photobacterium iliopiscarium
33Polymorphism
34Polymorphism, Restriction Fragment Length
35Restriction fragment length polymorphism
36RNA, Ribosomal, 16S - genetics
37rRNA 16S
38Seawater - microbiology
39Sequence Analysis, DNA
40Skin - microbiology
41terminal restriction fragment length polymorphism
42Various environments (extraatmospheric space, air, water)
43Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
44Water circulation
45Water column
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016S rRNA gene
1Agnatha. Pisces
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4Bacteria
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6Bacteria - genetics
7Bacteria - isolation & purification
8Biodiversity
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16DNA, Ribosomal - chemistry
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atitleCharacterization of the bacterial community associated with the surface and mucus layer of whiting (Merlangius merlangus)
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date2007-10-01
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volume62
issue1
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issn0168-6496
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notesEditor: Julian Marchesi
abstractAbstract The bacterial community inhabiting the mucus layer and surface of whiting was examined to determine whether the bacteria present are a reflection of the surrounding water or an indigenous bacterial flora is present. The outer mucus, mouth mucus and gut of four whiting harvested from a site in the Irish Sea and the surrounding water were examined by terminal restriction fragment length polymorphism (tRFLP) analysis of the 16S rRNA gene and clone library construction. The water community was the most diverse, with only a small number of shared water-mucus phylotypes present. The bacterial flora associated with the outer mucus layer were more diverse than that of the mouth mucus and gut. All three mucus layers were characterized by the presence of a dominant phylotype, identified as clone wom-1, highly similar to Photobacterium iliopiscarium. In addition to other Photobacterium phylotypes, members of the CFB and Clostridia groups were also detected. Subsequently, whiting from 11 different sites along the east and south coast of Ireland were compared by tRFLP analysis. Strikingly, the mucus layer of whiting at all sites was characterized by the presence and dominance of a TRF corresponding to the clone wom-1 which was virtually absent from the water column.
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