schliessen

Filtern

 

Bibliotheken

Pharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin

(-)-Epigallocatechin gallate (EGCG), the main physiologically active polyphenol of green tea, is associated with antitumor and antimutagenic activities. The goal of this study was to determine the stability and pharmacokinetic parameters of pure EGCG administered topically to human and mouse skin. E... Full description

Journal Title: Cancer chemotherapy and pharmacology 1999, Vol.43 (4), p.331-335
Main Author: DVORAKOVA, K
Other Authors: DORR, R. T , VALCIC, S , TIMMERMANN, B , ALBERTS, D. S
Format: Electronic Article Electronic Article
Language: English
Subjects:
Tea
Publisher: Berlin: Springer
ID: ISSN: 0344-5704
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_proquest_miscellaneous_69614086
title: Pharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin
format: Article
creator:
  • DVORAKOVA, K
  • DORR, R. T
  • VALCIC, S
  • TIMMERMANN, B
  • ALBERTS, D. S
subjects:
  • Administration, Topical
  • Animals
  • Anticarcinogenic Agents - administration & dosage
  • Anticarcinogenic Agents - pharmacokinetics
  • Biological and medical sciences
  • Carcinogenesis, carcinogens and anticarcinogens
  • Catechin - administration & dosage
  • Catechin - analogs & derivatives
  • Catechin - pharmacokinetics
  • Chemical agents
  • Female
  • General pharmacology
  • Humans
  • In Vitro Techniques
  • Medical sciences
  • Mice
  • Mice, Inbred BALB C
  • Permeability
  • Pharmacognosy. Homeopathy. Health food
  • Pharmacology. Drug treatments
  • Skin - metabolism
  • Skin Absorption
  • Tea
  • Time Factors
  • Tumors
ispartof: Cancer chemotherapy and pharmacology, 1999, Vol.43 (4), p.331-335
description: (-)-Epigallocatechin gallate (EGCG), the main physiologically active polyphenol of green tea, is associated with antitumor and antimutagenic activities. The goal of this study was to determine the stability and pharmacokinetic parameters of pure EGCG administered topically to human and mouse skin. EGCG was investigated by measuring drug levels of a 10% ointment formulation stored under different conditions over a period of 6 months. To determine pharmacokinetic parameters of EGCG following topical application. EGCG was applied as 10% EGCG in hydrophilic ointment USP to full-thickness mouse or human skin in vitro. The transdermal and intradermal. Penetration of EGCG was measured by reverse phase HPLC assays at different time-points. The stability of EGCG in hydrophilic ointment USP was dependent on time, temperature and the degree of oxidation. For example, 10% EGCG was lost after 2 days at 37 degrees C, but the same formulation supplemented with 0.1% butylated hydroxytoluene (BHT) had significantly longer stability with > or =90% EGCG remaining after 130 days at 37 degrees C. Topical application of EGCG in hydrophilic ointment USP to human or mouse skin resulted in substantial intradermal uptake of up to 1-20% of the applied dose. However, transdermal penetration was observed only in mouse skin. The present study showed that topical application of EGCG in hydrophilic ointment USP achieved high concentrations in skin but negligible systemic availability. The drug was susceptible to oxidation, but if supplemented with BHT, the hydrophilic ointment formulation could potentially be used in clinical trials of skin cancer prevention.
language: eng
source:
identifier: ISSN: 0344-5704
fulltext: no_fulltext
issn:
  • 0344-5704
  • 1432-0843
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.087317
LOCALfalse
PrimoNMBib
record
control
sourceidproquest_cross
recordidTN_cdi_proquest_miscellaneous_69614086
sourceformatXML
sourcesystemPC
sourcerecordid69614086
originalsourceidFETCH-LOGICAL-c309t-f18853f4ac531192d088249aa6fb62ced694240002f8fb1ecfd28e9925583cca0
addsrcrecordideNp1kE1vEzEQhi0EoqFw5Ip8QJy6MP7YjX1EUQlIleAA59XEOyaGXW-wvZX67-s0kfiQOM3lmfedeRh7KeCtAFi_ywDSAEALFtQjthJayQaMVo_ZCpTWTbsGfcGe5fyjUloo9ZRdHDeFNe2KpS97TBO6-WeIVILLfPa87Il_T0SRF0I-UAq3WMItXfHr7WZ7xXd3D0iZD8HhyNO8FDru4TCFGHJJlZ4jD5FP85KJYxz4fpkw8lxrnrMnHsdML87zkn37cP1187G5-bz9tHl_0zgFtjReGNMqr9G1SggrBzBGaovY-V0nHQ2d1VLXn6Q3fifI-UEasla2rVHOIVyyN6fcQ5p_LZRLP4XsaBwxUj2r72wnNJiugs0JdGnOOZHvDylMmO56Af1RVf-X5Mq_Ogcvu4mGP-iT1Qq8PgOYqyCfMLqQf3OdWUt5zFH_9LpQHtRVg2H8T_s9puOT6Q
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid69614086
display
typearticle
titlePharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin
creatorDVORAKOVA, K ; DORR, R. T ; VALCIC, S ; TIMMERMANN, B ; ALBERTS, D. S
creatorcontribDVORAKOVA, K ; DORR, R. T ; VALCIC, S ; TIMMERMANN, B ; ALBERTS, D. S
description(-)-Epigallocatechin gallate (EGCG), the main physiologically active polyphenol of green tea, is associated with antitumor and antimutagenic activities. The goal of this study was to determine the stability and pharmacokinetic parameters of pure EGCG administered topically to human and mouse skin. EGCG was investigated by measuring drug levels of a 10% ointment formulation stored under different conditions over a period of 6 months. To determine pharmacokinetic parameters of EGCG following topical application. EGCG was applied as 10% EGCG in hydrophilic ointment USP to full-thickness mouse or human skin in vitro. The transdermal and intradermal. Penetration of EGCG was measured by reverse phase HPLC assays at different time-points. The stability of EGCG in hydrophilic ointment USP was dependent on time, temperature and the degree of oxidation. For example, 10% EGCG was lost after 2 days at 37 degrees C, but the same formulation supplemented with 0.1% butylated hydroxytoluene (BHT) had significantly longer stability with > or =90% EGCG remaining after 130 days at 37 degrees C. Topical application of EGCG in hydrophilic ointment USP to human or mouse skin resulted in substantial intradermal uptake of up to 1-20% of the applied dose. However, transdermal penetration was observed only in mouse skin. The present study showed that topical application of EGCG in hydrophilic ointment USP achieved high concentrations in skin but negligible systemic availability. The drug was susceptible to oxidation, but if supplemented with BHT, the hydrophilic ointment formulation could potentially be used in clinical trials of skin cancer prevention.
identifier
0ISSN: 0344-5704
1EISSN: 1432-0843
2DOI: 10.1007/s002800050903
3PMID: 10071985
4CODEN: CCPHDZ
languageeng
publisherBerlin: Springer
subjectAdministration, Topical ; Animals ; Anticarcinogenic Agents - administration & dosage ; Anticarcinogenic Agents - pharmacokinetics ; Biological and medical sciences ; Carcinogenesis, carcinogens and anticarcinogens ; Catechin - administration & dosage ; Catechin - analogs & derivatives ; Catechin - pharmacokinetics ; Chemical agents ; Female ; General pharmacology ; Humans ; In Vitro Techniques ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Permeability ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Skin - metabolism ; Skin Absorption ; Tea ; Time Factors ; Tumors
ispartofCancer chemotherapy and pharmacology, 1999, Vol.43 (4), p.331-335
rights1999 INIST-CNRS
lds50peer_reviewed
citedbyFETCH-LOGICAL-c309t-f18853f4ac531192d088249aa6fb62ced694240002f8fb1ecfd28e9925583cca0
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink
0$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1687223$$DView record in Pascal Francis
1$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/10071985$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0DVORAKOVA, K
1DORR, R. T
2VALCIC, S
3TIMMERMANN, B
4ALBERTS, D. S
title
0Pharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin
1Cancer chemotherapy and pharmacology
addtitleCancer Chemother Pharmacol
description(-)-Epigallocatechin gallate (EGCG), the main physiologically active polyphenol of green tea, is associated with antitumor and antimutagenic activities. The goal of this study was to determine the stability and pharmacokinetic parameters of pure EGCG administered topically to human and mouse skin. EGCG was investigated by measuring drug levels of a 10% ointment formulation stored under different conditions over a period of 6 months. To determine pharmacokinetic parameters of EGCG following topical application. EGCG was applied as 10% EGCG in hydrophilic ointment USP to full-thickness mouse or human skin in vitro. The transdermal and intradermal. Penetration of EGCG was measured by reverse phase HPLC assays at different time-points. The stability of EGCG in hydrophilic ointment USP was dependent on time, temperature and the degree of oxidation. For example, 10% EGCG was lost after 2 days at 37 degrees C, but the same formulation supplemented with 0.1% butylated hydroxytoluene (BHT) had significantly longer stability with > or =90% EGCG remaining after 130 days at 37 degrees C. Topical application of EGCG in hydrophilic ointment USP to human or mouse skin resulted in substantial intradermal uptake of up to 1-20% of the applied dose. However, transdermal penetration was observed only in mouse skin. The present study showed that topical application of EGCG in hydrophilic ointment USP achieved high concentrations in skin but negligible systemic availability. The drug was susceptible to oxidation, but if supplemented with BHT, the hydrophilic ointment formulation could potentially be used in clinical trials of skin cancer prevention.
subject
0Administration, Topical
1Animals
2Anticarcinogenic Agents - administration & dosage
3Anticarcinogenic Agents - pharmacokinetics
4Biological and medical sciences
5Carcinogenesis, carcinogens and anticarcinogens
6Catechin - administration & dosage
7Catechin - analogs & derivatives
8Catechin - pharmacokinetics
9Chemical agents
10Female
11General pharmacology
12Humans
13In Vitro Techniques
14Medical sciences
15Mice
16Mice, Inbred BALB C
17Permeability
18Pharmacognosy. Homeopathy. Health food
19Pharmacology. Drug treatments
20Skin - metabolism
21Skin Absorption
22Tea
23Time Factors
24Tumors
issn
00344-5704
11432-0843
fulltextfalse
rsrctypearticle
creationdate1999
recordtypearticle
recordideNp1kE1vEzEQhi0EoqFw5Ip8QJy6MP7YjX1EUQlIleAA59XEOyaGXW-wvZX67-s0kfiQOM3lmfedeRh7KeCtAFi_ywDSAEALFtQjthJayQaMVo_ZCpTWTbsGfcGe5fyjUloo9ZRdHDeFNe2KpS97TBO6-WeIVILLfPa87Il_T0SRF0I-UAq3WMItXfHr7WZ7xXd3D0iZD8HhyNO8FDru4TCFGHJJlZ4jD5FP85KJYxz4fpkw8lxrnrMnHsdML87zkn37cP1187G5-bz9tHl_0zgFtjReGNMqr9G1SggrBzBGaovY-V0nHQ2d1VLXn6Q3fifI-UEasla2rVHOIVyyN6fcQ5p_LZRLP4XsaBwxUj2r72wnNJiugs0JdGnOOZHvDylMmO56Af1RVf-X5Mq_Ogcvu4mGP-iT1Qq8PgOYqyCfMLqQf3OdWUt5zFH_9LpQHtRVg2H8T_s9puOT6Q
startdate1999
enddate1999
creator
0DVORAKOVA, K
1DORR, R. T
2VALCIC, S
3TIMMERMANN, B
4ALBERTS, D. S
generalSpringer
scope
0IQODW
1CGR
2CUY
3CVF
4ECM
5EIF
6NPM
7AAYXX
8CITATION
97X8
sort
creationdate1999
titlePharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin
authorDVORAKOVA, K ; DORR, R. T ; VALCIC, S ; TIMMERMANN, B ; ALBERTS, D. S
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-c309t-f18853f4ac531192d088249aa6fb62ced694240002f8fb1ecfd28e9925583cca0
rsrctypearticles
prefilterarticles
languageeng
creationdate1999
topic
0Administration, Topical
1Animals
2Anticarcinogenic Agents - administration & dosage
3Anticarcinogenic Agents - pharmacokinetics
4Biological and medical sciences
5Carcinogenesis, carcinogens and anticarcinogens
6Catechin - administration & dosage
7Catechin - analogs & derivatives
8Catechin - pharmacokinetics
9Chemical agents
10Female
11General pharmacology
12Humans
13In Vitro Techniques
14Medical sciences
15Mice
16Mice, Inbred BALB C
17Permeability
18Pharmacognosy. Homeopathy. Health food
19Pharmacology. Drug treatments
20Skin - metabolism
21Skin Absorption
22Tea
23Time Factors
24Tumors
toplevelpeer_reviewed
creatorcontrib
0DVORAKOVA, K
1DORR, R. T
2VALCIC, S
3TIMMERMANN, B
4ALBERTS, D. S
collection
0Pascal-Francis
1Medline
2MEDLINE
3MEDLINE (Ovid)
4MEDLINE
5MEDLINE
6PubMed
7CrossRef
8MEDLINE - Academic
jtitleCancer chemotherapy and pharmacology
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0DVORAKOVA, K
1DORR, R. T
2VALCIC, S
3TIMMERMANN, B
4ALBERTS, D. S
formatjournal
genrearticle
ristypeJOUR
atitlePharmacokinetics of the green tea derivative, EGCG, by the topical route of administration in mouse and human skin
jtitleCancer chemotherapy and pharmacology
addtitleCancer Chemother Pharmacol
date1999
risdate1999
volume43
issue4
spage331
epage335
pages331-335
issn0344-5704
eissn1432-0843
codenCCPHDZ
abstract(-)-Epigallocatechin gallate (EGCG), the main physiologically active polyphenol of green tea, is associated with antitumor and antimutagenic activities. The goal of this study was to determine the stability and pharmacokinetic parameters of pure EGCG administered topically to human and mouse skin. EGCG was investigated by measuring drug levels of a 10% ointment formulation stored under different conditions over a period of 6 months. To determine pharmacokinetic parameters of EGCG following topical application. EGCG was applied as 10% EGCG in hydrophilic ointment USP to full-thickness mouse or human skin in vitro. The transdermal and intradermal. Penetration of EGCG was measured by reverse phase HPLC assays at different time-points. The stability of EGCG in hydrophilic ointment USP was dependent on time, temperature and the degree of oxidation. For example, 10% EGCG was lost after 2 days at 37 degrees C, but the same formulation supplemented with 0.1% butylated hydroxytoluene (BHT) had significantly longer stability with > or =90% EGCG remaining after 130 days at 37 degrees C. Topical application of EGCG in hydrophilic ointment USP to human or mouse skin resulted in substantial intradermal uptake of up to 1-20% of the applied dose. However, transdermal penetration was observed only in mouse skin. The present study showed that topical application of EGCG in hydrophilic ointment USP achieved high concentrations in skin but negligible systemic availability. The drug was susceptible to oxidation, but if supplemented with BHT, the hydrophilic ointment formulation could potentially be used in clinical trials of skin cancer prevention.
copBerlin
pubSpringer
pmid10071985
doi10.1007/s002800050903