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Characterisation of caveolins from cartilage: Expression of caveolin-1, -2 and -3 in chondrocytes and in alginate cell culture of the rat tibia

This study was performed to determine if rat articular chondrocytes express caveolin, the structural protein of caveolae, and to determine differences in the distribution of the caveolin subtypes 1, 2 and 3 in knee joints of newborn and adult rats. All three subtypes of caveolin were detected in adu... Full description

Journal Title: Histochemistry and cell biology 1999, Vol.112 (1), p.41-49
Main Author: Schwab, W
Other Authors: Galbiati, F , Volonte, D , Hempel, U , Wenzel, K W , Funk, R H , Lisanti, M P , Kasper, M
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Germany: Springer Nature B.V
ID: ISSN: 0948-6143
Link: https://www.ncbi.nlm.nih.gov/pubmed/10461811
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title: Characterisation of caveolins from cartilage: Expression of caveolin-1, -2 and -3 in chondrocytes and in alginate cell culture of the rat tibia
format: Article
creator:
  • Schwab, W
  • Galbiati, F
  • Volonte, D
  • Hempel, U
  • Wenzel, K W
  • Funk, R H
  • Lisanti, M P
  • Kasper, M
subjects:
  • Alginates
  • Alginic acid
  • Animals
  • Animals, Newborn
  • Cartilage
  • Cartilage (articular)
  • Cartilage, Articular - metabolism
  • Caveolae
  • Caveolin
  • Caveolin 1
  • Caveolin 2
  • Caveolin 3
  • Caveolins
  • Cell culture
  • Cells, Cultured
  • Chondrocytes
  • Chondrocytes - metabolism
  • Chondrogenesis
  • DNA Primers - chemistry
  • Female
  • Fluorescent Antibody Technique, Indirect
  • Glucuronic Acid
  • Hexuronic Acids
  • Immunoenzyme Techniques
  • Isoforms
  • Knee Joint
  • Male
  • Membrane Proteins - biosynthesis
  • Membrane Proteins - genetics
  • mRNA
  • Rats
  • Rats, Wistar
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger - metabolism
  • Rodents
  • Tibia
  • Tibia - cytology
  • Tibia - metabolism
  • Tissue culture
ispartof: Histochemistry and cell biology, 1999, Vol.112 (1), p.41-49
description: This study was performed to determine if rat articular chondrocytes express caveolin, the structural protein of caveolae, and to determine differences in the distribution of the caveolin subtypes 1, 2 and 3 in knee joints of newborn and adult rats. All three subtypes of caveolin were detected in adult cartilage by immunocytochemical staining. In newborn rats, only caveolin-1 was found in the hyaline cartilage. Caveolin-1, -2 and -3 messenger RNA and protein were also detected in chondrocyte cell cultures. Ultrastructural investigations of cell culture and cartilage tissue revealed the presence of caveolae at the plasma membrane of chondrocytes. These findings represent the first report on the different expression of caveolin isoforms, in particular the expression of the muscle cell-specific caveolin-3 in chondrocytes. There is evidence that caveolin-2 and -3 are upregulated during growth and development of articular cartilage, suggesting a role for caveolins in chondrocyte differentiation.
language: eng
source:
identifier: ISSN: 0948-6143
fulltext: no_fulltext
issn:
  • 0948-6143
  • 1432-119X
url: Link


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titleCharacterisation of caveolins from cartilage: Expression of caveolin-1, -2 and -3 in chondrocytes and in alginate cell culture of the rat tibia
creatorSchwab, W ; Galbiati, F ; Volonte, D ; Hempel, U ; Wenzel, K W ; Funk, R H ; Lisanti, M P ; Kasper, M
creatorcontribSchwab, W ; Galbiati, F ; Volonte, D ; Hempel, U ; Wenzel, K W ; Funk, R H ; Lisanti, M P ; Kasper, M
descriptionThis study was performed to determine if rat articular chondrocytes express caveolin, the structural protein of caveolae, and to determine differences in the distribution of the caveolin subtypes 1, 2 and 3 in knee joints of newborn and adult rats. All three subtypes of caveolin were detected in adult cartilage by immunocytochemical staining. In newborn rats, only caveolin-1 was found in the hyaline cartilage. Caveolin-1, -2 and -3 messenger RNA and protein were also detected in chondrocyte cell cultures. Ultrastructural investigations of cell culture and cartilage tissue revealed the presence of caveolae at the plasma membrane of chondrocytes. These findings represent the first report on the different expression of caveolin isoforms, in particular the expression of the muscle cell-specific caveolin-3 in chondrocytes. There is evidence that caveolin-2 and -3 are upregulated during growth and development of articular cartilage, suggesting a role for caveolins in chondrocyte differentiation.
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subjectAlginates ; Alginic acid ; Animals ; Animals, Newborn ; Cartilage ; Cartilage (articular) ; Cartilage, Articular - metabolism ; Caveolae ; Caveolin ; Caveolin 1 ; Caveolin 2 ; Caveolin 3 ; Caveolins ; Cell culture ; Cells, Cultured ; Chondrocytes ; Chondrocytes - metabolism ; Chondrogenesis ; DNA Primers - chemistry ; Female ; Fluorescent Antibody Technique, Indirect ; Glucuronic Acid ; Hexuronic Acids ; Immunoenzyme Techniques ; Isoforms ; Knee Joint ; Male ; Membrane Proteins - biosynthesis ; Membrane Proteins - genetics ; mRNA ; Rats ; Rats, Wistar ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - metabolism ; Rodents ; Tibia ; Tibia - cytology ; Tibia - metabolism ; Tissue culture
ispartofHistochemistry and cell biology, 1999, Vol.112 (1), p.41-49
rightsSpringer-Verlag Berlin Heidelberg 1999.
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1Histochemistry and cell biology
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descriptionThis study was performed to determine if rat articular chondrocytes express caveolin, the structural protein of caveolae, and to determine differences in the distribution of the caveolin subtypes 1, 2 and 3 in knee joints of newborn and adult rats. All three subtypes of caveolin were detected in adult cartilage by immunocytochemical staining. In newborn rats, only caveolin-1 was found in the hyaline cartilage. Caveolin-1, -2 and -3 messenger RNA and protein were also detected in chondrocyte cell cultures. Ultrastructural investigations of cell culture and cartilage tissue revealed the presence of caveolae at the plasma membrane of chondrocytes. These findings represent the first report on the different expression of caveolin isoforms, in particular the expression of the muscle cell-specific caveolin-3 in chondrocytes. There is evidence that caveolin-2 and -3 are upregulated during growth and development of articular cartilage, suggesting a role for caveolins in chondrocyte differentiation.
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0Alginates
1Alginic acid
2Animals
3Animals, Newborn
4Cartilage
5Cartilage (articular)
6Cartilage, Articular - metabolism
7Caveolae
8Caveolin
9Caveolin 1
10Caveolin 2
11Caveolin 3
12Caveolins
13Cell culture
14Cells, Cultured
15Chondrocytes
16Chondrocytes - metabolism
17Chondrogenesis
18DNA Primers - chemistry
19Female
20Fluorescent Antibody Technique, Indirect
21Glucuronic Acid
22Hexuronic Acids
23Immunoenzyme Techniques
24Isoforms
25Knee Joint
26Male
27Membrane Proteins - biosynthesis
28Membrane Proteins - genetics
29mRNA
30Rats
31Rats, Wistar
32Reverse Transcriptase Polymerase Chain Reaction
33RNA, Messenger - metabolism
34Rodents
35Tibia
36Tibia - cytology
37Tibia - metabolism
38Tissue culture
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11432-119X
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titleCharacterisation of caveolins from cartilage: Expression of caveolin-1, -2 and -3 in chondrocytes and in alginate cell culture of the rat tibia
authorSchwab, W ; Galbiati, F ; Volonte, D ; Hempel, U ; Wenzel, K W ; Funk, R H ; Lisanti, M P ; Kasper, M
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0Alginates
1Alginic acid
2Animals
3Animals, Newborn
4Cartilage
5Cartilage (articular)
6Cartilage, Articular - metabolism
7Caveolae
8Caveolin
9Caveolin 1
10Caveolin 2
11Caveolin 3
12Caveolins
13Cell culture
14Cells, Cultured
15Chondrocytes
16Chondrocytes - metabolism
17Chondrogenesis
18DNA Primers - chemistry
19Female
20Fluorescent Antibody Technique, Indirect
21Glucuronic Acid
22Hexuronic Acids
23Immunoenzyme Techniques
24Isoforms
25Knee Joint
26Male
27Membrane Proteins - biosynthesis
28Membrane Proteins - genetics
29mRNA
30Rats
31Rats, Wistar
32Reverse Transcriptase Polymerase Chain Reaction
33RNA, Messenger - metabolism
34Rodents
35Tibia
36Tibia - cytology
37Tibia - metabolism
38Tissue culture
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1Galbiati, F
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atitleCharacterisation of caveolins from cartilage: Expression of caveolin-1, -2 and -3 in chondrocytes and in alginate cell culture of the rat tibia
jtitleHistochemistry and cell biology
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date1999-07
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abstractThis study was performed to determine if rat articular chondrocytes express caveolin, the structural protein of caveolae, and to determine differences in the distribution of the caveolin subtypes 1, 2 and 3 in knee joints of newborn and adult rats. All three subtypes of caveolin were detected in adult cartilage by immunocytochemical staining. In newborn rats, only caveolin-1 was found in the hyaline cartilage. Caveolin-1, -2 and -3 messenger RNA and protein were also detected in chondrocyte cell cultures. Ultrastructural investigations of cell culture and cartilage tissue revealed the presence of caveolae at the plasma membrane of chondrocytes. These findings represent the first report on the different expression of caveolin isoforms, in particular the expression of the muscle cell-specific caveolin-3 in chondrocytes. There is evidence that caveolin-2 and -3 are upregulated during growth and development of articular cartilage, suggesting a role for caveolins in chondrocyte differentiation.
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pubSpringer Nature B.V
pmid10461811
doi10.1007/s004180050390