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Non-destructive mechanical characterisation of UVA/riboflavin crosslinked collagen hydrogels

Aims:To establish a non-destructive method of characterising the mechanical properties of collagen hydrogels to model corneal tissue and to examine the effect of photochemical crosslinking on their mechanical properties.Methods:Collagen hydrogels were manufactured, submerged in 0.1% riboflavin solut... Full description

Journal Title: British journal of ophthalmology 2008-02, Vol.92 (2), p.268-271
Main Author: Ahearne, M
Other Authors: Yang, Y , Then, K Y , Liu, K-K
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: BMA House, Tavistock Square, London, WC1H 9JR: BMJ Publishing Group Ltd
ID: ISSN: 0007-1161
Zum Text:
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recordid: cdi_proquest_miscellaneous_70242866
title: Non-destructive mechanical characterisation of UVA/riboflavin crosslinked collagen hydrogels
format: Article
creator:
  • Ahearne, M
  • Yang, Y
  • Then, K Y
  • Liu, K-K
subjects:
  • agriculture
  • Biological and medical sciences
  • Biological models
  • Biomechanical Phenomena
  • Care and treatment
  • Chemical properties
  • Collagen
  • Collagen - chemistry
  • Collagen - drug effects
  • Collagen - radiation effects
  • Cornea - drug effects
  • Cornea - physiology
  • Cornea - radiation effects
  • Cross-Linking Reagents - chemistry
  • Fibroblasts
  • Genetic aspects
  • Humans
  • Hydrogels
  • Hydrogels - chemistry
  • Hydrogels - radiation effects
  • industry
  • Keratoconus
  • macromolecular substances
  • Mechanical properties
  • Medical sciences
  • Miscellaneous
  • Models, Biological
  • Ophthalmology
  • Physiological aspects
  • Research
  • Riboflavin - pharmacology
  • sense organs
  • technology
  • Time Factors
  • Ultraviolet Rays
  • Vitamin B
  • Vitamin B2
ispartof: British journal of ophthalmology, 2008-02, Vol.92 (2), p.268-271
description: Aims:To establish a non-destructive method of characterising the mechanical properties of collagen hydrogels to model corneal tissue and to examine the effect of photochemical crosslinking on their mechanical properties.Methods:Collagen hydrogels were manufactured, submerged in 0.1% riboflavin solution and crosslinked using two UVA tube bulbs with an intensity of between 2.8 and 3.2 mW/cm2. The hydrogels were clamped around their outer edge and deformed using a sphere. The deformation was measured in situ using a long-working-distance microscope connected to a CCD camera, and the deformation displacement was used with a theoretical model to calculate the Young modulus of the hydrogels. Collagen hydrogels seeded with human corneal fibroblasts were used to examine cell viability after UVA irradiation.Results:There was an increase in Young modulus of the collagen hydrogels after UVA/riboflavin treatment that was dependent on the exposure time. UVA irradiation without riboflavin showed decreased mechanical integrity and strength. Cell viability was reduced with increased UVA exposure time.Conclusion:The non-destructive technique demonstrated a new methodology comparable with strip extensiometry for cornea or corneal model specimens but with more convenient features. This approach could be used as an initial step in developing new crosslinking treatments for patients with keratoconus.
language: eng
source:
identifier: ISSN: 0007-1161
fulltext: no_fulltext
issn:
  • 0007-1161
  • 1468-2079
url: Link


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titleNon-destructive mechanical characterisation of UVA/riboflavin crosslinked collagen hydrogels
creatorAhearne, M ; Yang, Y ; Then, K Y ; Liu, K-K
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descriptionAims:To establish a non-destructive method of characterising the mechanical properties of collagen hydrogels to model corneal tissue and to examine the effect of photochemical crosslinking on their mechanical properties.Methods:Collagen hydrogels were manufactured, submerged in 0.1% riboflavin solution and crosslinked using two UVA tube bulbs with an intensity of between 2.8 and 3.2 mW/cm2. The hydrogels were clamped around their outer edge and deformed using a sphere. The deformation was measured in situ using a long-working-distance microscope connected to a CCD camera, and the deformation displacement was used with a theoretical model to calculate the Young modulus of the hydrogels. Collagen hydrogels seeded with human corneal fibroblasts were used to examine cell viability after UVA irradiation.Results:There was an increase in Young modulus of the collagen hydrogels after UVA/riboflavin treatment that was dependent on the exposure time. UVA irradiation without riboflavin showed decreased mechanical integrity and strength. Cell viability was reduced with increased UVA exposure time.Conclusion:The non-destructive technique demonstrated a new methodology comparable with strip extensiometry for cornea or corneal model specimens but with more convenient features. This approach could be used as an initial step in developing new crosslinking treatments for patients with keratoconus.
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subjectagriculture ; Biological and medical sciences ; Biological models ; Biomechanical Phenomena ; Care and treatment ; Chemical properties ; Collagen ; Collagen - chemistry ; Collagen - drug effects ; Collagen - radiation effects ; Cornea - drug effects ; Cornea - physiology ; Cornea - radiation effects ; Cross-Linking Reagents - chemistry ; Fibroblasts ; Genetic aspects ; Humans ; Hydrogels ; Hydrogels - chemistry ; Hydrogels - radiation effects ; industry ; Keratoconus ; macromolecular substances ; Mechanical properties ; Medical sciences ; Miscellaneous ; Models, Biological ; Ophthalmology ; Physiological aspects ; Research ; Riboflavin - pharmacology ; sense organs ; technology ; Time Factors ; Ultraviolet Rays ; Vitamin B ; Vitamin B2
ispartofBritish journal of ophthalmology, 2008-02, Vol.92 (2), p.268-271
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descriptionAims:To establish a non-destructive method of characterising the mechanical properties of collagen hydrogels to model corneal tissue and to examine the effect of photochemical crosslinking on their mechanical properties.Methods:Collagen hydrogels were manufactured, submerged in 0.1% riboflavin solution and crosslinked using two UVA tube bulbs with an intensity of between 2.8 and 3.2 mW/cm2. The hydrogels were clamped around their outer edge and deformed using a sphere. The deformation was measured in situ using a long-working-distance microscope connected to a CCD camera, and the deformation displacement was used with a theoretical model to calculate the Young modulus of the hydrogels. Collagen hydrogels seeded with human corneal fibroblasts were used to examine cell viability after UVA irradiation.Results:There was an increase in Young modulus of the collagen hydrogels after UVA/riboflavin treatment that was dependent on the exposure time. UVA irradiation without riboflavin showed decreased mechanical integrity and strength. Cell viability was reduced with increased UVA exposure time.Conclusion:The non-destructive technique demonstrated a new methodology comparable with strip extensiometry for cornea or corneal model specimens but with more convenient features. This approach could be used as an initial step in developing new crosslinking treatments for patients with keratoconus.
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3Biomechanical Phenomena
4Care and treatment
5Chemical properties
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7Collagen - chemistry
8Collagen - drug effects
9Collagen - radiation effects
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abstractAims:To establish a non-destructive method of characterising the mechanical properties of collagen hydrogels to model corneal tissue and to examine the effect of photochemical crosslinking on their mechanical properties.Methods:Collagen hydrogels were manufactured, submerged in 0.1% riboflavin solution and crosslinked using two UVA tube bulbs with an intensity of between 2.8 and 3.2 mW/cm2. The hydrogels were clamped around their outer edge and deformed using a sphere. The deformation was measured in situ using a long-working-distance microscope connected to a CCD camera, and the deformation displacement was used with a theoretical model to calculate the Young modulus of the hydrogels. Collagen hydrogels seeded with human corneal fibroblasts were used to examine cell viability after UVA irradiation.Results:There was an increase in Young modulus of the collagen hydrogels after UVA/riboflavin treatment that was dependent on the exposure time. UVA irradiation without riboflavin showed decreased mechanical integrity and strength. Cell viability was reduced with increased UVA exposure time.Conclusion:The non-destructive technique demonstrated a new methodology comparable with strip extensiometry for cornea or corneal model specimens but with more convenient features. This approach could be used as an initial step in developing new crosslinking treatments for patients with keratoconus.
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