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Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots

Semiconductor quantum dots (QDs) are among the most promising emerging fluorescent labels for cellular imaging. However, it is unclear whether QDs, which are nanoparticles rather than small molecules, can specifically and effectively label molecular targets at a subcellular level. Here we have used... Full description

Journal Title: Nature biotechnology 2003, Vol.21 (1), p.41-46
Main Author: Wu, Xingyong
Other Authors: Liu, Hongjian , Liu, Jianquan , Haley, Kari N , Treadway, Joseph A , Larson, J Peter , Ge, Nianfeng , Peale, Frank , Bruchez, Marcel P
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: New York, NY: Nature
ID: ISSN: 1087-0156
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recordid: cdi_proquest_miscellaneous_72953112
title: Immunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots
format: Article
creator:
  • Wu, Xingyong
  • Liu, Hongjian
  • Liu, Jianquan
  • Haley, Kari N
  • Treadway, Joseph A
  • Larson, J Peter
  • Ge, Nianfeng
  • Peale, Frank
  • Bruchez, Marcel P
subjects:
  • 3T3 Cells - metabolism
  • 3T3 Cells - pathology
  • Animals
  • Antigens, Neoplasm - analysis
  • Biological and medical sciences
  • Biomarkers, Tumor - analysis
  • Biomarkers, Tumor - metabolism
  • Breast Neoplasms - metabolism
  • Breast Neoplasms - pathology
  • Crystallization - methods
  • Diagnostic Imaging - instrumentation
  • Diagnostic Imaging - methods
  • Diverse techniques
  • Electrochemistry
  • Fibroblasts - metabolism
  • Fibroblasts - pathology
  • Fluorescent Antibody Technique - methods
  • Fluorescent Dyes
  • Fundamental and applied biological sciences. Psychology
  • Humans
  • Mice
  • Microchemistry - methods
  • Microspheres
  • Molecular and cellular biology
  • Nanotechnology - methods
  • Receptor, ErbB-2 - analysis
  • Receptor, ErbB-2 - metabolism
  • Semiconductors
  • Spectrometry, Fluorescence - instrumentation
  • Spectrometry, Fluorescence - methods
  • Staining and Labeling - instrumentation
  • Staining and Labeling - methods
  • Tumor Cells, Cultured
ispartof: Nature biotechnology, 2003, Vol.21 (1), p.41-46
description: Semiconductor quantum dots (QDs) are among the most promising emerging fluorescent labels for cellular imaging. However, it is unclear whether QDs, which are nanoparticles rather than small molecules, can specifically and effectively label molecular targets at a subcellular level. Here we have used QDs linked to immunoglobulin G (IgG) and streptavidin to label the breast cancer marker Her2 on the surface of fixed and live cancer cells, to stain actin and microtubule fibers in the cytoplasm, and to detect nuclear antigens inside the nucleus. All labeling signals are specific for the intended targets and are brighter and considerably more photostable than comparable organic dyes. Using QDs with different emission spectra conjugated to IgG and streptavidin, we simultaneously detected two cellular targets with one excitation wavelength. The results indicate that QD-based probes can be very effective in cellular imaging and offer substantial advantages over organic dyes in multiplex target detection.
language: eng
source:
identifier: ISSN: 1087-0156
fulltext: no_fulltext
issn:
  • 1087-0156
  • 1546-1696
url: Link


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titleImmunofluorescent labeling of cancer marker Her2 and other cellular targets with semiconductor quantum dots
creatorWu, Xingyong ; Liu, Hongjian ; Liu, Jianquan ; Haley, Kari N ; Treadway, Joseph A ; Larson, J Peter ; Ge, Nianfeng ; Peale, Frank ; Bruchez, Marcel P
creatorcontribWu, Xingyong ; Liu, Hongjian ; Liu, Jianquan ; Haley, Kari N ; Treadway, Joseph A ; Larson, J Peter ; Ge, Nianfeng ; Peale, Frank ; Bruchez, Marcel P
descriptionSemiconductor quantum dots (QDs) are among the most promising emerging fluorescent labels for cellular imaging. However, it is unclear whether QDs, which are nanoparticles rather than small molecules, can specifically and effectively label molecular targets at a subcellular level. Here we have used QDs linked to immunoglobulin G (IgG) and streptavidin to label the breast cancer marker Her2 on the surface of fixed and live cancer cells, to stain actin and microtubule fibers in the cytoplasm, and to detect nuclear antigens inside the nucleus. All labeling signals are specific for the intended targets and are brighter and considerably more photostable than comparable organic dyes. Using QDs with different emission spectra conjugated to IgG and streptavidin, we simultaneously detected two cellular targets with one excitation wavelength. The results indicate that QD-based probes can be very effective in cellular imaging and offer substantial advantages over organic dyes in multiplex target detection.
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languageeng
publisherNew York, NY: Nature
subject3T3 Cells - metabolism ; 3T3 Cells - pathology ; Animals ; Antigens, Neoplasm - analysis ; Biological and medical sciences ; Biomarkers, Tumor - analysis ; Biomarkers, Tumor - metabolism ; Breast Neoplasms - metabolism ; Breast Neoplasms - pathology ; Crystallization - methods ; Diagnostic Imaging - instrumentation ; Diagnostic Imaging - methods ; Diverse techniques ; Electrochemistry ; Fibroblasts - metabolism ; Fibroblasts - pathology ; Fluorescent Antibody Technique - methods ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; Humans ; Mice ; Microchemistry - methods ; Microspheres ; Molecular and cellular biology ; Nanotechnology - methods ; Receptor, ErbB-2 - analysis ; Receptor, ErbB-2 - metabolism ; Semiconductors ; Spectrometry, Fluorescence - instrumentation ; Spectrometry, Fluorescence - methods ; Staining and Labeling - instrumentation ; Staining and Labeling - methods ; Tumor Cells, Cultured
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descriptionSemiconductor quantum dots (QDs) are among the most promising emerging fluorescent labels for cellular imaging. However, it is unclear whether QDs, which are nanoparticles rather than small molecules, can specifically and effectively label molecular targets at a subcellular level. Here we have used QDs linked to immunoglobulin G (IgG) and streptavidin to label the breast cancer marker Her2 on the surface of fixed and live cancer cells, to stain actin and microtubule fibers in the cytoplasm, and to detect nuclear antigens inside the nucleus. All labeling signals are specific for the intended targets and are brighter and considerably more photostable than comparable organic dyes. Using QDs with different emission spectra conjugated to IgG and streptavidin, we simultaneously detected two cellular targets with one excitation wavelength. The results indicate that QD-based probes can be very effective in cellular imaging and offer substantial advantages over organic dyes in multiplex target detection.
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03T3 Cells - metabolism
13T3 Cells - pathology
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5Biomarkers, Tumor - analysis
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authorWu, Xingyong ; Liu, Hongjian ; Liu, Jianquan ; Haley, Kari N ; Treadway, Joseph A ; Larson, J Peter ; Ge, Nianfeng ; Peale, Frank ; Bruchez, Marcel P
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03T3 Cells - metabolism
13T3 Cells - pathology
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3Antigens, Neoplasm - analysis
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7Breast Neoplasms - metabolism
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abstractSemiconductor quantum dots (QDs) are among the most promising emerging fluorescent labels for cellular imaging. However, it is unclear whether QDs, which are nanoparticles rather than small molecules, can specifically and effectively label molecular targets at a subcellular level. Here we have used QDs linked to immunoglobulin G (IgG) and streptavidin to label the breast cancer marker Her2 on the surface of fixed and live cancer cells, to stain actin and microtubule fibers in the cytoplasm, and to detect nuclear antigens inside the nucleus. All labeling signals are specific for the intended targets and are brighter and considerably more photostable than comparable organic dyes. Using QDs with different emission spectra conjugated to IgG and streptavidin, we simultaneously detected two cellular targets with one excitation wavelength. The results indicate that QD-based probes can be very effective in cellular imaging and offer substantial advantages over organic dyes in multiplex target detection.
copNew York, NY
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doi10.1038/nbt764
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