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Molecular cloning of alpha-amylases from cotton boll weevil, Anthonomus grandis and structural relations to plant inhibitors: an approach to insect resistance

Anthonomus grandis, the cotton boll weevil, causes severe cotton crop losses in North and South America. Here we demonstrate the presence of starch in the cotton pollen grains and young ovules that are the main A. grandis food source. We further demonstrate the presence of alpha-amylase activity, an... Full description

Journal Title: Journal of protein chemistry 2003-01, Vol.22 (1), p.77-88
Main Author: Oliveira-Neto, Osmundo B
Other Authors: Batista, João A N , Rigden, Daniel J , Franco, Octávio L , Falcão, Rosana , Fragoso, Rodrigo R , Mello, Luciane V , dos Santos, Roseane C , Grossi-de-Sá, Maria F
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: United States
ID: ISSN: 0277-8033
Link: https://www.ncbi.nlm.nih.gov/pubmed/12744224
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title: Molecular cloning of alpha-amylases from cotton boll weevil, Anthonomus grandis and structural relations to plant inhibitors: an approach to insect resistance
format: Article
creator:
  • Oliveira-Neto, Osmundo B
  • Batista, João A N
  • Rigden, Daniel J
  • Franco, Octávio L
  • Falcão, Rosana
  • Fragoso, Rodrigo R
  • Mello, Luciane V
  • dos Santos, Roseane C
  • Grossi-de-Sá, Maria F
subjects:
  • alpha-Amylases - antagonists & inhibitors
  • alpha-Amylases - chemistry
  • alpha-Amylases - isolation & purification
  • Amino Acid Sequence
  • Animals
  • Cloning, Molecular
  • Coleoptera - drug effects
  • Coleoptera - enzymology
  • DNA, Complementary - analysis
  • Enzyme Inhibitors - chemistry
  • Enzyme Inhibitors - isolation & purification
  • Enzyme Inhibitors - pharmacology
  • Insecticide Resistance
  • Larva - drug effects
  • Molecular Sequence Data
  • Plant Proteins - chemistry
  • Plant Proteins - isolation & purification
  • Plant Proteins - pharmacology
  • Secale - chemistry
  • Sequence Homology, Amino Acid
  • Triticum - chemistry
  • Trypsin Inhibitors
ispartof: Journal of protein chemistry, 2003-01, Vol.22 (1), p.77-88
description: Anthonomus grandis, the cotton boll weevil, causes severe cotton crop losses in North and South America. Here we demonstrate the presence of starch in the cotton pollen grains and young ovules that are the main A. grandis food source. We further demonstrate the presence of alpha-amylase activity, an essential enzyme of carbohydrate metabolism for many crop pests, in A. grandis midgut. Two alpha-amylase cDNAs from A. grandis larvae were isolated using RT-PCR followed by 5' and 3' RACE techniques. These encode proteins with predicted molecular masses of 50.8 and 52.7kDa, respectively, which share 58% amino acid identity. Expression of both genes is induced upon feeding and concentrated in the midgut of adult insects. Several alpha-amylase inhibitors from plants were assayed against A. grandis alpha-amylases but, unexpectedly, only the BIII inhibitor from rye kernels proved highly effective, with inhibitors generally active against other insect amylases lacking effect. Structural modeling of Amylag1 and Amylag2 showed that different factors seem to be responsible for the lack of effect of 0.19 and alpha-AI1 inhibitors on A. grandis alpha-amylase activity. This work suggests that genetic engineering of cotton to express alpha-amylase inhibitors may offer a novel route to A. grandis resistance.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0277-8033
fulltext: fulltext
issn:
  • 0277-8033
  • 1573-4943
url: Link


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titleMolecular cloning of alpha-amylases from cotton boll weevil, Anthonomus grandis and structural relations to plant inhibitors: an approach to insect resistance
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creatorOliveira-Neto, Osmundo B ; Batista, João A N ; Rigden, Daniel J ; Franco, Octávio L ; Falcão, Rosana ; Fragoso, Rodrigo R ; Mello, Luciane V ; dos Santos, Roseane C ; Grossi-de-Sá, Maria F
creatorcontribOliveira-Neto, Osmundo B ; Batista, João A N ; Rigden, Daniel J ; Franco, Octávio L ; Falcão, Rosana ; Fragoso, Rodrigo R ; Mello, Luciane V ; dos Santos, Roseane C ; Grossi-de-Sá, Maria F
descriptionAnthonomus grandis, the cotton boll weevil, causes severe cotton crop losses in North and South America. Here we demonstrate the presence of starch in the cotton pollen grains and young ovules that are the main A. grandis food source. We further demonstrate the presence of alpha-amylase activity, an essential enzyme of carbohydrate metabolism for many crop pests, in A. grandis midgut. Two alpha-amylase cDNAs from A. grandis larvae were isolated using RT-PCR followed by 5' and 3' RACE techniques. These encode proteins with predicted molecular masses of 50.8 and 52.7kDa, respectively, which share 58% amino acid identity. Expression of both genes is induced upon feeding and concentrated in the midgut of adult insects. Several alpha-amylase inhibitors from plants were assayed against A. grandis alpha-amylases but, unexpectedly, only the BIII inhibitor from rye kernels proved highly effective, with inhibitors generally active against other insect amylases lacking effect. Structural modeling of Amylag1 and Amylag2 showed that different factors seem to be responsible for the lack of effect of 0.19 and alpha-AI1 inhibitors on A. grandis alpha-amylase activity. This work suggests that genetic engineering of cotton to express alpha-amylase inhibitors may offer a novel route to A. grandis resistance.
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subjectalpha-Amylases - antagonists & inhibitors ; alpha-Amylases - chemistry ; alpha-Amylases - isolation & purification ; Amino Acid Sequence ; Animals ; Cloning, Molecular ; Coleoptera - drug effects ; Coleoptera - enzymology ; DNA, Complementary - analysis ; Enzyme Inhibitors - chemistry ; Enzyme Inhibitors - isolation & purification ; Enzyme Inhibitors - pharmacology ; Insecticide Resistance ; Larva - drug effects ; Molecular Sequence Data ; Plant Proteins - chemistry ; Plant Proteins - isolation & purification ; Plant Proteins - pharmacology ; Secale - chemistry ; Sequence Homology, Amino Acid ; Triticum - chemistry ; Trypsin Inhibitors
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6Mello, Luciane V
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8Grossi-de-Sá, Maria F
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0Molecular cloning of alpha-amylases from cotton boll weevil, Anthonomus grandis and structural relations to plant inhibitors: an approach to insect resistance
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descriptionAnthonomus grandis, the cotton boll weevil, causes severe cotton crop losses in North and South America. Here we demonstrate the presence of starch in the cotton pollen grains and young ovules that are the main A. grandis food source. We further demonstrate the presence of alpha-amylase activity, an essential enzyme of carbohydrate metabolism for many crop pests, in A. grandis midgut. Two alpha-amylase cDNAs from A. grandis larvae were isolated using RT-PCR followed by 5' and 3' RACE techniques. These encode proteins with predicted molecular masses of 50.8 and 52.7kDa, respectively, which share 58% amino acid identity. Expression of both genes is induced upon feeding and concentrated in the midgut of adult insects. Several alpha-amylase inhibitors from plants were assayed against A. grandis alpha-amylases but, unexpectedly, only the BIII inhibitor from rye kernels proved highly effective, with inhibitors generally active against other insect amylases lacking effect. Structural modeling of Amylag1 and Amylag2 showed that different factors seem to be responsible for the lack of effect of 0.19 and alpha-AI1 inhibitors on A. grandis alpha-amylase activity. This work suggests that genetic engineering of cotton to express alpha-amylase inhibitors may offer a novel route to A. grandis resistance.
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1alpha-Amylases - chemistry
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3Amino Acid Sequence
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6Coleoptera - drug effects
7Coleoptera - enzymology
8DNA, Complementary - analysis
9Enzyme Inhibitors - chemistry
10Enzyme Inhibitors - isolation & purification
11Enzyme Inhibitors - pharmacology
12Insecticide Resistance
13Larva - drug effects
14Molecular Sequence Data
15Plant Proteins - chemistry
16Plant Proteins - isolation & purification
17Plant Proteins - pharmacology
18Secale - chemistry
19Sequence Homology, Amino Acid
20Triticum - chemistry
21Trypsin Inhibitors
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titleMolecular cloning of alpha-amylases from cotton boll weevil, Anthonomus grandis and structural relations to plant inhibitors: an approach to insect resistance
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6Mello, Luciane V
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atitleMolecular cloning of alpha-amylases from cotton boll weevil, Anthonomus grandis and structural relations to plant inhibitors: an approach to insect resistance
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pages77-88
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abstractAnthonomus grandis, the cotton boll weevil, causes severe cotton crop losses in North and South America. Here we demonstrate the presence of starch in the cotton pollen grains and young ovules that are the main A. grandis food source. We further demonstrate the presence of alpha-amylase activity, an essential enzyme of carbohydrate metabolism for many crop pests, in A. grandis midgut. Two alpha-amylase cDNAs from A. grandis larvae were isolated using RT-PCR followed by 5' and 3' RACE techniques. These encode proteins with predicted molecular masses of 50.8 and 52.7kDa, respectively, which share 58% amino acid identity. Expression of both genes is induced upon feeding and concentrated in the midgut of adult insects. Several alpha-amylase inhibitors from plants were assayed against A. grandis alpha-amylases but, unexpectedly, only the BIII inhibitor from rye kernels proved highly effective, with inhibitors generally active against other insect amylases lacking effect. Structural modeling of Amylag1 and Amylag2 showed that different factors seem to be responsible for the lack of effect of 0.19 and alpha-AI1 inhibitors on A. grandis alpha-amylase activity. This work suggests that genetic engineering of cotton to express alpha-amylase inhibitors may offer a novel route to A. grandis resistance.
copUnited States
pmid12744224
doi10.1023/A:1023024012657