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Development of microbial populations in the anaerobic hydrolysis of grass silage for methane production

Abstract Six batch leach bed (LB) reactors, installed in parallel and connected to a common upflow anaerobic sludge blanket reactor, were fed with grass silage and operated at 35 (±1) °C. The development and distribution of microorganisms, which firmly and loosely attached to solid materials, and pr... Full description

Journal Title: FEMS microbiology ecology 2010-06-01, Vol.72 (3), p.496-506
Main Author: Wang, Hong
Other Authors: Vuorela, Mikko , Keränen, Anna-Leena , Lehtinen, Tuija M , Lensu, Anssi , Lehtomäki, Annimari , Rintala, Jukka
Format: Electronic Article Electronic Article
Language: English
Subjects:
RNA
Quelle: Alma/SFX Local Collection
Publisher: Oxford, UK: Blackwell Publishing Ltd
ID: ISSN: 0168-6496
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title: Development of microbial populations in the anaerobic hydrolysis of grass silage for methane production
format: Article
creator:
  • Wang, Hong
  • Vuorela, Mikko
  • Keränen, Anna-Leena
  • Lehtinen, Tuija M
  • Lensu, Anssi
  • Lehtomäki, Annimari
  • Rintala, Jukka
subjects:
  • Anaerobic microorganisms
  • Anaerobiosis
  • Animal, plant and microbial ecology
  • Archaea
  • Archaea - classification
  • Archaea - genetics
  • Archaea - isolation & purification
  • Bacteria
  • Bacteria - classification
  • Bacteria - genetics
  • Bacteria - isolation & purification
  • Bacterial leaching
  • Bacteroidetes
  • Biological and medical sciences
  • Bioreactors
  • Cellulose
  • cellulose hydrolysis
  • clone library
  • Clostridia
  • Communities
  • DNA, Archaeal - genetics
  • DNA, Bacterial - genetics
  • Fundamental and applied biological sciences. Psychology
  • Gene polymorphism
  • Grasses
  • Hydrolysis
  • Leachates
  • Methane
  • Methane - biosynthesis
  • Methanobacterium
  • Methanosarcina
  • microbial community
  • Microbial ecology
  • Microorganisms
  • Phylogeny
  • Poaceae
  • Polymorphism
  • Polymorphism, Restriction Fragment Length
  • Reactors
  • Relative abundance
  • Residues
  • Restriction fragment length polymorphism
  • RNA
  • RNA, Ribosomal, 16S - genetics
  • rRNA 16S
  • Sewage - microbiology
  • Silage
  • Silage - microbiology
  • Sludge
  • terminal restriction fragment length polymorphism
  • two‐stage anaerobic digestion
  • Upflow anaerobic sludge blanket reactors
  • Various environments (extraatmospheric space, air, water)
ispartof: FEMS microbiology ecology, 2010-06-01, Vol.72 (3), p.496-506
description: Abstract Six batch leach bed (LB) reactors, installed in parallel and connected to a common upflow anaerobic sludge blanket reactor, were fed with grass silage and operated at 35 (±1) °C. The development and distribution of microorganisms, which firmly and loosely attached to solid materials, and presented in the leachate in the LB reactors, were investigated by 16S rRNA gene-based terminal restriction fragment length polymorphism and clone library analyses. The phylotypes and their relative abundance changed in the respective bacterial community throughout the 49-day run and showed differences between the communities. Large numbers of phylotypes were detected from day 10 onwards. On day 17, the majority of phylotypes in the bacterial community firmly attached to solid residues affiliated to the classes Clostridia and Bacteroidetes. There were high numbers of the phylotypes in the leachate bacterial community. They were closely related to members of classes Clostridia, Bacteroidetes, Betaproteobacteria, Alphaproteobacteria, Gammaproteobacteria, and OP10. The Clostridium-like species clearly dominated the bacterial community. Archaea were only found in the solid residues on day 17 and in the leachate on days 10 and 17. The majority of the Archaea fell within the hydrogenotrophic genus Methanobacterium. The organism assigned to the aceticlastic genus Methanosarcina was only present in the solid residues.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0168-6496
fulltext: fulltext
issn:
  • 0168-6496
  • 1574-6941
url: Link


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titleDevelopment of microbial populations in the anaerobic hydrolysis of grass silage for methane production
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creatorWang, Hong ; Vuorela, Mikko ; Keränen, Anna-Leena ; Lehtinen, Tuija M ; Lensu, Anssi ; Lehtomäki, Annimari ; Rintala, Jukka
creatorcontribWang, Hong ; Vuorela, Mikko ; Keränen, Anna-Leena ; Lehtinen, Tuija M ; Lensu, Anssi ; Lehtomäki, Annimari ; Rintala, Jukka
descriptionAbstract Six batch leach bed (LB) reactors, installed in parallel and connected to a common upflow anaerobic sludge blanket reactor, were fed with grass silage and operated at 35 (±1) °C. The development and distribution of microorganisms, which firmly and loosely attached to solid materials, and presented in the leachate in the LB reactors, were investigated by 16S rRNA gene-based terminal restriction fragment length polymorphism and clone library analyses. The phylotypes and their relative abundance changed in the respective bacterial community throughout the 49-day run and showed differences between the communities. Large numbers of phylotypes were detected from day 10 onwards. On day 17, the majority of phylotypes in the bacterial community firmly attached to solid residues affiliated to the classes Clostridia and Bacteroidetes. There were high numbers of the phylotypes in the leachate bacterial community. They were closely related to members of classes Clostridia, Bacteroidetes, Betaproteobacteria, Alphaproteobacteria, Gammaproteobacteria, and OP10. The Clostridium-like species clearly dominated the bacterial community. Archaea were only found in the solid residues on day 17 and in the leachate on days 10 and 17. The majority of the Archaea fell within the hydrogenotrophic genus Methanobacterium. The organism assigned to the aceticlastic genus Methanosarcina was only present in the solid residues.
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languageeng
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subjectAnaerobic microorganisms ; Anaerobiosis ; Animal, plant and microbial ecology ; Archaea ; Archaea - classification ; Archaea - genetics ; Archaea - isolation & purification ; Bacteria ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacterial leaching ; Bacteroidetes ; Biological and medical sciences ; Bioreactors ; Cellulose ; cellulose hydrolysis ; clone library ; Clostridia ; Communities ; DNA, Archaeal - genetics ; DNA, Bacterial - genetics ; Fundamental and applied biological sciences. Psychology ; Gene polymorphism ; Grasses ; Hydrolysis ; Leachates ; Methane ; Methane - biosynthesis ; Methanobacterium ; Methanosarcina ; microbial community ; Microbial ecology ; Microorganisms ; Phylogeny ; Poaceae ; Polymorphism ; Polymorphism, Restriction Fragment Length ; Reactors ; Relative abundance ; Residues ; Restriction fragment length polymorphism ; RNA ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Sewage - microbiology ; Silage ; Silage - microbiology ; Sludge ; terminal restriction fragment length polymorphism ; two‐stage anaerobic digestion ; Upflow anaerobic sludge blanket reactors ; Various environments (extraatmospheric space, air, water)
ispartofFEMS microbiology ecology, 2010-06-01, Vol.72 (3), p.496-506
rights
02010 Federation of European Microbiological Societies 2010
12010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
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1Vuorela, Mikko
2Keränen, Anna-Leena
3Lehtinen, Tuija M
4Lensu, Anssi
5Lehtomäki, Annimari
6Rintala, Jukka
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0Development of microbial populations in the anaerobic hydrolysis of grass silage for methane production
1FEMS microbiology ecology
addtitleFEMS Microbiol Ecol
descriptionAbstract Six batch leach bed (LB) reactors, installed in parallel and connected to a common upflow anaerobic sludge blanket reactor, were fed with grass silage and operated at 35 (±1) °C. The development and distribution of microorganisms, which firmly and loosely attached to solid materials, and presented in the leachate in the LB reactors, were investigated by 16S rRNA gene-based terminal restriction fragment length polymorphism and clone library analyses. The phylotypes and their relative abundance changed in the respective bacterial community throughout the 49-day run and showed differences between the communities. Large numbers of phylotypes were detected from day 10 onwards. On day 17, the majority of phylotypes in the bacterial community firmly attached to solid residues affiliated to the classes Clostridia and Bacteroidetes. There were high numbers of the phylotypes in the leachate bacterial community. They were closely related to members of classes Clostridia, Bacteroidetes, Betaproteobacteria, Alphaproteobacteria, Gammaproteobacteria, and OP10. The Clostridium-like species clearly dominated the bacterial community. Archaea were only found in the solid residues on day 17 and in the leachate on days 10 and 17. The majority of the Archaea fell within the hydrogenotrophic genus Methanobacterium. The organism assigned to the aceticlastic genus Methanosarcina was only present in the solid residues.
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0Anaerobic microorganisms
1Anaerobiosis
2Animal, plant and microbial ecology
3Archaea
4Archaea - classification
5Archaea - genetics
6Archaea - isolation & purification
7Bacteria
8Bacteria - classification
9Bacteria - genetics
10Bacteria - isolation & purification
11Bacterial leaching
12Bacteroidetes
13Biological and medical sciences
14Bioreactors
15Cellulose
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17clone library
18Clostridia
19Communities
20DNA, Archaeal - genetics
21DNA, Bacterial - genetics
22Fundamental and applied biological sciences. Psychology
23Gene polymorphism
24Grasses
25Hydrolysis
26Leachates
27Methane
28Methane - biosynthesis
29Methanobacterium
30Methanosarcina
31microbial community
32Microbial ecology
33Microorganisms
34Phylogeny
35Poaceae
36Polymorphism
37Polymorphism, Restriction Fragment Length
38Reactors
39Relative abundance
40Residues
41Restriction fragment length polymorphism
42RNA
43RNA, Ribosomal, 16S - genetics
44rRNA 16S
45Sewage - microbiology
46Silage
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48Sludge
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50two‐stage anaerobic digestion
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52Various environments (extraatmospheric space, air, water)
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3Archaea
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10Bacteria - isolation & purification
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12Bacteroidetes
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15Cellulose
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21DNA, Bacterial - genetics
22Fundamental and applied biological sciences. Psychology
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26Leachates
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atitleDevelopment of microbial populations in the anaerobic hydrolysis of grass silage for methane production
jtitleFEMS microbiology ecology
addtitleFEMS Microbiol Ecol
date2010-06-01
risdate2010
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issue3
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pages496-506
issn0168-6496
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notesEditor: Gary King
abstractAbstract Six batch leach bed (LB) reactors, installed in parallel and connected to a common upflow anaerobic sludge blanket reactor, were fed with grass silage and operated at 35 (±1) °C. The development and distribution of microorganisms, which firmly and loosely attached to solid materials, and presented in the leachate in the LB reactors, were investigated by 16S rRNA gene-based terminal restriction fragment length polymorphism and clone library analyses. The phylotypes and their relative abundance changed in the respective bacterial community throughout the 49-day run and showed differences between the communities. Large numbers of phylotypes were detected from day 10 onwards. On day 17, the majority of phylotypes in the bacterial community firmly attached to solid residues affiliated to the classes Clostridia and Bacteroidetes. There were high numbers of the phylotypes in the leachate bacterial community. They were closely related to members of classes Clostridia, Bacteroidetes, Betaproteobacteria, Alphaproteobacteria, Gammaproteobacteria, and OP10. The Clostridium-like species clearly dominated the bacterial community. Archaea were only found in the solid residues on day 17 and in the leachate on days 10 and 17. The majority of the Archaea fell within the hydrogenotrophic genus Methanobacterium. The organism assigned to the aceticlastic genus Methanosarcina was only present in the solid residues.
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