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Interleukin-12 p40 Gene Expression Is Induced in Lipopolysaccharide-Activated Pituitary Glands in vivo

Proinflammatory cytokines have several functions including activation of the hypothalamo-pituitary-adrenal (HPA) axis and regulation of the immune system. The present study focuses on the regulation of interleukin 12 (IL-12) and its receptor gene expression in the HPA axis under artificially induced... Full description

Journal Title: Neuroendocrinology 2002, Vol.75 (6), p.347-357
Main Author: Yang, Young
Other Authors: Han, Seung Hyun , Kim, Hyun , Kim, Changmee , Kim, Kun-yong , Shin, Sun Mi , Choi, Inpyo , Pyun, Kwang Ho
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Basel, Switzerland: S. Karger AG
ID: ISSN: 0028-3835
Link: https://www.ncbi.nlm.nih.gov/pubmed/12065887
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recordid: cdi_proquest_miscellaneous_746231261
title: Interleukin-12 p40 Gene Expression Is Induced in Lipopolysaccharide-Activated Pituitary Glands in vivo
format: Article
creator:
  • Yang, Young
  • Han, Seung Hyun
  • Kim, Hyun
  • Kim, Changmee
  • Kim, Kun-yong
  • Shin, Sun Mi
  • Choi, Inpyo
  • Pyun, Kwang Ho
subjects:
  • Animals
  • Blotting, Northern
  • Corticotropin Releasing Hormone and Corticotropin
  • DNA - metabolism
  • Gene Expression - drug effects
  • In Situ Hybridization
  • Interleukin-12 - biosynthesis
  • Interleukin-12 - genetics
  • Interleukin-12 Subunit p40
  • Lipopolysaccharide Receptors - genetics
  • Lipopolysaccharides - administration & dosage
  • Lipopolysaccharides - pharmacology
  • Male
  • NF-kappa B - metabolism
  • Organ Culture Techniques
  • Pituitary Gland - metabolism
  • Pituitary Gland, Anterior - chemistry
  • Protein Subunits
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Adrenergic, beta - physiology
  • Reverse Transcriptase Polymerase Chain Reaction
  • RNA, Messenger - analysis
  • Transcription Factor AP-1 - metabolism
ispartof: Neuroendocrinology, 2002, Vol.75 (6), p.347-357
description: Proinflammatory cytokines have several functions including activation of the hypothalamo-pituitary-adrenal (HPA) axis and regulation of the immune system. The present study focuses on the regulation of interleukin 12 (IL-12) and its receptor gene expression in the HPA axis under artificially induced immune stress, brought on by administration of lipopolysaccharide (LPS) to Sprague-Dawley (SD) rats. RT-PCR analyses showed that expression of the IL-12 p40 gene was significantly increased and peaked at 2 h in the pituitary gland, but not in the hypothalamus. LPS-induced IL-12 p40 gene induction in the pituitary gland was suppressed after β-adrenoceptor agonist pretreatment in vivo. Both IL-12 p40 gene induction and IL-12 production were also observed when freshly isolated pituitary glands from non-treated SD rats were incubated with LPS in vitro. Furthermore, CD14, which is known as a LPS receptor, was found to be expressed in the pituitary gland. Gel mobility shift assays using nuclear extracts prepared from the pituitary glands of rats administered LPS showed induction of NF-ĸB and AP-1 DNA-binding activity. These results suggest that LPS stimulates the pituitary gland directly in vivo to increase IL-12 p40 gene expression and IL-12 protein production.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0028-3835
fulltext: fulltext
issn:
  • 0028-3835
  • 1423-0194
url: Link


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titleInterleukin-12 p40 Gene Expression Is Induced in Lipopolysaccharide-Activated Pituitary Glands in vivo
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creatorYang, Young ; Han, Seung Hyun ; Kim, Hyun ; Kim, Changmee ; Kim, Kun-yong ; Shin, Sun Mi ; Choi, Inpyo ; Pyun, Kwang Ho
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descriptionProinflammatory cytokines have several functions including activation of the hypothalamo-pituitary-adrenal (HPA) axis and regulation of the immune system. The present study focuses on the regulation of interleukin 12 (IL-12) and its receptor gene expression in the HPA axis under artificially induced immune stress, brought on by administration of lipopolysaccharide (LPS) to Sprague-Dawley (SD) rats. RT-PCR analyses showed that expression of the IL-12 p40 gene was significantly increased and peaked at 2 h in the pituitary gland, but not in the hypothalamus. LPS-induced IL-12 p40 gene induction in the pituitary gland was suppressed after β-adrenoceptor agonist pretreatment in vivo. Both IL-12 p40 gene induction and IL-12 production were also observed when freshly isolated pituitary glands from non-treated SD rats were incubated with LPS in vitro. Furthermore, CD14, which is known as a LPS receptor, was found to be expressed in the pituitary gland. Gel mobility shift assays using nuclear extracts prepared from the pituitary glands of rats administered LPS showed induction of NF-ĸB and AP-1 DNA-binding activity. These results suggest that LPS stimulates the pituitary gland directly in vivo to increase IL-12 p40 gene expression and IL-12 protein production.
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subjectAnimals ; Blotting, Northern ; Corticotropin Releasing Hormone and Corticotropin ; DNA - metabolism ; Gene Expression - drug effects ; In Situ Hybridization ; Interleukin-12 - biosynthesis ; Interleukin-12 - genetics ; Interleukin-12 Subunit p40 ; Lipopolysaccharide Receptors - genetics ; Lipopolysaccharides - administration & dosage ; Lipopolysaccharides - pharmacology ; Male ; NF-kappa B - metabolism ; Organ Culture Techniques ; Pituitary Gland - metabolism ; Pituitary Gland, Anterior - chemistry ; Protein Subunits ; Rats ; Rats, Sprague-Dawley ; Receptors, Adrenergic, beta - physiology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; Transcription Factor AP-1 - metabolism
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descriptionProinflammatory cytokines have several functions including activation of the hypothalamo-pituitary-adrenal (HPA) axis and regulation of the immune system. The present study focuses on the regulation of interleukin 12 (IL-12) and its receptor gene expression in the HPA axis under artificially induced immune stress, brought on by administration of lipopolysaccharide (LPS) to Sprague-Dawley (SD) rats. RT-PCR analyses showed that expression of the IL-12 p40 gene was significantly increased and peaked at 2 h in the pituitary gland, but not in the hypothalamus. LPS-induced IL-12 p40 gene induction in the pituitary gland was suppressed after β-adrenoceptor agonist pretreatment in vivo. Both IL-12 p40 gene induction and IL-12 production were also observed when freshly isolated pituitary glands from non-treated SD rats were incubated with LPS in vitro. Furthermore, CD14, which is known as a LPS receptor, was found to be expressed in the pituitary gland. Gel mobility shift assays using nuclear extracts prepared from the pituitary glands of rats administered LPS showed induction of NF-ĸB and AP-1 DNA-binding activity. These results suggest that LPS stimulates the pituitary gland directly in vivo to increase IL-12 p40 gene expression and IL-12 protein production.
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abstractProinflammatory cytokines have several functions including activation of the hypothalamo-pituitary-adrenal (HPA) axis and regulation of the immune system. The present study focuses on the regulation of interleukin 12 (IL-12) and its receptor gene expression in the HPA axis under artificially induced immune stress, brought on by administration of lipopolysaccharide (LPS) to Sprague-Dawley (SD) rats. RT-PCR analyses showed that expression of the IL-12 p40 gene was significantly increased and peaked at 2 h in the pituitary gland, but not in the hypothalamus. LPS-induced IL-12 p40 gene induction in the pituitary gland was suppressed after β-adrenoceptor agonist pretreatment in vivo. Both IL-12 p40 gene induction and IL-12 production were also observed when freshly isolated pituitary glands from non-treated SD rats were incubated with LPS in vitro. Furthermore, CD14, which is known as a LPS receptor, was found to be expressed in the pituitary gland. Gel mobility shift assays using nuclear extracts prepared from the pituitary glands of rats administered LPS showed induction of NF-ĸB and AP-1 DNA-binding activity. These results suggest that LPS stimulates the pituitary gland directly in vivo to increase IL-12 p40 gene expression and IL-12 protein production.
copBasel, Switzerland
pubS. Karger AG
pmid12065887
doi10.1159/000059431
tpages11