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Insulin-like growth factor binding protein-3 protease activity in the urine of children with chronic renal failure

The insulin-like growth factors, IGF-1 and IGF-II, are polypeptides that potentiate cellular growth. In addition to binding to specific cell surface receptors, the IGFs bind with high affinity to a family of proteins, the insulin-like growth factor binding proteins (IGFBPs). Serum and urine IGFBP pa... Full description

Journal Title: Pediatric nephrology (Berlin West), 1993, Vol.7 (4), p.416-423
Main Author: DAE-YEOL LEE
Other Authors: COHEN, P , KRENSKY, A. M , ROSENFELD, R. G , YORGIN, P. D
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Heidelberg: Springer
ID: ISSN: 0931-041X
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recordid: cdi_proquest_miscellaneous_75982445
title: Insulin-like growth factor binding protein-3 protease activity in the urine of children with chronic renal failure
format: Article
creator:
  • DAE-YEOL LEE
  • COHEN, P
  • KRENSKY, A. M
  • ROSENFELD, R. G
  • YORGIN, P. D
subjects:
  • Adolescent
  • Biological and medical sciences
  • Blotting, Western
  • Carrier Proteins - blood
  • Carrier Proteins - urine
  • Child
  • Child, Preschool
  • Endopeptidases - blood
  • Endopeptidases - urine
  • Female
  • Growth Inhibitors - blood
  • Growth Inhibitors - urine
  • Humans
  • Infant
  • Insulin-Like Growth Factor Binding Proteins
  • Kidney Failure, Chronic - blood
  • Kidney Failure, Chronic - enzymology
  • Kidney Failure, Chronic - urine
  • Male
  • Medical sciences
  • Nephrology. Urinary tract diseases
  • Nephropathies. Renovascular diseases. Renal failure
  • Radioimmunoassay
  • Renal failure
  • Somatomedins - urine
ispartof: Pediatric nephrology (Berlin, West), 1993, Vol.7 (4), p.416-423
description: The insulin-like growth factors, IGF-1 and IGF-II, are polypeptides that potentiate cellular growth. In addition to binding to specific cell surface receptors, the IGFs bind with high affinity to a family of proteins, the insulin-like growth factor binding proteins (IGFBPs). Serum and urine IGFBP patterns are altered in individuals with chronic renal failure (CRF). We recently reported that the urinary IGFBP pattern of CRF patients is unique for increased insulin-like growth factor binding protein-1 (U-IGFBP-1) levels. In this study, we used western ligand blotting (WLB), western immunoblotting (WIB), and radioimmunoassay (RIA) to further evaluate serum and urine IGFBP profiles of children with CRF (n = 14). Five patients with CRF displayed decreased serum IGFBP-3 profiles by WLB. Serum IGFBP-3 WIB profiles were remarkable for 30- and 20-kDa fragments of IGFBP-3 not seen in control serum. Serum IGFBP-3 levels, as determined by RIA, were slightly elevated. Serum levels of IGFBP-2 also were increased, although not at a level reaching statistical significance. WLB of CRF urine revealed a large increase in U-IGFBP-1 and a complete absence of urinary IGFBP-3. Recent studies of serum from pregnant women and seminal plasma have demonstrated a similar absence of intact IGFBP-3, due to the presence of a specific IGFBP-3 protease. To evaluate whether an IGFBP-3 protease accounts for the absence of intact U-IGFBP-3 in children with CRF, urine and serum samples from individuals with CRF and controls were tested.
language: eng
source:
identifier: ISSN: 0931-041X
fulltext: no_fulltext
issn:
  • 0931-041X
  • 1432-198X
url: Link


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titleInsulin-like growth factor binding protein-3 protease activity in the urine of children with chronic renal failure
creatorDAE-YEOL LEE ; COHEN, P ; KRENSKY, A. M ; ROSENFELD, R. G ; YORGIN, P. D
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descriptionThe insulin-like growth factors, IGF-1 and IGF-II, are polypeptides that potentiate cellular growth. In addition to binding to specific cell surface receptors, the IGFs bind with high affinity to a family of proteins, the insulin-like growth factor binding proteins (IGFBPs). Serum and urine IGFBP patterns are altered in individuals with chronic renal failure (CRF). We recently reported that the urinary IGFBP pattern of CRF patients is unique for increased insulin-like growth factor binding protein-1 (U-IGFBP-1) levels. In this study, we used western ligand blotting (WLB), western immunoblotting (WIB), and radioimmunoassay (RIA) to further evaluate serum and urine IGFBP profiles of children with CRF (n = 14). Five patients with CRF displayed decreased serum IGFBP-3 profiles by WLB. Serum IGFBP-3 WIB profiles were remarkable for 30- and 20-kDa fragments of IGFBP-3 not seen in control serum. Serum IGFBP-3 levels, as determined by RIA, were slightly elevated. Serum levels of IGFBP-2 also were increased, although not at a level reaching statistical significance. WLB of CRF urine revealed a large increase in U-IGFBP-1 and a complete absence of urinary IGFBP-3. Recent studies of serum from pregnant women and seminal plasma have demonstrated a similar absence of intact IGFBP-3, due to the presence of a specific IGFBP-3 protease. To evaluate whether an IGFBP-3 protease accounts for the absence of intact U-IGFBP-3 in children with CRF, urine and serum samples from individuals with CRF and controls were tested.
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languageeng
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subjectAdolescent ; Biological and medical sciences ; Blotting, Western ; Carrier Proteins - blood ; Carrier Proteins - urine ; Child ; Child, Preschool ; Endopeptidases - blood ; Endopeptidases - urine ; Female ; Growth Inhibitors - blood ; Growth Inhibitors - urine ; Humans ; Infant ; Insulin-Like Growth Factor Binding Proteins ; Kidney Failure, Chronic - blood ; Kidney Failure, Chronic - enzymology ; Kidney Failure, Chronic - urine ; Male ; Medical sciences ; Nephrology. Urinary tract diseases ; Nephropathies. Renovascular diseases. Renal failure ; Radioimmunoassay ; Renal failure ; Somatomedins - urine
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1Pediatric nephrology (Berlin, West)
addtitlePediatr Nephrol
descriptionThe insulin-like growth factors, IGF-1 and IGF-II, are polypeptides that potentiate cellular growth. In addition to binding to specific cell surface receptors, the IGFs bind with high affinity to a family of proteins, the insulin-like growth factor binding proteins (IGFBPs). Serum and urine IGFBP patterns are altered in individuals with chronic renal failure (CRF). We recently reported that the urinary IGFBP pattern of CRF patients is unique for increased insulin-like growth factor binding protein-1 (U-IGFBP-1) levels. In this study, we used western ligand blotting (WLB), western immunoblotting (WIB), and radioimmunoassay (RIA) to further evaluate serum and urine IGFBP profiles of children with CRF (n = 14). Five patients with CRF displayed decreased serum IGFBP-3 profiles by WLB. Serum IGFBP-3 WIB profiles were remarkable for 30- and 20-kDa fragments of IGFBP-3 not seen in control serum. Serum IGFBP-3 levels, as determined by RIA, were slightly elevated. Serum levels of IGFBP-2 also were increased, although not at a level reaching statistical significance. WLB of CRF urine revealed a large increase in U-IGFBP-1 and a complete absence of urinary IGFBP-3. Recent studies of serum from pregnant women and seminal plasma have demonstrated a similar absence of intact IGFBP-3, due to the presence of a specific IGFBP-3 protease. To evaluate whether an IGFBP-3 protease accounts for the absence of intact U-IGFBP-3 in children with CRF, urine and serum samples from individuals with CRF and controls were tested.
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1Biological and medical sciences
2Blotting, Western
3Carrier Proteins - blood
4Carrier Proteins - urine
5Child
6Child, Preschool
7Endopeptidases - blood
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9Female
10Growth Inhibitors - blood
11Growth Inhibitors - urine
12Humans
13Infant
14Insulin-Like Growth Factor Binding Proteins
15Kidney Failure, Chronic - blood
16Kidney Failure, Chronic - enzymology
17Kidney Failure, Chronic - urine
18Male
19Medical sciences
20Nephrology. Urinary tract diseases
21Nephropathies. Renovascular diseases. Renal failure
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titleInsulin-like growth factor binding protein-3 protease activity in the urine of children with chronic renal failure
authorDAE-YEOL LEE ; COHEN, P ; KRENSKY, A. M ; ROSENFELD, R. G ; YORGIN, P. D
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14Insulin-Like Growth Factor Binding Proteins
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19Medical sciences
20Nephrology. Urinary tract diseases
21Nephropathies. Renovascular diseases. Renal failure
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24Somatomedins - urine
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abstractThe insulin-like growth factors, IGF-1 and IGF-II, are polypeptides that potentiate cellular growth. In addition to binding to specific cell surface receptors, the IGFs bind with high affinity to a family of proteins, the insulin-like growth factor binding proteins (IGFBPs). Serum and urine IGFBP patterns are altered in individuals with chronic renal failure (CRF). We recently reported that the urinary IGFBP pattern of CRF patients is unique for increased insulin-like growth factor binding protein-1 (U-IGFBP-1) levels. In this study, we used western ligand blotting (WLB), western immunoblotting (WIB), and radioimmunoassay (RIA) to further evaluate serum and urine IGFBP profiles of children with CRF (n = 14). Five patients with CRF displayed decreased serum IGFBP-3 profiles by WLB. Serum IGFBP-3 WIB profiles were remarkable for 30- and 20-kDa fragments of IGFBP-3 not seen in control serum. Serum IGFBP-3 levels, as determined by RIA, were slightly elevated. Serum levels of IGFBP-2 also were increased, although not at a level reaching statistical significance. WLB of CRF urine revealed a large increase in U-IGFBP-1 and a complete absence of urinary IGFBP-3. Recent studies of serum from pregnant women and seminal plasma have demonstrated a similar absence of intact IGFBP-3, due to the presence of a specific IGFBP-3 protease. To evaluate whether an IGFBP-3 protease accounts for the absence of intact U-IGFBP-3 in children with CRF, urine and serum samples from individuals with CRF and controls were tested.
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