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Impact of Long-Term Fertilization on the Composition of Denitrifier Communities Based on Nitrite Reductase Analyses in a Paddy Soil

The effect of long-term fertilization on soildenitrifying communities was determined by measuring the abundance and diversity of the nitrite reductase genes nirK and nirS. Soil samples were collected from plots of a long-term fertilization experiment started in 1990, located in Taoyuan (110°72" E, 2... Full description

Journal Title: Microbial ecology 2010-11-01, Vol.60 (4), p.850-861
Main Author: Chen, Zhe
Other Authors: Luo, Xiqian , Hu, Ronggui , Wu, Minna , Wu, Jinshui , Wei, Wenxue
Format: Electronic Article Electronic Article
Language: English
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Publisher: New York: Springer Science + Business Media, Inc
ID: ISSN: 0095-3628
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recordid: cdi_proquest_miscellaneous_762687647
title: Impact of Long-Term Fertilization on the Composition of Denitrifier Communities Based on Nitrite Reductase Analyses in a Paddy Soil
format: Article
creator:
  • Chen, Zhe
  • Luo, Xiqian
  • Hu, Ronggui
  • Wu, Minna
  • Wu, Jinshui
  • Wei, Wenxue
subjects:
  • Agricultural site preparation
  • Bacteria - classification
  • Bacteria - enzymology
  • Bacteria - genetics
  • Bacteria - isolation & purification
  • Bacterial Proteins - genetics
  • Biological and medical sciences
  • Biomedical and Life Sciences
  • Carbon content
  • Ecology
  • Fertilization
  • Fertilizers
  • Fertilizers - analysis
  • Fundamental and applied biological sciences. Psychology
  • Gene Dosage
  • Geoecology/Natural Processes
  • Life Sciences
  • Microbial Ecology
  • Microbiology
  • Molecular Sequence Data
  • Nature Conservation
  • Nitrite Reductases - genetics
  • Nitrites
  • Paddy soils
  • Phylogenetics
  • Phylogeny
  • Polymerase chain reaction
  • Sedimentary soils
  • Soil - analysis
  • Soil composition
  • SOIL MICROBIOLOGY
  • Soil microorganisms
  • Soil samples
  • Soils
  • Universities and colleges
  • Urea
  • Water Quality/Water Pollution
ispartof: Microbial ecology, 2010-11-01, Vol.60 (4), p.850-861
description: The effect of long-term fertilization on soildenitrifying communities was determined by measuring the abundance and diversity of the nitrite reductase genes nirK and nirS. Soil samples were collected from plots of a long-term fertilization experiment started in 1990, located in Taoyuan (110°72" E, 28°52" N), China. The treatments were no fertilizer (NF), urea (UR), balanced mineral fertilizers (BM), and BM combined with rice straw (BMR). The abundance, diversity, and composition of the soil-denitrifying bacteria were determined by using realtime quantitative PCR, terminal restriction fragment length polymorphism (T-RFLP), and cloning and sequencing of nirK and nirS genes. There was a pronounced difference in the community composition and diversity of nirK-containing denitrifiers responding to the long-term fertilization regimes; however, less variation was observed in communities of nirS-containing denitrifiers, indicating that denitrifiers possessing nirK were more sensitive to the fertilization practices than those with nirS. In contrast, fertilization regimes had similar effects on the copy numbers of nirK and nirS genes. The BMR treatment had the highest copy numbers of nirK and nirS, followed by the two mineral fertilization regimes (UR and BM), and the lowest was in the NF treatment. Of the measured soil parameters, the differences in the community composition of nirK and the abundance of nir denitrifiers were highly correlated with the soil carbon content. Therefore, longterm fertilization resulted in a strong impact on the community structure of nirK populations only, and total organic carbon was the dominant factor in relation to the variations of nir community sizes.
language: eng
source:
identifier: ISSN: 0095-3628
fulltext: no_fulltext
issn:
  • 0095-3628
  • 1432-184X
url: Link


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titleImpact of Long-Term Fertilization on the Composition of Denitrifier Communities Based on Nitrite Reductase Analyses in a Paddy Soil
creatorChen, Zhe ; Luo, Xiqian ; Hu, Ronggui ; Wu, Minna ; Wu, Jinshui ; Wei, Wenxue
creatorcontribChen, Zhe ; Luo, Xiqian ; Hu, Ronggui ; Wu, Minna ; Wu, Jinshui ; Wei, Wenxue
descriptionThe effect of long-term fertilization on soildenitrifying communities was determined by measuring the abundance and diversity of the nitrite reductase genes nirK and nirS. Soil samples were collected from plots of a long-term fertilization experiment started in 1990, located in Taoyuan (110°72" E, 28°52" N), China. The treatments were no fertilizer (NF), urea (UR), balanced mineral fertilizers (BM), and BM combined with rice straw (BMR). The abundance, diversity, and composition of the soil-denitrifying bacteria were determined by using realtime quantitative PCR, terminal restriction fragment length polymorphism (T-RFLP), and cloning and sequencing of nirK and nirS genes. There was a pronounced difference in the community composition and diversity of nirK-containing denitrifiers responding to the long-term fertilization regimes; however, less variation was observed in communities of nirS-containing denitrifiers, indicating that denitrifiers possessing nirK were more sensitive to the fertilization practices than those with nirS. In contrast, fertilization regimes had similar effects on the copy numbers of nirK and nirS genes. The BMR treatment had the highest copy numbers of nirK and nirS, followed by the two mineral fertilization regimes (UR and BM), and the lowest was in the NF treatment. Of the measured soil parameters, the differences in the community composition of nirK and the abundance of nir denitrifiers were highly correlated with the soil carbon content. Therefore, longterm fertilization resulted in a strong impact on the community structure of nirK populations only, and total organic carbon was the dominant factor in relation to the variations of nir community sizes.
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subjectAgricultural site preparation ; Bacteria - classification ; Bacteria - enzymology ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacterial Proteins - genetics ; Biological and medical sciences ; Biomedical and Life Sciences ; Carbon content ; Ecology ; Fertilization ; Fertilizers ; Fertilizers - analysis ; Fundamental and applied biological sciences. Psychology ; Gene Dosage ; Geoecology/Natural Processes ; Life Sciences ; Microbial Ecology ; Microbiology ; Molecular Sequence Data ; Nature Conservation ; Nitrite Reductases - genetics ; Nitrites ; Paddy soils ; Phylogenetics ; Phylogeny ; Polymerase chain reaction ; Sedimentary soils ; Soil - analysis ; Soil composition ; SOIL MICROBIOLOGY ; Soil microorganisms ; Soil samples ; Soils ; Universities and colleges ; Urea ; Water Quality/Water Pollution
ispartofMicrobial ecology, 2010-11-01, Vol.60 (4), p.850-861
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descriptionThe effect of long-term fertilization on soildenitrifying communities was determined by measuring the abundance and diversity of the nitrite reductase genes nirK and nirS. Soil samples were collected from plots of a long-term fertilization experiment started in 1990, located in Taoyuan (110°72" E, 28°52" N), China. The treatments were no fertilizer (NF), urea (UR), balanced mineral fertilizers (BM), and BM combined with rice straw (BMR). The abundance, diversity, and composition of the soil-denitrifying bacteria were determined by using realtime quantitative PCR, terminal restriction fragment length polymorphism (T-RFLP), and cloning and sequencing of nirK and nirS genes. There was a pronounced difference in the community composition and diversity of nirK-containing denitrifiers responding to the long-term fertilization regimes; however, less variation was observed in communities of nirS-containing denitrifiers, indicating that denitrifiers possessing nirK were more sensitive to the fertilization practices than those with nirS. In contrast, fertilization regimes had similar effects on the copy numbers of nirK and nirS genes. The BMR treatment had the highest copy numbers of nirK and nirS, followed by the two mineral fertilization regimes (UR and BM), and the lowest was in the NF treatment. Of the measured soil parameters, the differences in the community composition of nirK and the abundance of nir denitrifiers were highly correlated with the soil carbon content. Therefore, longterm fertilization resulted in a strong impact on the community structure of nirK populations only, and total organic carbon was the dominant factor in relation to the variations of nir community sizes.
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1Bacteria - classification
2Bacteria - enzymology
3Bacteria - genetics
4Bacteria - isolation & purification
5Bacterial Proteins - genetics
6Biological and medical sciences
7Biomedical and Life Sciences
8Carbon content
9Ecology
10Fertilization
11Fertilizers
12Fertilizers - analysis
13Fundamental and applied biological sciences. Psychology
14Gene Dosage
15Geoecology/Natural Processes
16Life Sciences
17Microbial Ecology
18Microbiology
19Molecular Sequence Data
20Nature Conservation
21Nitrite Reductases - genetics
22Nitrites
23Paddy soils
24Phylogenetics
25Phylogeny
26Polymerase chain reaction
27Sedimentary soils
28Soil - analysis
29Soil composition
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32Soil samples
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35Urea
36Water Quality/Water Pollution
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titleImpact of Long-Term Fertilization on the Composition of Denitrifier Communities Based on Nitrite Reductase Analyses in a Paddy Soil
authorChen, Zhe ; Luo, Xiqian ; Hu, Ronggui ; Wu, Minna ; Wu, Jinshui ; Wei, Wenxue
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1Bacteria - classification
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abstractThe effect of long-term fertilization on soildenitrifying communities was determined by measuring the abundance and diversity of the nitrite reductase genes nirK and nirS. Soil samples were collected from plots of a long-term fertilization experiment started in 1990, located in Taoyuan (110°72" E, 28°52" N), China. The treatments were no fertilizer (NF), urea (UR), balanced mineral fertilizers (BM), and BM combined with rice straw (BMR). The abundance, diversity, and composition of the soil-denitrifying bacteria were determined by using realtime quantitative PCR, terminal restriction fragment length polymorphism (T-RFLP), and cloning and sequencing of nirK and nirS genes. There was a pronounced difference in the community composition and diversity of nirK-containing denitrifiers responding to the long-term fertilization regimes; however, less variation was observed in communities of nirS-containing denitrifiers, indicating that denitrifiers possessing nirK were more sensitive to the fertilization practices than those with nirS. In contrast, fertilization regimes had similar effects on the copy numbers of nirK and nirS genes. The BMR treatment had the highest copy numbers of nirK and nirS, followed by the two mineral fertilization regimes (UR and BM), and the lowest was in the NF treatment. Of the measured soil parameters, the differences in the community composition of nirK and the abundance of nir denitrifiers were highly correlated with the soil carbon content. Therefore, longterm fertilization resulted in a strong impact on the community structure of nirK populations only, and total organic carbon was the dominant factor in relation to the variations of nir community sizes.
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pubSpringer Science + Business Media, Inc
pmid20563573
doi10.1007/s00248-010-9700-z