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Sequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species

The genes coding for the outer surface protein A (OspA) of 19 different Borrelia burgdorferi strains belonging to the seven OspA-serotypes 1-7, previously described [Wilske et al. (1993) J Clin Microbiol, 31: 340-350], have been investigated. B. burgdorferi sensu lato strains were chosen from variou... Full description

Journal Title: Medical microbiology and immunology 1995, Vol.184 (2), p.73-80
Main Author: WILL, G
Other Authors: JAURIS-HEIPKE, S , SCHWAB, E , BUSCH, U , RÖSSLER, D , SOUTSCHEK, E , WILSKE, B , PREAC-MURSIC, V
Format: Electronic Article Electronic Article
Language: English
Subjects:
Amino Acid Sequence
Animals
Antigens, Surface - genetics
Bacterial Outer Membrane Proteins - genetics
Bacterial Vaccines
Bacteriology
Biological and medical sciences
Borrelia - classification
Borrelia - genetics
Borrelia afzelii
Borrelia burgdorferi
Borrelia burgdorferi Group - classification
Borrelia burgdorferi Group - genetics
Borrelia garinii
Cloning, Molecular
Electrophoresis, Gel, Pulsed-Field
Fundamental and applied biological sciences. Psychology
Genetics
Humans
Lipoproteins
Microbiology
Molecular Sequence Data
Publisher: Berlin: Springer
ID: ISSN: 0300-8584
Zum Text:
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title: Sequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species
format: Article
creator:
  • WILL, G
  • JAURIS-HEIPKE, S
  • SCHWAB, E
  • BUSCH, U
  • RÖSSLER, D
  • SOUTSCHEK, E
  • WILSKE, B
  • PREAC-MURSIC, V
subjects:
  • Amino Acid Sequence
  • Animals
  • Antigens, Surface - genetics
  • Bacterial Outer Membrane Proteins - genetics
  • Bacterial Vaccines
  • Bacteriology
  • Biological and medical sciences
  • Borrelia - classification
  • Borrelia - genetics
  • Borrelia afzelii
  • Borrelia burgdorferi
  • Borrelia burgdorferi Group - classification
  • Borrelia burgdorferi Group - genetics
  • Borrelia garinii
  • Cloning, Molecular
  • Electrophoresis, Gel, Pulsed-Field
  • Fundamental and applied biological sciences. Psychology
  • Genetics
  • Humans
  • Lipoproteins
  • Microbiology
  • Molecular Sequence Data
ispartof: Medical microbiology and immunology, 1995, Vol.184 (2), p.73-80
description: The genes coding for the outer surface protein A (OspA) of 19 different Borrelia burgdorferi strains belonging to the seven OspA-serotypes 1-7, previously described [Wilske et al. (1993) J Clin Microbiol, 31: 340-350], have been investigated. B. burgdorferi sensu lato strains were chosen from various biological sources (ticks, human skin and cerebrospinal fluid) as well as different geographical origins (Germany, Slovenia, Austria, United States). The open reading frames of all ospA genes consist of 819-825 nucleotides corresponding to proteins of approximately 30 kDa. The ospA sequences obtained in this study and previous published studies were compared with the results from OspA serotyping with monoclonal antibodies. The classification into the seven OspA serotypes could be confirmed on a genetic basis (ospA genotypes 1-7) for all strains analyzed so far (n = 29). In addition, one strain without OspA expression could be assigned to ospA genotype 2. Genetic stability could be proven for the ospA gene of B. burgdorferi strain PWudI after inocculation and reisolation from a gerbil. However, we found evidence for intragenic recombination by cluster analysis of ospA sequence data. Accordance of ospA genotype 1 strains with B. burgdorferi sensu stricto and ospA genotype 2 strains with B. afzelii, as well as the ospA genotype strains 3-7 with B. garinii was confirmed by pulsed-field gel electrophoresis of MluI-digested genomic DNA. B. garinii is not only more heterogeneous in respect to the OspA-encoding genes, but shows moreover major subgroups formed by genotypes 4, 5 and 6 and genotypes 3 and 7, respectively.
language: eng
source:
identifier: ISSN: 0300-8584
fulltext: no_fulltext
issn:
  • 0300-8584
  • 1432-1831
url: Link


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titleSequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species
creatorWILL, G ; JAURIS-HEIPKE, S ; SCHWAB, E ; BUSCH, U ; RÖSSLER, D ; SOUTSCHEK, E ; WILSKE, B ; PREAC-MURSIC, V
creatorcontribWILL, G ; JAURIS-HEIPKE, S ; SCHWAB, E ; BUSCH, U ; RÖSSLER, D ; SOUTSCHEK, E ; WILSKE, B ; PREAC-MURSIC, V
descriptionThe genes coding for the outer surface protein A (OspA) of 19 different Borrelia burgdorferi strains belonging to the seven OspA-serotypes 1-7, previously described [Wilske et al. (1993) J Clin Microbiol, 31: 340-350], have been investigated. B. burgdorferi sensu lato strains were chosen from various biological sources (ticks, human skin and cerebrospinal fluid) as well as different geographical origins (Germany, Slovenia, Austria, United States). The open reading frames of all ospA genes consist of 819-825 nucleotides corresponding to proteins of approximately 30 kDa. The ospA sequences obtained in this study and previous published studies were compared with the results from OspA serotyping with monoclonal antibodies. The classification into the seven OspA serotypes could be confirmed on a genetic basis (ospA genotypes 1-7) for all strains analyzed so far (n = 29). In addition, one strain without OspA expression could be assigned to ospA genotype 2. Genetic stability could be proven for the ospA gene of B. burgdorferi strain PWudI after inocculation and reisolation from a gerbil. However, we found evidence for intragenic recombination by cluster analysis of ospA sequence data. Accordance of ospA genotype 1 strains with B. burgdorferi sensu stricto and ospA genotype 2 strains with B. afzelii, as well as the ospA genotype strains 3-7 with B. garinii was confirmed by pulsed-field gel electrophoresis of MluI-digested genomic DNA. B. garinii is not only more heterogeneous in respect to the OspA-encoding genes, but shows moreover major subgroups formed by genotypes 4, 5 and 6 and genotypes 3 and 7, respectively.
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languageeng
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subjectAmino Acid Sequence ; Animals ; Antigens, Surface - genetics ; Bacterial Outer Membrane Proteins - genetics ; Bacterial Vaccines ; Bacteriology ; Biological and medical sciences ; Borrelia - classification ; Borrelia - genetics ; Borrelia afzelii ; Borrelia burgdorferi ; Borrelia burgdorferi Group - classification ; Borrelia burgdorferi Group - genetics ; Borrelia garinii ; Cloning, Molecular ; Electrophoresis, Gel, Pulsed-Field ; Fundamental and applied biological sciences. Psychology ; Genetics ; Humans ; Lipoproteins ; Microbiology ; Molecular Sequence Data
ispartofMedical microbiology and immunology, 1995, Vol.184 (2), p.73-80
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1JAURIS-HEIPKE, S
2SCHWAB, E
3BUSCH, U
4RÖSSLER, D
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7PREAC-MURSIC, V
title
0Sequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species
1Medical microbiology and immunology
addtitleMed Microbiol Immunol
descriptionThe genes coding for the outer surface protein A (OspA) of 19 different Borrelia burgdorferi strains belonging to the seven OspA-serotypes 1-7, previously described [Wilske et al. (1993) J Clin Microbiol, 31: 340-350], have been investigated. B. burgdorferi sensu lato strains were chosen from various biological sources (ticks, human skin and cerebrospinal fluid) as well as different geographical origins (Germany, Slovenia, Austria, United States). The open reading frames of all ospA genes consist of 819-825 nucleotides corresponding to proteins of approximately 30 kDa. The ospA sequences obtained in this study and previous published studies were compared with the results from OspA serotyping with monoclonal antibodies. The classification into the seven OspA serotypes could be confirmed on a genetic basis (ospA genotypes 1-7) for all strains analyzed so far (n = 29). In addition, one strain without OspA expression could be assigned to ospA genotype 2. Genetic stability could be proven for the ospA gene of B. burgdorferi strain PWudI after inocculation and reisolation from a gerbil. However, we found evidence for intragenic recombination by cluster analysis of ospA sequence data. Accordance of ospA genotype 1 strains with B. burgdorferi sensu stricto and ospA genotype 2 strains with B. afzelii, as well as the ospA genotype strains 3-7 with B. garinii was confirmed by pulsed-field gel electrophoresis of MluI-digested genomic DNA. B. garinii is not only more heterogeneous in respect to the OspA-encoding genes, but shows moreover major subgroups formed by genotypes 4, 5 and 6 and genotypes 3 and 7, respectively.
subject
0Amino Acid Sequence
1Animals
2Antigens, Surface - genetics
3Bacterial Outer Membrane Proteins - genetics
4Bacterial Vaccines
5Bacteriology
6Biological and medical sciences
7Borrelia - classification
8Borrelia - genetics
9Borrelia afzelii
10Borrelia burgdorferi
11Borrelia burgdorferi Group - classification
12Borrelia burgdorferi Group - genetics
13Borrelia garinii
14Cloning, Molecular
15Electrophoresis, Gel, Pulsed-Field
16Fundamental and applied biological sciences. Psychology
17Genetics
18Humans
19Lipoproteins
20Microbiology
21Molecular Sequence Data
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titleSequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species
authorWILL, G ; JAURIS-HEIPKE, S ; SCHWAB, E ; BUSCH, U ; RÖSSLER, D ; SOUTSCHEK, E ; WILSKE, B ; PREAC-MURSIC, V
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1Animals
2Antigens, Surface - genetics
3Bacterial Outer Membrane Proteins - genetics
4Bacterial Vaccines
5Bacteriology
6Biological and medical sciences
7Borrelia - classification
8Borrelia - genetics
9Borrelia afzelii
10Borrelia burgdorferi
11Borrelia burgdorferi Group - classification
12Borrelia burgdorferi Group - genetics
13Borrelia garinii
14Cloning, Molecular
15Electrophoresis, Gel, Pulsed-Field
16Fundamental and applied biological sciences. Psychology
17Genetics
18Humans
19Lipoproteins
20Microbiology
21Molecular Sequence Data
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atitleSequence analysis of ospA genes shows homogeneity within Borrelia burgdorferi sensu stricto and Borrelia afzelii strains but reveals major subgroups within the Borrelia garinii species
jtitleMedical microbiology and immunology
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abstractThe genes coding for the outer surface protein A (OspA) of 19 different Borrelia burgdorferi strains belonging to the seven OspA-serotypes 1-7, previously described [Wilske et al. (1993) J Clin Microbiol, 31: 340-350], have been investigated. B. burgdorferi sensu lato strains were chosen from various biological sources (ticks, human skin and cerebrospinal fluid) as well as different geographical origins (Germany, Slovenia, Austria, United States). The open reading frames of all ospA genes consist of 819-825 nucleotides corresponding to proteins of approximately 30 kDa. The ospA sequences obtained in this study and previous published studies were compared with the results from OspA serotyping with monoclonal antibodies. The classification into the seven OspA serotypes could be confirmed on a genetic basis (ospA genotypes 1-7) for all strains analyzed so far (n = 29). In addition, one strain without OspA expression could be assigned to ospA genotype 2. Genetic stability could be proven for the ospA gene of B. burgdorferi strain PWudI after inocculation and reisolation from a gerbil. However, we found evidence for intragenic recombination by cluster analysis of ospA sequence data. Accordance of ospA genotype 1 strains with B. burgdorferi sensu stricto and ospA genotype 2 strains with B. afzelii, as well as the ospA genotype strains 3-7 with B. garinii was confirmed by pulsed-field gel electrophoresis of MluI-digested genomic DNA. B. garinii is not only more heterogeneous in respect to the OspA-encoding genes, but shows moreover major subgroups formed by genotypes 4, 5 and 6 and genotypes 3 and 7, respectively.
copBerlin
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pmid7500914
doi10.1007/BF00221390
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