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Structure and properties of TentaGel resin beads: Implications for combinatorial library chemistry

In view of the widespread use of TentaGel resin beads for the synthesis of combinatorial libraries, the properties of TentaGel resin have been examined using a combination of confocal laser microscopy and NMR spectroscopy. Evidence is presented that trypsin, a 23.5-kDa enzyme, can penetrate to the c... Full description

Journal Title: Molecular diversity 1996-08, Vol.1 (4), p.223-232
Main Author: Quarrell, R
Other Authors: Claridge, T D , Weaver, G W , Lowe, G
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Netherlands
ID: ISSN: 1381-1991
Link: https://www.ncbi.nlm.nih.gov/pubmed/9237213
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title: Structure and properties of TentaGel resin beads: Implications for combinatorial library chemistry
format: Article
creator:
  • Quarrell, R
  • Claridge, T D
  • Weaver, G W
  • Lowe, G
subjects:
  • Deuterium
  • Directed Molecular Evolution - methods
  • Fluorescein-5-isothiocyanate
  • Magnetic Resonance Spectroscopy
  • Microscopy, Confocal
  • Molecular Structure
  • Particle Size
  • Polystyrenes - chemistry
  • Resins, Synthetic - chemistry
  • Solutions
  • Solvents
  • Trypsin
ispartof: Molecular diversity, 1996-08, Vol.1 (4), p.223-232
description: In view of the widespread use of TentaGel resin beads for the synthesis of combinatorial libraries, the properties of TentaGel resin have been examined using a combination of confocal laser microscopy and NMR spectroscopy. Evidence is presented that trypsin, a 23.5-kDa enzyme, can penetrate to the core of 90-microns TentaGel beads, and that the matrix of such beads permits molecular motion at a similar rate to that in solution. The beads act as a separate gel phase rather than as a porous solid. These conclusions have important implications for the bioassay of on-bead combinatorial chemical libraries.
language: eng
source:
identifier: ISSN: 1381-1991
fulltext: no_fulltext
issn:
  • 1381-1991
  • 1573-501X
url: Link


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titleStructure and properties of TentaGel resin beads: Implications for combinatorial library chemistry
creatorQuarrell, R ; Claridge, T D ; Weaver, G W ; Lowe, G
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descriptionIn view of the widespread use of TentaGel resin beads for the synthesis of combinatorial libraries, the properties of TentaGel resin have been examined using a combination of confocal laser microscopy and NMR spectroscopy. Evidence is presented that trypsin, a 23.5-kDa enzyme, can penetrate to the core of 90-microns TentaGel beads, and that the matrix of such beads permits molecular motion at a similar rate to that in solution. The beads act as a separate gel phase rather than as a porous solid. These conclusions have important implications for the bioassay of on-bead combinatorial chemical libraries.
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subjectDeuterium ; Directed Molecular Evolution - methods ; Fluorescein-5-isothiocyanate ; Magnetic Resonance Spectroscopy ; Microscopy, Confocal ; Molecular Structure ; Particle Size ; Polystyrenes - chemistry ; Resins, Synthetic - chemistry ; Solutions ; Solvents ; Trypsin
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descriptionIn view of the widespread use of TentaGel resin beads for the synthesis of combinatorial libraries, the properties of TentaGel resin have been examined using a combination of confocal laser microscopy and NMR spectroscopy. Evidence is presented that trypsin, a 23.5-kDa enzyme, can penetrate to the core of 90-microns TentaGel beads, and that the matrix of such beads permits molecular motion at a similar rate to that in solution. The beads act as a separate gel phase rather than as a porous solid. These conclusions have important implications for the bioassay of on-bead combinatorial chemical libraries.
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1Directed Molecular Evolution - methods
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7Polystyrenes - chemistry
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abstractIn view of the widespread use of TentaGel resin beads for the synthesis of combinatorial libraries, the properties of TentaGel resin have been examined using a combination of confocal laser microscopy and NMR spectroscopy. Evidence is presented that trypsin, a 23.5-kDa enzyme, can penetrate to the core of 90-microns TentaGel beads, and that the matrix of such beads permits molecular motion at a similar rate to that in solution. The beads act as a separate gel phase rather than as a porous solid. These conclusions have important implications for the bioassay of on-bead combinatorial chemical libraries.
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