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Immunochemical Purification and Characterization of a 74.0-kDa Schistosoma mansoni Antigen

A polypeptide antigen of 74.0 kDa molecular weight was detected in the antigenic extracts of the 3 developmental stages of Schistosoma mansoni (eggs, cercariae, and adult worms) by western blotting using BRL4 monoclonal antibody (mAb) that significantly protected mice at the levels of 51.6%, 42%, an... Full description

Journal Title: The Journal of parasitology 1998, Vol.84 (2), p.301-306
Main Author: ATTALLAH, A. M
Other Authors: EL MASRY, S. A , ISMAIL, H , ATTIA, H , MOHAMED ABDEL AZIZ , SHEHATTA, A. S , TABLL, A , SOLTAN, A , EL WASSIF, A
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Lawrence, KS: American Society of Parasitologists
ID: ISSN: 0022-3395
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recordid: cdi_proquest_miscellaneous_79840099
title: Immunochemical Purification and Characterization of a 74.0-kDa Schistosoma mansoni Antigen
format: Article
creator:
  • ATTALLAH, A. M
  • EL MASRY, S. A
  • ISMAIL, H
  • ATTIA, H
  • MOHAMED ABDEL AZIZ
  • SHEHATTA, A. S
  • TABLL, A
  • SOLTAN, A
  • EL WASSIF, A
subjects:
  • Adolescent
  • Adult
  • Aged
  • Amino acids
  • Amino Acids - analysis
  • Animals
  • Antibodies, Helminth - immunology
  • Antibodies, Monoclonal - immunology
  • Antigens
  • Antigens, Helminth - chemistry
  • Antigens, Helminth - isolation & purification
  • Antigens, Helminth - urine
  • Biochemistry. Physiology. Immunology. Molecular biology
  • Biological and medical sciences
  • Blotting, Western
  • Cercariae
  • Child
  • Child, Preschool
  • Chromatography, Affinity
  • Chromatography, High Pressure Liquid
  • Eggs
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Fundamental and applied biological sciences. Psychology
  • Gels
  • Humans
  • Hybridomas
  • Immunization, Passive
  • Immunology
  • Infections
  • Invertebrates
  • Mice
  • Mice, Inbred BALB C
  • Middle Aged
  • Nemathelminthia. Plathelmintha
  • Parasitology
  • Peptide Mapping
  • Physiology. Development
  • Schistosoma mansoni - immunology
  • Schistosomiasis
  • Schistosomiasis mansoni - immunology
  • Schistosomiasis mansoni - urine
  • Urine
ispartof: The Journal of parasitology, 1998, Vol.84 (2), p.301-306
description: A polypeptide antigen of 74.0 kDa molecular weight was detected in the antigenic extracts of the 3 developmental stages of Schistosoma mansoni (eggs, cercariae, and adult worms) by western blotting using BRL4 monoclonal antibody (mAb) that significantly protected mice at the levels of 51.6%, 42%, and 53.8% against challenge S. mansoni infection in 3 separate experiments. This antigen was isolated and purified from crude soluble worm antigen preparation by immunoaffinity chromatography using CNBr-activated sepharose-4B beads coupled with the BRL4 mAb. The purified antigen showed a single peak when analyzed by both high-performance liquid chromatography and high-performance capillary electrophoresis. The 74-kDa antigen was characterized as a protein in nature with 56.9% hydrophilic amino acids and 43.1% hydrophobic amino acids. This antigen was detected in 93% of urine samples from infected cases with specificity of 89% among noninfected cases using an enzyme immunoassay-fast dot-enzyme-linked immunosorbent assay based on BRL4 mAb.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0022-3395
fulltext: fulltext
issn:
  • 0022-3395
  • 1937-2345
url: Link


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titleImmunochemical Purification and Characterization of a 74.0-kDa Schistosoma mansoni Antigen
sourceAlma/SFX Local Collection
creatorATTALLAH, A. M ; EL MASRY, S. A ; ISMAIL, H ; ATTIA, H ; MOHAMED ABDEL AZIZ ; SHEHATTA, A. S ; TABLL, A ; SOLTAN, A ; EL WASSIF, A
creatorcontribATTALLAH, A. M ; EL MASRY, S. A ; ISMAIL, H ; ATTIA, H ; MOHAMED ABDEL AZIZ ; SHEHATTA, A. S ; TABLL, A ; SOLTAN, A ; EL WASSIF, A
descriptionA polypeptide antigen of 74.0 kDa molecular weight was detected in the antigenic extracts of the 3 developmental stages of Schistosoma mansoni (eggs, cercariae, and adult worms) by western blotting using BRL4 monoclonal antibody (mAb) that significantly protected mice at the levels of 51.6%, 42%, and 53.8% against challenge S. mansoni infection in 3 separate experiments. This antigen was isolated and purified from crude soluble worm antigen preparation by immunoaffinity chromatography using CNBr-activated sepharose-4B beads coupled with the BRL4 mAb. The purified antigen showed a single peak when analyzed by both high-performance liquid chromatography and high-performance capillary electrophoresis. The 74-kDa antigen was characterized as a protein in nature with 56.9% hydrophilic amino acids and 43.1% hydrophobic amino acids. This antigen was detected in 93% of urine samples from infected cases with specificity of 89% among noninfected cases using an enzyme immunoassay-fast dot-enzyme-linked immunosorbent assay based on BRL4 mAb.
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1EISSN: 1937-2345
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languageeng
publisherLawrence, KS: American Society of Parasitologists
subjectAdolescent ; Adult ; Aged ; Amino acids ; Amino Acids - analysis ; Animals ; Antibodies, Helminth - immunology ; Antibodies, Monoclonal - immunology ; Antigens ; Antigens, Helminth - chemistry ; Antigens, Helminth - isolation & purification ; Antigens, Helminth - urine ; Biochemistry. Physiology. Immunology. Molecular biology ; Biological and medical sciences ; Blotting, Western ; Cercariae ; Child ; Child, Preschool ; Chromatography, Affinity ; Chromatography, High Pressure Liquid ; Eggs ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Female ; Fundamental and applied biological sciences. Psychology ; Gels ; Humans ; Hybridomas ; Immunization, Passive ; Immunology ; Infections ; Invertebrates ; Mice ; Mice, Inbred BALB C ; Middle Aged ; Nemathelminthia. Plathelmintha ; Parasitology ; Peptide Mapping ; Physiology. Development ; Schistosoma mansoni - immunology ; Schistosomiasis ; Schistosomiasis mansoni - immunology ; Schistosomiasis mansoni - urine ; Urine
ispartofThe Journal of parasitology, 1998, Vol.84 (2), p.301-306
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6TABLL, A
7SOLTAN, A
8EL WASSIF, A
title
0Immunochemical Purification and Characterization of a 74.0-kDa Schistosoma mansoni Antigen
1The Journal of parasitology
addtitleJ Parasitol
descriptionA polypeptide antigen of 74.0 kDa molecular weight was detected in the antigenic extracts of the 3 developmental stages of Schistosoma mansoni (eggs, cercariae, and adult worms) by western blotting using BRL4 monoclonal antibody (mAb) that significantly protected mice at the levels of 51.6%, 42%, and 53.8% against challenge S. mansoni infection in 3 separate experiments. This antigen was isolated and purified from crude soluble worm antigen preparation by immunoaffinity chromatography using CNBr-activated sepharose-4B beads coupled with the BRL4 mAb. The purified antigen showed a single peak when analyzed by both high-performance liquid chromatography and high-performance capillary electrophoresis. The 74-kDa antigen was characterized as a protein in nature with 56.9% hydrophilic amino acids and 43.1% hydrophobic amino acids. This antigen was detected in 93% of urine samples from infected cases with specificity of 89% among noninfected cases using an enzyme immunoassay-fast dot-enzyme-linked immunosorbent assay based on BRL4 mAb.
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1Adult
2Aged
3Amino acids
4Amino Acids - analysis
5Animals
6Antibodies, Helminth - immunology
7Antibodies, Monoclonal - immunology
8Antigens
9Antigens, Helminth - chemistry
10Antigens, Helminth - isolation & purification
11Antigens, Helminth - urine
12Biochemistry. Physiology. Immunology. Molecular biology
13Biological and medical sciences
14Blotting, Western
15Cercariae
16Child
17Child, Preschool
18Chromatography, Affinity
19Chromatography, High Pressure Liquid
20Eggs
21Electrophoresis, Polyacrylamide Gel
22Enzyme-Linked Immunosorbent Assay
23Female
24Fundamental and applied biological sciences. Psychology
25Gels
26Humans
27Hybridomas
28Immunization, Passive
29Immunology
30Infections
31Invertebrates
32Mice
33Mice, Inbred BALB C
34Middle Aged
35Nemathelminthia. Plathelmintha
36Parasitology
37Peptide Mapping
38Physiology. Development
39Schistosoma mansoni - immunology
40Schistosomiasis
41Schistosomiasis mansoni - immunology
42Schistosomiasis mansoni - urine
43Urine
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titleImmunochemical Purification and Characterization of a 74.0-kDa Schistosoma mansoni Antigen
authorATTALLAH, A. M ; EL MASRY, S. A ; ISMAIL, H ; ATTIA, H ; MOHAMED ABDEL AZIZ ; SHEHATTA, A. S ; TABLL, A ; SOLTAN, A ; EL WASSIF, A
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34Middle Aged
35Nemathelminthia. Plathelmintha
36Parasitology
37Peptide Mapping
38Physiology. Development
39Schistosoma mansoni - immunology
40Schistosomiasis
41Schistosomiasis mansoni - immunology
42Schistosomiasis mansoni - urine
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7SOLTAN, A
8EL WASSIF, A
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1EL MASRY, S. A
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7SOLTAN, A
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jtitleThe Journal of parasitology
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date1998-04-01
risdate1998
volume84
issue2
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abstractA polypeptide antigen of 74.0 kDa molecular weight was detected in the antigenic extracts of the 3 developmental stages of Schistosoma mansoni (eggs, cercariae, and adult worms) by western blotting using BRL4 monoclonal antibody (mAb) that significantly protected mice at the levels of 51.6%, 42%, and 53.8% against challenge S. mansoni infection in 3 separate experiments. This antigen was isolated and purified from crude soluble worm antigen preparation by immunoaffinity chromatography using CNBr-activated sepharose-4B beads coupled with the BRL4 mAb. The purified antigen showed a single peak when analyzed by both high-performance liquid chromatography and high-performance capillary electrophoresis. The 74-kDa antigen was characterized as a protein in nature with 56.9% hydrophilic amino acids and 43.1% hydrophobic amino acids. This antigen was detected in 93% of urine samples from infected cases with specificity of 89% among noninfected cases using an enzyme immunoassay-fast dot-enzyme-linked immunosorbent assay based on BRL4 mAb.
copLawrence, KS
pubAmerican Society of Parasitologists
pmid9576502
doi10.2307/3284486