schliessen

Filtern

 

Bibliotheken

A TALE nuclease architecture for efficient genome editing

Nucleases that cleave unique genomic sequences in living cells can be used for targeted gene editing and mutagenesis. Here we develop a strategy for generating such reagents based on transcription activator-like effector (TALE) proteins from Xanthomonas. We identify TALE truncation variants that eff... Full description

Journal Title: Nature biotechnology 2011, Vol.29 (2), p.143-148
Main Author: Rebar, Edward J
Other Authors: Miller, Jeffrey C , Tan, Siyuan , Qiao, Guijuan , Barlow, Kyle A , Wang, Jianbin , Xia, Danny F , Meng, Xiangdong , Paschon, David E , Leung, Elo , Hinkley, Sarah J , Dulay, Gladys P , Hua, Kevin L , Ankoudinova, Irina , Cost, Gregory J , Urnov, Fyodor D , Zhang, H Steve , Holmes, Michael C , Zhang, Lei , Gregory, Philip D
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: New York, NY: Nature Publishing Group
ID: ISSN: 1087-0156
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_proquest_miscellaneous_874180339
title: A TALE nuclease architecture for efficient genome editing
format: Article
creator:
  • Rebar, Edward J
  • Miller, Jeffrey C
  • Tan, Siyuan
  • Qiao, Guijuan
  • Barlow, Kyle A
  • Wang, Jianbin
  • Xia, Danny F
  • Meng, Xiangdong
  • Paschon, David E
  • Leung, Elo
  • Hinkley, Sarah J
  • Dulay, Gladys P
  • Hua, Kevin L
  • Ankoudinova, Irina
  • Cost, Gregory J
  • Urnov, Fyodor D
  • Zhang, H Steve
  • Holmes, Michael C
  • Zhang, Lei
  • Gregory, Philip D
subjects:
  • Bacterial proteins
  • Bacterial Proteins - genetics
  • Bacterial Proteins - metabolism
  • Base Sequence
  • Binding Sites
  • Biological and medical sciences
  • Biotechnology
  • Combinatorial Chemistry Techniques - methods
  • Deoxyribonucleases, Type II Site-Specific - genetics
  • Deoxyribonucleases, Type II Site-Specific - metabolism
  • DNA - genetics
  • DNA - metabolism
  • Fundamental and applied biological sciences. Psychology
  • Gene expression
  • Genetic Engineering
  • Genome
  • Genomes
  • Genomics
  • Health aspects
  • Humans
  • K562 Cells
  • Methods
  • Molecular biology
  • Molecular Sequence Data
  • Mutagenesis
  • Mutagenesis, Site-Directed - methods
  • Nucleases
  • Physiological aspects
  • Receptors, CCR5 - genetics
  • Transcription factors
  • Transcription Factors - genetics
  • Transcription Factors - metabolism
  • Vascular Endothelial Growth Factor A - genetics
  • Xanthomonas
ispartof: Nature biotechnology, 2011, Vol.29 (2), p.143-148
description: Nucleases that cleave unique genomic sequences in living cells can be used for targeted gene editing and mutagenesis. Here we develop a strategy for generating such reagents based on transcription activator-like effector (TALE) proteins from Xanthomonas. We identify TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and use these nucleases to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%. We further show that designed TALEs can regulate endogenous mammalian genes. These studies demonstrate the effective application of designed TALE transcription factors and nucleases for the targeted regulation and modification of endogenous genes.
language: eng
source:
identifier: ISSN: 1087-0156
fulltext: no_fulltext
issn:
  • 1087-0156
  • 1546-1696
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.806821
LOCALfalse
PrimoNMBib
record
control
sourceidgale_proqu
recordidTN_cdi_proquest_miscellaneous_874180339
sourceformatXML
sourcesystemPC
galeidA249223343
sourcerecordidA249223343
originalsourceidFETCH-LOGICAL-c557t-1e806625fd31226300fa14c7bdebdcad03f00eb44422bdac4f775c4c06c1b010
addsrcrecordideNqN0u9r1DAYB_AiDjen4F8gRREn2PPJj6bty2NMNzgY6OHbkKZPuow23ZIU9L83x503T2RIoAnl8zxPG75Z9orAggCrP7k2LkhVlk-yE1JyURDRiKfpDHVVACnFcfY8hFsAEFyIZ9kxJaRqoCEnWbPM18vVRe5mPaAKmCuvb2xEHWePuZl8jsZYbdHFvEc3jZhjZ6N1_YvsyKgh4MvdfpqtP1-szy-L1fWXq_PlqtBlWcWCYA1C0NJ0jFAqGIBRhOuq7bDttOqAGQBsOeeUtp3S3FRVqbkGoUkLBE6z99u2d366nzFEOdqgcRiUw2kOsq44qYGxJsk3f8nbafYufZusSxAVI02d0Nst6tWA0jozRa_0pqVcUt5QyhhnSS3-odLqcLR6cmhsen9Q8OGgIJmIP2Kv5hDk1bev_2-vvx_aj3_Ydg7WYUiPYPubGLYlB_xsy7WfQvBo5J23o_I_JQG5SYpMSZGbpCT6endbcztit4e_o5HAux1QQavBeOW0DQ-O1Y2oCX24q_1MbaOKNv2VV3Z4ZLJTm5DtO-7BL5Be2oU
sourcetypeAggregation Database
isCDItrue
recordtypearticle
pqid850673198
display
typearticle
titleA TALE nuclease architecture for efficient genome editing
creatorRebar, Edward J ; Miller, Jeffrey C ; Tan, Siyuan ; Qiao, Guijuan ; Barlow, Kyle A ; Wang, Jianbin ; Xia, Danny F ; Meng, Xiangdong ; Paschon, David E ; Leung, Elo ; Hinkley, Sarah J ; Dulay, Gladys P ; Hua, Kevin L ; Ankoudinova, Irina ; Cost, Gregory J ; Urnov, Fyodor D ; Zhang, H Steve ; Holmes, Michael C ; Zhang, Lei ; Gregory, Philip D
creatorcontribRebar, Edward J ; Miller, Jeffrey C ; Tan, Siyuan ; Qiao, Guijuan ; Barlow, Kyle A ; Wang, Jianbin ; Xia, Danny F ; Meng, Xiangdong ; Paschon, David E ; Leung, Elo ; Hinkley, Sarah J ; Dulay, Gladys P ; Hua, Kevin L ; Ankoudinova, Irina ; Cost, Gregory J ; Urnov, Fyodor D ; Zhang, H Steve ; Holmes, Michael C ; Zhang, Lei ; Gregory, Philip D
descriptionNucleases that cleave unique genomic sequences in living cells can be used for targeted gene editing and mutagenesis. Here we develop a strategy for generating such reagents based on transcription activator-like effector (TALE) proteins from Xanthomonas. We identify TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and use these nucleases to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%. We further show that designed TALEs can regulate endogenous mammalian genes. These studies demonstrate the effective application of designed TALE transcription factors and nucleases for the targeted regulation and modification of endogenous genes.
identifier
0ISSN: 1087-0156
1EISSN: 1546-1696
2DOI: 10.1038/nbt.1755
3PMID: 21179091
4CODEN: NABIF9
languageeng
publisherNew York, NY: Nature Publishing Group
subjectBacterial proteins ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Base Sequence ; Binding Sites ; Biological and medical sciences ; Biotechnology ; Combinatorial Chemistry Techniques - methods ; Deoxyribonucleases, Type II Site-Specific - genetics ; Deoxyribonucleases, Type II Site-Specific - metabolism ; DNA - genetics ; DNA - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Genetic Engineering ; Genome ; Genomes ; Genomics ; Health aspects ; Humans ; K562 Cells ; Methods ; Molecular biology ; Molecular Sequence Data ; Mutagenesis ; Mutagenesis, Site-Directed - methods ; Nucleases ; Physiological aspects ; Receptors, CCR5 - genetics ; Transcription factors ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Vascular Endothelial Growth Factor A - genetics ; Xanthomonas
ispartofNature biotechnology, 2011, Vol.29 (2), p.143-148
rights
02015 INIST-CNRS
1COPYRIGHT 2011 Nature Publishing Group
2Copyright Nature Publishing Group Feb 2011
lds50peer_reviewed
citedbyFETCH-LOGICAL-c557t-1e806625fd31226300fa14c7bdebdcad03f00eb44422bdac4f775c4c06c1b010
citesFETCH-LOGICAL-c557t-1e806625fd31226300fa14c7bdebdcad03f00eb44422bdac4f775c4c06c1b010
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink
0$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23896812$$DView record in Pascal Francis
1$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21179091$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Rebar, Edward J
1Miller, Jeffrey C
2Tan, Siyuan
3Qiao, Guijuan
4Barlow, Kyle A
5Wang, Jianbin
6Xia, Danny F
7Meng, Xiangdong
8Paschon, David E
9Leung, Elo
10Hinkley, Sarah J
11Dulay, Gladys P
12Hua, Kevin L
13Ankoudinova, Irina
14Cost, Gregory J
15Urnov, Fyodor D
16Zhang, H Steve
17Holmes, Michael C
18Zhang, Lei
19Gregory, Philip D
title
0A TALE nuclease architecture for efficient genome editing
1Nature biotechnology
addtitleNat Biotechnol
descriptionNucleases that cleave unique genomic sequences in living cells can be used for targeted gene editing and mutagenesis. Here we develop a strategy for generating such reagents based on transcription activator-like effector (TALE) proteins from Xanthomonas. We identify TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and use these nucleases to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%. We further show that designed TALEs can regulate endogenous mammalian genes. These studies demonstrate the effective application of designed TALE transcription factors and nucleases for the targeted regulation and modification of endogenous genes.
subject
0Bacterial proteins
1Bacterial Proteins - genetics
2Bacterial Proteins - metabolism
3Base Sequence
4Binding Sites
5Biological and medical sciences
6Biotechnology
7Combinatorial Chemistry Techniques - methods
8Deoxyribonucleases, Type II Site-Specific - genetics
9Deoxyribonucleases, Type II Site-Specific - metabolism
10DNA - genetics
11DNA - metabolism
12Fundamental and applied biological sciences. Psychology
13Gene expression
14Genetic Engineering
15Genome
16Genomes
17Genomics
18Health aspects
19Humans
20K562 Cells
21Methods
22Molecular biology
23Molecular Sequence Data
24Mutagenesis
25Mutagenesis, Site-Directed - methods
26Nucleases
27Physiological aspects
28Receptors, CCR5 - genetics
29Transcription factors
30Transcription Factors - genetics
31Transcription Factors - metabolism
32Vascular Endothelial Growth Factor A - genetics
33Xanthomonas
issn
01087-0156
11546-1696
fulltextfalse
rsrctypearticle
creationdate2011
recordtypearticle
recordideNqN0u9r1DAYB_AiDjen4F8gRREn2PPJj6bty2NMNzgY6OHbkKZPuow23ZIU9L83x503T2RIoAnl8zxPG75Z9orAggCrP7k2LkhVlk-yE1JyURDRiKfpDHVVACnFcfY8hFsAEFyIZ9kxJaRqoCEnWbPM18vVRe5mPaAKmCuvb2xEHWePuZl8jsZYbdHFvEc3jZhjZ6N1_YvsyKgh4MvdfpqtP1-szy-L1fWXq_PlqtBlWcWCYA1C0NJ0jFAqGIBRhOuq7bDttOqAGQBsOeeUtp3S3FRVqbkGoUkLBE6z99u2d366nzFEOdqgcRiUw2kOsq44qYGxJsk3f8nbafYufZusSxAVI02d0Nst6tWA0jozRa_0pqVcUt5QyhhnSS3-odLqcLR6cmhsen9Q8OGgIJmIP2Kv5hDk1bev_2-vvx_aj3_Ydg7WYUiPYPubGLYlB_xsy7WfQvBo5J23o_I_JQG5SYpMSZGbpCT6endbcztit4e_o5HAux1QQavBeOW0DQ-O1Y2oCX24q_1MbaOKNv2VV3Z4ZLJTm5DtO-7BL5Be2oU
startdate201102
enddate201102
creator
0Rebar, Edward J
1Miller, Jeffrey C
2Tan, Siyuan
3Qiao, Guijuan
4Barlow, Kyle A
5Wang, Jianbin
6Xia, Danny F
7Meng, Xiangdong
8Paschon, David E
9Leung, Elo
10Hinkley, Sarah J
11Dulay, Gladys P
12Hua, Kevin L
13Ankoudinova, Irina
14Cost, Gregory J
15Urnov, Fyodor D
16Zhang, H Steve
17Holmes, Michael C
18Zhang, Lei
19Gregory, Philip D
generalNature Publishing Group
scope
0IQODW
1CGR
2CUY
3CVF
4ECM
5EIF
6NPM
7AAYXX
8CITATION
93V.
107QO
117QP
127QR
137T7
147TK
157TM
167X7
177XB
1888A
1988E
2088I
218AO
228FD
238FE
248FG
258FH
268FI
278FJ
288FK
298G5
30ABJCF
31ABUWG
32AZQEC
33BBNVY
34BENPR
35BGLVJ
36BHPHI
37C1K
38DWQXO
39FR3
40FYUFA
41GHDGH
42GNUQQ
43GUQSH
44HCIFZ
45K9.
46L6V
47LK8
48M0S
49M1P
50M2O
51M2P
52M7P
53M7S
54MBDVC
55P64
56PADUT
57PQEST
58PQQKQ
59PQUKI
60PRINS
61PTHSS
62Q9U
63RC3
sort
creationdate201102
titleA TALE nuclease architecture for efficient genome editing
authorRebar, Edward J ; Miller, Jeffrey C ; Tan, Siyuan ; Qiao, Guijuan ; Barlow, Kyle A ; Wang, Jianbin ; Xia, Danny F ; Meng, Xiangdong ; Paschon, David E ; Leung, Elo ; Hinkley, Sarah J ; Dulay, Gladys P ; Hua, Kevin L ; Ankoudinova, Irina ; Cost, Gregory J ; Urnov, Fyodor D ; Zhang, H Steve ; Holmes, Michael C ; Zhang, Lei ; Gregory, Philip D
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-c557t-1e806625fd31226300fa14c7bdebdcad03f00eb44422bdac4f775c4c06c1b010
rsrctypearticles
prefilterarticles
languageeng
creationdate2011
topic
0Bacterial proteins
1Bacterial Proteins - genetics
2Bacterial Proteins - metabolism
3Base Sequence
4Binding Sites
5Biological and medical sciences
6Biotechnology
7Combinatorial Chemistry Techniques - methods
8Deoxyribonucleases, Type II Site-Specific - genetics
9Deoxyribonucleases, Type II Site-Specific - metabolism
10DNA - genetics
11DNA - metabolism
12Fundamental and applied biological sciences. Psychology
13Gene expression
14Genetic Engineering
15Genome
16Genomes
17Genomics
18Health aspects
19Humans
20K562 Cells
21Methods
22Molecular biology
23Molecular Sequence Data
24Mutagenesis
25Mutagenesis, Site-Directed - methods
26Nucleases
27Physiological aspects
28Receptors, CCR5 - genetics
29Transcription factors
30Transcription Factors - genetics
31Transcription Factors - metabolism
32Vascular Endothelial Growth Factor A - genetics
33Xanthomonas
toplevelpeer_reviewed
creatorcontrib
0Rebar, Edward J
1Miller, Jeffrey C
2Tan, Siyuan
3Qiao, Guijuan
4Barlow, Kyle A
5Wang, Jianbin
6Xia, Danny F
7Meng, Xiangdong
8Paschon, David E
9Leung, Elo
10Hinkley, Sarah J
11Dulay, Gladys P
12Hua, Kevin L
13Ankoudinova, Irina
14Cost, Gregory J
15Urnov, Fyodor D
16Zhang, H Steve
17Holmes, Michael C
18Zhang, Lei
19Gregory, Philip D
collection
0Pascal-Francis
1Medline
2MEDLINE
3MEDLINE (Ovid)
4MEDLINE
5MEDLINE
6PubMed
7CrossRef
8ProQuest Central (Corporate)
9Biotechnology Research Abstracts
10Calcium & Calcified Tissue Abstracts
11Chemoreception Abstracts
12Industrial and Applied Microbiology Abstracts (Microbiology A)
13Neurosciences Abstracts
14Nucleic Acids Abstracts
15Health & Medical Collection
16ProQuest Central (purchase pre-March 2016)
17Biology Database (Alumni Edition)
18Medical Database (Alumni Edition)
19Science Database (Alumni Edition)
20ProQuest Pharma Collection
21Technology Research Database
22ProQuest SciTech Collection
23ProQuest Technology Collection
24ProQuest Natural Science Collection
25Hospital Premium Collection
26Hospital Premium Collection (Alumni Edition)
27ProQuest Central (Alumni) (purchase pre-March 2016)
28Research Library (Alumni Edition)
29Materials Science & Engineering Collection
30ProQuest Central (Alumni Edition)
31ProQuest Central Essentials
32Biological Science Collection
33ProQuest Central
34Technology Collection
35Natural Science Collection
36Environmental Sciences and Pollution Management
37ProQuest Central Korea
38Engineering Research Database
39Health Research Premium Collection
40Health Research Premium Collection (Alumni)
41ProQuest Central Student
42Research Library Prep
43SciTech Premium Collection
44ProQuest Health & Medical Complete (Alumni)
45ProQuest Engineering Collection
46ProQuest Biological Science Collection
47Health & Medical Collection (Alumni Edition)
48Medical Database
49Research Library
50Science Database
51Biological Science Database
52Engineering Database
53Research Library (Corporate)
54Biotechnology and BioEngineering Abstracts
55Research Library China
56ProQuest One Academic Eastern Edition
57ProQuest One Academic
58ProQuest One Academic UKI Edition
59ProQuest Central China
60Engineering Collection
61ProQuest Central Basic
62Genetics Abstracts
jtitleNature biotechnology
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Rebar, Edward J
1Miller, Jeffrey C
2Tan, Siyuan
3Qiao, Guijuan
4Barlow, Kyle A
5Wang, Jianbin
6Xia, Danny F
7Meng, Xiangdong
8Paschon, David E
9Leung, Elo
10Hinkley, Sarah J
11Dulay, Gladys P
12Hua, Kevin L
13Ankoudinova, Irina
14Cost, Gregory J
15Urnov, Fyodor D
16Zhang, H Steve
17Holmes, Michael C
18Zhang, Lei
19Gregory, Philip D
formatjournal
genrearticle
ristypeJOUR
atitleA TALE nuclease architecture for efficient genome editing
jtitleNature biotechnology
addtitleNat Biotechnol
date2011-02
risdate2011
volume29
issue2
spage143
epage148
pages143-148
issn1087-0156
eissn1546-1696
codenNABIF9
abstractNucleases that cleave unique genomic sequences in living cells can be used for targeted gene editing and mutagenesis. Here we develop a strategy for generating such reagents based on transcription activator-like effector (TALE) proteins from Xanthomonas. We identify TALE truncation variants that efficiently cleave DNA when linked to the catalytic domain of FokI and use these nucleases to generate discrete edits or small deletions within endogenous human NTF3 and CCR5 genes at efficiencies of up to 25%. We further show that designed TALEs can regulate endogenous mammalian genes. These studies demonstrate the effective application of designed TALE transcription factors and nucleases for the targeted regulation and modification of endogenous genes.
copNew York, NY
pubNature Publishing Group
pmid21179091
doi10.1038/nbt.1755
tpages8