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Genetic engineering of human pluripotent cells using TALE nucleases

Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator-like effector nucleases (TALENs) for five distinct genomic loci. At all... Full description

Journal Title: Nature biotechnology 2011, Vol.29 (8), p.731-734
Main Author: Jaenisch, Rudolf
Other Authors: Hockemeyer, Dirk , Wang, Haoyi , Kiani, Samira , Lai, Christine S , Gao, Qing , Cassady, John P , Cost, Gregory J , Zhang, Lei , Santiago, Yolanda , Miller, Jeffrey C , Zeitler, Bryan , Cherone, Jennifer M , Meng, Xiangdong , Hinkley, Sarah J , Rebar, Edward J , Gregory, Philip D , Urnov, Fyodor D
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: New York, NY: Nature Publishing Group
ID: ISSN: 1087-0156
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title: Genetic engineering of human pluripotent cells using TALE nucleases
format: Article
creator:
  • Jaenisch, Rudolf
  • Hockemeyer, Dirk
  • Wang, Haoyi
  • Kiani, Samira
  • Lai, Christine S
  • Gao, Qing
  • Cassady, John P
  • Cost, Gregory J
  • Zhang, Lei
  • Santiago, Yolanda
  • Miller, Jeffrey C
  • Zeitler, Bryan
  • Cherone, Jennifer M
  • Meng, Xiangdong
  • Hinkley, Sarah J
  • Rebar, Edward J
  • Gregory, Philip D
  • Urnov, Fyodor D
subjects:
  • Base Sequence
  • Biological and medical sciences
  • Biotechnology
  • Cellular biology
  • Data processing
  • Embryonic Stem Cells - physiology
  • Endonucleases - genetics
  • Endonucleases - metabolism
  • Enzymes
  • Fundamental and applied biological sciences. Psychology
  • Gene Targeting - methods
  • Genetic engineering
  • Genetic Engineering - methods
  • Genetic technics
  • Genomics
  • Homeodomain Proteins - genetics
  • Humans
  • Induced Pluripotent Stem Cells - physiology
  • Methods. Procedures. Technologies
  • Molecular Sequence Data
  • Myosin-Light-Chain Phosphatase - genetics
  • Nucleases
  • Octamer Transcription Factor-3 - genetics
  • Physiological aspects
  • Stem cells
  • Transcription Factors - genetics
  • Transcription Factors - metabolism
  • Zinc finger proteins
  • Zinc Fingers
ispartof: Nature biotechnology, 2011, Vol.29 (8), p.731-734
description: Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator-like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
language: eng
source:
identifier: ISSN: 1087-0156
fulltext: no_fulltext
issn:
  • 1087-0156
  • 1546-1696
url: Link


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titleGenetic engineering of human pluripotent cells using TALE nucleases
creatorJaenisch, Rudolf ; Hockemeyer, Dirk ; Wang, Haoyi ; Kiani, Samira ; Lai, Christine S ; Gao, Qing ; Cassady, John P ; Cost, Gregory J ; Zhang, Lei ; Santiago, Yolanda ; Miller, Jeffrey C ; Zeitler, Bryan ; Cherone, Jennifer M ; Meng, Xiangdong ; Hinkley, Sarah J ; Rebar, Edward J ; Gregory, Philip D ; Urnov, Fyodor D
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descriptionTargeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator-like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
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subjectBase Sequence ; Biological and medical sciences ; Biotechnology ; Cellular biology ; Data processing ; Embryonic Stem Cells - physiology ; Endonucleases - genetics ; Endonucleases - metabolism ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Gene Targeting - methods ; Genetic engineering ; Genetic Engineering - methods ; Genetic technics ; Genomics ; Homeodomain Proteins - genetics ; Humans ; Induced Pluripotent Stem Cells - physiology ; Methods. Procedures. Technologies ; Molecular Sequence Data ; Myosin-Light-Chain Phosphatase - genetics ; Nucleases ; Octamer Transcription Factor-3 - genetics ; Physiological aspects ; Stem cells ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Zinc finger proteins ; Zinc Fingers
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descriptionTargeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator-like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
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titleGenetic engineering of human pluripotent cells using TALE nucleases
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1Biological and medical sciences
2Biotechnology
3Cellular biology
4Data processing
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6Endonucleases - genetics
7Endonucleases - metabolism
8Enzymes
9Fundamental and applied biological sciences. Psychology
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12Genetic Engineering - methods
13Genetic technics
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15Homeodomain Proteins - genetics
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17Induced Pluripotent Stem Cells - physiology
18Methods. Procedures. Technologies
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21Nucleases
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23Physiological aspects
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abstractTargeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator-like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
copNew York, NY
pubNature Publishing Group
pmid21738127
doi10.1038/nbt.1927
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