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Improved performance with saliva and urine as alternative DNA sources for malaria diagnosis by mitochondrial DNA-based PCR assays

Saliva and urine from malaria-infected individuals contain trace amounts of Plasmodium DNA, and therefore, could be used as alternative specimens for diagnosis. A nested PCR targeting the mitochondrial cytochrome b gene (Cytb-PCR) of four human malaria species and Plasmodium knowlesi was developed a... Full description

Journal Title: Clinical Microbiology and Infection 2011, Vol.17 (10), p.1484-1491
Main Author: Putaporntip, C
Other Authors: Buppan, P , Jongwutiwes, S
Format: Electronic Article Electronic Article
Language: English
Subjects:
DNA
RNA
Quelle: Alma/SFX Local Collection
Publisher: Oxford, UK: Elsevier Ltd
ID: ISSN: 1198-743X
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title: Improved performance with saliva and urine as alternative DNA sources for malaria diagnosis by mitochondrial DNA-based PCR assays
format: Article
creator:
  • Putaporntip, C
  • Buppan, P
  • Jongwutiwes, S
subjects:
  • Adolescent
  • Adult
  • Aged
  • Analysis
  • Biological and medical sciences
  • Child
  • Child, Preschool
  • Cytochrome b
  • Cytochromes b - genetics
  • Diagnosis
  • DNA
  • DNA, Mitochondrial - genetics
  • DNA, Protozoan - blood
  • DNA, Protozoan - genetics
  • DNA, Protozoan - urine
  • Female
  • Genetic research
  • Human protozoal diseases
  • Humans
  • Infectious Diseases
  • Malaria
  • Malaria - blood
  • Malaria - diagnosis
  • Malaria - parasitology
  • Malaria - urine
  • Male
  • Medical sciences
  • Microbiology (medical)
  • Microscopy
  • Middle Aged
  • Mitochondria - genetics
  • mitochondrial cytochrome b
  • mitochondrial cytochrome b
  • nested PCR
  • Parasitic diseases
  • Plasmodium - genetics
  • Plasmodium - isolation & purification
  • Plasmodium - pathogenicity
  • Plasmodium falciparum
  • Plasmodium knowlesi
  • Polymerase Chain Reaction - methods
  • Protozoal diseases
  • RNA
  • RNA, Ribosomal, 18S - genetics
  • saliva
  • Saliva - parasitology
  • saliva, urine
  • Sensitivity and Specificity
  • urine
  • Young Adult
ispartof: Clinical Microbiology and Infection, 2011, Vol.17 (10), p.1484-1491
description: Saliva and urine from malaria-infected individuals contain trace amounts of Plasmodium DNA, and therefore, could be used as alternative specimens for diagnosis. A nested PCR targeting the mitochondrial cytochrome b gene (Cytb-PCR) of four human malaria species and Plasmodium knowlesi was developed and tested with 693 blood samples from febrile patients living in diverse malaria-endemic areas of Thailand, and compared with microscopy and nested PCR targeting small-subunit rRNA (18S-PCR). Cytb-PCR was 16% and 39.8% more sensitive than 18S-PCR and microscopy, respectively, in detecting all of these malarial species in blood samples. Importantly, 34% and 17% of Plasmodium falciparum and Plasmodium vivax mono-infections, respectively, detected by microscopy were, in fact, mixed P. falciparum and P. non-falciparum infections. Analysis of matched blood, saliva and urine from 157 individuals showed that microscopy and Cytb-PCR of saliva yielded no significant difference in detecting P. falciparum and P. vivax. However, Cytb-PCR of saliva was more sensitive than microscopy for diagnosis of mixed-species infections. A combination of Cytb-PCR of saliva and of urine significantly outperformed microscopy (p 0.0098 for P. falciparum, p 0.006 for P. vivax, and p 0.0002 for mixed infections). Furthermore, Plasmodium malariae and P. knowlesi could also be identified in saliva and urine with this method. Therefore, the Cytb-PCR developed herein offers a high potential for the use of both saliva and urine for malaria diagnosis, with a sensitivity comparable with or superior to that of microscopy.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 1198-743X
fulltext: fulltext
issn:
  • 1198-743X
  • 1469-0691
url: Link


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descriptionSaliva and urine from malaria-infected individuals contain trace amounts of Plasmodium DNA, and therefore, could be used as alternative specimens for diagnosis. A nested PCR targeting the mitochondrial cytochrome b gene (Cytb-PCR) of four human malaria species and Plasmodium knowlesi was developed and tested with 693 blood samples from febrile patients living in diverse malaria-endemic areas of Thailand, and compared with microscopy and nested PCR targeting small-subunit rRNA (18S-PCR). Cytb-PCR was 16% and 39.8% more sensitive than 18S-PCR and microscopy, respectively, in detecting all of these malarial species in blood samples. Importantly, 34% and 17% of Plasmodium falciparum and Plasmodium vivax mono-infections, respectively, detected by microscopy were, in fact, mixed P. falciparum and P. non-falciparum infections. Analysis of matched blood, saliva and urine from 157 individuals showed that microscopy and Cytb-PCR of saliva yielded no significant difference in detecting P. falciparum and P. vivax. However, Cytb-PCR of saliva was more sensitive than microscopy for diagnosis of mixed-species infections. A combination of Cytb-PCR of saliva and of urine significantly outperformed microscopy (p 0.0098 for P. falciparum, p 0.006 for P. vivax, and p 0.0002 for mixed infections). Furthermore, Plasmodium malariae and P. knowlesi could also be identified in saliva and urine with this method. Therefore, the Cytb-PCR developed herein offers a high potential for the use of both saliva and urine for malaria diagnosis, with a sensitivity comparable with or superior to that of microscopy.
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subjectAdolescent ; Adult ; Aged ; Analysis ; Biological and medical sciences ; Child ; Child, Preschool ; Cytochrome b ; Cytochromes b - genetics ; Diagnosis ; DNA ; DNA, Mitochondrial - genetics ; DNA, Protozoan - blood ; DNA, Protozoan - genetics ; DNA, Protozoan - urine ; Female ; Genetic research ; Human protozoal diseases ; Humans ; Infectious Diseases ; Malaria ; Malaria - blood ; Malaria - diagnosis ; Malaria - parasitology ; Malaria - urine ; Male ; Medical sciences ; Microbiology (medical) ; Microscopy ; Middle Aged ; Mitochondria - genetics ; mitochondrial cytochrome b ; mitochondrial cytochrome b ; nested PCR ; Parasitic diseases ; Plasmodium - genetics ; Plasmodium - isolation & purification ; Plasmodium - pathogenicity ; Plasmodium falciparum ; Plasmodium knowlesi ; Polymerase Chain Reaction - methods ; Protozoal diseases ; RNA ; RNA, Ribosomal, 18S - genetics ; saliva ; Saliva - parasitology ; saliva, urine ; Sensitivity and Specificity ; urine ; Young Adult
ispartofClinical Microbiology and Infection, 2011, Vol.17 (10), p.1484-1491
rights
02011 European Society of Clinical Infectious Diseases
12011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases
22015 INIST-CNRS
32011 The Authors. Clinical Microbiology and Infection © 2011 European Society of Clinical Microbiology and Infectious Diseases.
4COPYRIGHT 2011 Blackwell Publishers Ltd.
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descriptionSaliva and urine from malaria-infected individuals contain trace amounts of Plasmodium DNA, and therefore, could be used as alternative specimens for diagnosis. A nested PCR targeting the mitochondrial cytochrome b gene (Cytb-PCR) of four human malaria species and Plasmodium knowlesi was developed and tested with 693 blood samples from febrile patients living in diverse malaria-endemic areas of Thailand, and compared with microscopy and nested PCR targeting small-subunit rRNA (18S-PCR). Cytb-PCR was 16% and 39.8% more sensitive than 18S-PCR and microscopy, respectively, in detecting all of these malarial species in blood samples. Importantly, 34% and 17% of Plasmodium falciparum and Plasmodium vivax mono-infections, respectively, detected by microscopy were, in fact, mixed P. falciparum and P. non-falciparum infections. Analysis of matched blood, saliva and urine from 157 individuals showed that microscopy and Cytb-PCR of saliva yielded no significant difference in detecting P. falciparum and P. vivax. However, Cytb-PCR of saliva was more sensitive than microscopy for diagnosis of mixed-species infections. A combination of Cytb-PCR of saliva and of urine significantly outperformed microscopy (p 0.0098 for P. falciparum, p 0.006 for P. vivax, and p 0.0002 for mixed infections). Furthermore, Plasmodium malariae and P. knowlesi could also be identified in saliva and urine with this method. Therefore, the Cytb-PCR developed herein offers a high potential for the use of both saliva and urine for malaria diagnosis, with a sensitivity comparable with or superior to that of microscopy.
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39Plasmodium knowlesi
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abstractSaliva and urine from malaria-infected individuals contain trace amounts of Plasmodium DNA, and therefore, could be used as alternative specimens for diagnosis. A nested PCR targeting the mitochondrial cytochrome b gene (Cytb-PCR) of four human malaria species and Plasmodium knowlesi was developed and tested with 693 blood samples from febrile patients living in diverse malaria-endemic areas of Thailand, and compared with microscopy and nested PCR targeting small-subunit rRNA (18S-PCR). Cytb-PCR was 16% and 39.8% more sensitive than 18S-PCR and microscopy, respectively, in detecting all of these malarial species in blood samples. Importantly, 34% and 17% of Plasmodium falciparum and Plasmodium vivax mono-infections, respectively, detected by microscopy were, in fact, mixed P. falciparum and P. non-falciparum infections. Analysis of matched blood, saliva and urine from 157 individuals showed that microscopy and Cytb-PCR of saliva yielded no significant difference in detecting P. falciparum and P. vivax. However, Cytb-PCR of saliva was more sensitive than microscopy for diagnosis of mixed-species infections. A combination of Cytb-PCR of saliva and of urine significantly outperformed microscopy (p 0.0098 for P. falciparum, p 0.006 for P. vivax, and p 0.0002 for mixed infections). Furthermore, Plasmodium malariae and P. knowlesi could also be identified in saliva and urine with this method. Therefore, the Cytb-PCR developed herein offers a high potential for the use of both saliva and urine for malaria diagnosis, with a sensitivity comparable with or superior to that of microscopy.
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