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Subtype-specific neuronal differentiation of PC12 cells transfected with alpha2-adrenergic receptors

Cells of the PC12 rat pheochromocytoma cell line acquire characteristics of sympathetic neurons under appropriate treatment. Stably transfected PC12 cells expressing individual alpha2-adrenergic receptor (alpha2-AR) subtypes were used to assess the role of alpha2-ARs in neuronal differentiation and... Full description

Journal Title: European journal of cell biology 2002-06, Vol.81 (6), p.363
Main Author: Taraviras, Stavros
Other Authors: Olli-Lähdesmäki, Tuire , Lymperopoulos, Anastasios , Charitonidou, Despina , Mavroidis, Manolis , Kallio, Jaana , Scheinin, Mika , Flordellis, Christodoulos
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Germany
ID: ISSN: 0171-9335
Link: https://www.ncbi.nlm.nih.gov/pubmed/12113477
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recordid: cdi_pubmed_primary_12113477
title: Subtype-specific neuronal differentiation of PC12 cells transfected with alpha2-adrenergic receptors
format: Article
creator:
  • Taraviras, Stavros
  • Olli-Lähdesmäki, Tuire
  • Lymperopoulos, Anastasios
  • Charitonidou, Despina
  • Mavroidis, Manolis
  • Kallio, Jaana
  • Scheinin, Mika
  • Flordellis, Christodoulos
subjects:
  • Adrenergic alpha-2 Receptor Agonists
  • Adrenergic alpha-Antagonists - pharmacology
  • Animals
  • Cell Differentiation - drug effects
  • Cell Differentiation - physiology
  • Cell Membrane - metabolism
  • Cell Size - drug effects
  • Cell Size - genetics
  • Central Nervous System - embryology
  • Central Nervous System - growth & development
  • Central Nervous System - metabolism
  • Drug Interactions - physiology
  • Enzyme Inhibitors - pharmacology
  • Epinephrine
  • Genetic Vectors
  • Immunohistochemistry
  • MAP Kinase Signaling System - drug effects
  • MAP Kinase Signaling System - physiology
  • Mitogen-Activated Protein Kinases - drug effects
  • Mitogen-Activated Protein Kinases - metabolism
  • Nerve Growth Factor - pharmacology
  • Neurites - drug effects
  • Neurites - metabolism
  • Neurites - ultrastructure
  • Neurons - cytology
  • Neurons - drug effects
  • Neurons - metabolism
  • PC12 Cells
  • Phosphatidylinositol 3-Kinases - drug effects
  • Phosphatidylinositol 3-Kinases - metabolism
  • Phosphorylation - drug effects
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins - drug effects
  • Proto-Oncogene Proteins - metabolism
  • Proto-Oncogene Proteins c-akt
  • Rats
  • Receptors, Adrenergic, alpha-2 - genetics
  • Receptors, Adrenergic, alpha-2 - metabolism
  • Transcription Factor AP-1 - drug effects
  • Transcription Factor AP-1 - metabolism
  • Transfection
ispartof: European journal of cell biology, 2002-06, Vol.81 (6), p.363
description: Cells of the PC12 rat pheochromocytoma cell line acquire characteristics of sympathetic neurons under appropriate treatment. Stably transfected PC12 cells expressing individual alpha2-adrenergic receptor (alpha2-AR) subtypes were used to assess the role of alpha2-ARs in neuronal differentiation and to characterise the signalling pathways activated by the alpha2-AR agonist epinephrine in these cells. The effects of alpha2-AR activation were compared with the differentiating action and the signalling mechanisms of nerve growth factor (NGF). Epinephrine induced neuronal differentiation of PC12alpha2 cells through alpha2-AR activation in a subtype-dependent manner, internalization of all human alpha2-AR subtypes, and activation of mitogen-activated protein kinase (MAPK) and the serine-threonine protein kinase Akt. Epinephrine and NGF showed synergism in their differentiating effects. The MAPK kinase (MEK-1) inhibitor PD 98059 abolished the differentiating effect of epinephrine indicating that the differentiation is dependent on MAPK activation. Activating protein-1 (AP-1) DNA-binding activity was increased after epinephrine treatment in all three PC12alpha2 subtype clones. Evaluation of the potential physiological consequences of these findings requires further studies on endogenously expressed alpha2-ARs in neuronal cells.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0171-9335
fulltext: fulltext
issn:
  • 0171-9335
  • 1618-1298
url: Link


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titleSubtype-specific neuronal differentiation of PC12 cells transfected with alpha2-adrenergic receptors
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creatorTaraviras, Stavros ; Olli-Lähdesmäki, Tuire ; Lymperopoulos, Anastasios ; Charitonidou, Despina ; Mavroidis, Manolis ; Kallio, Jaana ; Scheinin, Mika ; Flordellis, Christodoulos
creatorcontribTaraviras, Stavros ; Olli-Lähdesmäki, Tuire ; Lymperopoulos, Anastasios ; Charitonidou, Despina ; Mavroidis, Manolis ; Kallio, Jaana ; Scheinin, Mika ; Flordellis, Christodoulos
descriptionCells of the PC12 rat pheochromocytoma cell line acquire characteristics of sympathetic neurons under appropriate treatment. Stably transfected PC12 cells expressing individual alpha2-adrenergic receptor (alpha2-AR) subtypes were used to assess the role of alpha2-ARs in neuronal differentiation and to characterise the signalling pathways activated by the alpha2-AR agonist epinephrine in these cells. The effects of alpha2-AR activation were compared with the differentiating action and the signalling mechanisms of nerve growth factor (NGF). Epinephrine induced neuronal differentiation of PC12alpha2 cells through alpha2-AR activation in a subtype-dependent manner, internalization of all human alpha2-AR subtypes, and activation of mitogen-activated protein kinase (MAPK) and the serine-threonine protein kinase Akt. Epinephrine and NGF showed synergism in their differentiating effects. The MAPK kinase (MEK-1) inhibitor PD 98059 abolished the differentiating effect of epinephrine indicating that the differentiation is dependent on MAPK activation. Activating protein-1 (AP-1) DNA-binding activity was increased after epinephrine treatment in all three PC12alpha2 subtype clones. Evaluation of the potential physiological consequences of these findings requires further studies on endogenously expressed alpha2-ARs in neuronal cells.
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subjectAdrenergic alpha-2 Receptor Agonists ; Adrenergic alpha-Antagonists - pharmacology ; Animals ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell Membrane - metabolism ; Cell Size - drug effects ; Cell Size - genetics ; Central Nervous System - embryology ; Central Nervous System - growth & development ; Central Nervous System - metabolism ; Drug Interactions - physiology ; Enzyme Inhibitors - pharmacology ; Epinephrine ; Genetic Vectors ; Immunohistochemistry ; MAP Kinase Signaling System - drug effects ; MAP Kinase Signaling System - physiology ; Mitogen-Activated Protein Kinases - drug effects ; Mitogen-Activated Protein Kinases - metabolism ; Nerve Growth Factor - pharmacology ; Neurites - drug effects ; Neurites - metabolism ; Neurites - ultrastructure ; Neurons - cytology ; Neurons - drug effects ; Neurons - metabolism ; PC12 Cells ; Phosphatidylinositol 3-Kinases - drug effects ; Phosphatidylinositol 3-Kinases - metabolism ; Phosphorylation - drug effects ; Protein-Serine-Threonine Kinases ; Proto-Oncogene Proteins - drug effects ; Proto-Oncogene Proteins - metabolism ; Proto-Oncogene Proteins c-akt ; Rats ; Receptors, Adrenergic, alpha-2 - genetics ; Receptors, Adrenergic, alpha-2 - metabolism ; Transcription Factor AP-1 - drug effects ; Transcription Factor AP-1 - metabolism ; Transfection
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6Scheinin, Mika
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0Subtype-specific neuronal differentiation of PC12 cells transfected with alpha2-adrenergic receptors
1European journal of cell biology
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descriptionCells of the PC12 rat pheochromocytoma cell line acquire characteristics of sympathetic neurons under appropriate treatment. Stably transfected PC12 cells expressing individual alpha2-adrenergic receptor (alpha2-AR) subtypes were used to assess the role of alpha2-ARs in neuronal differentiation and to characterise the signalling pathways activated by the alpha2-AR agonist epinephrine in these cells. The effects of alpha2-AR activation were compared with the differentiating action and the signalling mechanisms of nerve growth factor (NGF). Epinephrine induced neuronal differentiation of PC12alpha2 cells through alpha2-AR activation in a subtype-dependent manner, internalization of all human alpha2-AR subtypes, and activation of mitogen-activated protein kinase (MAPK) and the serine-threonine protein kinase Akt. Epinephrine and NGF showed synergism in their differentiating effects. The MAPK kinase (MEK-1) inhibitor PD 98059 abolished the differentiating effect of epinephrine indicating that the differentiation is dependent on MAPK activation. Activating protein-1 (AP-1) DNA-binding activity was increased after epinephrine treatment in all three PC12alpha2 subtype clones. Evaluation of the potential physiological consequences of these findings requires further studies on endogenously expressed alpha2-ARs in neuronal cells.
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0Adrenergic alpha-2 Receptor Agonists
1Adrenergic alpha-Antagonists - pharmacology
2Animals
3Cell Differentiation - drug effects
4Cell Differentiation - physiology
5Cell Membrane - metabolism
6Cell Size - drug effects
7Cell Size - genetics
8Central Nervous System - embryology
9Central Nervous System - growth & development
10Central Nervous System - metabolism
11Drug Interactions - physiology
12Enzyme Inhibitors - pharmacology
13Epinephrine
14Genetic Vectors
15Immunohistochemistry
16MAP Kinase Signaling System - drug effects
17MAP Kinase Signaling System - physiology
18Mitogen-Activated Protein Kinases - drug effects
19Mitogen-Activated Protein Kinases - metabolism
20Nerve Growth Factor - pharmacology
21Neurites - drug effects
22Neurites - metabolism
23Neurites - ultrastructure
24Neurons - cytology
25Neurons - drug effects
26Neurons - metabolism
27PC12 Cells
28Phosphatidylinositol 3-Kinases - drug effects
29Phosphatidylinositol 3-Kinases - metabolism
30Phosphorylation - drug effects
31Protein-Serine-Threonine Kinases
32Proto-Oncogene Proteins - drug effects
33Proto-Oncogene Proteins - metabolism
34Proto-Oncogene Proteins c-akt
35Rats
36Receptors, Adrenergic, alpha-2 - genetics
37Receptors, Adrenergic, alpha-2 - metabolism
38Transcription Factor AP-1 - drug effects
39Transcription Factor AP-1 - metabolism
40Transfection
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titleSubtype-specific neuronal differentiation of PC12 cells transfected with alpha2-adrenergic receptors
authorTaraviras, Stavros ; Olli-Lähdesmäki, Tuire ; Lymperopoulos, Anastasios ; Charitonidou, Despina ; Mavroidis, Manolis ; Kallio, Jaana ; Scheinin, Mika ; Flordellis, Christodoulos
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12Enzyme Inhibitors - pharmacology
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19Mitogen-Activated Protein Kinases - metabolism
20Nerve Growth Factor - pharmacology
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31Protein-Serine-Threonine Kinases
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33Proto-Oncogene Proteins - metabolism
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35Rats
36Receptors, Adrenergic, alpha-2 - genetics
37Receptors, Adrenergic, alpha-2 - metabolism
38Transcription Factor AP-1 - drug effects
39Transcription Factor AP-1 - metabolism
40Transfection
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6Scheinin, Mika
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abstractCells of the PC12 rat pheochromocytoma cell line acquire characteristics of sympathetic neurons under appropriate treatment. Stably transfected PC12 cells expressing individual alpha2-adrenergic receptor (alpha2-AR) subtypes were used to assess the role of alpha2-ARs in neuronal differentiation and to characterise the signalling pathways activated by the alpha2-AR agonist epinephrine in these cells. The effects of alpha2-AR activation were compared with the differentiating action and the signalling mechanisms of nerve growth factor (NGF). Epinephrine induced neuronal differentiation of PC12alpha2 cells through alpha2-AR activation in a subtype-dependent manner, internalization of all human alpha2-AR subtypes, and activation of mitogen-activated protein kinase (MAPK) and the serine-threonine protein kinase Akt. Epinephrine and NGF showed synergism in their differentiating effects. The MAPK kinase (MEK-1) inhibitor PD 98059 abolished the differentiating effect of epinephrine indicating that the differentiation is dependent on MAPK activation. Activating protein-1 (AP-1) DNA-binding activity was increased after epinephrine treatment in all three PC12alpha2 subtype clones. Evaluation of the potential physiological consequences of these findings requires further studies on endogenously expressed alpha2-ARs in neuronal cells.
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