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In vitro study of nano-HA/PLLA composite scaffold for rabbit BMSC differentiation under TGF-β1 induction

The aim of this study is to investigate the effects of differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs) into chondrocytes induced by transforming growth factor-betal (TCP-β1) composite poly-lactide-co-glycolic acid/nano-hydroxyapatite (PLLA/nano-HA) to the construction of biomim... Full description

Journal Title: In vitro cellular & developmental biology. Animal 2014-03-01, Vol.50 (3), p.214-220
Main Author: Zhu, Weimin
Other Authors: Chen, Kang , Lu, Wei , Sun, Qifeng , Peng, Liangquan , Fen, Wenzhe , Li, Hao , Ou, Yangkan , Liu, Haifeng , Wang, Daping , Zeng, Yanjun
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Boston: Springer Science + Business Media
ID: ISSN: 1071-2690
Link: https://www.ncbi.nlm.nih.gov/pubmed/24368571
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recordid: cdi_pubmed_primary_24368571
title: In vitro study of nano-HA/PLLA composite scaffold for rabbit BMSC differentiation under TGF-β1 induction
format: Article
creator:
  • Zhu, Weimin
  • Chen, Kang
  • Lu, Wei
  • Sun, Qifeng
  • Peng, Liangquan
  • Fen, Wenzhe
  • Li, Hao
  • Ou, Yangkan
  • Liu, Haifeng
  • Wang, Daping
  • Zeng, Yanjun
subjects:
  • Animal Genetics and Genomics
  • Animals
  • Article
  • Biocompatible Materials
  • Biomedical and Life Sciences
  • BIOTECHNOLOGY
  • Bone Marrow Cells - cytology
  • Bone Marrow Cells - drug effects
  • Bones
  • Cartilage
  • Cell adhesion
  • Cell Biology
  • Cell Culture
  • Cell Culture Techniques
  • Cell Differentiation
  • Chondrocytes
  • Collagens
  • Cultured cells
  • Developmental Biology
  • Life Sciences
  • Rabbits
  • Reverse transcriptase polymerase chain reaction
  • Scaffolds
  • Stem Cells
  • Stem Cells - cytology
  • Stem Cells - drug effects
  • Tissue engineering
  • Tissue Engineering - methods
  • Tissue Scaffolds
  • Toluidines
  • Transforming Growth Factor beta1 - pharmacology
ispartof: In vitro cellular & developmental biology. Animal, 2014-03-01, Vol.50 (3), p.214-220
description: The aim of this study is to investigate the effects of differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs) into chondrocytes induced by transforming growth factor-betal (TCP-β1) composite poly-lactide-co-glycolic acid/nano-hydroxyapatite (PLLA/nano-HA) to the construction of biomimetic artificial cartilage in vitro. In the lowtemperature extrusion preparation of PLLA/nano-HA composite porous scaffolds, rBMSCs were isolated and cultured to third generation in vitro, induced by TGF-β1-contained special inducing system into chondrocytes, 14 d later, identified by toluidine blue and type II collagen immunohistochemistry staining, and then the differential chondrocytes composite into the PLLA/nano-HA composite porous scaffolds, using scanning electron microscopy (SEM) to observe the growth conditions and cell attachment on the composite in the 7th, 14th, and 21st day and to gather cells on composite in the 7th, 14th, and 21st day of cell. RT-PCR is used to detect the expression of aggrecan (Col2A1 in mRNA) and Western blot for detection of the expression of type II collagen of the attached cells. rBMSCs can differentiate into chondrocytes when induced, and the differentiation of chondrocytes secreting GAG by toluidine blue staining and type II collagen immunohistochemistry staining was positive; SEM confirm the cells distribution evenly, stretching well in composite. RTPCR of aggrecan, Col2A1 in mRNA, and Western-blot of type II collagen expression in the differentiation of chondrocytes have different levels. Using TGF-β1 containing special inducing system induced rBMSCs into chondrocytes, then into compounds of PLLA/nano-HA composite porous scaffolds, and cell carrier complex proliferated well and secreted the chondrocyte-specific extracellular matrix stably, successfully constructing artificial bionic in vitro.
language: eng
source:
identifier: ISSN: 1071-2690
fulltext: no_fulltext
issn:
  • 1071-2690
  • 1543-706X
url: Link


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titleIn vitro study of nano-HA/PLLA composite scaffold for rabbit BMSC differentiation under TGF-β1 induction
creatorZhu, Weimin ; Chen, Kang ; Lu, Wei ; Sun, Qifeng ; Peng, Liangquan ; Fen, Wenzhe ; Li, Hao ; Ou, Yangkan ; Liu, Haifeng ; Wang, Daping ; Zeng, Yanjun
creatorcontribZhu, Weimin ; Chen, Kang ; Lu, Wei ; Sun, Qifeng ; Peng, Liangquan ; Fen, Wenzhe ; Li, Hao ; Ou, Yangkan ; Liu, Haifeng ; Wang, Daping ; Zeng, Yanjun
descriptionThe aim of this study is to investigate the effects of differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs) into chondrocytes induced by transforming growth factor-betal (TCP-β1) composite poly-lactide-co-glycolic acid/nano-hydroxyapatite (PLLA/nano-HA) to the construction of biomimetic artificial cartilage in vitro. In the lowtemperature extrusion preparation of PLLA/nano-HA composite porous scaffolds, rBMSCs were isolated and cultured to third generation in vitro, induced by TGF-β1-contained special inducing system into chondrocytes, 14 d later, identified by toluidine blue and type II collagen immunohistochemistry staining, and then the differential chondrocytes composite into the PLLA/nano-HA composite porous scaffolds, using scanning electron microscopy (SEM) to observe the growth conditions and cell attachment on the composite in the 7th, 14th, and 21st day and to gather cells on composite in the 7th, 14th, and 21st day of cell. RT-PCR is used to detect the expression of aggrecan (Col2A1 in mRNA) and Western blot for detection of the expression of type II collagen of the attached cells. rBMSCs can differentiate into chondrocytes when induced, and the differentiation of chondrocytes secreting GAG by toluidine blue staining and type II collagen immunohistochemistry staining was positive; SEM confirm the cells distribution evenly, stretching well in composite. RTPCR of aggrecan, Col2A1 in mRNA, and Western-blot of type II collagen expression in the differentiation of chondrocytes have different levels. Using TGF-β1 containing special inducing system induced rBMSCs into chondrocytes, then into compounds of PLLA/nano-HA composite porous scaffolds, and cell carrier complex proliferated well and secreted the chondrocyte-specific extracellular matrix stably, successfully constructing artificial bionic in vitro.
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subjectAnimal Genetics and Genomics ; Animals ; Article ; Biocompatible Materials ; Biomedical and Life Sciences ; BIOTECHNOLOGY ; Bone Marrow Cells - cytology ; Bone Marrow Cells - drug effects ; Bones ; Cartilage ; Cell adhesion ; Cell Biology ; Cell Culture ; Cell Culture Techniques ; Cell Differentiation ; Chondrocytes ; Collagens ; Cultured cells ; Developmental Biology ; Life Sciences ; Rabbits ; Reverse transcriptase polymerase chain reaction ; Scaffolds ; Stem Cells ; Stem Cells - cytology ; Stem Cells - drug effects ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds ; Toluidines ; Transforming Growth Factor beta1 - pharmacology
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descriptionThe aim of this study is to investigate the effects of differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs) into chondrocytes induced by transforming growth factor-betal (TCP-β1) composite poly-lactide-co-glycolic acid/nano-hydroxyapatite (PLLA/nano-HA) to the construction of biomimetic artificial cartilage in vitro. In the lowtemperature extrusion preparation of PLLA/nano-HA composite porous scaffolds, rBMSCs were isolated and cultured to third generation in vitro, induced by TGF-β1-contained special inducing system into chondrocytes, 14 d later, identified by toluidine blue and type II collagen immunohistochemistry staining, and then the differential chondrocytes composite into the PLLA/nano-HA composite porous scaffolds, using scanning electron microscopy (SEM) to observe the growth conditions and cell attachment on the composite in the 7th, 14th, and 21st day and to gather cells on composite in the 7th, 14th, and 21st day of cell. RT-PCR is used to detect the expression of aggrecan (Col2A1 in mRNA) and Western blot for detection of the expression of type II collagen of the attached cells. rBMSCs can differentiate into chondrocytes when induced, and the differentiation of chondrocytes secreting GAG by toluidine blue staining and type II collagen immunohistochemistry staining was positive; SEM confirm the cells distribution evenly, stretching well in composite. RTPCR of aggrecan, Col2A1 in mRNA, and Western-blot of type II collagen expression in the differentiation of chondrocytes have different levels. Using TGF-β1 containing special inducing system induced rBMSCs into chondrocytes, then into compounds of PLLA/nano-HA composite porous scaffolds, and cell carrier complex proliferated well and secreted the chondrocyte-specific extracellular matrix stably, successfully constructing artificial bionic in vitro.
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titleIn vitro study of nano-HA/PLLA composite scaffold for rabbit BMSC differentiation under TGF-β1 induction
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abstractThe aim of this study is to investigate the effects of differentiation of rabbit bone marrow mesenchymal stem cells (rBMSCs) into chondrocytes induced by transforming growth factor-betal (TCP-β1) composite poly-lactide-co-glycolic acid/nano-hydroxyapatite (PLLA/nano-HA) to the construction of biomimetic artificial cartilage in vitro. In the lowtemperature extrusion preparation of PLLA/nano-HA composite porous scaffolds, rBMSCs were isolated and cultured to third generation in vitro, induced by TGF-β1-contained special inducing system into chondrocytes, 14 d later, identified by toluidine blue and type II collagen immunohistochemistry staining, and then the differential chondrocytes composite into the PLLA/nano-HA composite porous scaffolds, using scanning electron microscopy (SEM) to observe the growth conditions and cell attachment on the composite in the 7th, 14th, and 21st day and to gather cells on composite in the 7th, 14th, and 21st day of cell. RT-PCR is used to detect the expression of aggrecan (Col2A1 in mRNA) and Western blot for detection of the expression of type II collagen of the attached cells. rBMSCs can differentiate into chondrocytes when induced, and the differentiation of chondrocytes secreting GAG by toluidine blue staining and type II collagen immunohistochemistry staining was positive; SEM confirm the cells distribution evenly, stretching well in composite. RTPCR of aggrecan, Col2A1 in mRNA, and Western-blot of type II collagen expression in the differentiation of chondrocytes have different levels. Using TGF-β1 containing special inducing system induced rBMSCs into chondrocytes, then into compounds of PLLA/nano-HA composite porous scaffolds, and cell carrier complex proliferated well and secreted the chondrocyte-specific extracellular matrix stably, successfully constructing artificial bionic in vitro.
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