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miR-9 promotes cell proliferation and inhibits apoptosis by targeting LASS2 in bladder cancer

MicroRNA-9 upregulation was reported in several tumors. However, its function and mechanism in human bladder cancer remains obscure. The present study aims to identify the expression pattern, biological roles and potential mechanism of miR-9 in human bladder cancers. We found that expression level o... Full description

Journal Title: Tumor biology 2015-07-07, Vol.36 (12), p.9631-9640
Main Author: Wang, Haifeng
Other Authors: Zhang, Wei , Zuo, Yigang , Ding, Mingxia , Ke, Changxing , Yan, Ruping , Zhan, Hui , Liu, Jingyu , Wang, Jiansong
Format: Electronic Article Electronic Article
Language: English
Subjects:
RNA
Quelle: Alma/SFX Local Collection
Publisher: Dordrecht: Springer Netherlands
ID: ISSN: 1010-4283
Link: https://www.ncbi.nlm.nih.gov/pubmed/26150338
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recordid: cdi_pubmed_primary_26150338
title: miR-9 promotes cell proliferation and inhibits apoptosis by targeting LASS2 in bladder cancer
format: Article
creator:
  • Wang, Haifeng
  • Zhang, Wei
  • Zuo, Yigang
  • Ding, Mingxia
  • Ke, Changxing
  • Yan, Ruping
  • Zhan, Hui
  • Liu, Jingyu
  • Wang, Jiansong
subjects:
  • Apoptosis
  • Biomedical and Life Sciences
  • Biomedicine
  • Bladder cancer
  • Cancer Research
  • Cell adhesion & migration
  • Cell Line, Tumor
  • Cell Proliferation - genetics
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Luciferase
  • Membrane Proteins - biosynthesis
  • Membrane Proteins - genetics
  • MicroRNA
  • MicroRNAs - biosynthesis
  • MicroRNAs - genetics
  • Neoplasm Proteins - biosynthesis
  • Research Article
  • Ribonucleic acid
  • RNA
  • Sphingosine N-Acyltransferase - biosynthesis
  • Sphingosine N-Acyltransferase - genetics
  • Tumor Suppressor Proteins - biosynthesis
  • Tumor Suppressor Proteins - genetics
  • Urinary Bladder Neoplasms - genetics
  • Urinary Bladder Neoplasms - pathology
ispartof: Tumor biology, 2015-07-07, Vol.36 (12), p.9631-9640
description: MicroRNA-9 upregulation was reported in several tumors. However, its function and mechanism in human bladder cancer remains obscure. The present study aims to identify the expression pattern, biological roles and potential mechanism of miR-9 in human bladder cancers. We found that expression level of miR-9 in bladder cancer tissues was higher than normal tissues. miR-9 mimic transfection was performed in T24 and 5637 cells with low miR-9 expression, and miR-9 inhibitor was employed in BIU-87 cell line with high endogenous expression. miR-9 increased cell proliferation, cell cycle progression, invasion and chemoresistance, with upregulation of cyclin D1, MMP9, Bcl-2, and survivin and downregulation of E-cadherin. Using luciferase reporter assay, we confirmed that LASS2 was a direct target of miR-9 in bladder cancer cells. Transfection of miR-9 mimic downregulated LASS2 expression. LASS2 transfection downregulated Bcl-2 and survivin expression, which were induced by miR-9 mimic in both cell lines. In conclusion, these results indicate that miR-9 upregulation might be associated with malignant phenotype of bladder cancer. miR-9 promotes chemoresistance of bladder cancer cells by target LASS2.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 1010-4283
fulltext: fulltext
issn:
  • 1010-4283
  • 1423-0380
url: Link


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titlemiR-9 promotes cell proliferation and inhibits apoptosis by targeting LASS2 in bladder cancer
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descriptionMicroRNA-9 upregulation was reported in several tumors. However, its function and mechanism in human bladder cancer remains obscure. The present study aims to identify the expression pattern, biological roles and potential mechanism of miR-9 in human bladder cancers. We found that expression level of miR-9 in bladder cancer tissues was higher than normal tissues. miR-9 mimic transfection was performed in T24 and 5637 cells with low miR-9 expression, and miR-9 inhibitor was employed in BIU-87 cell line with high endogenous expression. miR-9 increased cell proliferation, cell cycle progression, invasion and chemoresistance, with upregulation of cyclin D1, MMP9, Bcl-2, and survivin and downregulation of E-cadherin. Using luciferase reporter assay, we confirmed that LASS2 was a direct target of miR-9 in bladder cancer cells. Transfection of miR-9 mimic downregulated LASS2 expression. LASS2 transfection downregulated Bcl-2 and survivin expression, which were induced by miR-9 mimic in both cell lines. In conclusion, these results indicate that miR-9 upregulation might be associated with malignant phenotype of bladder cancer. miR-9 promotes chemoresistance of bladder cancer cells by target LASS2.
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subjectApoptosis ; Biomedical and Life Sciences ; Biomedicine ; Bladder cancer ; Cancer Research ; Cell adhesion & migration ; Cell Line, Tumor ; Cell Proliferation - genetics ; Gene Expression Regulation, Neoplastic ; Humans ; Luciferase ; Membrane Proteins - biosynthesis ; Membrane Proteins - genetics ; MicroRNA ; MicroRNAs - biosynthesis ; MicroRNAs - genetics ; Neoplasm Proteins - biosynthesis ; Research Article ; Ribonucleic acid ; RNA ; Sphingosine N-Acyltransferase - biosynthesis ; Sphingosine N-Acyltransferase - genetics ; Tumor Suppressor Proteins - biosynthesis ; Tumor Suppressor Proteins - genetics ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - pathology
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descriptionMicroRNA-9 upregulation was reported in several tumors. However, its function and mechanism in human bladder cancer remains obscure. The present study aims to identify the expression pattern, biological roles and potential mechanism of miR-9 in human bladder cancers. We found that expression level of miR-9 in bladder cancer tissues was higher than normal tissues. miR-9 mimic transfection was performed in T24 and 5637 cells with low miR-9 expression, and miR-9 inhibitor was employed in BIU-87 cell line with high endogenous expression. miR-9 increased cell proliferation, cell cycle progression, invasion and chemoresistance, with upregulation of cyclin D1, MMP9, Bcl-2, and survivin and downregulation of E-cadherin. Using luciferase reporter assay, we confirmed that LASS2 was a direct target of miR-9 in bladder cancer cells. Transfection of miR-9 mimic downregulated LASS2 expression. LASS2 transfection downregulated Bcl-2 and survivin expression, which were induced by miR-9 mimic in both cell lines. In conclusion, these results indicate that miR-9 upregulation might be associated with malignant phenotype of bladder cancer. miR-9 promotes chemoresistance of bladder cancer cells by target LASS2.
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abstractMicroRNA-9 upregulation was reported in several tumors. However, its function and mechanism in human bladder cancer remains obscure. The present study aims to identify the expression pattern, biological roles and potential mechanism of miR-9 in human bladder cancers. We found that expression level of miR-9 in bladder cancer tissues was higher than normal tissues. miR-9 mimic transfection was performed in T24 and 5637 cells with low miR-9 expression, and miR-9 inhibitor was employed in BIU-87 cell line with high endogenous expression. miR-9 increased cell proliferation, cell cycle progression, invasion and chemoresistance, with upregulation of cyclin D1, MMP9, Bcl-2, and survivin and downregulation of E-cadherin. Using luciferase reporter assay, we confirmed that LASS2 was a direct target of miR-9 in bladder cancer cells. Transfection of miR-9 mimic downregulated LASS2 expression. LASS2 transfection downregulated Bcl-2 and survivin expression, which were induced by miR-9 mimic in both cell lines. In conclusion, these results indicate that miR-9 upregulation might be associated with malignant phenotype of bladder cancer. miR-9 promotes chemoresistance of bladder cancer cells by target LASS2.
copDordrecht
pubSpringer Netherlands
pmid26150338
doi10.1007/s13277-015-3713-7
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