schliessen

Filtern

 

Bibliotheken

Functional interrogation and mining of natively paired human V H :V L antibody repertoires

We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (V :V ) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we iden... Full description

Journal Title: Nature biotechnology 2018-02, Vol.36 (2), p.152
Main Author: Wang, Bo
Other Authors: DeKosky, Brandon J , Timm, Morgan R , Lee, Jiwon , Normandin, Erica , Misasi, John , Kong, Rui , McDaniel, Jonathan R , Delidakis, George , Leigh, Kendra E , Niezold, Thomas , Choi, Chang W , Viox, Elise G , Fahad, Ahmed , Cagigi, Alberto , Ploquin, Aurélie , Leung, Kwanyee , Yang, Eun Sung , Kong, Wing-Pui , Voss, William N , Schmidt, Aaron G , Moody, M Anthony , Ambrozak, David R , Henry, Amy R , Laboune, Farida , Ledgerwood, Julie E , Graham, Barney S , Connors, Mark , Douek, Daniel C , Sullivan, Nancy J , Ellington, Andrew D , Mascola, John R , Georgiou, George
Format: Electronic Article Electronic Article
Language: English
Publisher: United States
ID: EISSN: 1546-1696
Link: https://www.ncbi.nlm.nih.gov/pubmed/29309060
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_pubmed_primary_29309060
title: Functional interrogation and mining of natively paired human V H :V L antibody repertoires
format: Article
creator:
  • Wang, Bo
  • DeKosky, Brandon J
  • Timm, Morgan R
  • Lee, Jiwon
  • Normandin, Erica
  • Misasi, John
  • Kong, Rui
  • McDaniel, Jonathan R
  • Delidakis, George
  • Leigh, Kendra E
  • Niezold, Thomas
  • Choi, Chang W
  • Viox, Elise G
  • Fahad, Ahmed
  • Cagigi, Alberto
  • Ploquin, Aurélie
  • Leung, Kwanyee
  • Yang, Eun Sung
  • Kong, Wing-Pui
  • Voss, William N
  • Schmidt, Aaron G
  • Moody, M Anthony
  • Ambrozak, David R
  • Henry, Amy R
  • Laboune, Farida
  • Ledgerwood, Julie E
  • Graham, Barney S
  • Connors, Mark
  • Douek, Daniel C
  • Sullivan, Nancy J
  • Ellington, Andrew D
  • Mascola, John R
  • Georgiou, George
ispartof: Nature biotechnology, 2018-02, Vol.36 (2), p.152
description: We present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (V :V ) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.
language: eng
source:
identifier: EISSN: 1546-1696
fulltext: no_fulltext
issn:
  • 1546-1696
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.7292266
LOCALfalse
PrimoNMBib
record
control
sourceidpubmed
recordidTN_cdi_pubmed_primary_29309060
sourceformatXML
sourcesystemPC
sourcerecordid29309060
originalsourceidFETCH-LOGICAL-1226t-4effb74fc8acd1fdf2fdaf231e37c8099888c3fba0156a608443135300ccef6e0
addsrcrecordideNo1j8FKxDAURYMgzjj6C_J-oPDSpJnUnQyOIxTc6CzcDGnyMkbatKSt0L-3oq4u53K4cC_YmhdSZVyVasWuh-ETEZVU6oqt8lJgiQrX7H0_RTuGLpoGQhwppe5sfhhMdNCGGOIZOg9xKb-omaE3IZGDj6k1EY5wgPsjVIs8hrpzMyTqKY3d4gw37NKbZqDbv9ywt_3j6-6QVS9Pz7uHKuN5rsZMkvf1VnqrjXXcO597Z3wuOImt1ViWWmsrfG2QF8oo1FIKLgqBaC15Rbhhd7-7_VS35E59Cq1J8-n_JH4DSClOAA
sourcetypeIndex Database
isCDItrue
recordtypearticle
display
typearticle
titleFunctional interrogation and mining of natively paired human V H :V L antibody repertoires
creatorWang, Bo ; DeKosky, Brandon J ; Timm, Morgan R ; Lee, Jiwon ; Normandin, Erica ; Misasi, John ; Kong, Rui ; McDaniel, Jonathan R ; Delidakis, George ; Leigh, Kendra E ; Niezold, Thomas ; Choi, Chang W ; Viox, Elise G ; Fahad, Ahmed ; Cagigi, Alberto ; Ploquin, Aurélie ; Leung, Kwanyee ; Yang, Eun Sung ; Kong, Wing-Pui ; Voss, William N ; Schmidt, Aaron G ; Moody, M Anthony ; Ambrozak, David R ; Henry, Amy R ; Laboune, Farida ; Ledgerwood, Julie E ; Graham, Barney S ; Connors, Mark ; Douek, Daniel C ; Sullivan, Nancy J ; Ellington, Andrew D ; Mascola, John R ; Georgiou, George
creatorcontribWang, Bo ; DeKosky, Brandon J ; Timm, Morgan R ; Lee, Jiwon ; Normandin, Erica ; Misasi, John ; Kong, Rui ; McDaniel, Jonathan R ; Delidakis, George ; Leigh, Kendra E ; Niezold, Thomas ; Choi, Chang W ; Viox, Elise G ; Fahad, Ahmed ; Cagigi, Alberto ; Ploquin, Aurélie ; Leung, Kwanyee ; Yang, Eun Sung ; Kong, Wing-Pui ; Voss, William N ; Schmidt, Aaron G ; Moody, M Anthony ; Ambrozak, David R ; Henry, Amy R ; Laboune, Farida ; Ledgerwood, Julie E ; Graham, Barney S ; Connors, Mark ; Douek, Daniel C ; Sullivan, Nancy J ; Ellington, Andrew D ; Mascola, John R ; Georgiou, George
descriptionWe present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (V :V ) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.
identifier
0EISSN: 1546-1696
1PMID: 29309060
languageeng
publisherUnited States
ispartofNature biotechnology, 2018-02, Vol.36 (2), p.152
lds50peer_reviewed
oafree_for_read
orcidid0000-0002-6467-3330 ; 0000-0002-4330-9476
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29309060$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Wang, Bo
1DeKosky, Brandon J
2Timm, Morgan R
3Lee, Jiwon
4Normandin, Erica
5Misasi, John
6Kong, Rui
7McDaniel, Jonathan R
8Delidakis, George
9Leigh, Kendra E
10Niezold, Thomas
11Choi, Chang W
12Viox, Elise G
13Fahad, Ahmed
14Cagigi, Alberto
15Ploquin, Aurélie
16Leung, Kwanyee
17Yang, Eun Sung
18Kong, Wing-Pui
19Voss, William N
20Schmidt, Aaron G
21Moody, M Anthony
22Ambrozak, David R
23Henry, Amy R
24Laboune, Farida
25Ledgerwood, Julie E
26Graham, Barney S
27Connors, Mark
28Douek, Daniel C
29Sullivan, Nancy J
30Ellington, Andrew D
31Mascola, John R
32Georgiou, George
title
0Functional interrogation and mining of natively paired human V H :V L antibody repertoires
1Nature biotechnology
addtitleNat Biotechnol
descriptionWe present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (V :V ) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.
issn1546-1696
fulltextfalse
rsrctypearticle
creationdate2018
recordtypearticle
recordideNo1j8FKxDAURYMgzjj6C_J-oPDSpJnUnQyOIxTc6CzcDGnyMkbatKSt0L-3oq4u53K4cC_YmhdSZVyVasWuh-ETEZVU6oqt8lJgiQrX7H0_RTuGLpoGQhwppe5sfhhMdNCGGOIZOg9xKb-omaE3IZGDj6k1EY5wgPsjVIs8hrpzMyTqKY3d4gw37NKbZqDbv9ywt_3j6-6QVS9Pz7uHKuN5rsZMkvf1VnqrjXXcO597Z3wuOImt1ViWWmsrfG2QF8oo1FIKLgqBaC15Rbhhd7-7_VS35E59Cq1J8-n_JH4DSClOAA
startdate201802
enddate201802
creator
0Wang, Bo
1DeKosky, Brandon J
2Timm, Morgan R
3Lee, Jiwon
4Normandin, Erica
5Misasi, John
6Kong, Rui
7McDaniel, Jonathan R
8Delidakis, George
9Leigh, Kendra E
10Niezold, Thomas
11Choi, Chang W
12Viox, Elise G
13Fahad, Ahmed
14Cagigi, Alberto
15Ploquin, Aurélie
16Leung, Kwanyee
17Yang, Eun Sung
18Kong, Wing-Pui
19Voss, William N
20Schmidt, Aaron G
21Moody, M Anthony
22Ambrozak, David R
23Henry, Amy R
24Laboune, Farida
25Ledgerwood, Julie E
26Graham, Barney S
27Connors, Mark
28Douek, Daniel C
29Sullivan, Nancy J
30Ellington, Andrew D
31Mascola, John R
32Georgiou, George
scopeNPM
orcidid
0https://orcid.org/0000-0002-6467-3330
1https://orcid.org/0000-0002-4330-9476
sort
creationdate201802
titleFunctional interrogation and mining of natively paired human V H :V L antibody repertoires
authorWang, Bo ; DeKosky, Brandon J ; Timm, Morgan R ; Lee, Jiwon ; Normandin, Erica ; Misasi, John ; Kong, Rui ; McDaniel, Jonathan R ; Delidakis, George ; Leigh, Kendra E ; Niezold, Thomas ; Choi, Chang W ; Viox, Elise G ; Fahad, Ahmed ; Cagigi, Alberto ; Ploquin, Aurélie ; Leung, Kwanyee ; Yang, Eun Sung ; Kong, Wing-Pui ; Voss, William N ; Schmidt, Aaron G ; Moody, M Anthony ; Ambrozak, David R ; Henry, Amy R ; Laboune, Farida ; Ledgerwood, Julie E ; Graham, Barney S ; Connors, Mark ; Douek, Daniel C ; Sullivan, Nancy J ; Ellington, Andrew D ; Mascola, John R ; Georgiou, George
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1226t-4effb74fc8acd1fdf2fdaf231e37c8099888c3fba0156a608443135300ccef6e0
rsrctypearticles
prefilterarticles
languageeng
creationdate2018
toplevelpeer_reviewed
creatorcontrib
0Wang, Bo
1DeKosky, Brandon J
2Timm, Morgan R
3Lee, Jiwon
4Normandin, Erica
5Misasi, John
6Kong, Rui
7McDaniel, Jonathan R
8Delidakis, George
9Leigh, Kendra E
10Niezold, Thomas
11Choi, Chang W
12Viox, Elise G
13Fahad, Ahmed
14Cagigi, Alberto
15Ploquin, Aurélie
16Leung, Kwanyee
17Yang, Eun Sung
18Kong, Wing-Pui
19Voss, William N
20Schmidt, Aaron G
21Moody, M Anthony
22Ambrozak, David R
23Henry, Amy R
24Laboune, Farida
25Ledgerwood, Julie E
26Graham, Barney S
27Connors, Mark
28Douek, Daniel C
29Sullivan, Nancy J
30Ellington, Andrew D
31Mascola, John R
32Georgiou, George
collectionPubMed
jtitleNature biotechnology
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Wang, Bo
1DeKosky, Brandon J
2Timm, Morgan R
3Lee, Jiwon
4Normandin, Erica
5Misasi, John
6Kong, Rui
7McDaniel, Jonathan R
8Delidakis, George
9Leigh, Kendra E
10Niezold, Thomas
11Choi, Chang W
12Viox, Elise G
13Fahad, Ahmed
14Cagigi, Alberto
15Ploquin, Aurélie
16Leung, Kwanyee
17Yang, Eun Sung
18Kong, Wing-Pui
19Voss, William N
20Schmidt, Aaron G
21Moody, M Anthony
22Ambrozak, David R
23Henry, Amy R
24Laboune, Farida
25Ledgerwood, Julie E
26Graham, Barney S
27Connors, Mark
28Douek, Daniel C
29Sullivan, Nancy J
30Ellington, Andrew D
31Mascola, John R
32Georgiou, George
formatjournal
genrearticle
ristypeJOUR
atitleFunctional interrogation and mining of natively paired human V H :V L antibody repertoires
jtitleNature biotechnology
addtitleNat Biotechnol
date2018-02
risdate2018
volume36
issue2
spage152
pages152-
eissn1546-1696
abstractWe present a technology to screen millions of B cells for natively paired human antibody repertoires. Libraries of natively paired, variable region heavy and light (V :V ) amplicons are expressed in a yeast display platform that is optimized for human Fab surface expression. Using our method we identify HIV-1 broadly neutralizing antibodies (bNAbs) from an HIV-1 slow progressor and high-affinity neutralizing antibodies against Ebola virus glycoprotein and influenza hemagglutinin.
copUnited States
pmid29309060
orcidid
0https://orcid.org/0000-0002-6467-3330
1https://orcid.org/0000-0002-4330-9476
oafree_for_read