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Rapid dendritic cell recruitment to the bronchial mucosa of patients with atopic asthma in response to local allergen challenge

BACKGROUND Airway dendritic cells (DC) play an important role in chronic allergic airway inflammation in experimental animals, but a similar role for DC in human allergic asthma has been difficult to define. This pilot study was undertaken to elucidate the role of DC in allergic asthma by examining... Full description

Journal Title: Thorax 2001, Vol.56 (11), p.823-826
Main Author: Jahnsen, F L
Other Authors: Moloney, E D , Hogan, T , Upham, J W , Burke, C M , Holt, P G
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: London: BMJ Publishing Group Ltd and British Thoracic Society
ID: ISSN: 0040-6376
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recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1745967
title: Rapid dendritic cell recruitment to the bronchial mucosa of patients with atopic asthma in response to local allergen challenge
format: Article
creator:
  • Jahnsen, F L
  • Moloney, E D
  • Hogan, T
  • Upham, J W
  • Burke, C M
  • Holt, P G
subjects:
  • Adolescent
  • Adult
  • allergen challenge
  • Allergic diseases
  • Allergic reaction
  • Antigen presenting cells
  • Antigens, CD1 - immunology
  • Asthma
  • Asthma - immunology
  • Biological and medical sciences
  • Bronchi - immunology
  • Bronchial Provocation Tests - methods
  • Causes of
  • Complications
  • dendritic cells
  • Dendritic Cells - immunology
  • Female
  • Fluorescent Antibody Technique
  • Forced Expiratory Volume
  • HLA-DR Antigens - immunology
  • Humans
  • Immunity, Cellular
  • Immunopathology
  • Male
  • Measurement
  • Medical sciences
  • Middle Aged
  • Phenotype
  • Pilot Projects
  • Rapid Communication
  • Respiratory and ent allergic diseases
  • Respiratory Mucosa - immunology
  • respiratory system
  • respiratory tract diseases
  • Statistics, Nonparametric
ispartof: Thorax, 2001, Vol.56 (11), p.823-826
description: BACKGROUND Airway dendritic cells (DC) play an important role in chronic allergic airway inflammation in experimental animals, but a similar role for DC in human allergic asthma has been difficult to define. This pilot study was undertaken to elucidate the role of DC in allergic asthma by examining their potential to migrate to the lower airways in response to bronchial challenge with specific allergen. METHODS Bronchial biopsy specimens were obtained from seven patients with allergic asthma before and 4–5 hours after allergen challenge. Multicolour immunofluorescence staining was performed on mucosal cryosections to identify changes in the number and phenotypes of DC. RESULTS A dramatic increase in the number of CD1c+HLA-DR+ DC were observed in the lamina propria after challenge compared with baseline (22.4v 7.8 cells/mm2). The rapid accumulation (within 4–5 hours) of these cells strongly suggests that they were directly recruited from peripheral blood. CONCLUSION We have shown for the first time that a specific DC subset rapidly emigrates into the human bronchial mucosa during allergic inflammation. While this study is based on relatively few patients, the consistency of the overall results strongly suggests that the rapid population dynamics of human airway DC closely parallel those in animal models of acute inflammation. These findings support suggestions that DC have an important role in human airway allergy.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0040-6376
fulltext: fulltext
issn:
  • 0040-6376
  • 1468-3296
url: Link


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titleRapid dendritic cell recruitment to the bronchial mucosa of patients with atopic asthma in response to local allergen challenge
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descriptionBACKGROUND Airway dendritic cells (DC) play an important role in chronic allergic airway inflammation in experimental animals, but a similar role for DC in human allergic asthma has been difficult to define. This pilot study was undertaken to elucidate the role of DC in allergic asthma by examining their potential to migrate to the lower airways in response to bronchial challenge with specific allergen. METHODS Bronchial biopsy specimens were obtained from seven patients with allergic asthma before and 4–5 hours after allergen challenge. Multicolour immunofluorescence staining was performed on mucosal cryosections to identify changes in the number and phenotypes of DC. RESULTS A dramatic increase in the number of CD1c+HLA-DR+ DC were observed in the lamina propria after challenge compared with baseline (22.4v 7.8 cells/mm2). The rapid accumulation (within 4–5 hours) of these cells strongly suggests that they were directly recruited from peripheral blood. CONCLUSION We have shown for the first time that a specific DC subset rapidly emigrates into the human bronchial mucosa during allergic inflammation. While this study is based on relatively few patients, the consistency of the overall results strongly suggests that the rapid population dynamics of human airway DC closely parallel those in animal models of acute inflammation. These findings support suggestions that DC have an important role in human airway allergy.
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subjectAdolescent ; Adult ; allergen challenge ; Allergic diseases ; Allergic reaction ; Antigen presenting cells ; Antigens, CD1 - immunology ; Asthma ; Asthma - immunology ; Biological and medical sciences ; Bronchi - immunology ; Bronchial Provocation Tests - methods ; Causes of ; Complications ; dendritic cells ; Dendritic Cells - immunology ; Female ; Fluorescent Antibody Technique ; Forced Expiratory Volume ; HLA-DR Antigens - immunology ; Humans ; Immunity, Cellular ; Immunopathology ; Male ; Measurement ; Medical sciences ; Middle Aged ; Phenotype ; Pilot Projects ; Rapid Communication ; Respiratory and ent allergic diseases ; Respiratory Mucosa - immunology ; respiratory system ; respiratory tract diseases ; Statistics, Nonparametric
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descriptionBACKGROUND Airway dendritic cells (DC) play an important role in chronic allergic airway inflammation in experimental animals, but a similar role for DC in human allergic asthma has been difficult to define. This pilot study was undertaken to elucidate the role of DC in allergic asthma by examining their potential to migrate to the lower airways in response to bronchial challenge with specific allergen. METHODS Bronchial biopsy specimens were obtained from seven patients with allergic asthma before and 4–5 hours after allergen challenge. Multicolour immunofluorescence staining was performed on mucosal cryosections to identify changes in the number and phenotypes of DC. RESULTS A dramatic increase in the number of CD1c+HLA-DR+ DC were observed in the lamina propria after challenge compared with baseline (22.4v 7.8 cells/mm2). The rapid accumulation (within 4–5 hours) of these cells strongly suggests that they were directly recruited from peripheral blood. CONCLUSION We have shown for the first time that a specific DC subset rapidly emigrates into the human bronchial mucosa during allergic inflammation. While this study is based on relatively few patients, the consistency of the overall results strongly suggests that the rapid population dynamics of human airway DC closely parallel those in animal models of acute inflammation. These findings support suggestions that DC have an important role in human airway allergy.
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abstractBACKGROUND Airway dendritic cells (DC) play an important role in chronic allergic airway inflammation in experimental animals, but a similar role for DC in human allergic asthma has been difficult to define. This pilot study was undertaken to elucidate the role of DC in allergic asthma by examining their potential to migrate to the lower airways in response to bronchial challenge with specific allergen. METHODS Bronchial biopsy specimens were obtained from seven patients with allergic asthma before and 4–5 hours after allergen challenge. Multicolour immunofluorescence staining was performed on mucosal cryosections to identify changes in the number and phenotypes of DC. RESULTS A dramatic increase in the number of CD1c+HLA-DR+ DC were observed in the lamina propria after challenge compared with baseline (22.4v 7.8 cells/mm2). The rapid accumulation (within 4–5 hours) of these cells strongly suggests that they were directly recruited from peripheral blood. CONCLUSION We have shown for the first time that a specific DC subset rapidly emigrates into the human bronchial mucosa during allergic inflammation. While this study is based on relatively few patients, the consistency of the overall results strongly suggests that the rapid population dynamics of human airway DC closely parallel those in animal models of acute inflammation. These findings support suggestions that DC have an important role in human airway allergy.
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