schliessen

Filtern

 

Bibliotheken

Cover Image

Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases

We describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable. ZFNs induce a targeted double-strand break in the genome that is repaired to generate small insertions and deletions.... Full description

Journal Title: Nature biotechnology 2008-06, Vol.26 (6), p.702-708
Main Author: Doyon, Yannick
Other Authors: Rebar, Edward J , McCammon, Jasmine M , Amora, Rainier , Faraji, Farhoud , Miller, Jeffrey C , Gregory, Philip D , Ngo, Catherine , Amacher, Sharon L , Katibah, George E , Zhang, Lei , Hocking, Toby D , Urnov, Fyodor D
Format: Electronic Article Electronic Article
Language: English
Subjects:
Animal models in research
Animals
Animals, Genetically Modified - physiology
Article
Biological and medical sciences
Biotechnology
Danio rerio
Deoxyribonucleases - genetics
Fish
Fundamental and applied biological sciences. Psychology
fungi
Gene expression
Gene mutations
Gene Targeting - methods
Genetic aspects
Genetic Engineering - methods
Genotype & phenotype
Models
Mutagenesis, Site-Directed - methods
Mutation
Physiological aspects
Protein Engineering - methods
Ribonucleic acid
RNA
Usage
Zebra fish
Zebrafish - genetics
Zebrafish Proteins - genetics
Zinc finger proteins
Zinc Fingers - genetics
Publisher: New York, NY: Nature Publishing Group
ID: ISSN: 1087-0156
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2674762
title: Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
format: Article
creator:
  • Doyon, Yannick
  • Rebar, Edward J
  • McCammon, Jasmine M
  • Amora, Rainier
  • Faraji, Farhoud
  • Miller, Jeffrey C
  • Gregory, Philip D
  • Ngo, Catherine
  • Amacher, Sharon L
  • Katibah, George E
  • Zhang, Lei
  • Hocking, Toby D
  • Urnov, Fyodor D
subjects:
  • Animal models in research
  • Animals
  • Animals, Genetically Modified - physiology
  • Article
  • Biological and medical sciences
  • Biotechnology
  • Danio rerio
  • Deoxyribonucleases - genetics
  • Fish
  • Fundamental and applied biological sciences. Psychology
  • fungi
  • Gene expression
  • Gene mutations
  • Gene Targeting - methods
  • Genetic aspects
  • Genetic Engineering - methods
  • Genotype & phenotype
  • Models
  • Mutagenesis, Site-Directed - methods
  • Mutation
  • Physiological aspects
  • Protein Engineering - methods
  • Ribonucleic acid
  • RNA
  • Usage
  • Zebra fish
  • Zebrafish - genetics
  • Zebrafish Proteins - genetics
  • Zinc finger proteins
  • Zinc Fingers - genetics
ispartof: Nature biotechnology, 2008-06, Vol.26 (6), p.702-708
description: We describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable. ZFNs induce a targeted double-strand break in the genome that is repaired to generate small insertions and deletions. We designed ZFNs targeting the zebrafish golden and no tail/Brachyury (ntl) genes and developed a budding yeast-based assay to identify the most active ZFNs for use in vivo. Injection of ZFN-encoding mRNA into one-cell embryos yielded a high percentage of animals carrying distinct mutations at the ZFN-specified position and exhibiting expected loss-of-function phenotypes. Over half the ZFN mRNA-injected founder animals transmitted disrupted ntl alleles at frequencies averaging 20%. The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg.
language: eng
source:
identifier: ISSN: 1087-0156
fulltext: no_fulltext
issn:
  • 1087-0156
  • 1546-1696
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.7283225
LOCALfalse
PrimoNMBib
record
control
sourceidgale_pubme
recordidTN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2674762
sourceformatXML
sourcesystemPC
galeidA192352616
sourcerecordidA192352616
originalsourceidFETCH-LOGICAL-1729t-5d09f26d50c1a9e0779b7cd5af9053fa364320279dbbb2239ab4f62454945de50
addsrcrecordideNqNkm9r1TAUxosobk7xEyhFURHsTNL8ad4IY6h3MBjo9G1Ik9PejN70mqSi-_Sm3Ou8d-yFKSTl5Heec3r6FMVTjI4xqpv3vk2YInmvOMSM8gpzye_nd9SICmHGD4pHMV4hhDjl_GFxgBuGUF3Tw-JyAcEl3Q5QJh16SGDLHjyU1sUwrZMbfel8eQ1t0J2Ly3KKzvelheh6n9lr503V5RCE0k9mAB0hPi4edHqI8GR7HhXfPn28PF1U5xefz05PzissiEwVs0h2hFuGDNYSkBCyFcYy3UnE6k7XnNYEESFt27aE1FK3tOOEMiops8DQUfFho7ue2hVYAz4FPah1cCsdfqtRO7V_491S9eNPRbiggpMssNgIjGvw2gXYy7UekhrtTCvUGBCtaHBbC4qoqC3GXc1q3MxdAs9Sr7e9hPHHBDGplYsGhkF7GKeosBQyf8Nc88Ut8Gqcgs9zUiSvRlDSZOjlBur1AMr5bsz9m1lRnWBJakY4nmse30Hlx8LKmdFD53J8L-HtXkJmEvxKvZ5iVGdfv_w_e_F9n323w7azRyDmLZtkmeImZQ9_s8FNGGMM0N2MHSM1-1lt_ZzJ57t_-B-3NXAGXm0BHY0euqC9cfGGI0hIzpnIXHWrpMm2n-2dR-aGOwo_2_Bep2nHF3_v_wAl2xHU
sourcetypeOpen Access Repository
isCDItrue
recordtypearticle
pqid222287428
display
typearticle
titleHeritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
creatorDoyon, Yannick ; Rebar, Edward J ; McCammon, Jasmine M ; Amora, Rainier ; Faraji, Farhoud ; Miller, Jeffrey C ; Gregory, Philip D ; Ngo, Catherine ; Amacher, Sharon L ; Katibah, George E ; Zhang, Lei ; Hocking, Toby D ; Urnov, Fyodor D
creatorcontribDoyon, Yannick ; Rebar, Edward J ; McCammon, Jasmine M ; Amora, Rainier ; Faraji, Farhoud ; Miller, Jeffrey C ; Gregory, Philip D ; Ngo, Catherine ; Amacher, Sharon L ; Katibah, George E ; Zhang, Lei ; Hocking, Toby D ; Urnov, Fyodor D
descriptionWe describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable. ZFNs induce a targeted double-strand break in the genome that is repaired to generate small insertions and deletions. We designed ZFNs targeting the zebrafish golden and no tail/Brachyury (ntl) genes and developed a budding yeast-based assay to identify the most active ZFNs for use in vivo. Injection of ZFN-encoding mRNA into one-cell embryos yielded a high percentage of animals carrying distinct mutations at the ZFN-specified position and exhibiting expected loss-of-function phenotypes. Over half the ZFN mRNA-injected founder animals transmitted disrupted ntl alleles at frequencies averaging 20%. The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg.
identifier
0ISSN: 1087-0156
1EISSN: 1546-1696
2DOI: 10.1038/nbt1409
3PMID: 18500334
4CODEN: NABIF9
languageeng
publisherNew York, NY: Nature Publishing Group
subjectAnimal models in research ; Animals ; Animals, Genetically Modified - physiology ; Article ; Biological and medical sciences ; Biotechnology ; Danio rerio ; Deoxyribonucleases - genetics ; Fish ; Fundamental and applied biological sciences. Psychology ; fungi ; Gene expression ; Gene mutations ; Gene Targeting - methods ; Genetic aspects ; Genetic Engineering - methods ; Genotype & phenotype ; Models ; Mutagenesis, Site-Directed - methods ; Mutation ; Physiological aspects ; Protein Engineering - methods ; Ribonucleic acid ; RNA ; Usage ; Zebra fish ; Zebrafish - genetics ; Zebrafish Proteins - genetics ; Zinc finger proteins ; Zinc Fingers - genetics
ispartofNature biotechnology, 2008-06, Vol.26 (6), p.702-708
rights
02009 INIST-CNRS
1COPYRIGHT 2008 Nature Publishing Group
2Copyright Nature Publishing Group Jun 2008
lds50peer_reviewed
oafree_for_read
citedbyFETCH-LOGICAL-1729t-5d09f26d50c1a9e0779b7cd5af9053fa364320279dbbb2239ab4f62454945de50
citesFETCH-LOGICAL-1729t-5d09f26d50c1a9e0779b7cd5af9053fa364320279dbbb2239ab4f62454945de50
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink
0$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20796657$$DView record in Pascal Francis
1$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18500334$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Doyon, Yannick
1Rebar, Edward J
2McCammon, Jasmine M
3Amora, Rainier
4Faraji, Farhoud
5Miller, Jeffrey C
6Gregory, Philip D
7Ngo, Catherine
8Amacher, Sharon L
9Katibah, George E
10Zhang, Lei
11Hocking, Toby D
12Urnov, Fyodor D
title
0Heritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
1Nature biotechnology
addtitleNat Biotechnol
descriptionWe describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable. ZFNs induce a targeted double-strand break in the genome that is repaired to generate small insertions and deletions. We designed ZFNs targeting the zebrafish golden and no tail/Brachyury (ntl) genes and developed a budding yeast-based assay to identify the most active ZFNs for use in vivo. Injection of ZFN-encoding mRNA into one-cell embryos yielded a high percentage of animals carrying distinct mutations at the ZFN-specified position and exhibiting expected loss-of-function phenotypes. Over half the ZFN mRNA-injected founder animals transmitted disrupted ntl alleles at frequencies averaging 20%. The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg.
subject
0Animal models in research
1Animals
2Animals, Genetically Modified - physiology
3Article
4Biological and medical sciences
5Biotechnology
6Danio rerio
7Deoxyribonucleases - genetics
8Fish
9Fundamental and applied biological sciences. Psychology
10fungi
11Gene expression
12Gene mutations
13Gene Targeting - methods
14Genetic aspects
15Genetic Engineering - methods
16Genotype & phenotype
17Models
18Mutagenesis, Site-Directed - methods
19Mutation
20Physiological aspects
21Protein Engineering - methods
22Ribonucleic acid
23RNA
24Usage
25Zebra fish
26Zebrafish - genetics
27Zebrafish Proteins - genetics
28Zinc finger proteins
29Zinc Fingers - genetics
issn
01087-0156
11546-1696
fulltextfalse
rsrctypearticle
creationdate2008
recordtypearticle
recordideNqNkm9r1TAUxosobk7xEyhFURHsTNL8ad4IY6h3MBjo9G1Ik9PejN70mqSi-_Sm3Ou8d-yFKSTl5Heec3r6FMVTjI4xqpv3vk2YInmvOMSM8gpzye_nd9SICmHGD4pHMV4hhDjl_GFxgBuGUF3Tw-JyAcEl3Q5QJh16SGDLHjyU1sUwrZMbfel8eQ1t0J2Ly3KKzvelheh6n9lr503V5RCE0k9mAB0hPi4edHqI8GR7HhXfPn28PF1U5xefz05PzissiEwVs0h2hFuGDNYSkBCyFcYy3UnE6k7XnNYEESFt27aE1FK3tOOEMiops8DQUfFho7ue2hVYAz4FPah1cCsdfqtRO7V_491S9eNPRbiggpMssNgIjGvw2gXYy7UekhrtTCvUGBCtaHBbC4qoqC3GXc1q3MxdAs9Sr7e9hPHHBDGplYsGhkF7GKeosBQyf8Nc88Ut8Gqcgs9zUiSvRlDSZOjlBur1AMr5bsz9m1lRnWBJakY4nmse30Hlx8LKmdFD53J8L-HtXkJmEvxKvZ5iVGdfv_w_e_F9n323w7azRyDmLZtkmeImZQ9_s8FNGGMM0N2MHSM1-1lt_ZzJ57t_-B-3NXAGXm0BHY0euqC9cfGGI0hIzpnIXHWrpMm2n-2dR-aGOwo_2_Bep2nHF3_v_wAl2xHU
startdate200806
enddate200806
creator
0Doyon, Yannick
1Rebar, Edward J
2McCammon, Jasmine M
3Amora, Rainier
4Faraji, Farhoud
5Miller, Jeffrey C
6Gregory, Philip D
7Ngo, Catherine
8Amacher, Sharon L
9Katibah, George E
10Zhang, Lei
11Hocking, Toby D
12Urnov, Fyodor D
generalNature Publishing Group
scope
0IQODW
1CGR
2CUY
3CVF
4ECM
5EIF
6NPM
7AAYXX
8CITATION
93V.
107QO
117QP
127QR
137T7
147TK
157TM
167X7
177XB
1888A
1988E
2088I
218AO
228FD
238FE
248FG
258FH
268FI
278FJ
288FK
298G5
30ABJCF
31ABUWG
32AZQEC
33BBNVY
34BENPR
35BGLVJ
36BHPHI
37C1K
38DWQXO
39FR3
40FYUFA
41GHDGH
42GNUQQ
43GUQSH
44HCIFZ
45K9.
46L6V
47LK8
48M0S
49M1P
50M2O
51M2P
52M7P
53M7S
54MBDVC
55P64
56PADUT
57PQEST
58PQQKQ
59PQUKI
60PRINS
61PTHSS
62Q9U
63RC3
64BOBZL
65CLFQK
665PM
sort
creationdate200806
titleHeritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
authorDoyon, Yannick ; Rebar, Edward J ; McCammon, Jasmine M ; Amora, Rainier ; Faraji, Farhoud ; Miller, Jeffrey C ; Gregory, Philip D ; Ngo, Catherine ; Amacher, Sharon L ; Katibah, George E ; Zhang, Lei ; Hocking, Toby D ; Urnov, Fyodor D
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1729t-5d09f26d50c1a9e0779b7cd5af9053fa364320279dbbb2239ab4f62454945de50
rsrctypearticles
prefilterarticles
languageeng
creationdate2008
topic
0Animal models in research
1Animals
2Animals, Genetically Modified - physiology
3Article
4Biological and medical sciences
5Biotechnology
6Danio rerio
7Deoxyribonucleases - genetics
8Fish
9Fundamental and applied biological sciences. Psychology
10fungi
11Gene expression
12Gene mutations
13Gene Targeting - methods
14Genetic aspects
15Genetic Engineering - methods
16Genotype & phenotype
17Models
18Mutagenesis, Site-Directed - methods
19Mutation
20Physiological aspects
21Protein Engineering - methods
22Ribonucleic acid
23RNA
24Usage
25Zebra fish
26Zebrafish - genetics
27Zebrafish Proteins - genetics
28Zinc finger proteins
29Zinc Fingers - genetics
toplevelpeer_reviewed
creatorcontrib
0Doyon, Yannick
1Rebar, Edward J
2McCammon, Jasmine M
3Amora, Rainier
4Faraji, Farhoud
5Miller, Jeffrey C
6Gregory, Philip D
7Ngo, Catherine
8Amacher, Sharon L
9Katibah, George E
10Zhang, Lei
11Hocking, Toby D
12Urnov, Fyodor D
collection
0Pascal-Francis
1Medline
2MEDLINE
3MEDLINE (Ovid)
4MEDLINE
5MEDLINE
6PubMed
7CrossRef
8ProQuest Central (Corporate)
9Biotechnology Research Abstracts
10Calcium & Calcified Tissue Abstracts
11Chemoreception Abstracts
12Industrial and Applied Microbiology Abstracts (Microbiology A)
13Neurosciences Abstracts
14Nucleic Acids Abstracts
15Health & Medical Collection
16ProQuest Central (purchase pre-March 2016)
17Biology Database (Alumni Edition)
18Medical Database (Alumni Edition)
19Science Database (Alumni Edition)
20ProQuest Pharma Collection
21Technology Research Database
22ProQuest SciTech Collection
23ProQuest Technology Collection
24ProQuest Natural Science Collection
25Hospital Premium Collection
26Hospital Premium Collection (Alumni Edition)
27ProQuest Central (Alumni) (purchase pre-March 2016)
28Research Library (Alumni Edition)
29Materials Science & Engineering Collection
30ProQuest Central (Alumni Edition)
31ProQuest Central Essentials
32Biological Science Collection
33ProQuest Central
34Technology Collection
35Natural Science Collection
36Environmental Sciences and Pollution Management
37ProQuest Central Korea
38Engineering Research Database
39Health Research Premium Collection
40Health Research Premium Collection (Alumni)
41ProQuest Central Student
42Research Library Prep
43SciTech Premium Collection
44ProQuest Health & Medical Complete (Alumni)
45ProQuest Engineering Collection
46ProQuest Biological Science Collection
47Health & Medical Collection (Alumni Edition)
48Medical Database
49Research Library
50Science Database
51Biological Science Database
52Engineering Database
53Research Library (Corporate)
54Biotechnology and BioEngineering Abstracts
55Research Library China
56ProQuest One Academic Eastern Edition
57ProQuest One Academic
58ProQuest One Academic UKI Edition
59ProQuest Central China
60Engineering Collection
61ProQuest Central Basic
62Genetics Abstracts
63OpenAIRE (Open Access)
64OpenAIRE
65PubMed Central (Full Participant titles)
jtitleNature biotechnology
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Doyon, Yannick
1Rebar, Edward J
2McCammon, Jasmine M
3Amora, Rainier
4Faraji, Farhoud
5Miller, Jeffrey C
6Gregory, Philip D
7Ngo, Catherine
8Amacher, Sharon L
9Katibah, George E
10Zhang, Lei
11Hocking, Toby D
12Urnov, Fyodor D
formatjournal
genrearticle
ristypeJOUR
atitleHeritable targeted gene disruption in zebrafish using designed zinc-finger nucleases
jtitleNature biotechnology
addtitleNat Biotechnol
date2008-06
risdate2008
volume26
issue6
spage702
epage708
pages702-708
issn1087-0156
eissn1546-1696
codenNABIF9
notesThese authors contributed equally to this work.
abstractWe describe the use of zinc-finger nucleases (ZFNs) for somatic and germline disruption of genes in zebrafish (Danio rerio), in which targeted mutagenesis was previously intractable. ZFNs induce a targeted double-strand break in the genome that is repaired to generate small insertions and deletions. We designed ZFNs targeting the zebrafish golden and no tail/Brachyury (ntl) genes and developed a budding yeast-based assay to identify the most active ZFNs for use in vivo. Injection of ZFN-encoding mRNA into one-cell embryos yielded a high percentage of animals carrying distinct mutations at the ZFN-specified position and exhibiting expected loss-of-function phenotypes. Over half the ZFN mRNA-injected founder animals transmitted disrupted ntl alleles at frequencies averaging 20%. The frequency and precision of gene-disruption events observed suggest that this approach should be applicable to any loci in zebrafish or in other organisms that allow mRNA delivery into the fertilized egg.
copNew York, NY
pubNature Publishing Group
pmid18500334
doi10.1038/nbt1409
tpages7
oafree_for_read