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Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa

Chemical imaging by confocal Raman microscopy has been used for the visualization of the cellulose and lignin distribution in wood cell walls. Lignin reduction in wood can be achieved by, for example, transgenic suppression of a monolignol biosynthesis gene encoding 4-coumarate-CoA ligase (4CL). Her... Full description

Journal Title: Planta 2009, Vol.230 (3), p.589-597
Main Author: Schmidt, M
Other Authors: Schwartzberg, A. M , Perera, P. N , Weber-Bargioni, A , Carroll, A , Sarkar, P , Bosneaga, E , Urban, J. J , Song, J , Balakshin, M. Y , Capanema, E. A , Auer, M , Adams, P. D , Chiang, V. L , Schuck, P. James
Format: Electronic Article Electronic Article
Language: English
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Publisher: Berlin/Heidelberg: Springer-Verlag
ID: ISSN: 0032-0935
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title: Label-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa
format: Article
creator:
  • Schmidt, M
  • Schwartzberg, A. M
  • Perera, P. N
  • Weber-Bargioni, A
  • Carroll, A
  • Sarkar, P
  • Bosneaga, E
  • Urban, J. J
  • Song, J
  • Balakshin, M. Y
  • Capanema, E. A
  • Auer, M
  • Adams, P. D
  • Chiang, V. L
  • Schuck, P. James
subjects:
  • Agriculture
  • beverages
  • Biofuels
  • Biological and medical sciences
  • Biomedical and Life Sciences
  • Cell wall
  • Cell Wall - metabolism
  • Cell walls
  • Cellulose
  • complex mixtures
  • Ecology
  • Fibers
  • food
  • Forestry
  • Foundries
  • Fundamental and applied biological sciences. Psychology
  • fungi
  • Genetic engineering
  • Genetics
  • Imaging
  • industry
  • Life Sciences
  • Ligases
  • Lignin
  • Lignin - metabolism
  • macromolecular substances
  • Medicine, Botanic
  • Medicine, Herbal
  • Microscopy
  • Microspectroscopy
  • Plant cells
  • Plant Science
  • Plant Sciences
  • Plants
  • Plants, Genetically Modified - cytology
  • Plants, Genetically Modified - metabolism
  • Populus - cytology
  • Populus - metabolism
  • Populus trichocarpa
  • Populus trichocarpa (black cottonwood)
  • Raman imaging
  • Raman scattering
  • Raman spectroscopy
  • RAPID COMMUNICATION
  • Spectrum Analysis, Raman
  • technology
  • Tension wood
  • Transgenic
  • Transgenic plants
ispartof: Planta, 2009, Vol.230 (3), p.589-597
description: Chemical imaging by confocal Raman microscopy has been used for the visualization of the cellulose and lignin distribution in wood cell walls. Lignin reduction in wood can be achieved by, for example, transgenic suppression of a monolignol biosynthesis gene encoding 4-coumarate-CoA ligase (4CL). Here, we use confocal Raman microscopy to compare lignification in wild type and lignin-reduced 4CL transgenic Populus trichocarpa stem wood with spatial resolution that is sub-μm. Analyzing the lignin Raman bands in the spectral region between 1,600 and 1,700 cm-1, differences in lignin signal intensity and localization are mapped in situ. Transgenic reduction of lignin is particularly pronounced in the S2 wall layer of fibers, suggesting that such transgenic approach may help overcome cell wall recalcitrance to wood saccharification. Spatial heterogeneity in the lignin composition, in particular with regard to ethylenic residues, is observed in both samples.
language: eng
source:
identifier: ISSN: 0032-0935
fulltext: no_fulltext
issn:
  • 0032-0935
  • 1432-2048
url: Link


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titleLabel-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa
creatorSchmidt, M ; Schwartzberg, A. M ; Perera, P. N ; Weber-Bargioni, A ; Carroll, A ; Sarkar, P ; Bosneaga, E ; Urban, J. J ; Song, J ; Balakshin, M. Y ; Capanema, E. A ; Auer, M ; Adams, P. D ; Chiang, V. L ; Schuck, P. James
creatorcontribSchmidt, M ; Schwartzberg, A. M ; Perera, P. N ; Weber-Bargioni, A ; Carroll, A ; Sarkar, P ; Bosneaga, E ; Urban, J. J ; Song, J ; Balakshin, M. Y ; Capanema, E. A ; Auer, M ; Adams, P. D ; Chiang, V. L ; Schuck, P. James
descriptionChemical imaging by confocal Raman microscopy has been used for the visualization of the cellulose and lignin distribution in wood cell walls. Lignin reduction in wood can be achieved by, for example, transgenic suppression of a monolignol biosynthesis gene encoding 4-coumarate-CoA ligase (4CL). Here, we use confocal Raman microscopy to compare lignification in wild type and lignin-reduced 4CL transgenic Populus trichocarpa stem wood with spatial resolution that is sub-μm. Analyzing the lignin Raman bands in the spectral region between 1,600 and 1,700 cm-1, differences in lignin signal intensity and localization are mapped in situ. Transgenic reduction of lignin is particularly pronounced in the S2 wall layer of fibers, suggesting that such transgenic approach may help overcome cell wall recalcitrance to wood saccharification. Spatial heterogeneity in the lignin composition, in particular with regard to ethylenic residues, is observed in both samples.
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publisherBerlin/Heidelberg: Springer-Verlag
subjectAgriculture ; beverages ; Biofuels ; Biological and medical sciences ; Biomedical and Life Sciences ; Cell wall ; Cell Wall - metabolism ; Cell walls ; Cellulose ; complex mixtures ; Ecology ; Fibers ; food ; Forestry ; Foundries ; Fundamental and applied biological sciences. Psychology ; fungi ; Genetic engineering ; Genetics ; Imaging ; industry ; Life Sciences ; Ligases ; Lignin ; Lignin - metabolism ; macromolecular substances ; Medicine, Botanic ; Medicine, Herbal ; Microscopy ; Microspectroscopy ; Plant cells ; Plant Science ; Plant Sciences ; Plants ; Plants, Genetically Modified - cytology ; Plants, Genetically Modified - metabolism ; Populus - cytology ; Populus - metabolism ; Populus trichocarpa ; Populus trichocarpa (black cottonwood) ; Raman imaging ; Raman scattering ; Raman spectroscopy ; RAPID COMMUNICATION ; Spectrum Analysis, Raman ; technology ; Tension wood ; Transgenic ; Transgenic plants
ispartofPlanta, 2009, Vol.230 (3), p.589-597
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2Perera, P. N
3Weber-Bargioni, A
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6Bosneaga, E
7Urban, J. J
8Song, J
9Balakshin, M. Y
10Capanema, E. A
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13Chiang, V. L
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descriptionChemical imaging by confocal Raman microscopy has been used for the visualization of the cellulose and lignin distribution in wood cell walls. Lignin reduction in wood can be achieved by, for example, transgenic suppression of a monolignol biosynthesis gene encoding 4-coumarate-CoA ligase (4CL). Here, we use confocal Raman microscopy to compare lignification in wild type and lignin-reduced 4CL transgenic Populus trichocarpa stem wood with spatial resolution that is sub-μm. Analyzing the lignin Raman bands in the spectral region between 1,600 and 1,700 cm-1, differences in lignin signal intensity and localization are mapped in situ. Transgenic reduction of lignin is particularly pronounced in the S2 wall layer of fibers, suggesting that such transgenic approach may help overcome cell wall recalcitrance to wood saccharification. Spatial heterogeneity in the lignin composition, in particular with regard to ethylenic residues, is observed in both samples.
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0Agriculture
1beverages
2Biofuels
3Biological and medical sciences
4Biomedical and Life Sciences
5Cell wall
6Cell Wall - metabolism
7Cell walls
8Cellulose
9complex mixtures
10Ecology
11Fibers
12food
13Forestry
14Foundries
15Fundamental and applied biological sciences. Psychology
16fungi
17Genetic engineering
18Genetics
19Imaging
20industry
21Life Sciences
22Ligases
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24Lignin - metabolism
25macromolecular substances
26Medicine, Botanic
27Medicine, Herbal
28Microscopy
29Microspectroscopy
30Plant cells
31Plant Science
32Plant Sciences
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34Plants, Genetically Modified - cytology
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36Populus - cytology
37Populus - metabolism
38Populus trichocarpa
39Populus trichocarpa (black cottonwood)
40Raman imaging
41Raman scattering
42Raman spectroscopy
43RAPID COMMUNICATION
44Spectrum Analysis, Raman
45technology
46Tension wood
47Transgenic
48Transgenic plants
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titleLabel-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa
authorSchmidt, M ; Schwartzberg, A. M ; Perera, P. N ; Weber-Bargioni, A ; Carroll, A ; Sarkar, P ; Bosneaga, E ; Urban, J. J ; Song, J ; Balakshin, M. Y ; Capanema, E. A ; Auer, M ; Adams, P. D ; Chiang, V. L ; Schuck, P. James
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5Cell wall
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7Cell walls
8Cellulose
9complex mixtures
10Ecology
11Fibers
12food
13Forestry
14Foundries
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19Imaging
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43RAPID COMMUNICATION
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45technology
46Tension wood
47Transgenic
48Transgenic plants
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atitleLabel-free in situ imaging of lignification in the cell wall of low lignin transgenic Populus trichocarpa
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abstractChemical imaging by confocal Raman microscopy has been used for the visualization of the cellulose and lignin distribution in wood cell walls. Lignin reduction in wood can be achieved by, for example, transgenic suppression of a monolignol biosynthesis gene encoding 4-coumarate-CoA ligase (4CL). Here, we use confocal Raman microscopy to compare lignification in wild type and lignin-reduced 4CL transgenic Populus trichocarpa stem wood with spatial resolution that is sub-μm. Analyzing the lignin Raman bands in the spectral region between 1,600 and 1,700 cm-1, differences in lignin signal intensity and localization are mapped in situ. Transgenic reduction of lignin is particularly pronounced in the S2 wall layer of fibers, suggesting that such transgenic approach may help overcome cell wall recalcitrance to wood saccharification. Spatial heterogeneity in the lignin composition, in particular with regard to ethylenic residues, is observed in both samples.
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pmid19526248
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