schliessen

Filtern

 

Bibliotheken

Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging

Intravital multiphoton microscopy has provided powerful mechanistic insights into health and disease and has become a common instrument in the modern biological laboratory. The requisite high numerical aperture and exogenous contrast agents that enable multiphoton microscopy, however, limit the abil... Full description

Journal Title: Nature medicine 2009-10, Vol.15 (10), p.1219-1223
Main Author: Jain, Rakesh K
Other Authors: Bouma, Brett E , Vakoc, Benjamin J , Lanning, Ryan M , Tyrrell, James A , Padera, Timothy P , Bartlett, Lisa A , Stylianopoulos, Triantafyllos , Munn, Lance L , Tearney, Guillermo J , Fukumura, Dai
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: United States: Nature Publishing Group
ID: ISSN: 1078-8956
Link: https://www.ncbi.nlm.nih.gov/pubmed/19749772
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2759417
title: Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging
format: Article
creator:
  • Jain, Rakesh K
  • Bouma, Brett E
  • Vakoc, Benjamin J
  • Lanning, Ryan M
  • Tyrrell, James A
  • Padera, Timothy P
  • Bartlett, Lisa A
  • Stylianopoulos, Triantafyllos
  • Munn, Lance L
  • Tearney, Guillermo J
  • Fukumura, Dai
subjects:
  • angiogenesis
  • animal cell
  • animal model
  • animal tissue
  • Animals
  • Article
  • breast carcinoma
  • Cancer
  • cancer tissue
  • Care and treatment
  • contrast enhancement
  • controlled study
  • Diagnosis
  • Diagnostic Imaging
  • Diagnostic Imaging - methods
  • diagnostic value
  • Dimensional
  • diphtheria toxin
  • drug cytotoxicity
  • Equipment Design
  • Equipment Design - methods
  • Experimental
  • Female
  • Fluorescein Angiography
  • Fluorescein Angiography - methods
  • Glioblastoma
  • Glioblastoma - blood supply
  • Health aspects
  • Heterologous
  • histopathology
  • human
  • human cell
  • Humans
  • image analysis
  • Image Enhancement
  • Image Enhancement - methods
  • image processing
  • Imaging
  • Imaging, Three-Dimensional - methods
  • lymphatic system
  • Lymphography
  • male
  • Mammary Neoplasms
  • Mammary Neoplasms, Experimental - pathology
  • Medical imaging
  • Methods
  • Mice
  • microenvironment
  • Microscope and microscopy
  • Microscopy
  • Microscopy - methods
  • Microvessels
  • Microvessels - pathology
  • monoclonal antibody DC101
  • mouse
  • Neoplasms
  • Neoplasms - blood supply
  • Neoplasms - pathology
  • nonhuman
  • optical frequency domaing imaging
  • outcome assessment
  • priority journal
  • Research
  • Three
  • three dimensional imaging
  • Time Factors
  • tissue structure
  • Transplantation
  • Transplantation, Heterologous
  • treatment response
  • tumor vascularization
  • Tumors
  • Usage
ispartof: Nature medicine, 2009-10, Vol.15 (10), p.1219-1223
description: Intravital multiphoton microscopy has provided powerful mechanistic insights into health and disease and has become a common instrument in the modern biological laboratory. The requisite high numerical aperture and exogenous contrast agents that enable multiphoton microscopy, however, limit the ability to investigate substantial tissue volumes or to probe dynamic changes repeatedly over prolonged periods. Here we introduce optical frequency domain imaging (OFDI) as an intravital microscopy that circumvents the technical limitations of multiphoton microscopy and, as a result, provides unprecedented access to previously unexplored, crucial aspects of tissue biology. Using unique OFDI-based approaches and entirely intrinsic mechanisms of contrast, we present rapid and repeated measurements of tumor angiogenesis, lymphangiogenesis, tissue viability and both vascular and cellular responses to therapy, thereby demonstrating the potential of OFDI to facilitate the exploration of physiological and pathological processes and the evaluation of treatment strategies.
language: eng
source:
identifier: ISSN: 1078-8956
fulltext: no_fulltext
issn:
  • 1078-8956
  • 1546-170X
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.7361321
LOCALfalse
PrimoNMBib
record
control
sourceidgale_pubme
recordidTN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2759417
sourceformatXML
sourcesystemPC
galeidA209904978
sourcerecordidA209904978
originalsourceidFETCH-LOGICAL-1699t-c74fbbb80ca3d0e733d9c1c260712793893b20e866afb23c4d4e5f6c7103ff910
addsrcrecordideNqNk19rFDEUxQdRbF3FT6AMPlR8mJpk8vdFKEVtoVDQKr6FTCbZTZlJ1klmsd_eu2zVbvGhzMMMye-euTn3pKpeYnSMUSvfx_EYK4EfVYeYUd5ggX48hm8kZCMV4wfVs5yvEUItYuppdQAsVUKQwypcrSbnmj6MLuaQohnqMdgpZZvWN3XydVm5usxjmnbrLm7ClCLQpQ6x3oRNqucc4rJO6xIslPvJ_ZxdtDd1n0YDTBjNEoDn1RNvhuxe3L4X1bdPH69Oz5qLy8_npycXDeZKlcYK6ruuk8iatkdOtG2vLLaEI4GJUK1UbUeQk5wb35HW0p465rkV4IP3CqNF9WGnu5670fUWOp3MoNcT9DHd6GSC3t-JYaWXaaOJYIqCc4vqfCeQ1i6aMLm92j66olOvKZVMM06U5AypnnlomwnmkaFcIdwZShQHrbMHaBEuNJzYdMpIa1jnjOoMaZn1UnBrPPGKgtTR7bmmBAbnoseQrRsGE12as-aCS0Hl1oA398DrNE8w2awJaTGmBBpcVMc7aGkGp0P0Cbyw8PQO5pyi8wHWTwhSCkFUJBS82ysAprhfZWnmnPX51y8PZy-_77NHd9iVM0NZ5TTMBcKY98G3O3Cbzjw5_9dKjPT2Eug46u0lAPL13QT8425TD0BzT8qGYrZ_BBPC8B_BVzs-mjLfmeGf_d-8hBlf
sourcetypeOpen Access Repository
isCDItrue
recordtypearticle
pqid223114269
display
typearticle
titleThree-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging
creatorJain, Rakesh K ; Bouma, Brett E ; Vakoc, Benjamin J ; Lanning, Ryan M ; Tyrrell, James A ; Padera, Timothy P ; Bartlett, Lisa A ; Stylianopoulos, Triantafyllos ; Munn, Lance L ; Tearney, Guillermo J ; Fukumura, Dai
creatorcontribJain, Rakesh K ; Bouma, Brett E ; Vakoc, Benjamin J ; Lanning, Ryan M ; Tyrrell, James A ; Padera, Timothy P ; Bartlett, Lisa A ; Stylianopoulos, Triantafyllos ; Munn, Lance L ; Tearney, Guillermo J ; Fukumura, Dai
descriptionIntravital multiphoton microscopy has provided powerful mechanistic insights into health and disease and has become a common instrument in the modern biological laboratory. The requisite high numerical aperture and exogenous contrast agents that enable multiphoton microscopy, however, limit the ability to investigate substantial tissue volumes or to probe dynamic changes repeatedly over prolonged periods. Here we introduce optical frequency domain imaging (OFDI) as an intravital microscopy that circumvents the technical limitations of multiphoton microscopy and, as a result, provides unprecedented access to previously unexplored, crucial aspects of tissue biology. Using unique OFDI-based approaches and entirely intrinsic mechanisms of contrast, we present rapid and repeated measurements of tumor angiogenesis, lymphangiogenesis, tissue viability and both vascular and cellular responses to therapy, thereby demonstrating the potential of OFDI to facilitate the exploration of physiological and pathological processes and the evaluation of treatment strategies.
identifier
0ISSN: 1078-8956
1EISSN: 1546-170X
2DOI: 10.1038/nm.1971
3PMID: 19749772
languageeng
publisherUnited States: Nature Publishing Group
subjectangiogenesis ; animal cell ; animal model ; animal tissue ; Animals ; Article ; breast carcinoma ; Cancer ; cancer tissue ; Care and treatment ; contrast enhancement ; controlled study ; Diagnosis ; Diagnostic Imaging ; Diagnostic Imaging - methods ; diagnostic value ; Dimensional ; diphtheria toxin ; drug cytotoxicity ; Equipment Design ; Equipment Design - methods ; Experimental ; Female ; Fluorescein Angiography ; Fluorescein Angiography - methods ; Glioblastoma ; Glioblastoma - blood supply ; Health aspects ; Heterologous ; histopathology ; human ; human cell ; Humans ; image analysis ; Image Enhancement ; Image Enhancement - methods ; image processing ; Imaging ; Imaging, Three-Dimensional - methods ; lymphatic system ; Lymphography ; male ; Mammary Neoplasms ; Mammary Neoplasms, Experimental - pathology ; Medical imaging ; Methods ; Mice ; microenvironment ; Microscope and microscopy ; Microscopy ; Microscopy - methods ; Microvessels ; Microvessels - pathology ; monoclonal antibody DC101 ; mouse ; Neoplasms ; Neoplasms - blood supply ; Neoplasms - pathology ; nonhuman ; optical frequency domaing imaging ; outcome assessment ; priority journal ; Research ; Three ; three dimensional imaging ; Time Factors ; tissue structure ; Transplantation ; Transplantation, Heterologous ; treatment response ; tumor vascularization ; Tumors ; Usage
ispartofNature medicine, 2009-10, Vol.15 (10), p.1219-1223
rights
0COPYRIGHT 2009 Nature Publishing Group
1Copyright Nature Publishing Group Oct 2009
lds50peer_reviewed
oafree_for_read
citedbyFETCH-LOGICAL-1699t-c74fbbb80ca3d0e733d9c1c260712793893b20e866afb23c4d4e5f6c7103ff910
citesFETCH-LOGICAL-1699t-c74fbbb80ca3d0e733d9c1c260712793893b20e866afb23c4d4e5f6c7103ff910
links
openurl$$Topenurl_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19749772$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Jain, Rakesh K
1Bouma, Brett E
2Vakoc, Benjamin J
3Lanning, Ryan M
4Tyrrell, James A
5Padera, Timothy P
6Bartlett, Lisa A
7Stylianopoulos, Triantafyllos
8Munn, Lance L
9Tearney, Guillermo J
10Fukumura, Dai
title
0Three-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging
1Nature medicine
addtitleNat Med
descriptionIntravital multiphoton microscopy has provided powerful mechanistic insights into health and disease and has become a common instrument in the modern biological laboratory. The requisite high numerical aperture and exogenous contrast agents that enable multiphoton microscopy, however, limit the ability to investigate substantial tissue volumes or to probe dynamic changes repeatedly over prolonged periods. Here we introduce optical frequency domain imaging (OFDI) as an intravital microscopy that circumvents the technical limitations of multiphoton microscopy and, as a result, provides unprecedented access to previously unexplored, crucial aspects of tissue biology. Using unique OFDI-based approaches and entirely intrinsic mechanisms of contrast, we present rapid and repeated measurements of tumor angiogenesis, lymphangiogenesis, tissue viability and both vascular and cellular responses to therapy, thereby demonstrating the potential of OFDI to facilitate the exploration of physiological and pathological processes and the evaluation of treatment strategies.
subject
0angiogenesis
1animal cell
2animal model
3animal tissue
4Animals
5Article
6breast carcinoma
7Cancer
8cancer tissue
9Care and treatment
10contrast enhancement
11controlled study
12Diagnosis
13Diagnostic Imaging
14Diagnostic Imaging - methods
15diagnostic value
16Dimensional
17diphtheria toxin
18drug cytotoxicity
19Equipment Design
20Equipment Design - methods
21Experimental
22Female
23Fluorescein Angiography
24Fluorescein Angiography - methods
25Glioblastoma
26Glioblastoma - blood supply
27Health aspects
28Heterologous
29histopathology
30human
31human cell
32Humans
33image analysis
34Image Enhancement
35Image Enhancement - methods
36image processing
37Imaging
38Imaging, Three-Dimensional - methods
39lymphatic system
40Lymphography
41male
42Mammary Neoplasms
43Mammary Neoplasms, Experimental - pathology
44Medical imaging
45Methods
46Mice
47microenvironment
48Microscope and microscopy
49Microscopy
50Microscopy - methods
51Microvessels
52Microvessels - pathology
53monoclonal antibody DC101
54mouse
55Neoplasms
56Neoplasms - blood supply
57Neoplasms - pathology
58nonhuman
59optical frequency domaing imaging
60outcome assessment
61priority journal
62Research
63Three
64three dimensional imaging
65Time Factors
66tissue structure
67Transplantation
68Transplantation, Heterologous
69treatment response
70tumor vascularization
71Tumors
72Usage
issn
01078-8956
11546-170X
fulltextfalse
rsrctypearticle
creationdate2009
recordtypearticle
recordideNqNk19rFDEUxQdRbF3FT6AMPlR8mJpk8vdFKEVtoVDQKr6FTCbZTZlJ1klmsd_eu2zVbvGhzMMMye-euTn3pKpeYnSMUSvfx_EYK4EfVYeYUd5ggX48hm8kZCMV4wfVs5yvEUItYuppdQAsVUKQwypcrSbnmj6MLuaQohnqMdgpZZvWN3XydVm5usxjmnbrLm7ClCLQpQ6x3oRNqucc4rJO6xIslPvJ_ZxdtDd1n0YDTBjNEoDn1RNvhuxe3L4X1bdPH69Oz5qLy8_npycXDeZKlcYK6ruuk8iatkdOtG2vLLaEI4GJUK1UbUeQk5wb35HW0p465rkV4IP3CqNF9WGnu5670fUWOp3MoNcT9DHd6GSC3t-JYaWXaaOJYIqCc4vqfCeQ1i6aMLm92j66olOvKZVMM06U5AypnnlomwnmkaFcIdwZShQHrbMHaBEuNJzYdMpIa1jnjOoMaZn1UnBrPPGKgtTR7bmmBAbnoseQrRsGE12as-aCS0Hl1oA398DrNE8w2awJaTGmBBpcVMc7aGkGp0P0Cbyw8PQO5pyi8wHWTwhSCkFUJBS82ysAprhfZWnmnPX51y8PZy-_77NHd9iVM0NZ5TTMBcKY98G3O3Cbzjw5_9dKjPT2Eug46u0lAPL13QT8425TD0BzT8qGYrZ_BBPC8B_BVzs-mjLfmeGf_d-8hBlf
startdate200910
enddate200910
creator
0Jain, Rakesh K
1Bouma, Brett E
2Vakoc, Benjamin J
3Lanning, Ryan M
4Tyrrell, James A
5Padera, Timothy P
6Bartlett, Lisa A
7Stylianopoulos, Triantafyllos
8Munn, Lance L
9Tearney, Guillermo J
10Fukumura, Dai
generalNature Publishing Group
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
83V.
97QG
107QL
117QP
127QR
137T5
147TK
157TM
167TO
177U7
187U9
197X7
207XB
2188A
2288E
2388I
248AO
258FD
268FE
278FH
288FI
298FJ
308FK
318G5
32ABUWG
33AZQEC
34BBNVY
35BENPR
36BHPHI
37C1K
38DWQXO
39FR3
40FYUFA
41GHDGH
42GNUQQ
43GUQSH
44H94
45HCIFZ
46K9.
47LK8
48M0S
49M1P
50M2O
51M2P
52M7N
53M7P
54MBDVC
55P64
56PADUT
57PQEST
58PQQKQ
59PQUKI
60PRINS
61Q9U
62RC3
637X8
64BOBZL
65CLFQK
665PM
sort
creationdate200910
titleThree-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging
authorJain, Rakesh K ; Bouma, Brett E ; Vakoc, Benjamin J ; Lanning, Ryan M ; Tyrrell, James A ; Padera, Timothy P ; Bartlett, Lisa A ; Stylianopoulos, Triantafyllos ; Munn, Lance L ; Tearney, Guillermo J ; Fukumura, Dai
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1699t-c74fbbb80ca3d0e733d9c1c260712793893b20e866afb23c4d4e5f6c7103ff910
rsrctypearticles
prefilterarticles
languageeng
creationdate2009
topic
0angiogenesis
1animal cell
2animal model
3animal tissue
4Animals
5Article
6breast carcinoma
7Cancer
8cancer tissue
9Care and treatment
10contrast enhancement
11controlled study
12Diagnosis
13Diagnostic Imaging
14Diagnostic Imaging - methods
15diagnostic value
16Dimensional
17diphtheria toxin
18drug cytotoxicity
19Equipment Design
20Equipment Design - methods
21Experimental
22Female
23Fluorescein Angiography
24Fluorescein Angiography - methods
25Glioblastoma
26Glioblastoma - blood supply
27Health aspects
28Heterologous
29histopathology
30human
31human cell
32Humans
33image analysis
34Image Enhancement
35Image Enhancement - methods
36image processing
37Imaging
38Imaging, Three-Dimensional - methods
39lymphatic system
40Lymphography
41male
42Mammary Neoplasms
43Mammary Neoplasms, Experimental - pathology
44Medical imaging
45Methods
46Mice
47microenvironment
48Microscope and microscopy
49Microscopy
50Microscopy - methods
51Microvessels
52Microvessels - pathology
53monoclonal antibody DC101
54mouse
55Neoplasms
56Neoplasms - blood supply
57Neoplasms - pathology
58nonhuman
59optical frequency domaing imaging
60outcome assessment
61priority journal
62Research
63Three
64three dimensional imaging
65Time Factors
66tissue structure
67Transplantation
68Transplantation, Heterologous
69treatment response
70tumor vascularization
71Tumors
72Usage
toplevelpeer_reviewed
creatorcontrib
0Jain, Rakesh K
1Bouma, Brett E
2Vakoc, Benjamin J
3Lanning, Ryan M
4Tyrrell, James A
5Padera, Timothy P
6Bartlett, Lisa A
7Stylianopoulos, Triantafyllos
8Munn, Lance L
9Tearney, Guillermo J
10Fukumura, Dai
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7ProQuest Central (Corporate)
8Animal Behavior Abstracts
9Bacteriology Abstracts (Microbiology B)
10Calcium & Calcified Tissue Abstracts
11Chemoreception Abstracts
12Immunology Abstracts
13Neurosciences Abstracts
14Nucleic Acids Abstracts
15Oncogenes and Growth Factors Abstracts
16Toxicology Abstracts
17Virology and AIDS Abstracts
18Health & Medical Collection
19ProQuest Central (purchase pre-March 2016)
20Biology Database (Alumni Edition)
21Medical Database (Alumni Edition)
22Science Database (Alumni Edition)
23ProQuest Pharma Collection
24Technology Research Database
25ProQuest SciTech Collection
26ProQuest Natural Science Collection
27Hospital Premium Collection
28Hospital Premium Collection (Alumni Edition)
29ProQuest Central (Alumni) (purchase pre-March 2016)
30Research Library (Alumni Edition)
31ProQuest Central (Alumni Edition)
32ProQuest Central Essentials
33Biological Science Collection
34ProQuest Central
35Natural Science Collection
36Environmental Sciences and Pollution Management
37ProQuest Central Korea
38Engineering Research Database
39Health Research Premium Collection
40Health Research Premium Collection (Alumni)
41ProQuest Central Student
42Research Library Prep
43AIDS and Cancer Research Abstracts
44SciTech Premium Collection
45ProQuest Health & Medical Complete (Alumni)
46ProQuest Biological Science Collection
47Health & Medical Collection (Alumni Edition)
48Medical Database
49Research Library
50Science Database
51Algology Mycology and Protozoology Abstracts (Microbiology C)
52Biological Science Database
53Research Library (Corporate)
54Biotechnology and BioEngineering Abstracts
55Research Library China
56ProQuest One Academic Eastern Edition
57ProQuest One Academic
58ProQuest One Academic UKI Edition
59ProQuest Central China
60ProQuest Central Basic
61Genetics Abstracts
62MEDLINE - Academic
63OpenAIRE (Open Access)
64OpenAIRE
65PubMed Central (Full Participant titles)
jtitleNature medicine
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Jain, Rakesh K
1Bouma, Brett E
2Vakoc, Benjamin J
3Lanning, Ryan M
4Tyrrell, James A
5Padera, Timothy P
6Bartlett, Lisa A
7Stylianopoulos, Triantafyllos
8Munn, Lance L
9Tearney, Guillermo J
10Fukumura, Dai
formatjournal
genrearticle
ristypeJOUR
atitleThree-dimensional microscopy of the tumor microenvironment in vivo using optical frequency domain imaging
jtitleNature medicine
addtitleNat Med
date2009-10
risdate2009
volume15
issue10
spage1219
epage1223
pages1219-1223
issn1078-8956
eissn1546-170X
notesAuthors contributed equally to this work
abstractIntravital multiphoton microscopy has provided powerful mechanistic insights into health and disease and has become a common instrument in the modern biological laboratory. The requisite high numerical aperture and exogenous contrast agents that enable multiphoton microscopy, however, limit the ability to investigate substantial tissue volumes or to probe dynamic changes repeatedly over prolonged periods. Here we introduce optical frequency domain imaging (OFDI) as an intravital microscopy that circumvents the technical limitations of multiphoton microscopy and, as a result, provides unprecedented access to previously unexplored, crucial aspects of tissue biology. Using unique OFDI-based approaches and entirely intrinsic mechanisms of contrast, we present rapid and repeated measurements of tumor angiogenesis, lymphangiogenesis, tissue viability and both vascular and cellular responses to therapy, thereby demonstrating the potential of OFDI to facilitate the exploration of physiological and pathological processes and the evaluation of treatment strategies.
copUnited States
pubNature Publishing Group
pmid19749772
doi10.1038/nm.1971
tpages6
oafree_for_read