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Modulation of the Proteome of Peripheral Blood Mononuclear Cells from HIV-1-Infected Patients by Drugs of Abuse

Introduction We used proteomic analyses to assess how drug abuse modulates immunologic responses to infections with the human immunodeficiency virus type 1 (HIV-1). Methods Two-dimensional difference gel electrophoresis was utilized to determine changes in the proteome of peripheral blood mononuclea... Full description

Journal Title: Journal of clinical immunology 2009-06-20, Vol.29 (5), p.646-656
Main Author: Reynolds, Jessica L
Other Authors: Mahajan, Supriya D , Aalinkeel, Ravikunar , Nair, Bindukumar , Sykes, Donald E , Agosto-Mujica, Anardi , Hsiao, Chiu Bin , Schwartz, Stanley A
Format: Electronic Article Electronic Article
Language: English
Subjects:
MS
Publisher: Boston: Springer US
ID: ISSN: 0271-9142
Link: https://www.ncbi.nlm.nih.gov/pubmed/19543960
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title: Modulation of the Proteome of Peripheral Blood Mononuclear Cells from HIV-1-Infected Patients by Drugs of Abuse
format: Article
creator:
  • Reynolds, Jessica L
  • Mahajan, Supriya D
  • Aalinkeel, Ravikunar
  • Nair, Bindukumar
  • Sykes, Donald E
  • Agosto-Mujica, Anardi
  • Hsiao, Chiu Bin
  • Schwartz, Stanley A
subjects:
  • Article
  • Biomedical and Life Sciences
  • Biomedicine
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Cocaine
  • Cocaine - pharmacology
  • Data processing
  • Development and progression
  • difference gel electrophoresis (DIGE)
  • Drug abuse
  • Drug therapy
  • Electrophoresis, Gel, Two-Dimensional
  • Gel electrophoresis
  • Gene expression
  • Gene Expression Regulation
  • Health aspects
  • high performance liquid chromatography-tandem mass spectrometry (HPLC-MS
  • HIV (Viruses)
  • HIV infection
  • HIV Infections - blood
  • HIV Infections - genetics
  • HIV Infections - immunology
  • HIV Infections - metabolism
  • HIV Infections - pathology
  • HIV-1 - immunology
  • HIV-1 - pathogenicity
  • Human immunodeficiency virus 1
  • human immunodeficiency virus type 1 (HIV-1)
  • Humans
  • Illegal drugs
  • Immunology
  • Infection
  • Infectious Diseases
  • Internal Medicine
  • Leukocytes, Mononuclear - drug effects
  • Leukocytes, Mononuclear - metabolism
  • Leukocytes, Mononuclear - pathology
  • Leukocytes, Mononuclear - virology
  • Lymphocytes B
  • Lymphocytes T
  • Mass spectrometry
  • Medical Microbiology
  • Methamphetamine
  • Methamphetamine - pharmacology
  • Molecular modelling
  • Monocytes
  • MS
  • Peripheral blood mononuclear cells
  • peripheral blood mononuclear cells (PBMC)
  • Polymerase Chain Reaction
  • Proteins
  • Proteome - genetics
  • Proteome - immunology
  • Proteome - metabolism
  • proteomics
  • Substance-Related Disorders - immunology
  • T cells
ispartof: Journal of clinical immunology, 2009-06-20, Vol.29 (5), p.646-656
description: Introduction We used proteomic analyses to assess how drug abuse modulates immunologic responses to infections with the human immunodeficiency virus type 1 (HIV-1). Methods Two-dimensional difference gel electrophoresis was utilized to determine changes in the proteome of peripheral blood mononuclear cells (PBMC) isolated from HIV-1-positive donors that occurred after treatment with cocaine or methamphetamine. Both drugs differentially regulated the expression of several functional classes of proteins. We further isolated specific subpopulations of PBMC to determine which subpopulations were selectively affected by treatment with drugs of abuse. Monocytes, B cells, and T cells were positively or negatively selected from PBMC isolated from HIV-1-positive donors. Results Our results demonstrate that cocaine and methamphetamine modulate gene expression primarily in monocytes and T cells, the primary targets of HIV-1 infection. Proteomic data were validated with quantitative, real-time polymerase chain reaction. These studies elucidate the molecular mechanisms underlying the effects of drugs of abuse on HIV-1 infections. Several functionally relevant classes of proteins were identified as potential mediators of HIV-1 pathogenesis and disease progression associated with drug abuse.
language: eng
source:
identifier: ISSN: 0271-9142
fulltext: no_fulltext
issn:
  • 0271-9142
  • 1573-2592
url: Link


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titleModulation of the Proteome of Peripheral Blood Mononuclear Cells from HIV-1-Infected Patients by Drugs of Abuse
creatorReynolds, Jessica L ; Mahajan, Supriya D ; Aalinkeel, Ravikunar ; Nair, Bindukumar ; Sykes, Donald E ; Agosto-Mujica, Anardi ; Hsiao, Chiu Bin ; Schwartz, Stanley A
creatorcontribReynolds, Jessica L ; Mahajan, Supriya D ; Aalinkeel, Ravikunar ; Nair, Bindukumar ; Sykes, Donald E ; Agosto-Mujica, Anardi ; Hsiao, Chiu Bin ; Schwartz, Stanley A
descriptionIntroduction We used proteomic analyses to assess how drug abuse modulates immunologic responses to infections with the human immunodeficiency virus type 1 (HIV-1). Methods Two-dimensional difference gel electrophoresis was utilized to determine changes in the proteome of peripheral blood mononuclear cells (PBMC) isolated from HIV-1-positive donors that occurred after treatment with cocaine or methamphetamine. Both drugs differentially regulated the expression of several functional classes of proteins. We further isolated specific subpopulations of PBMC to determine which subpopulations were selectively affected by treatment with drugs of abuse. Monocytes, B cells, and T cells were positively or negatively selected from PBMC isolated from HIV-1-positive donors. Results Our results demonstrate that cocaine and methamphetamine modulate gene expression primarily in monocytes and T cells, the primary targets of HIV-1 infection. Proteomic data were validated with quantitative, real-time polymerase chain reaction. These studies elucidate the molecular mechanisms underlying the effects of drugs of abuse on HIV-1 infections. Several functionally relevant classes of proteins were identified as potential mediators of HIV-1 pathogenesis and disease progression associated with drug abuse.
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subjectArticle ; Biomedical and Life Sciences ; Biomedicine ; Cells, Cultured ; Chromatography, High Pressure Liquid ; Cocaine ; Cocaine - pharmacology ; Data processing ; Development and progression ; difference gel electrophoresis (DIGE) ; Drug abuse ; Drug therapy ; Electrophoresis, Gel, Two-Dimensional ; Gel electrophoresis ; Gene expression ; Gene Expression Regulation ; Health aspects ; high performance liquid chromatography-tandem mass spectrometry (HPLC-MS ; HIV (Viruses) ; HIV infection ; HIV Infections - blood ; HIV Infections - genetics ; HIV Infections - immunology ; HIV Infections - metabolism ; HIV Infections - pathology ; HIV-1 - immunology ; HIV-1 - pathogenicity ; Human immunodeficiency virus 1 ; human immunodeficiency virus type 1 (HIV-1) ; Humans ; Illegal drugs ; Immunology ; Infection ; Infectious Diseases ; Internal Medicine ; Leukocytes, Mononuclear - drug effects ; Leukocytes, Mononuclear - metabolism ; Leukocytes, Mononuclear - pathology ; Leukocytes, Mononuclear - virology ; Lymphocytes B ; Lymphocytes T ; Mass spectrometry ; Medical Microbiology ; Methamphetamine ; Methamphetamine - pharmacology ; Molecular modelling ; Monocytes ; MS ; Peripheral blood mononuclear cells ; peripheral blood mononuclear cells (PBMC) ; Polymerase Chain Reaction ; Proteins ; Proteome - genetics ; Proteome - immunology ; Proteome - metabolism ; proteomics ; Substance-Related Disorders - immunology ; T cells
ispartofJournal of clinical immunology, 2009-06-20, Vol.29 (5), p.646-656
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1Mahajan, Supriya D
2Aalinkeel, Ravikunar
3Nair, Bindukumar
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5Agosto-Mujica, Anardi
6Hsiao, Chiu Bin
7Schwartz, Stanley A
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descriptionIntroduction We used proteomic analyses to assess how drug abuse modulates immunologic responses to infections with the human immunodeficiency virus type 1 (HIV-1). Methods Two-dimensional difference gel electrophoresis was utilized to determine changes in the proteome of peripheral blood mononuclear cells (PBMC) isolated from HIV-1-positive donors that occurred after treatment with cocaine or methamphetamine. Both drugs differentially regulated the expression of several functional classes of proteins. We further isolated specific subpopulations of PBMC to determine which subpopulations were selectively affected by treatment with drugs of abuse. Monocytes, B cells, and T cells were positively or negatively selected from PBMC isolated from HIV-1-positive donors. Results Our results demonstrate that cocaine and methamphetamine modulate gene expression primarily in monocytes and T cells, the primary targets of HIV-1 infection. Proteomic data were validated with quantitative, real-time polymerase chain reaction. These studies elucidate the molecular mechanisms underlying the effects of drugs of abuse on HIV-1 infections. Several functionally relevant classes of proteins were identified as potential mediators of HIV-1 pathogenesis and disease progression associated with drug abuse.
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14Gene expression
15Gene Expression Regulation
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18HIV (Viruses)
19HIV infection
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21HIV Infections - genetics
22HIV Infections - immunology
23HIV Infections - metabolism
24HIV Infections - pathology
25HIV-1 - immunology
26HIV-1 - pathogenicity
27Human immunodeficiency virus 1
28human immunodeficiency virus type 1 (HIV-1)
29Humans
30Illegal drugs
31Immunology
32Infection
33Infectious Diseases
34Internal Medicine
35Leukocytes, Mononuclear - drug effects
36Leukocytes, Mononuclear - metabolism
37Leukocytes, Mononuclear - pathology
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40Lymphocytes T
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42Medical Microbiology
43Methamphetamine
44Methamphetamine - pharmacology
45Molecular modelling
46Monocytes
47MS
48Peripheral blood mononuclear cells
49peripheral blood mononuclear cells (PBMC)
50Polymerase Chain Reaction
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52Proteome - genetics
53Proteome - immunology
54Proteome - metabolism
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56Substance-Related Disorders - immunology
57T cells
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titleModulation of the Proteome of Peripheral Blood Mononuclear Cells from HIV-1-Infected Patients by Drugs of Abuse
authorReynolds, Jessica L ; Mahajan, Supriya D ; Aalinkeel, Ravikunar ; Nair, Bindukumar ; Sykes, Donald E ; Agosto-Mujica, Anardi ; Hsiao, Chiu Bin ; Schwartz, Stanley A
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15Gene Expression Regulation
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21HIV Infections - genetics
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36Leukocytes, Mononuclear - metabolism
37Leukocytes, Mononuclear - pathology
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40Lymphocytes T
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42Medical Microbiology
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abstractIntroduction We used proteomic analyses to assess how drug abuse modulates immunologic responses to infections with the human immunodeficiency virus type 1 (HIV-1). Methods Two-dimensional difference gel electrophoresis was utilized to determine changes in the proteome of peripheral blood mononuclear cells (PBMC) isolated from HIV-1-positive donors that occurred after treatment with cocaine or methamphetamine. Both drugs differentially regulated the expression of several functional classes of proteins. We further isolated specific subpopulations of PBMC to determine which subpopulations were selectively affected by treatment with drugs of abuse. Monocytes, B cells, and T cells were positively or negatively selected from PBMC isolated from HIV-1-positive donors. Results Our results demonstrate that cocaine and methamphetamine modulate gene expression primarily in monocytes and T cells, the primary targets of HIV-1 infection. Proteomic data were validated with quantitative, real-time polymerase chain reaction. These studies elucidate the molecular mechanisms underlying the effects of drugs of abuse on HIV-1 infections. Several functionally relevant classes of proteins were identified as potential mediators of HIV-1 pathogenesis and disease progression associated with drug abuse.
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