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Evaluation of the Dengue NS1 Ag Strip® for Detection of Dengue Virus Antigen in Aedes aegypti (Diptera: Culicidae)

Dengue fever is currently one of the most important mosquito-borne diseases that affect humans. With neither vaccines nor treatment available, prevention of the disease relies heavily on surveillance and control of mosquito vectors. In the present study, we have evaluated and showed the potential us... Full description

Journal Title: Vector borne and zoonotic diseases (Larchmont N.Y.), 2011, Vol.11 (6), p.789-792
Main Author: Tan, Cheong-Huat
Other Authors: Wong, Pei-Sze Jeslyn , Li, Mei-Zhi Irene , Vythilingam, Indra , Ng, Lee-Ching
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: United States: Mary Ann Liebert, Inc
ID: ISSN: 1530-3667
Link: https://www.ncbi.nlm.nih.gov/pubmed/21395416
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recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3115461
title: Evaluation of the Dengue NS1 Ag Strip® for Detection of Dengue Virus Antigen in Aedes aegypti (Diptera: Culicidae)
format: Article
creator:
  • Tan, Cheong-Huat
  • Wong, Pei-Sze Jeslyn
  • Li, Mei-Zhi Irene
  • Vythilingam, Indra
  • Ng, Lee-Ching
subjects:
  • Aedes - immunology
  • Aedes - virology
  • Aedes aegypti
  • Animals
  • Antigens
  • Dengue
  • Dengue NS1 Ag Strip
  • Dengue virus
  • Dengue Virus - immunology
  • Diagnosis
  • Disease transmission
  • fungi
  • Health aspects
  • NS1 protein
  • Rapid test kit
  • Reagent Kits, Diagnostic
  • Reagent Strips
  • Real-time RT-PCR
  • Reproducibility of Results
  • Reverse Transcriptase Polymerase Chain Reaction
  • Risk factors
  • Sensitivity and Specificity
  • Short Communication
  • Time
  • Viral Nonstructural Proteins - immunology
  • virus diseases
  • viruses
ispartof: Vector borne and zoonotic diseases (Larchmont, N.Y.), 2011, Vol.11 (6), p.789-792
description: Dengue fever is currently one of the most important mosquito-borne diseases that affect humans. With neither vaccines nor treatment available, prevention of the disease relies heavily on surveillance and control of mosquito vectors. In the present study, we have evaluated and showed the potential use of the Dengue NS1 Ag Strip ® for the detection of dengue virus (DENV) in Aedes aegypti . Initial results showed that the sensitivity of the test kit in detecting DENV in wild-caught mosquitoes is comparable to that of real-time reverse transcriptase–polymerase chain reaction. The detection of naturally infected Ae. aegypti with the NS1 rapid test kit in our dengue cluster investigation further illustrates its potential use for surveillance of DENV in wild mosquito populations. The kit can easily be used in a simple field station, and minimal training is required. The results can be obtained in less than an hour. Employment of the kit in the field could help guide mosquito control operations in the prioritization of resources in controlling the transmission of DENV. In this study the potential of the kit for field surveillance of infected dengue vectors, which are epidemiologically important, has been demonstrated.
language: eng
source:
identifier: ISSN: 1530-3667
fulltext: no_fulltext
issn:
  • 1530-3667
  • 1557-7759
url: Link


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creatorTan, Cheong-Huat ; Wong, Pei-Sze Jeslyn ; Li, Mei-Zhi Irene ; Vythilingam, Indra ; Ng, Lee-Ching
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descriptionDengue fever is currently one of the most important mosquito-borne diseases that affect humans. With neither vaccines nor treatment available, prevention of the disease relies heavily on surveillance and control of mosquito vectors. In the present study, we have evaluated and showed the potential use of the Dengue NS1 Ag Strip ® for the detection of dengue virus (DENV) in Aedes aegypti . Initial results showed that the sensitivity of the test kit in detecting DENV in wild-caught mosquitoes is comparable to that of real-time reverse transcriptase–polymerase chain reaction. The detection of naturally infected Ae. aegypti with the NS1 rapid test kit in our dengue cluster investigation further illustrates its potential use for surveillance of DENV in wild mosquito populations. The kit can easily be used in a simple field station, and minimal training is required. The results can be obtained in less than an hour. Employment of the kit in the field could help guide mosquito control operations in the prioritization of resources in controlling the transmission of DENV. In this study the potential of the kit for field surveillance of infected dengue vectors, which are epidemiologically important, has been demonstrated.
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subjectAedes - immunology ; Aedes - virology ; Aedes aegypti ; Animals ; Antigens ; Dengue ; Dengue NS1 Ag Strip ; Dengue virus ; Dengue Virus - immunology ; Diagnosis ; Disease transmission ; fungi ; Health aspects ; NS1 protein ; Rapid test kit ; Reagent Kits, Diagnostic ; Reagent Strips ; Real-time RT-PCR ; Reproducibility of Results ; Reverse Transcriptase Polymerase Chain Reaction ; Risk factors ; Sensitivity and Specificity ; Short Communication ; Time ; Viral Nonstructural Proteins - immunology ; virus diseases ; viruses
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abstractDengue fever is currently one of the most important mosquito-borne diseases that affect humans. With neither vaccines nor treatment available, prevention of the disease relies heavily on surveillance and control of mosquito vectors. In the present study, we have evaluated and showed the potential use of the Dengue NS1 Ag Strip ® for the detection of dengue virus (DENV) in Aedes aegypti . Initial results showed that the sensitivity of the test kit in detecting DENV in wild-caught mosquitoes is comparable to that of real-time reverse transcriptase–polymerase chain reaction. The detection of naturally infected Ae. aegypti with the NS1 rapid test kit in our dengue cluster investigation further illustrates its potential use for surveillance of DENV in wild mosquito populations. The kit can easily be used in a simple field station, and minimal training is required. The results can be obtained in less than an hour. Employment of the kit in the field could help guide mosquito control operations in the prioritization of resources in controlling the transmission of DENV. In this study the potential of the kit for field surveillance of infected dengue vectors, which are epidemiologically important, has been demonstrated.
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