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Genetic engineering of human ES and iPS cells using TALE nucleases

Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator–like effector nucleases (TALENs) for five distinct genomic loci. At all... Full description

Journal Title: Nature biotechnology 2011, Vol.29 (8), p.731-734
Main Author: Hockemeyer, Dirk
Other Authors: Wang, Haoyi , Kiani, Samira , Lai, Christine S. , Gao, Qing , Cassady, John P. , Cost, Gregory J. , Zhang, Lei , Santiago, Yolanda , Miller, Jeffrey C. , Zeitler, Bryan , Cherone, Jennifer M. , Meng, Xiangdong , Hinkley, Sarah J. , Rebar, Edward J. , Gregory, Philip D. , Urnov, Fyodor D. , Jaenisch, Rudolf
Format: Electronic Article Electronic Article
Language: English
Publisher: Nature Publishing Group
ID: ISSN: 1087-0156
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title: Genetic engineering of human ES and iPS cells using TALE nucleases
format: Article
creator:
  • Hockemeyer, Dirk
  • Wang, Haoyi
  • Kiani, Samira
  • Lai, Christine S.
  • Gao, Qing
  • Cassady, John P.
  • Cost, Gregory J.
  • Zhang, Lei
  • Santiago, Yolanda
  • Miller, Jeffrey C.
  • Zeitler, Bryan
  • Cherone, Jennifer M.
  • Meng, Xiangdong
  • Hinkley, Sarah J.
  • Rebar, Edward J.
  • Gregory, Philip D.
  • Urnov, Fyodor D.
  • Jaenisch, Rudolf
ispartof: Nature biotechnology, 2011, Vol.29 (8), p.731-734
description: Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator–like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
language: eng
source:
identifier: ISSN: 1087-0156
fulltext: no_fulltext
issn:
  • 1087-0156
  • 1546-1696
url: Link


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titleGenetic engineering of human ES and iPS cells using TALE nucleases
creatorHockemeyer, Dirk ; Wang, Haoyi ; Kiani, Samira ; Lai, Christine S. ; Gao, Qing ; Cassady, John P. ; Cost, Gregory J. ; Zhang, Lei ; Santiago, Yolanda ; Miller, Jeffrey C. ; Zeitler, Bryan ; Cherone, Jennifer M. ; Meng, Xiangdong ; Hinkley, Sarah J. ; Rebar, Edward J. ; Gregory, Philip D. ; Urnov, Fyodor D. ; Jaenisch, Rudolf
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descriptionTargeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator–like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
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descriptionTargeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator–like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
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titleGenetic engineering of human ES and iPS cells using TALE nucleases
authorHockemeyer, Dirk ; Wang, Haoyi ; Kiani, Samira ; Lai, Christine S. ; Gao, Qing ; Cassady, John P. ; Cost, Gregory J. ; Zhang, Lei ; Santiago, Yolanda ; Miller, Jeffrey C. ; Zeitler, Bryan ; Cherone, Jennifer M. ; Meng, Xiangdong ; Hinkley, Sarah J. ; Rebar, Edward J. ; Gregory, Philip D. ; Urnov, Fyodor D. ; Jaenisch, Rudolf
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9Miller, Jeffrey C.
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8Santiago, Yolanda
9Miller, Jeffrey C.
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11Cherone, Jennifer M.
12Meng, Xiangdong
13Hinkley, Sarah J.
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issn1087-0156
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abstractTargeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here we engineered transcription activator–like effector nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained human embryonic stem cell (ESC) and induced pluripotent stem cell (iPSC) clones carrying transgenic cassettes solely at the TALEN-specified location. Our data suggest that TALENs employing the specific architectures described here mediate site-specific genome modification in human pluripotent cells with similar efficiency and precision as do zinc-finger nucleases (ZFNs).
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