schliessen

Filtern

 

Bibliotheken

Gemcitabine combined with gum mastic causes potent growth inhibition and apoptosis of pancreatic cancer cells

Aim: To investigate the antiproliferative and apoptotic effects of gemcitabine combined with gum mastic and the underlying mechanisms in human pancreatic cancer cell lines. Methods: Cell proliferation and apoptosis were examined using the methyl thiazolyl tetrazolium (MTT) assay and propidium iodine... Full description

Journal Title: Acta pharmacologica Sinica 2010, Vol.31 (6), p.741-745
Main Author: Huang, Xin-yu
Other Authors: Wang, Hong-cheng , Yuan, Zhou , Li, Ang , He, Mei-lan , Ai, Kai-xing , Zheng, Qi , Qin, Huan-long
Format: Electronic Article Electronic Article
Language: English
Subjects:
Bax
Publisher: United States: Nature Publishing Group
ID: ISSN: 1671-4083
Link: https://www.ncbi.nlm.nih.gov/pubmed/20523344
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4002976
title: Gemcitabine combined with gum mastic causes potent growth inhibition and apoptosis of pancreatic cancer cells
format: Article
creator:
  • Huang, Xin-yu
  • Wang, Hong-cheng
  • Yuan, Zhou
  • Li, Ang
  • He, Mei-lan
  • Ai, Kai-xing
  • Zheng, Qi
  • Qin, Huan-long
subjects:
  • Antimetabolites, Antineoplastic - pharmacology
  • Antineoplastic Agents, Phytogenic - pharmacology
  • Antineoplastic Agents, Phytogenic - therapeutic use
  • apoptosis
  • Apoptosis - drug effects
  • Bax
  • Bax蛋白
  • Bcl-2
  • bcl-2-Associated X Protein - metabolism
  • Blotting, Western
  • Cell Line, Tumor
  • Deoxycytidine - analogs & derivatives
  • Deoxycytidine - pharmacology
  • Flow Cytometry
  • gemcitabine
  • Genes, bcl-2
  • Growth Inhibitors - therapeutic use
  • gum mastic
  • Humans
  • I-kappa B Proteins - metabolism
  • Mastic Resin
  • NF-kappa B - metabolism
  • NF-kappaB
  • Original
  • Original Article
  • pancreatic cancer
  • Pancreatic Neoplasms - drug therapy
  • Pancreatic Neoplasms - pathology
  • Resins, Plant - pharmacology
  • Resins, Plant - therapeutic use
  • Western印迹法
  • 生长抑制
  • 细胞凋亡
  • 细胞增殖
  • 胰腺癌
ispartof: Acta pharmacologica Sinica, 2010, Vol.31 (6), p.741-745
description: Aim: To investigate the antiproliferative and apoptotic effects of gemcitabine combined with gum mastic and the underlying mechanisms in human pancreatic cancer cell lines. Methods: Cell proliferation and apoptosis were examined using the methyl thiazolyl tetrazolium (MTT) assay and propidium iodine staining, respectively. The expression of Bcl-2, Bax, NF-KB p65 subunit, and IKBC( protein was measured using Western blotting. Results: Gemcitabine 0.01-100 pg/mL inhibited cell proliferation and induced apoptosis in both pancreatic cancer BxPC-3 and COLO 357 cells. Gum mastic 40 pg/mL significantly potentiated the antiproliferative and apoptotic effects of gemcitabine 10 μg/mL after 72-h treatment. When cells were treated with gemcitabine in combination with gum mastic, the IKBα level was increased, whereas NF-κB activation was blocked; the expression of Bax protein was substantially increased, but Bcl-2 protein was down-regulated. Conclusion: Gemcitabine combined with gum mastic causes potent apoptosis in pancreatic cancer cells. The combination may be an effective therapeutic strategy for pancreatic cancer.
language: eng
source:
identifier: ISSN: 1671-4083
fulltext: no_fulltext
issn:
  • 1671-4083
  • 1745-7254
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.2178056
LOCALfalse
PrimoNMBib
record
control
sourceidproquest_pubme
recordidTN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4002976
sourceformatXML
sourcesystemPC
cqvip_id34285736
sourcerecordid2049512421
originalsourceidFETCH-LOGICAL-1492t-ab0686fc122bbbeeb1cfcf12e7987d83e4634c3c75b74bfe8a4b74fdf8a736ba0
addsrcrecordideNpVkc1v1DAQxSMEoqVw4g4WV7TFX4mdCxKqoEWqxAXO1tiZ7Lps7NR2WvHf4yVL1Z481rz5-Y1f07xl9JxRoT_BnM85rbdWPmtOmZLtRvFWPq91p9hGUi1Omlc531AquGD9y-aE05YLIeVpM13i5HwB6wMSF6fDOZB7X3Zku0xkgly8Iw6WjJnMsWAoZJvife37sPPWFx8DgTAQmONcYvaZxJHMEFxCWGeDw0Qc7vf5dfNihH3GN8fzrPn17evPi6vN9Y_L7xdfrjdM9rxswNJOd6NjnFtrES1zoxsZR9VrNWiBshPSCadaq6QdUYOsxTiMGpToLNCz5vPKnRc74eCq6wR7Myc_QfpjInjztBP8zmzjnZGU8l51FXC1AuKMAXzCJ7NDwGLiYHinTC9bLbSzllsnrZAIgGD7uoF0cuh0RX04eknxdsFczE1cUqjrG9EqViNkooo-riKXYs4Jx4f3GDWHlE1N2RxSNq2s6neP13vQ_o-1Ct6vggBleWS-Qg6Mf4ijK7eLYXvrw9ZYcL9Hv0cjJNdt_Uj6F5ixvBo
sourcetypeOpen Access Repository
isCDItrue
recordtypearticle
pqid357110313
display
typearticle
titleGemcitabine combined with gum mastic causes potent growth inhibition and apoptosis of pancreatic cancer cells
creatorHuang, Xin-yu ; Wang, Hong-cheng ; Yuan, Zhou ; Li, Ang ; He, Mei-lan ; Ai, Kai-xing ; Zheng, Qi ; Qin, Huan-long
creatorcontribHuang, Xin-yu ; Wang, Hong-cheng ; Yuan, Zhou ; Li, Ang ; He, Mei-lan ; Ai, Kai-xing ; Zheng, Qi ; Qin, Huan-long
descriptionAim: To investigate the antiproliferative and apoptotic effects of gemcitabine combined with gum mastic and the underlying mechanisms in human pancreatic cancer cell lines. Methods: Cell proliferation and apoptosis were examined using the methyl thiazolyl tetrazolium (MTT) assay and propidium iodine staining, respectively. The expression of Bcl-2, Bax, NF-KB p65 subunit, and IKBC( protein was measured using Western blotting. Results: Gemcitabine 0.01-100 pg/mL inhibited cell proliferation and induced apoptosis in both pancreatic cancer BxPC-3 and COLO 357 cells. Gum mastic 40 pg/mL significantly potentiated the antiproliferative and apoptotic effects of gemcitabine 10 μg/mL after 72-h treatment. When cells were treated with gemcitabine in combination with gum mastic, the IKBα level was increased, whereas NF-κB activation was blocked; the expression of Bax protein was substantially increased, but Bcl-2 protein was down-regulated. Conclusion: Gemcitabine combined with gum mastic causes potent apoptosis in pancreatic cancer cells. The combination may be an effective therapeutic strategy for pancreatic cancer.
identifier
0ISSN: 1671-4083
1EISSN: 1745-7254
2DOI: 10.1038/aps.2010.54
3PMID: 20523344
languageeng
publisherUnited States: Nature Publishing Group
subjectAntimetabolites, Antineoplastic - pharmacology ; Antineoplastic Agents, Phytogenic - pharmacology ; Antineoplastic Agents, Phytogenic - therapeutic use ; apoptosis ; Apoptosis - drug effects ; Bax ; Bax蛋白 ; Bcl-2 ; bcl-2-Associated X Protein - metabolism ; Blotting, Western ; Cell Line, Tumor ; Deoxycytidine - analogs & derivatives ; Deoxycytidine - pharmacology ; Flow Cytometry ; gemcitabine ; Genes, bcl-2 ; Growth Inhibitors - therapeutic use ; gum mastic ; Humans ; I-kappa B Proteins - metabolism ; Mastic Resin ; NF-kappa B - metabolism ; NF-kappaB ; Original ; Original Article ; pancreatic cancer ; Pancreatic Neoplasms - drug therapy ; Pancreatic Neoplasms - pathology ; Resins, Plant - pharmacology ; Resins, Plant - therapeutic use ; Western印迹法 ; 生长抑制 ; 细胞凋亡 ; 细胞增殖 ; 胰腺癌
ispartofActa pharmacologica Sinica, 2010, Vol.31 (6), p.741-745
rights
0Copyright Nature Publishing Group Jun 2010
1Copyright © 2010 CPS and SIMM 2010 CPS and SIMM
lds50peer_reviewed
oafree_for_read
citesFETCH-LOGICAL-1492t-ab0686fc122bbbeeb1cfcf12e7987d83e4634c3c75b74bfe8a4b74fdf8a736ba0
links
openurl$$Topenurl_article
thumbnail$$Uhttp://image.cqvip.com/vip1000/qk/95561A/95561A.jpg
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20523344$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Huang, Xin-yu
1Wang, Hong-cheng
2Yuan, Zhou
3Li, Ang
4He, Mei-lan
5Ai, Kai-xing
6Zheng, Qi
7Qin, Huan-long
title
0Gemcitabine combined with gum mastic causes potent growth inhibition and apoptosis of pancreatic cancer cells
1Acta pharmacologica Sinica
addtitleActa Pharmacologica Sinica
descriptionAim: To investigate the antiproliferative and apoptotic effects of gemcitabine combined with gum mastic and the underlying mechanisms in human pancreatic cancer cell lines. Methods: Cell proliferation and apoptosis were examined using the methyl thiazolyl tetrazolium (MTT) assay and propidium iodine staining, respectively. The expression of Bcl-2, Bax, NF-KB p65 subunit, and IKBC( protein was measured using Western blotting. Results: Gemcitabine 0.01-100 pg/mL inhibited cell proliferation and induced apoptosis in both pancreatic cancer BxPC-3 and COLO 357 cells. Gum mastic 40 pg/mL significantly potentiated the antiproliferative and apoptotic effects of gemcitabine 10 μg/mL after 72-h treatment. When cells were treated with gemcitabine in combination with gum mastic, the IKBα level was increased, whereas NF-κB activation was blocked; the expression of Bax protein was substantially increased, but Bcl-2 protein was down-regulated. Conclusion: Gemcitabine combined with gum mastic causes potent apoptosis in pancreatic cancer cells. The combination may be an effective therapeutic strategy for pancreatic cancer.
subject
0Antimetabolites, Antineoplastic - pharmacology
1Antineoplastic Agents, Phytogenic - pharmacology
2Antineoplastic Agents, Phytogenic - therapeutic use
3apoptosis
4Apoptosis - drug effects
5Bax
6Bax蛋白
7Bcl-2
8bcl-2-Associated X Protein - metabolism
9Blotting, Western
10Cell Line, Tumor
11Deoxycytidine - analogs & derivatives
12Deoxycytidine - pharmacology
13Flow Cytometry
14gemcitabine
15Genes, bcl-2
16Growth Inhibitors - therapeutic use
17gum mastic
18Humans
19I-kappa B Proteins - metabolism
20Mastic Resin
21NF-kappa B - metabolism
22NF-kappaB
23Original
24Original Article
25pancreatic cancer
26Pancreatic Neoplasms - drug therapy
27Pancreatic Neoplasms - pathology
28Resins, Plant - pharmacology
29Resins, Plant - therapeutic use
30Western印迹法
31生长抑制
32细胞凋亡
33细胞增殖
34胰腺癌
issn
01671-4083
11745-7254
fulltextfalse
rsrctypearticle
creationdate2010
recordtypearticle
recordideNpVkc1v1DAQxSMEoqVw4g4WV7TFX4mdCxKqoEWqxAXO1tiZ7Lps7NR2WvHf4yVL1Z481rz5-Y1f07xl9JxRoT_BnM85rbdWPmtOmZLtRvFWPq91p9hGUi1Omlc531AquGD9y-aE05YLIeVpM13i5HwB6wMSF6fDOZB7X3Zku0xkgly8Iw6WjJnMsWAoZJvife37sPPWFx8DgTAQmONcYvaZxJHMEFxCWGeDw0Qc7vf5dfNihH3GN8fzrPn17evPi6vN9Y_L7xdfrjdM9rxswNJOd6NjnFtrES1zoxsZR9VrNWiBshPSCadaq6QdUYOsxTiMGpToLNCz5vPKnRc74eCq6wR7Myc_QfpjInjztBP8zmzjnZGU8l51FXC1AuKMAXzCJ7NDwGLiYHinTC9bLbSzllsnrZAIgGD7uoF0cuh0RX04eknxdsFczE1cUqjrG9EqViNkooo-riKXYs4Jx4f3GDWHlE1N2RxSNq2s6neP13vQ_o-1Ct6vggBleWS-Qg6Mf4ijK7eLYXvrw9ZYcL9Hv0cjJNdt_Uj6F5ixvBo
startdate2010
enddate2010
creator
0Huang, Xin-yu
1Wang, Hong-cheng
2Yuan, Zhou
3Li, Ang
4He, Mei-lan
5Ai, Kai-xing
6Zheng, Qi
7Qin, Huan-long
generalNature Publishing Group
scope
02RA
192L
2CQIGP
3W94
4WU4
5~WA
6CGR
7CUY
8CVF
9ECM
10EIF
11NPM
12AAYXX
13CITATION
143V.
157QP
167QR
177T5
187TK
197TO
207U9
217X7
227XB
2388E
248FD
258FE
268FH
278FI
288FJ
298FK
30ABUWG
31AZQEC
32BBNVY
33BENPR
34BHPHI
35DWQXO
36FR3
37FYUFA
38GHDGH
39GNUQQ
40H94
41HCIFZ
42K9.
43LK8
44M0S
45M1P
46M7N
47M7P
48P64
49PQEST
50PQQKQ
51PQUKI
52PRINS
53BOBZL
54CLFQK
555PM
sort
creationdate2010
titleGemcitabine combined with gum mastic causes potent growth inhibition and apoptosis of pancreatic cancer cells
authorHuang, Xin-yu ; Wang, Hong-cheng ; Yuan, Zhou ; Li, Ang ; He, Mei-lan ; Ai, Kai-xing ; Zheng, Qi ; Qin, Huan-long
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1492t-ab0686fc122bbbeeb1cfcf12e7987d83e4634c3c75b74bfe8a4b74fdf8a736ba0
rsrctypearticles
prefilterarticles
languageeng
creationdate2010
topic
0Antimetabolites, Antineoplastic - pharmacology
1Antineoplastic Agents, Phytogenic - pharmacology
2Antineoplastic Agents, Phytogenic - therapeutic use
3apoptosis
4Apoptosis - drug effects
5Bax
6Bax蛋白
7Bcl-2
8bcl-2-Associated X Protein - metabolism
9Blotting, Western
10Cell Line, Tumor
11Deoxycytidine - analogs & derivatives
12Deoxycytidine - pharmacology
13Flow Cytometry
14gemcitabine
15Genes, bcl-2
16Growth Inhibitors - therapeutic use
17gum mastic
18Humans
19I-kappa B Proteins - metabolism
20Mastic Resin
21NF-kappa B - metabolism
22NF-kappaB
23Original
24Original Article
25pancreatic cancer
26Pancreatic Neoplasms - drug therapy
27Pancreatic Neoplasms - pathology
28Resins, Plant - pharmacology
29Resins, Plant - therapeutic use
30Western印迹法
31生长抑制
32细胞凋亡
33细胞增殖
34胰腺癌
toplevelpeer_reviewed
creatorcontrib
0Huang, Xin-yu
1Wang, Hong-cheng
2Yuan, Zhou
3Li, Ang
4He, Mei-lan
5Ai, Kai-xing
6Zheng, Qi
7Qin, Huan-long
collection
0中文科技期刊数据库
1中文科技期刊数据库-CALIS站点
2中文科技期刊数据库-7.0平台
3中文科技期刊数据库-自然科学
4中文科技期刊数据库-自然科学-生物科学
5中文科技期刊数据库- 镜像站点
6Medline
7MEDLINE
8MEDLINE (Ovid)
9MEDLINE
10MEDLINE
11PubMed
12CrossRef
13ProQuest Central (Corporate)
14Calcium & Calcified Tissue Abstracts
15Chemoreception Abstracts
16Immunology Abstracts
17Neurosciences Abstracts
18Oncogenes and Growth Factors Abstracts
19Virology and AIDS Abstracts
20Health & Medical Collection
21ProQuest Central (purchase pre-March 2016)
22Medical Database (Alumni Edition)
23Technology Research Database
24ProQuest SciTech Collection
25ProQuest Natural Science Collection
26Hospital Premium Collection
27Hospital Premium Collection (Alumni Edition)
28ProQuest Central (Alumni) (purchase pre-March 2016)
29ProQuest Central (Alumni Edition)
30ProQuest Central Essentials
31Biological Science Collection
32ProQuest Central
33Natural Science Collection
34ProQuest Central Korea
35Engineering Research Database
36Health Research Premium Collection
37Health Research Premium Collection (Alumni)
38ProQuest Central Student
39AIDS and Cancer Research Abstracts
40SciTech Premium Collection
41ProQuest Health & Medical Complete (Alumni)
42ProQuest Biological Science Collection
43Health & Medical Collection (Alumni Edition)
44Medical Database
45Algology Mycology and Protozoology Abstracts (Microbiology C)
46Biological Science Database
47Biotechnology and BioEngineering Abstracts
48ProQuest One Academic Eastern Edition
49ProQuest One Academic
50ProQuest One Academic UKI Edition
51ProQuest Central China
52OpenAIRE (Open Access)
53OpenAIRE
54PubMed Central (Full Participant titles)
jtitleActa pharmacologica Sinica
delivery
delcategoryRemote Search Resource
fulltextno_fulltext
addata
au
0Huang, Xin-yu
1Wang, Hong-cheng
2Yuan, Zhou
3Li, Ang
4He, Mei-lan
5Ai, Kai-xing
6Zheng, Qi
7Qin, Huan-long
formatjournal
genrearticle
ristypeJOUR
atitleGemcitabine combined with gum mastic causes potent growth inhibition and apoptosis of pancreatic cancer cells
jtitleActa pharmacologica Sinica
addtitleActa Pharmacologica Sinica
date2010
risdate2010
volume31
issue6
spage741
epage745
pages741-745
issn1671-4083
eissn1745-7254
notes
0Bcl-2
1pancreatic cancer
2Q255
3gum mastic
4Bax
5gemcitabine; gum mastic; pancreatic cancer; apoptosis; NF-kappaB; Bcl-2; Bax
6apoptosis
7gemcitabine
8NF-kappaB
9Q813
1031-1347/R
11These authors contributed equally to the article.
abstractAim: To investigate the antiproliferative and apoptotic effects of gemcitabine combined with gum mastic and the underlying mechanisms in human pancreatic cancer cell lines. Methods: Cell proliferation and apoptosis were examined using the methyl thiazolyl tetrazolium (MTT) assay and propidium iodine staining, respectively. The expression of Bcl-2, Bax, NF-KB p65 subunit, and IKBC( protein was measured using Western blotting. Results: Gemcitabine 0.01-100 pg/mL inhibited cell proliferation and induced apoptosis in both pancreatic cancer BxPC-3 and COLO 357 cells. Gum mastic 40 pg/mL significantly potentiated the antiproliferative and apoptotic effects of gemcitabine 10 μg/mL after 72-h treatment. When cells were treated with gemcitabine in combination with gum mastic, the IKBα level was increased, whereas NF-κB activation was blocked; the expression of Bax protein was substantially increased, but Bcl-2 protein was down-regulated. Conclusion: Gemcitabine combined with gum mastic causes potent apoptosis in pancreatic cancer cells. The combination may be an effective therapeutic strategy for pancreatic cancer.
copUnited States
pubNature Publishing Group
pmid20523344
doi10.1038/aps.2010.54
oafree_for_read