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Shear stress induces osteogenic differentiation of human mesenchymal stem cells

To determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells. hMSCs cultured with or without osteogenic differentiation medium were exposed to fluid flow-induced shear stress and analyzed for alkali... Full description

Journal Title: Regenerative medicine 2010-09-01, Vol.5 (5), p.713-724
Main Author: Yourek, Gregory
Other Authors: McCormick, Susan M , Mao, Jeremy J , Reilly, Gwendolen C
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: England: Future Medicine Ltd
ID: ISSN: 1746-0751
Link: https://www.ncbi.nlm.nih.gov/pubmed/20868327
Zum Text:
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recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4093787
title: Shear stress induces osteogenic differentiation of human mesenchymal stem cells
format: Article
creator:
  • Yourek, Gregory
  • McCormick, Susan M
  • Mao, Jeremy J
  • Reilly, Gwendolen C
subjects:
  • alkaline phosphatase
  • Alkaline Phosphatase - analysis
  • Article
  • Bone Morphogenetic Protein 2 - genetics
  • Bone morphogenetic proteins
  • bone repair
  • Cell Culture Techniques
  • Cell Differentiation
  • Culture Media
  • Humans
  • Influence
  • Kinetics
  • mesenchymal
  • Mesenchymal Stromal Cells - cytology
  • Osteoblasts - cytology
  • Osteogenesis
  • Osteopontin - genetics
  • Properties
  • Rheology
  • RNA, Messenger - analysis
  • Shear (Mechanics)
  • shear stress
  • Stem cells
  • Stress, Mechanical
ispartof: Regenerative medicine, 2010-09-01, Vol.5 (5), p.713-724
description: To determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells. hMSCs cultured with or without osteogenic differentiation medium were exposed to fluid flow-induced shear stress and analyzed for alkaline phosphatase activity and expression of osteogenic genes. Immediately following shear stress, alkaline phosphatase activity in osteogenic medium was significantly increased. At days 4 and 8 of culture the mRNA expression of bone morphogenetic protein-2 and osteopontin was significantly higher in hMSCs subjected to shear stress than those cultured in static conditions. However, hMSCs cultured in osteogenic differentiation medium were less responsive in gene expression of alkaline phosphatase and bone morphogenetic protein-2. These data demonstrate that shear stress stimulates hMSCs towards an osteoblastic phenotype in the absence of chemical induction, suggesting that certain mechanical stresses may serve as an alternative to chemical stimulation of stem cell differentiation.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 1746-0751
fulltext: fulltext
issn:
  • 1746-0751
  • 1746-076X
url: Link


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descriptionTo determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells. hMSCs cultured with or without osteogenic differentiation medium were exposed to fluid flow-induced shear stress and analyzed for alkaline phosphatase activity and expression of osteogenic genes. Immediately following shear stress, alkaline phosphatase activity in osteogenic medium was significantly increased. At days 4 and 8 of culture the mRNA expression of bone morphogenetic protein-2 and osteopontin was significantly higher in hMSCs subjected to shear stress than those cultured in static conditions. However, hMSCs cultured in osteogenic differentiation medium were less responsive in gene expression of alkaline phosphatase and bone morphogenetic protein-2. These data demonstrate that shear stress stimulates hMSCs towards an osteoblastic phenotype in the absence of chemical induction, suggesting that certain mechanical stresses may serve as an alternative to chemical stimulation of stem cell differentiation.
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subjectalkaline phosphatase ; Alkaline Phosphatase - analysis ; Article ; Bone Morphogenetic Protein 2 - genetics ; Bone morphogenetic proteins ; bone repair ; Cell Culture Techniques ; Cell Differentiation ; Culture Media ; Humans ; Influence ; Kinetics ; mesenchymal ; Mesenchymal Stromal Cells - cytology ; Osteoblasts - cytology ; Osteogenesis ; Osteopontin - genetics ; Properties ; Rheology ; RNA, Messenger - analysis ; Shear (Mechanics) ; shear stress ; Stem cells ; Stress, Mechanical
ispartofRegenerative medicine, 2010-09-01, Vol.5 (5), p.713-724
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descriptionTo determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells. hMSCs cultured with or without osteogenic differentiation medium were exposed to fluid flow-induced shear stress and analyzed for alkaline phosphatase activity and expression of osteogenic genes. Immediately following shear stress, alkaline phosphatase activity in osteogenic medium was significantly increased. At days 4 and 8 of culture the mRNA expression of bone morphogenetic protein-2 and osteopontin was significantly higher in hMSCs subjected to shear stress than those cultured in static conditions. However, hMSCs cultured in osteogenic differentiation medium were less responsive in gene expression of alkaline phosphatase and bone morphogenetic protein-2. These data demonstrate that shear stress stimulates hMSCs towards an osteoblastic phenotype in the absence of chemical induction, suggesting that certain mechanical stresses may serve as an alternative to chemical stimulation of stem cell differentiation.
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abstractTo determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells. hMSCs cultured with or without osteogenic differentiation medium were exposed to fluid flow-induced shear stress and analyzed for alkaline phosphatase activity and expression of osteogenic genes. Immediately following shear stress, alkaline phosphatase activity in osteogenic medium was significantly increased. At days 4 and 8 of culture the mRNA expression of bone morphogenetic protein-2 and osteopontin was significantly higher in hMSCs subjected to shear stress than those cultured in static conditions. However, hMSCs cultured in osteogenic differentiation medium were less responsive in gene expression of alkaline phosphatase and bone morphogenetic protein-2. These data demonstrate that shear stress stimulates hMSCs towards an osteoblastic phenotype in the absence of chemical induction, suggesting that certain mechanical stresses may serve as an alternative to chemical stimulation of stem cell differentiation.
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