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Analysis of the sucrose synthase gene family in tobacco: structure, phylogeny, and expression patterns

Main conclusion Provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco and will be beneficial for further investigations of Sus gene functions Sucrose synthase (Sus) has been well characterized as the key enzyme participating in sucrose metabolism, an... Full description

Journal Title: Planta 2015-04-19, Vol.242 (1), p.153-166
Main Author: Wang, Zhong
Other Authors: Wei, Pan , Wu, Mingzhu , Xu, Yalong , Li, Feng , Luo, Zhaopeng , Zhang, Jianfeng , Chen, Ang , Xie, Xiaodong , Cao, Peijian , Lin, Fucheng , Yang, Jun
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Berlin/Heidelberg: Springer Berlin Heidelberg
ID: ISSN: 0032-0935
Link: https://www.ncbi.nlm.nih.gov/pubmed/25893870
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recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4471321
title: Analysis of the sucrose synthase gene family in tobacco: structure, phylogeny, and expression patterns
format: Article
creator:
  • Wang, Zhong
  • Wei, Pan
  • Wu, Mingzhu
  • Xu, Yalong
  • Li, Feng
  • Luo, Zhaopeng
  • Zhang, Jianfeng
  • Chen, Ang
  • Xie, Xiaodong
  • Cao, Peijian
  • Lin, Fucheng
  • Yang, Jun
subjects:
  • Agriculture
  • Amino Acid Motifs - genetics
  • Amino Acid Sequence
  • Biomedical and Life Sciences
  • Cladistic analysis
  • Conserved Sequence
  • Ecology
  • Exons - genetics
  • Forestry
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant
  • Genes
  • Genes, Plant
  • Genetic research
  • Genetics
  • Genomics
  • Glucosyltransferases - chemistry
  • Glucosyltransferases - genetics
  • Glucosyltransferases - metabolism
  • Introns - genetics
  • Isoenzymes
  • Life Sciences
  • Molecular Sequence Data
  • Multigene Family
  • Nicotiana tabacum
  • Open Reading Frames - genetics
  • Original
  • Original Article
  • Phylogeny
  • Physiological aspects
  • Plant Science
  • Plant Sciences
  • Solanaceae
  • Stress
  • Sucrose
  • Tobacco
  • Tobacco - enzymology
  • Tobacco - genetics
ispartof: Planta, 2015-04-19, Vol.242 (1), p.153-166
description: Main conclusion Provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco and will be beneficial for further investigations of Sus gene functions Sucrose synthase (Sus) has been well characterized as the key enzyme participating in sucrose metabolism, and the gene family encoding different Sus isozymes has been cloned and characterized in several plant species. However, scant information about this gene family is available to date in tobacco. Here, we identified 14, 6, and 7 Sus genes in the genomes of Nicotiana tabacum , N. sylvestris and N. tomentosiformis , respectively. These tobacco Sus family members shared high levels of similarity in their nucleotide and amino acid sequences. Phylogenetic analysis revealed distinct evolutionary paths for the tobacco Sus genes. Sus1–4 , Sus5 , and Sus6 – 7 originated from three Sus precursors, respectively, which were generated by duplication before the split of monocots and eudicots. There were two additional duplications, before and after the differentiation of the Solanaceae, which separately gave rise to Sus3/4 and Sus1 / 2 . Gene exon/intron structure analysis showed that the tobacco Sus genes contain varying numbers of conserved introns, resulting from intron loss under different selection pressures during the course of evolution. The expression patterns of the NtSus genes differed from each other in various tobacco tissues. Transcripts of Ntab0259170 and Ntab0259180 were detected in leaves at all tested developmental stages, suggesting that these two genes play a predominant role in sucrose metabolism during leaf development. Expression of Ntab0288750 and Ntab0234340 were conspicuously induced by low temperature and virus treatment, indicating that these two isozymes are important in meeting the increased glycolytic demand that occurs during abiotic stress. Our results provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco, and will be beneficial for further investigations of Sus gene functions in the processes of tobacco leaf development and tobacco resistance to environmental stresses.
language: eng
source:
identifier: ISSN: 0032-0935
fulltext: no_fulltext
issn:
  • 0032-0935
  • 1432-2048
url: Link


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titleAnalysis of the sucrose synthase gene family in tobacco: structure, phylogeny, and expression patterns
creatorWang, Zhong ; Wei, Pan ; Wu, Mingzhu ; Xu, Yalong ; Li, Feng ; Luo, Zhaopeng ; Zhang, Jianfeng ; Chen, Ang ; Xie, Xiaodong ; Cao, Peijian ; Lin, Fucheng ; Yang, Jun
creatorcontribWang, Zhong ; Wei, Pan ; Wu, Mingzhu ; Xu, Yalong ; Li, Feng ; Luo, Zhaopeng ; Zhang, Jianfeng ; Chen, Ang ; Xie, Xiaodong ; Cao, Peijian ; Lin, Fucheng ; Yang, Jun
descriptionMain conclusion Provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco and will be beneficial for further investigations of Sus gene functions Sucrose synthase (Sus) has been well characterized as the key enzyme participating in sucrose metabolism, and the gene family encoding different Sus isozymes has been cloned and characterized in several plant species. However, scant information about this gene family is available to date in tobacco. Here, we identified 14, 6, and 7 Sus genes in the genomes of Nicotiana tabacum , N. sylvestris and N. tomentosiformis , respectively. These tobacco Sus family members shared high levels of similarity in their nucleotide and amino acid sequences. Phylogenetic analysis revealed distinct evolutionary paths for the tobacco Sus genes. Sus1–4 , Sus5 , and Sus6 – 7 originated from three Sus precursors, respectively, which were generated by duplication before the split of monocots and eudicots. There were two additional duplications, before and after the differentiation of the Solanaceae, which separately gave rise to Sus3/4 and Sus1 / 2 . Gene exon/intron structure analysis showed that the tobacco Sus genes contain varying numbers of conserved introns, resulting from intron loss under different selection pressures during the course of evolution. The expression patterns of the NtSus genes differed from each other in various tobacco tissues. Transcripts of Ntab0259170 and Ntab0259180 were detected in leaves at all tested developmental stages, suggesting that these two genes play a predominant role in sucrose metabolism during leaf development. Expression of Ntab0288750 and Ntab0234340 were conspicuously induced by low temperature and virus treatment, indicating that these two isozymes are important in meeting the increased glycolytic demand that occurs during abiotic stress. Our results provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco, and will be beneficial for further investigations of Sus gene functions in the processes of tobacco leaf development and tobacco resistance to environmental stresses.
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languageeng
publisherBerlin/Heidelberg: Springer Berlin Heidelberg
subjectAgriculture ; Amino Acid Motifs - genetics ; Amino Acid Sequence ; Biomedical and Life Sciences ; Cladistic analysis ; Conserved Sequence ; Ecology ; Exons - genetics ; Forestry ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Plant ; Genes ; Genes, Plant ; Genetic research ; Genetics ; Genomics ; Glucosyltransferases - chemistry ; Glucosyltransferases - genetics ; Glucosyltransferases - metabolism ; Introns - genetics ; Isoenzymes ; Life Sciences ; Molecular Sequence Data ; Multigene Family ; Nicotiana tabacum ; Open Reading Frames - genetics ; Original ; Original Article ; Phylogeny ; Physiological aspects ; Plant Science ; Plant Sciences ; Solanaceae ; Stress ; Sucrose ; Tobacco ; Tobacco - enzymology ; Tobacco - genetics
ispartofPlanta, 2015-04-19, Vol.242 (1), p.153-166
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6Zhang, Jianfeng
7Chen, Ang
8Xie, Xiaodong
9Cao, Peijian
10Lin, Fucheng
11Yang, Jun
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descriptionMain conclusion Provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco and will be beneficial for further investigations of Sus gene functions Sucrose synthase (Sus) has been well characterized as the key enzyme participating in sucrose metabolism, and the gene family encoding different Sus isozymes has been cloned and characterized in several plant species. However, scant information about this gene family is available to date in tobacco. Here, we identified 14, 6, and 7 Sus genes in the genomes of Nicotiana tabacum , N. sylvestris and N. tomentosiformis , respectively. These tobacco Sus family members shared high levels of similarity in their nucleotide and amino acid sequences. Phylogenetic analysis revealed distinct evolutionary paths for the tobacco Sus genes. Sus1–4 , Sus5 , and Sus6 – 7 originated from three Sus precursors, respectively, which were generated by duplication before the split of monocots and eudicots. There were two additional duplications, before and after the differentiation of the Solanaceae, which separately gave rise to Sus3/4 and Sus1 / 2 . Gene exon/intron structure analysis showed that the tobacco Sus genes contain varying numbers of conserved introns, resulting from intron loss under different selection pressures during the course of evolution. The expression patterns of the NtSus genes differed from each other in various tobacco tissues. Transcripts of Ntab0259170 and Ntab0259180 were detected in leaves at all tested developmental stages, suggesting that these two genes play a predominant role in sucrose metabolism during leaf development. Expression of Ntab0288750 and Ntab0234340 were conspicuously induced by low temperature and virus treatment, indicating that these two isozymes are important in meeting the increased glycolytic demand that occurs during abiotic stress. Our results provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco, and will be beneficial for further investigations of Sus gene functions in the processes of tobacco leaf development and tobacco resistance to environmental stresses.
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1Amino Acid Motifs - genetics
2Amino Acid Sequence
3Biomedical and Life Sciences
4Cladistic analysis
5Conserved Sequence
6Ecology
7Exons - genetics
8Forestry
9Gene Expression Profiling
10Gene Expression Regulation, Developmental
11Gene Expression Regulation, Enzymologic
12Gene Expression Regulation, Plant
13Genes
14Genes, Plant
15Genetic research
16Genetics
17Genomics
18Glucosyltransferases - chemistry
19Glucosyltransferases - genetics
20Glucosyltransferases - metabolism
21Introns - genetics
22Isoenzymes
23Life Sciences
24Molecular Sequence Data
25Multigene Family
26Nicotiana tabacum
27Open Reading Frames - genetics
28Original
29Original Article
30Phylogeny
31Physiological aspects
32Plant Science
33Plant Sciences
34Solanaceae
35Stress
36Sucrose
37Tobacco
38Tobacco - enzymology
39Tobacco - genetics
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titleAnalysis of the sucrose synthase gene family in tobacco: structure, phylogeny, and expression patterns
authorWang, Zhong ; Wei, Pan ; Wu, Mingzhu ; Xu, Yalong ; Li, Feng ; Luo, Zhaopeng ; Zhang, Jianfeng ; Chen, Ang ; Xie, Xiaodong ; Cao, Peijian ; Lin, Fucheng ; Yang, Jun
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0Agriculture
1Amino Acid Motifs - genetics
2Amino Acid Sequence
3Biomedical and Life Sciences
4Cladistic analysis
5Conserved Sequence
6Ecology
7Exons - genetics
8Forestry
9Gene Expression Profiling
10Gene Expression Regulation, Developmental
11Gene Expression Regulation, Enzymologic
12Gene Expression Regulation, Plant
13Genes
14Genes, Plant
15Genetic research
16Genetics
17Genomics
18Glucosyltransferases - chemistry
19Glucosyltransferases - genetics
20Glucosyltransferases - metabolism
21Introns - genetics
22Isoenzymes
23Life Sciences
24Molecular Sequence Data
25Multigene Family
26Nicotiana tabacum
27Open Reading Frames - genetics
28Original
29Original Article
30Phylogeny
31Physiological aspects
32Plant Science
33Plant Sciences
34Solanaceae
35Stress
36Sucrose
37Tobacco
38Tobacco - enzymology
39Tobacco - genetics
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8Xie, Xiaodong
9Cao, Peijian
10Lin, Fucheng
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abstractMain conclusion Provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco and will be beneficial for further investigations of Sus gene functions Sucrose synthase (Sus) has been well characterized as the key enzyme participating in sucrose metabolism, and the gene family encoding different Sus isozymes has been cloned and characterized in several plant species. However, scant information about this gene family is available to date in tobacco. Here, we identified 14, 6, and 7 Sus genes in the genomes of Nicotiana tabacum , N. sylvestris and N. tomentosiformis , respectively. These tobacco Sus family members shared high levels of similarity in their nucleotide and amino acid sequences. Phylogenetic analysis revealed distinct evolutionary paths for the tobacco Sus genes. Sus1–4 , Sus5 , and Sus6 – 7 originated from three Sus precursors, respectively, which were generated by duplication before the split of monocots and eudicots. There were two additional duplications, before and after the differentiation of the Solanaceae, which separately gave rise to Sus3/4 and Sus1 / 2 . Gene exon/intron structure analysis showed that the tobacco Sus genes contain varying numbers of conserved introns, resulting from intron loss under different selection pressures during the course of evolution. The expression patterns of the NtSus genes differed from each other in various tobacco tissues. Transcripts of Ntab0259170 and Ntab0259180 were detected in leaves at all tested developmental stages, suggesting that these two genes play a predominant role in sucrose metabolism during leaf development. Expression of Ntab0288750 and Ntab0234340 were conspicuously induced by low temperature and virus treatment, indicating that these two isozymes are important in meeting the increased glycolytic demand that occurs during abiotic stress. Our results provide an evolutionary and an empirical molecular genetic foundation of the Sus gene family in tobacco, and will be beneficial for further investigations of Sus gene functions in the processes of tobacco leaf development and tobacco resistance to environmental stresses.
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doi10.1007/s00425-015-2297-1
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