schliessen

Filtern

 

Bibliotheken

Regional Blood Flow in the Normal and Ischemic Brain Is Controlled by Arteriolar Smooth Muscle Cell Contractility and Not by Capillary Pericytes

The precise regulation of cerebral blood flow is critical for normal brain function, and its disruption underlies many neuropathologies. The extent to which smooth muscle-covered arterioles or pericyte-covered capillaries control vasomotion during neurovascular coupling remains controversial. We fou... Full description

Journal Title: Neuron 2015-07-01, Vol.87 (1), p.95-110
Main Author: Hill, Robert A
Other Authors: Tong, Lei , Yuan, Peng , Murikinati, Sasidhar , Gupta, Shobhana , Grutzendler, Jaime
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: United States: Elsevier Inc
ID: ISSN: 0896-6273
Link: https://www.ncbi.nlm.nih.gov/pubmed/26119027
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4487786
title: Regional Blood Flow in the Normal and Ischemic Brain Is Controlled by Arteriolar Smooth Muscle Cell Contractility and Not by Capillary Pericytes
format: Article
creator:
  • Hill, Robert A
  • Tong, Lei
  • Yuan, Peng
  • Murikinati, Sasidhar
  • Gupta, Shobhana
  • Grutzendler, Jaime
subjects:
  • Actins - metabolism
  • Animals
  • Arterioles - physiology
  • Article
  • Blood
  • Brain - blood supply
  • Brain Ischemia
  • Calcium - metabolism
  • Capillaries - physiology
  • cardiovascular system
  • Carotid arteries
  • Cerebral Cortex - blood supply
  • Cerebrovascular Circulation - physiology
  • Experiments
  • Humans
  • Ischemia
  • Mice
  • Morphology
  • Muscle Contraction - physiology
  • Muscle, Smooth, Vascular - cytology
  • Muscle, Smooth, Vascular - physiology
  • Myocytes, Smooth Muscle - physiology
  • Neuropathology
  • Neuroscience(all)
  • Pericytes - physiology
  • Physiology
  • Smooth muscle
  • Studies
  • Veins & arteries
ispartof: Neuron, 2015-07-01, Vol.87 (1), p.95-110
description: The precise regulation of cerebral blood flow is critical for normal brain function, and its disruption underlies many neuropathologies. The extent to which smooth muscle-covered arterioles or pericyte-covered capillaries control vasomotion during neurovascular coupling remains controversial. We found that capillary pericytes in mice and humans do not express smooth muscle actin and are morphologically and functionally distinct from adjacent precapillary smooth muscle cells (SMCs). Using optical imaging we investigated blood flow regulation at various sites on the vascular tree in living mice. Optogenetic, whisker stimulation, or cortical spreading depolarization caused microvascular diameter or flow changes in SMC but not pericyte-covered microvessels. During early stages of brain ischemia, transient SMC but not pericyte constrictions were a major cause of hypoperfusion leading to thrombosis and distal microvascular occlusions. Thus, capillary pericytes are not contractile, and regulation of cerebral blood flow in physiological and pathological conditions is mediated by arteriolar SMCs. •Pericytes are morphologically and functionally distinct from precapillary SMCs•Capillary pericytes lack smooth muscle actin and are not contractile in vivo•Neurovascular coupling is mediated by arteriolar smooth muscle contractility•Ischemia causes transient SMC constrictions that lead to capillary thrombosis The extent to which arteriolar smooth muscle cell or capillary pericyte contractility regulates regional cerebral blood flow is debated. Hill et al. use optogenetic, whisker stimulation, spreading depolarization, and transient cerebral ischemia to demonstrate that capillary pericytes are not contractile in vivo and that arteriolar smooth muscle cells mediate physiological and pathological vasomotion.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0896-6273
fulltext: fulltext
issn:
  • 0896-6273
  • 1097-4199
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.8284268
LOCALfalse
PrimoNMBib
record
control
sourceidgale_pubme
recordidTN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4487786
sourceformatXML
sourcesystemPC
galeidA422581104
sourcerecordidA422581104
originalsourceidFETCH-LOGICAL-1636t-5fe8ec56a206881aebfe36fec6b0b8c68ec38dd1fed745147bec67f8f86bff413
addsrcrecordideNqFUt1qFDEYHUSxtfoGIgFvvNk1mckkGS-E7WK1UKv4cx0yyZdulmyyZjIt-xY-spluLbYXlsAEcn745junql4SPCeYsLfreYAxxTCvMWnnmM0xJo-qQ4I7PqOk6x5Xh1h0bMZq3hxUz4ZhXQi07cjT6qBmhHS45ofV729w4WJQHh37GA068fEKuYDyCtB5TJsCqGDQ6aBXsHEaHSdV0NMBLWPIKXoPBvU7tEgZkoteJfR9E2Neoc_joD2gJXi_5yqdnXd5d-13HvMkW6qt80W0Q1-LXO8yDM-rJ1b5AV7c3EfVz5MPP5afZmdfPp4uF2czwhqWZ60FAbplqsZMCKKgt9AwC5r1uBeaFbARxhALhtOWUN4XiFthBeutpaQ5qt7vfbdjvwGjYRrRy21ymzKPjMrJu0hwK3kRLyWlgnPBisH53iBuISiX4I7WBMjSgBm38srKsnkJllOBBW8xF1hzaIlqWWeYwZY0mIhi-OZmohR_jTBkuXGDLvtTAeI4SMIpE11Lu-5hKusaXnflW6iv71HXcUwl8GtWLXBXeIU137MulAfpgo1TYOWYKfUYwLryvqB13QpCMC0CuhfoFIchgb39e4Ll1E-5lvt-yqmfErNpCUX26t-t34r-FrIQ3t3z1S6r7K4b5PxD7jeZQinOpYMkB-0gaDAlHl3yiO7_Bn8A2_YNWQ
sourcetypeOpen Access Repository
isCDItrue
recordtypearticle
pqid1692809296
display
typearticle
titleRegional Blood Flow in the Normal and Ischemic Brain Is Controlled by Arteriolar Smooth Muscle Cell Contractility and Not by Capillary Pericytes
sourceAlma/SFX Local Collection
creatorHill, Robert A ; Tong, Lei ; Yuan, Peng ; Murikinati, Sasidhar ; Gupta, Shobhana ; Grutzendler, Jaime
creatorcontribHill, Robert A ; Tong, Lei ; Yuan, Peng ; Murikinati, Sasidhar ; Gupta, Shobhana ; Grutzendler, Jaime
descriptionThe precise regulation of cerebral blood flow is critical for normal brain function, and its disruption underlies many neuropathologies. The extent to which smooth muscle-covered arterioles or pericyte-covered capillaries control vasomotion during neurovascular coupling remains controversial. We found that capillary pericytes in mice and humans do not express smooth muscle actin and are morphologically and functionally distinct from adjacent precapillary smooth muscle cells (SMCs). Using optical imaging we investigated blood flow regulation at various sites on the vascular tree in living mice. Optogenetic, whisker stimulation, or cortical spreading depolarization caused microvascular diameter or flow changes in SMC but not pericyte-covered microvessels. During early stages of brain ischemia, transient SMC but not pericyte constrictions were a major cause of hypoperfusion leading to thrombosis and distal microvascular occlusions. Thus, capillary pericytes are not contractile, and regulation of cerebral blood flow in physiological and pathological conditions is mediated by arteriolar SMCs. •Pericytes are morphologically and functionally distinct from precapillary SMCs•Capillary pericytes lack smooth muscle actin and are not contractile in vivo•Neurovascular coupling is mediated by arteriolar smooth muscle contractility•Ischemia causes transient SMC constrictions that lead to capillary thrombosis The extent to which arteriolar smooth muscle cell or capillary pericyte contractility regulates regional cerebral blood flow is debated. Hill et al. use optogenetic, whisker stimulation, spreading depolarization, and transient cerebral ischemia to demonstrate that capillary pericytes are not contractile in vivo and that arteriolar smooth muscle cells mediate physiological and pathological vasomotion.
identifier
0ISSN: 0896-6273
1EISSN: 1097-4199
2DOI: 10.1016/j.neuron.2015.06.001
3PMID: 26119027
languageeng
publisherUnited States: Elsevier Inc
subjectActins - metabolism ; Animals ; Arterioles - physiology ; Article ; Blood ; Brain - blood supply ; Brain Ischemia ; Calcium - metabolism ; Capillaries - physiology ; cardiovascular system ; Carotid arteries ; Cerebral Cortex - blood supply ; Cerebrovascular Circulation - physiology ; Experiments ; Humans ; Ischemia ; Mice ; Morphology ; Muscle Contraction - physiology ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - physiology ; Myocytes, Smooth Muscle - physiology ; Neuropathology ; Neuroscience(all) ; Pericytes - physiology ; Physiology ; Smooth muscle ; Studies ; Veins & arteries
ispartofNeuron, 2015-07-01, Vol.87 (1), p.95-110
rights
02015 Elsevier Inc.
1Copyright © 2015 Elsevier Inc. All rights reserved.
2COPYRIGHT 2015 Elsevier B.V.
3Copyright Elsevier Limited Jul 1, 2015
42015 Published by Elsevier Inc. 2015
lds50peer_reviewed
oafree_for_read
citedbyFETCH-LOGICAL-1636t-5fe8ec56a206881aebfe36fec6b0b8c68ec38dd1fed745147bec67f8f86bff413
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26119027$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Hill, Robert A
1Tong, Lei
2Yuan, Peng
3Murikinati, Sasidhar
4Gupta, Shobhana
5Grutzendler, Jaime
title
0Regional Blood Flow in the Normal and Ischemic Brain Is Controlled by Arteriolar Smooth Muscle Cell Contractility and Not by Capillary Pericytes
1Neuron
addtitleNeuron
descriptionThe precise regulation of cerebral blood flow is critical for normal brain function, and its disruption underlies many neuropathologies. The extent to which smooth muscle-covered arterioles or pericyte-covered capillaries control vasomotion during neurovascular coupling remains controversial. We found that capillary pericytes in mice and humans do not express smooth muscle actin and are morphologically and functionally distinct from adjacent precapillary smooth muscle cells (SMCs). Using optical imaging we investigated blood flow regulation at various sites on the vascular tree in living mice. Optogenetic, whisker stimulation, or cortical spreading depolarization caused microvascular diameter or flow changes in SMC but not pericyte-covered microvessels. During early stages of brain ischemia, transient SMC but not pericyte constrictions were a major cause of hypoperfusion leading to thrombosis and distal microvascular occlusions. Thus, capillary pericytes are not contractile, and regulation of cerebral blood flow in physiological and pathological conditions is mediated by arteriolar SMCs. •Pericytes are morphologically and functionally distinct from precapillary SMCs•Capillary pericytes lack smooth muscle actin and are not contractile in vivo•Neurovascular coupling is mediated by arteriolar smooth muscle contractility•Ischemia causes transient SMC constrictions that lead to capillary thrombosis The extent to which arteriolar smooth muscle cell or capillary pericyte contractility regulates regional cerebral blood flow is debated. Hill et al. use optogenetic, whisker stimulation, spreading depolarization, and transient cerebral ischemia to demonstrate that capillary pericytes are not contractile in vivo and that arteriolar smooth muscle cells mediate physiological and pathological vasomotion.
subject
0Actins - metabolism
1Animals
2Arterioles - physiology
3Article
4Blood
5Brain - blood supply
6Brain Ischemia
7Calcium - metabolism
8Capillaries - physiology
9cardiovascular system
10Carotid arteries
11Cerebral Cortex - blood supply
12Cerebrovascular Circulation - physiology
13Experiments
14Humans
15Ischemia
16Mice
17Morphology
18Muscle Contraction - physiology
19Muscle, Smooth, Vascular - cytology
20Muscle, Smooth, Vascular - physiology
21Myocytes, Smooth Muscle - physiology
22Neuropathology
23Neuroscience(all)
24Pericytes - physiology
25Physiology
26Smooth muscle
27Studies
28Veins & arteries
issn
00896-6273
11097-4199
fulltexttrue
rsrctypearticle
creationdate2015
recordtypearticle
recordideNqFUt1qFDEYHUSxtfoGIgFvvNk1mckkGS-E7WK1UKv4cx0yyZdulmyyZjIt-xY-spluLbYXlsAEcn745junql4SPCeYsLfreYAxxTCvMWnnmM0xJo-qQ4I7PqOk6x5Xh1h0bMZq3hxUz4ZhXQi07cjT6qBmhHS45ofV729w4WJQHh37GA068fEKuYDyCtB5TJsCqGDQ6aBXsHEaHSdV0NMBLWPIKXoPBvU7tEgZkoteJfR9E2Neoc_joD2gJXi_5yqdnXd5d-13HvMkW6qt80W0Q1-LXO8yDM-rJ1b5AV7c3EfVz5MPP5afZmdfPp4uF2czwhqWZ60FAbplqsZMCKKgt9AwC5r1uBeaFbARxhALhtOWUN4XiFthBeutpaQ5qt7vfbdjvwGjYRrRy21ymzKPjMrJu0hwK3kRLyWlgnPBisH53iBuISiX4I7WBMjSgBm38srKsnkJllOBBW8xF1hzaIlqWWeYwZY0mIhi-OZmohR_jTBkuXGDLvtTAeI4SMIpE11Lu-5hKusaXnflW6iv71HXcUwl8GtWLXBXeIU137MulAfpgo1TYOWYKfUYwLryvqB13QpCMC0CuhfoFIchgb39e4Ll1E-5lvt-yqmfErNpCUX26t-t34r-FrIQ3t3z1S6r7K4b5PxD7jeZQinOpYMkB-0gaDAlHl3yiO7_Bn8A2_YNWQ
startdate20150701
enddate20150701
creator
0Hill, Robert A
1Tong, Lei
2Yuan, Peng
3Murikinati, Sasidhar
4Gupta, Shobhana
5Grutzendler, Jaime
general
0Elsevier Inc
1Elsevier B.V
2Elsevier Limited
3Elsevier BV
scope
06I.
1AAFTH
2CGR
3CUY
4CVF
5ECM
6EIF
7NPM
8AAYXX
9CITATION
10BSHEE
117QP
127QR
137TK
148FD
15FR3
16K9.
17P64
18RC3
197X8
20BOBZL
21CLFQK
225PM
sort
creationdate20150701
titleRegional Blood Flow in the Normal and Ischemic Brain Is Controlled by Arteriolar Smooth Muscle Cell Contractility and Not by Capillary Pericytes
authorHill, Robert A ; Tong, Lei ; Yuan, Peng ; Murikinati, Sasidhar ; Gupta, Shobhana ; Grutzendler, Jaime
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1636t-5fe8ec56a206881aebfe36fec6b0b8c68ec38dd1fed745147bec67f8f86bff413
rsrctypearticles
prefilterarticles
languageeng
creationdate2015
topic
0Actins - metabolism
1Animals
2Arterioles - physiology
3Article
4Blood
5Brain - blood supply
6Brain Ischemia
7Calcium - metabolism
8Capillaries - physiology
9cardiovascular system
10Carotid arteries
11Cerebral Cortex - blood supply
12Cerebrovascular Circulation - physiology
13Experiments
14Humans
15Ischemia
16Mice
17Morphology
18Muscle Contraction - physiology
19Muscle, Smooth, Vascular - cytology
20Muscle, Smooth, Vascular - physiology
21Myocytes, Smooth Muscle - physiology
22Neuropathology
23Neuroscience(all)
24Pericytes - physiology
25Physiology
26Smooth muscle
27Studies
28Veins & arteries
toplevel
0peer_reviewed
1online_resources
creatorcontrib
0Hill, Robert A
1Tong, Lei
2Yuan, Peng
3Murikinati, Sasidhar
4Gupta, Shobhana
5Grutzendler, Jaime
collection
0ScienceDirect Open Access Titles
1Elsevier:ScienceDirect:Open Access
2Medline
3MEDLINE
4MEDLINE (Ovid)
5MEDLINE
6MEDLINE
7PubMed
8CrossRef
9Academic OneFile (A&I only)
10Calcium & Calcified Tissue Abstracts
11Chemoreception Abstracts
12Neurosciences Abstracts
13Technology Research Database
14Engineering Research Database
15ProQuest Health & Medical Complete (Alumni)
16Biotechnology and BioEngineering Abstracts
17Genetics Abstracts
18MEDLINE - Academic
19OpenAIRE (Open Access)
20OpenAIRE
21PubMed Central (Full Participant titles)
jtitleNeuron
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Hill, Robert A
1Tong, Lei
2Yuan, Peng
3Murikinati, Sasidhar
4Gupta, Shobhana
5Grutzendler, Jaime
formatjournal
genrearticle
ristypeJOUR
atitleRegional Blood Flow in the Normal and Ischemic Brain Is Controlled by Arteriolar Smooth Muscle Cell Contractility and Not by Capillary Pericytes
jtitleNeuron
addtitleNeuron
date2015-07-01
risdate2015
volume87
issue1
spage95
epage110
pages95-110
issn0896-6273
eissn1097-4199
notesContributed equally to this work
abstractThe precise regulation of cerebral blood flow is critical for normal brain function, and its disruption underlies many neuropathologies. The extent to which smooth muscle-covered arterioles or pericyte-covered capillaries control vasomotion during neurovascular coupling remains controversial. We found that capillary pericytes in mice and humans do not express smooth muscle actin and are morphologically and functionally distinct from adjacent precapillary smooth muscle cells (SMCs). Using optical imaging we investigated blood flow regulation at various sites on the vascular tree in living mice. Optogenetic, whisker stimulation, or cortical spreading depolarization caused microvascular diameter or flow changes in SMC but not pericyte-covered microvessels. During early stages of brain ischemia, transient SMC but not pericyte constrictions were a major cause of hypoperfusion leading to thrombosis and distal microvascular occlusions. Thus, capillary pericytes are not contractile, and regulation of cerebral blood flow in physiological and pathological conditions is mediated by arteriolar SMCs. •Pericytes are morphologically and functionally distinct from precapillary SMCs•Capillary pericytes lack smooth muscle actin and are not contractile in vivo•Neurovascular coupling is mediated by arteriolar smooth muscle contractility•Ischemia causes transient SMC constrictions that lead to capillary thrombosis The extent to which arteriolar smooth muscle cell or capillary pericyte contractility regulates regional cerebral blood flow is debated. Hill et al. use optogenetic, whisker stimulation, spreading depolarization, and transient cerebral ischemia to demonstrate that capillary pericytes are not contractile in vivo and that arteriolar smooth muscle cells mediate physiological and pathological vasomotion.
copUnited States
pubElsevier Inc
pmid26119027
doi10.1016/j.neuron.2015.06.001
oafree_for_read