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IL-25/IL-33–responsive TH2 cells characterize nasal polyps with a default TH17 signature in nasal mucosa

Chronic rhinosinusitis with nasal polyposis (CRSwNP) in Western countries is characterized by eosinophilia, IgE production, and TH2 cytokine expression. Type 2 innate lymphoid cells from polyps produce IL-5 and IL-13 in response to IL-25 and IL-33, although the relevance of this axis to local mucosa... Full description

Journal Title: Journal of allergy and clinical immunology 2016, Vol.137 (5), p.1514-1524
Main Author: Lam, Emily P.S
Other Authors: Kariyawasam, Harsha H , Rana, Batika M.J , Durham, Stephen R , McKenzie, Andrew N.J , Powell, Nicholas , Orban, Nara , Lennartz-Walker, Melissa , Hopkins, Claire , Ying, Sun , Rimmer, Joanne , Lund, Valerie J , Cousins, David J , Till, Stephen J
Format: Electronic Article Electronic Article
Language: English
Subjects:
ST2
Quelle: Alma/SFX Local Collection
Publisher: United States: Elsevier Inc
ID: ISSN: 0091-6749
Link: https://www.ncbi.nlm.nih.gov/pubmed/26684290
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title: IL-25/IL-33–responsive TH2 cells characterize nasal polyps with a default TH17 signature in nasal mucosa
format: Article
creator:
  • Lam, Emily P.S
  • Kariyawasam, Harsha H
  • Rana, Batika M.J
  • Durham, Stephen R
  • McKenzie, Andrew N.J
  • Powell, Nicholas
  • Orban, Nara
  • Lennartz-Walker, Melissa
  • Hopkins, Claire
  • Ying, Sun
  • Rimmer, Joanne
  • Lund, Valerie J
  • Cousins, David J
  • Till, Stephen J
subjects:
  • 1107 Immunology
  • Absent in melanoma 2
  • AIM2, Absent in melanoma 2
  • Allergy
  • CDR3, Complementarity-determining region 3
  • Cell culture
  • cell phenotype
  • cell receptor V beta repertoire
  • cell receptor variable β
  • cell receptor Vβ repertoire
  • chain
  • Chemoattractant receptor
  • Chronic Disease
  • Chronic rhinosinusitis with nasal polyposis
  • Chronic rhinosinusitis with nasal polyps
  • Clinical Immunology
  • Cloning
  • Complementarity
  • CRSwNP
  • CRSwNP, Chronic rhinosinusitis with nasal polyposis
  • CRTH2, Chemoattractant receptor-homologous molecule expressed on TH2 cells
  • Cytokines
  • determining region 3
  • Eosinophilia - immunology
  • Gene expression
  • Genotype & phenotype
  • homologous molecule expressed on TH2 cells
  • Humans
  • IL-17RB
  • IL-25
  • IL-33
  • ILC2, Type 2 innate lymphoid cell
  • Immunology
  • Interleukin-17 - immunology
  • Interleukin-33 - immunology
  • Life Sciences & Biomedicine
  • Lymphocytes
  • Mechanisms of Allergy
  • Mechanisms of Allergy and Clinical Immunology
  • microarray
  • nasal mucosa
  • Nasal Mucosa - immunology
  • Nasal Polyps - immunology
  • Rhinitis - immunology
  • Science & Technology
  • Sinusitis - immunology
  • ST2
  • Studies
  • T cell receptors
  • T(H)17 cells
  • T(H)17 cells
  • T(H)2 cells
  • T-cell phenotype
  • T-cell receptor Vβ repertoire
  • TCR Vβ
  • TCR Vβ, T-cell receptor variable β-chain
  • Th17 Cells - immunology
  • TH17 cells
  • TH2 cells
  • Th2 Cells - immunology
  • Type 2 innate lymphoid cell
ispartof: Journal of allergy and clinical immunology, 2016, Vol.137 (5), p.1514-1524
description: Chronic rhinosinusitis with nasal polyposis (CRSwNP) in Western countries is characterized by eosinophilia, IgE production, and TH2 cytokine expression. Type 2 innate lymphoid cells from polyps produce IL-5 and IL-13 in response to IL-25 and IL-33, although the relevance of this axis to local mucosal T-cell responses is unknown. We sought to investigate the role of the IL-25/IL-33 axis in local mucosal T-cell responses in patients with CRSwNP. Polyp tissue and blood were obtained from patients undergoing nasal polypectomy. Control nasal biopsy specimens and blood were obtained from healthy volunteers. Tissue was cultured in a short-term explant model. T-cell surface phenotype/intracellular cytokines were assessed by means of flow cytometry. T-cell receptor variable β-chain analysis was performed with the immunoSEQ assay. Microarrays were performed for gene expression analysis. IL-25 receptor (IL-17RB)–expressing TH2 effector cells were identified in nasal polyp tissue but not the healthy nasal mucosa or periphery. IL-17RB+CD4+ polyp–derived TH2 cells coexpressed ST2 (IL-33 receptor) and responded to IL-25 and IL-33 with enhanced IL-5 and IL-13 production. Within IL-17RB+CD4+ T cells, several identical T-cell receptor variable β-chain complementarity-determining region 3 sequences were identified in different subjects, suggesting clonal expansion driven by a common antigen. Abundant IL-17–producing T cells were observed in both healthy nasal mucosal and polyp populations, with TH17-related genes the most overexpressed compared with peripheral blood T cells. IL-25 and IL-33 can interact locally with IL-17RB+ST2+ polyp T cells to augment TH2 responses in patients with CRSwNP. A local TH17 response might be important in healthy nasal mucosal immune homeostasis.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0091-6749
fulltext: fulltext
issn:
  • 0091-6749
  • 1097-6825
url: Link


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titleIL-25/IL-33–responsive TH2 cells characterize nasal polyps with a default TH17 signature in nasal mucosa
sourceAlma/SFX Local Collection
creatorLam, Emily P.S ; Kariyawasam, Harsha H ; Rana, Batika M.J ; Durham, Stephen R ; McKenzie, Andrew N.J ; Powell, Nicholas ; Orban, Nara ; Lennartz-Walker, Melissa ; Hopkins, Claire ; Ying, Sun ; Rimmer, Joanne ; Lund, Valerie J ; Cousins, David J ; Till, Stephen J
creatorcontribLam, Emily P.S ; Kariyawasam, Harsha H ; Rana, Batika M.J ; Durham, Stephen R ; McKenzie, Andrew N.J ; Powell, Nicholas ; Orban, Nara ; Lennartz-Walker, Melissa ; Hopkins, Claire ; Ying, Sun ; Rimmer, Joanne ; Lund, Valerie J ; Cousins, David J ; Till, Stephen J
descriptionChronic rhinosinusitis with nasal polyposis (CRSwNP) in Western countries is characterized by eosinophilia, IgE production, and TH2 cytokine expression. Type 2 innate lymphoid cells from polyps produce IL-5 and IL-13 in response to IL-25 and IL-33, although the relevance of this axis to local mucosal T-cell responses is unknown. We sought to investigate the role of the IL-25/IL-33 axis in local mucosal T-cell responses in patients with CRSwNP. Polyp tissue and blood were obtained from patients undergoing nasal polypectomy. Control nasal biopsy specimens and blood were obtained from healthy volunteers. Tissue was cultured in a short-term explant model. T-cell surface phenotype/intracellular cytokines were assessed by means of flow cytometry. T-cell receptor variable β-chain analysis was performed with the immunoSEQ assay. Microarrays were performed for gene expression analysis. IL-25 receptor (IL-17RB)–expressing TH2 effector cells were identified in nasal polyp tissue but not the healthy nasal mucosa or periphery. IL-17RB+CD4+ polyp–derived TH2 cells coexpressed ST2 (IL-33 receptor) and responded to IL-25 and IL-33 with enhanced IL-5 and IL-13 production. Within IL-17RB+CD4+ T cells, several identical T-cell receptor variable β-chain complementarity-determining region 3 sequences were identified in different subjects, suggesting clonal expansion driven by a common antigen. Abundant IL-17–producing T cells were observed in both healthy nasal mucosal and polyp populations, with TH17-related genes the most overexpressed compared with peripheral blood T cells. IL-25 and IL-33 can interact locally with IL-17RB+ST2+ polyp T cells to augment TH2 responses in patients with CRSwNP. A local TH17 response might be important in healthy nasal mucosal immune homeostasis.
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0ISSN: 0091-6749
1EISSN: 1097-6825
2DOI: 10.1016/j.jaci.2015.10.019
3PMID: 26684290
languageeng
publisherUnited States: Elsevier Inc
subject1107 Immunology ; Absent in melanoma 2 ; AIM2, Absent in melanoma 2 ; Allergy ; CDR3, Complementarity-determining region 3 ; Cell culture ; cell phenotype ; cell receptor V beta repertoire ; cell receptor variable β ; cell receptor Vβ repertoire ; chain ; Chemoattractant receptor ; Chronic Disease ; Chronic rhinosinusitis with nasal polyposis ; Chronic rhinosinusitis with nasal polyps ; Clinical Immunology ; Cloning ; Complementarity ; CRSwNP ; CRSwNP, Chronic rhinosinusitis with nasal polyposis ; CRTH2, Chemoattractant receptor-homologous molecule expressed on TH2 cells ; Cytokines ; determining region 3 ; Eosinophilia - immunology ; Gene expression ; Genotype & phenotype ; homologous molecule expressed on TH2 cells ; Humans ; IL-17RB ; IL-25 ; IL-33 ; ILC2, Type 2 innate lymphoid cell ; Immunology ; Interleukin-17 - immunology ; Interleukin-33 - immunology ; Life Sciences & Biomedicine ; Lymphocytes ; Mechanisms of Allergy ; Mechanisms of Allergy and Clinical Immunology ; microarray ; nasal mucosa ; Nasal Mucosa - immunology ; Nasal Polyps - immunology ; Rhinitis - immunology ; Science & Technology ; Sinusitis - immunology ; ST2 ; Studies ; T cell receptors ; T(H)17 cells ; T(H)17 cells ; T(H)2 cells ; T-cell phenotype ; T-cell receptor Vβ repertoire ; TCR Vβ ; TCR Vβ, T-cell receptor variable β-chain ; Th17 Cells - immunology ; TH17 cells ; TH2 cells ; Th2 Cells - immunology ; Type 2 innate lymphoid cell
ispartofJournal of allergy and clinical immunology, 2016, Vol.137 (5), p.1514-1524
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02015 The Authors
1Copyright © 2015 The Authors. Published by Elsevier Inc. All rights reserved.
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32015 The Authors 2015
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0Lam, Emily P.S
1Kariyawasam, Harsha H
2Rana, Batika M.J
3Durham, Stephen R
4McKenzie, Andrew N.J
5Powell, Nicholas
6Orban, Nara
7Lennartz-Walker, Melissa
8Hopkins, Claire
9Ying, Sun
10Rimmer, Joanne
11Lund, Valerie J
12Cousins, David J
13Till, Stephen J
title
0IL-25/IL-33–responsive TH2 cells characterize nasal polyps with a default TH17 signature in nasal mucosa
1Journal of allergy and clinical immunology
addtitleJ Allergy Clin Immunol
descriptionChronic rhinosinusitis with nasal polyposis (CRSwNP) in Western countries is characterized by eosinophilia, IgE production, and TH2 cytokine expression. Type 2 innate lymphoid cells from polyps produce IL-5 and IL-13 in response to IL-25 and IL-33, although the relevance of this axis to local mucosal T-cell responses is unknown. We sought to investigate the role of the IL-25/IL-33 axis in local mucosal T-cell responses in patients with CRSwNP. Polyp tissue and blood were obtained from patients undergoing nasal polypectomy. Control nasal biopsy specimens and blood were obtained from healthy volunteers. Tissue was cultured in a short-term explant model. T-cell surface phenotype/intracellular cytokines were assessed by means of flow cytometry. T-cell receptor variable β-chain analysis was performed with the immunoSEQ assay. Microarrays were performed for gene expression analysis. IL-25 receptor (IL-17RB)–expressing TH2 effector cells were identified in nasal polyp tissue but not the healthy nasal mucosa or periphery. IL-17RB+CD4+ polyp–derived TH2 cells coexpressed ST2 (IL-33 receptor) and responded to IL-25 and IL-33 with enhanced IL-5 and IL-13 production. Within IL-17RB+CD4+ T cells, several identical T-cell receptor variable β-chain complementarity-determining region 3 sequences were identified in different subjects, suggesting clonal expansion driven by a common antigen. Abundant IL-17–producing T cells were observed in both healthy nasal mucosal and polyp populations, with TH17-related genes the most overexpressed compared with peripheral blood T cells. IL-25 and IL-33 can interact locally with IL-17RB+ST2+ polyp T cells to augment TH2 responses in patients with CRSwNP. A local TH17 response might be important in healthy nasal mucosal immune homeostasis.
subject
01107 Immunology
1Absent in melanoma 2
2AIM2, Absent in melanoma 2
3Allergy
4CDR3, Complementarity-determining region 3
5Cell culture
6cell phenotype
7cell receptor V beta repertoire
8cell receptor variable β
9cell receptor Vβ repertoire
10chain
11Chemoattractant receptor
12Chronic Disease
13Chronic rhinosinusitis with nasal polyposis
14Chronic rhinosinusitis with nasal polyps
15Clinical Immunology
16Cloning
17Complementarity
18CRSwNP
19CRSwNP, Chronic rhinosinusitis with nasal polyposis
20CRTH2, Chemoattractant receptor-homologous molecule expressed on TH2 cells
21Cytokines
22determining region 3
23Eosinophilia - immunology
24Gene expression
25Genotype & phenotype
26homologous molecule expressed on TH2 cells
27Humans
28IL-17RB
29IL-25
30IL-33
31ILC2, Type 2 innate lymphoid cell
32Immunology
33Interleukin-17 - immunology
34Interleukin-33 - immunology
35Life Sciences & Biomedicine
36Lymphocytes
37Mechanisms of Allergy
38Mechanisms of Allergy and Clinical Immunology
39microarray
40nasal mucosa
41Nasal Mucosa - immunology
42Nasal Polyps - immunology
43Rhinitis - immunology
44Science & Technology
45Sinusitis - immunology
46ST2
47Studies
48T cell receptors
49T(H)17 cells
50T(H)17 cells
51T(H)2 cells
52T-cell phenotype
53T-cell receptor Vβ repertoire
54TCR Vβ
55TCR Vβ, T-cell receptor variable β-chain
56Th17 Cells - immunology
57TH17 cells
58TH2 cells
59Th2 Cells - immunology
60Type 2 innate lymphoid cell
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0Lam, Emily P.S
1Kariyawasam, Harsha H
2Rana, Batika M.J
3Durham, Stephen R
4McKenzie, Andrew N.J
5Powell, Nicholas
6Orban, Nara
7Lennartz-Walker, Melissa
8Hopkins, Claire
9Ying, Sun
10Rimmer, Joanne
11Lund, Valerie J
12Cousins, David J
13Till, Stephen J
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titleIL-25/IL-33–responsive TH2 cells characterize nasal polyps with a default TH17 signature in nasal mucosa
authorLam, Emily P.S ; Kariyawasam, Harsha H ; Rana, Batika M.J ; Durham, Stephen R ; McKenzie, Andrew N.J ; Powell, Nicholas ; Orban, Nara ; Lennartz-Walker, Melissa ; Hopkins, Claire ; Ying, Sun ; Rimmer, Joanne ; Lund, Valerie J ; Cousins, David J ; Till, Stephen J
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01107 Immunology
1Absent in melanoma 2
2AIM2, Absent in melanoma 2
3Allergy
4CDR3, Complementarity-determining region 3
5Cell culture
6cell phenotype
7cell receptor V beta repertoire
8cell receptor variable β
9cell receptor Vβ repertoire
10chain
11Chemoattractant receptor
12Chronic Disease
13Chronic rhinosinusitis with nasal polyposis
14Chronic rhinosinusitis with nasal polyps
15Clinical Immunology
16Cloning
17Complementarity
18CRSwNP
19CRSwNP, Chronic rhinosinusitis with nasal polyposis
20CRTH2, Chemoattractant receptor-homologous molecule expressed on TH2 cells
21Cytokines
22determining region 3
23Eosinophilia - immunology
24Gene expression
25Genotype & phenotype
26homologous molecule expressed on TH2 cells
27Humans
28IL-17RB
29IL-25
30IL-33
31ILC2, Type 2 innate lymphoid cell
32Immunology
33Interleukin-17 - immunology
34Interleukin-33 - immunology
35Life Sciences & Biomedicine
36Lymphocytes
37Mechanisms of Allergy
38Mechanisms of Allergy and Clinical Immunology
39microarray
40nasal mucosa
41Nasal Mucosa - immunology
42Nasal Polyps - immunology
43Rhinitis - immunology
44Science & Technology
45Sinusitis - immunology
46ST2
47Studies
48T cell receptors
49T(H)17 cells
50T(H)17 cells
51T(H)2 cells
52T-cell phenotype
53T-cell receptor Vβ repertoire
54TCR Vβ
55TCR Vβ, T-cell receptor variable β-chain
56Th17 Cells - immunology
57TH17 cells
58TH2 cells
59Th2 Cells - immunology
60Type 2 innate lymphoid cell
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1Kariyawasam, Harsha H
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7Lennartz-Walker, Melissa
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6Orban, Nara
7Lennartz-Walker, Melissa
8Hopkins, Claire
9Ying, Sun
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abstractChronic rhinosinusitis with nasal polyposis (CRSwNP) in Western countries is characterized by eosinophilia, IgE production, and TH2 cytokine expression. Type 2 innate lymphoid cells from polyps produce IL-5 and IL-13 in response to IL-25 and IL-33, although the relevance of this axis to local mucosal T-cell responses is unknown. We sought to investigate the role of the IL-25/IL-33 axis in local mucosal T-cell responses in patients with CRSwNP. Polyp tissue and blood were obtained from patients undergoing nasal polypectomy. Control nasal biopsy specimens and blood were obtained from healthy volunteers. Tissue was cultured in a short-term explant model. T-cell surface phenotype/intracellular cytokines were assessed by means of flow cytometry. T-cell receptor variable β-chain analysis was performed with the immunoSEQ assay. Microarrays were performed for gene expression analysis. IL-25 receptor (IL-17RB)–expressing TH2 effector cells were identified in nasal polyp tissue but not the healthy nasal mucosa or periphery. IL-17RB+CD4+ polyp–derived TH2 cells coexpressed ST2 (IL-33 receptor) and responded to IL-25 and IL-33 with enhanced IL-5 and IL-13 production. Within IL-17RB+CD4+ T cells, several identical T-cell receptor variable β-chain complementarity-determining region 3 sequences were identified in different subjects, suggesting clonal expansion driven by a common antigen. Abundant IL-17–producing T cells were observed in both healthy nasal mucosal and polyp populations, with TH17-related genes the most overexpressed compared with peripheral blood T cells. IL-25 and IL-33 can interact locally with IL-17RB+ST2+ polyp T cells to augment TH2 responses in patients with CRSwNP. A local TH17 response might be important in healthy nasal mucosal immune homeostasis.
copUnited States
pubElsevier Inc
pmid26684290
doi10.1016/j.jaci.2015.10.019
orcididhttps://orcid.org/0000-0002-2169-9231
oafree_for_read