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Detection of circulating tumor cells from cryopreserved human sarcoma peripheral blood mononuclear cells

Circulating tumor cells (CTCs) enter the vasculature or lymphatic system after shedding from the primary tumor. CTCs may serve as “seed” cells for tumor metastasis. The utility of CTCs in clinical applications for sarcoma is not fully investigated, partly owing to the necessity for fresh blood sampl... Full description

Journal Title: Cancer letters 2017-09-10, Vol.403, p.216-223
Main Author: Li, Heming
Other Authors: Meng, Qing H , Noh, Hyangsoon , Batth, Izhar Singh , Somaiah, Neeta , Torres, Keila E , Xia, Xueqing , Wang, Ruoyu , Li, Shulin
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Ireland: Elsevier B.V
ID: ISSN: 0304-3835
Link: https://www.ncbi.nlm.nih.gov/pubmed/28652021
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title: Detection of circulating tumor cells from cryopreserved human sarcoma peripheral blood mononuclear cells
format: Article
creator:
  • Li, Heming
  • Meng, Qing H
  • Noh, Hyangsoon
  • Batth, Izhar Singh
  • Somaiah, Neeta
  • Torres, Keila E
  • Xia, Xueqing
  • Wang, Ruoyu
  • Li, Shulin
subjects:
  • Analysis
  • Antibodies
  • Article
  • Biomarkers, Tumor - blood
  • Blood
  • Blood circulation
  • Bone Neoplasms - blood
  • Bone Neoplasms - pathology
  • Breast cancer
  • Cancer
  • Cell Line, Tumor
  • Cell surface
  • Cell surface vimentin
  • Cells
  • Circulating tumor cells
  • Cryopreservation
  • Cryotherapy
  • Disease
  • Feasibility studies
  • Fluorescent Antibody Technique
  • Humans
  • Immunomagnetic Separation - methods
  • Laboratories
  • Leukocytes (mononuclear)
  • Leukocytes, Mononuclear - chemistry
  • Leukocytes, Mononuclear - pathology
  • Lymphatic system
  • Markers
  • Metastases
  • Metastasis
  • Methods
  • Neoplastic Cells, Circulating - chemistry
  • Neoplastic Cells, Circulating - pathology
  • Nuclear medicine
  • Osteosarcoma - blood
  • Osteosarcoma - pathology
  • Peripheral blood mononuclear cells
  • Predictive Value of Tests
  • Prognosis
  • Reproducibility
  • Reproducibility of Results
  • Sarcoma
  • Shedding
  • Therapeutic applications
  • Time Factors
  • Tumor cells
  • Tumor markers
  • Tumors
  • Usage
  • Vimentin
  • Vimentin - blood
  • Viral antibodies
ispartof: Cancer letters, 2017-09-10, Vol.403, p.216-223
description: Circulating tumor cells (CTCs) enter the vasculature or lymphatic system after shedding from the primary tumor. CTCs may serve as “seed” cells for tumor metastasis. The utility of CTCs in clinical applications for sarcoma is not fully investigated, partly owing to the necessity for fresh blood samples and the lack of a CTC-specific antibody. To overcome these drawbacks, we developed a technique for sarcoma CTCs capture and detection using cryopreserved peripheral blood mononuclear cells (PBMCs) and our proprietary cell-surface vimentin (CSV) antibody 84-1, which is specific to tumor cells. This technique was validated by sarcoma cell spiking assay, matched CTCs comparison between fresh and cryopreserved PBMCs, and independent tumor markers in multiple types of sarcoma patient blood samples. The reproducibility was maximized when cryopreserved PBMCs were prepared from fresh blood samples within 2 h of the blood draw. In summary, as far as we are aware, ours is the first report to capture and detect CTCs from cryopreserved PBMCs. Further validation in other types of tumor may help boost the feasibility and utility of CTC-based diagnosis in a centralized laboratory. •Cell-surface vimentin (CSV) is a specific marker to capture CTCs from patient cryopreserved PBMCs across sarcoma tumor types.•The novel technology is validated to specifically detect and isolate sarcoma CTCs from cryopreserved PBMCs.•This technique allows for the wide use of CTC-based diagnosis and treatment in clinical settings for sarcoma patients.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0304-3835
fulltext: fulltext
issn:
  • 0304-3835
  • 1872-7980
url: Link


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titleDetection of circulating tumor cells from cryopreserved human sarcoma peripheral blood mononuclear cells
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creatorLi, Heming ; Meng, Qing H ; Noh, Hyangsoon ; Batth, Izhar Singh ; Somaiah, Neeta ; Torres, Keila E ; Xia, Xueqing ; Wang, Ruoyu ; Li, Shulin
creatorcontribLi, Heming ; Meng, Qing H ; Noh, Hyangsoon ; Batth, Izhar Singh ; Somaiah, Neeta ; Torres, Keila E ; Xia, Xueqing ; Wang, Ruoyu ; Li, Shulin
descriptionCirculating tumor cells (CTCs) enter the vasculature or lymphatic system after shedding from the primary tumor. CTCs may serve as “seed” cells for tumor metastasis. The utility of CTCs in clinical applications for sarcoma is not fully investigated, partly owing to the necessity for fresh blood samples and the lack of a CTC-specific antibody. To overcome these drawbacks, we developed a technique for sarcoma CTCs capture and detection using cryopreserved peripheral blood mononuclear cells (PBMCs) and our proprietary cell-surface vimentin (CSV) antibody 84-1, which is specific to tumor cells. This technique was validated by sarcoma cell spiking assay, matched CTCs comparison between fresh and cryopreserved PBMCs, and independent tumor markers in multiple types of sarcoma patient blood samples. The reproducibility was maximized when cryopreserved PBMCs were prepared from fresh blood samples within 2 h of the blood draw. In summary, as far as we are aware, ours is the first report to capture and detect CTCs from cryopreserved PBMCs. Further validation in other types of tumor may help boost the feasibility and utility of CTC-based diagnosis in a centralized laboratory. •Cell-surface vimentin (CSV) is a specific marker to capture CTCs from patient cryopreserved PBMCs across sarcoma tumor types.•The novel technology is validated to specifically detect and isolate sarcoma CTCs from cryopreserved PBMCs.•This technique allows for the wide use of CTC-based diagnosis and treatment in clinical settings for sarcoma patients.
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languageeng
publisherIreland: Elsevier B.V
subjectAnalysis ; Antibodies ; Article ; Biomarkers, Tumor - blood ; Blood ; Blood circulation ; Bone Neoplasms - blood ; Bone Neoplasms - pathology ; Breast cancer ; Cancer ; Cell Line, Tumor ; Cell surface ; Cell surface vimentin ; Cells ; Circulating tumor cells ; Cryopreservation ; Cryotherapy ; Disease ; Feasibility studies ; Fluorescent Antibody Technique ; Humans ; Immunomagnetic Separation - methods ; Laboratories ; Leukocytes (mononuclear) ; Leukocytes, Mononuclear - chemistry ; Leukocytes, Mononuclear - pathology ; Lymphatic system ; Markers ; Metastases ; Metastasis ; Methods ; Neoplastic Cells, Circulating - chemistry ; Neoplastic Cells, Circulating - pathology ; Nuclear medicine ; Osteosarcoma - blood ; Osteosarcoma - pathology ; Peripheral blood mononuclear cells ; Predictive Value of Tests ; Prognosis ; Reproducibility ; Reproducibility of Results ; Sarcoma ; Shedding ; Therapeutic applications ; Time Factors ; Tumor cells ; Tumor markers ; Tumors ; Usage ; Vimentin ; Vimentin - blood ; Viral antibodies
ispartofCancer letters, 2017-09-10, Vol.403, p.216-223
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5Torres, Keila E
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7Wang, Ruoyu
8Li, Shulin
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0Detection of circulating tumor cells from cryopreserved human sarcoma peripheral blood mononuclear cells
1Cancer letters
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descriptionCirculating tumor cells (CTCs) enter the vasculature or lymphatic system after shedding from the primary tumor. CTCs may serve as “seed” cells for tumor metastasis. The utility of CTCs in clinical applications for sarcoma is not fully investigated, partly owing to the necessity for fresh blood samples and the lack of a CTC-specific antibody. To overcome these drawbacks, we developed a technique for sarcoma CTCs capture and detection using cryopreserved peripheral blood mononuclear cells (PBMCs) and our proprietary cell-surface vimentin (CSV) antibody 84-1, which is specific to tumor cells. This technique was validated by sarcoma cell spiking assay, matched CTCs comparison between fresh and cryopreserved PBMCs, and independent tumor markers in multiple types of sarcoma patient blood samples. The reproducibility was maximized when cryopreserved PBMCs were prepared from fresh blood samples within 2 h of the blood draw. In summary, as far as we are aware, ours is the first report to capture and detect CTCs from cryopreserved PBMCs. Further validation in other types of tumor may help boost the feasibility and utility of CTC-based diagnosis in a centralized laboratory. •Cell-surface vimentin (CSV) is a specific marker to capture CTCs from patient cryopreserved PBMCs across sarcoma tumor types.•The novel technology is validated to specifically detect and isolate sarcoma CTCs from cryopreserved PBMCs.•This technique allows for the wide use of CTC-based diagnosis and treatment in clinical settings for sarcoma patients.
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1Antibodies
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3Biomarkers, Tumor - blood
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5Blood circulation
6Bone Neoplasms - blood
7Bone Neoplasms - pathology
8Breast cancer
9Cancer
10Cell Line, Tumor
11Cell surface
12Cell surface vimentin
13Cells
14Circulating tumor cells
15Cryopreservation
16Cryotherapy
17Disease
18Feasibility studies
19Fluorescent Antibody Technique
20Humans
21Immunomagnetic Separation - methods
22Laboratories
23Leukocytes (mononuclear)
24Leukocytes, Mononuclear - chemistry
25Leukocytes, Mononuclear - pathology
26Lymphatic system
27Markers
28Metastases
29Metastasis
30Methods
31Neoplastic Cells, Circulating - chemistry
32Neoplastic Cells, Circulating - pathology
33Nuclear medicine
34Osteosarcoma - blood
35Osteosarcoma - pathology
36Peripheral blood mononuclear cells
37Predictive Value of Tests
38Prognosis
39Reproducibility
40Reproducibility of Results
41Sarcoma
42Shedding
43Therapeutic applications
44Time Factors
45Tumor cells
46Tumor markers
47Tumors
48Usage
49Vimentin
50Vimentin - blood
51Viral antibodies
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titleDetection of circulating tumor cells from cryopreserved human sarcoma peripheral blood mononuclear cells
authorLi, Heming ; Meng, Qing H ; Noh, Hyangsoon ; Batth, Izhar Singh ; Somaiah, Neeta ; Torres, Keila E ; Xia, Xueqing ; Wang, Ruoyu ; Li, Shulin
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37Predictive Value of Tests
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42Shedding
43Therapeutic applications
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46Tumor markers
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50Vimentin - blood
51Viral antibodies
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7Wang, Ruoyu
8Li, Shulin
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atitleDetection of circulating tumor cells from cryopreserved human sarcoma peripheral blood mononuclear cells
jtitleCancer letters
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date2017-09-10
risdate2017
volume403
spage216
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pages216-223
issn0304-3835
eissn1872-7980
abstractCirculating tumor cells (CTCs) enter the vasculature or lymphatic system after shedding from the primary tumor. CTCs may serve as “seed” cells for tumor metastasis. The utility of CTCs in clinical applications for sarcoma is not fully investigated, partly owing to the necessity for fresh blood samples and the lack of a CTC-specific antibody. To overcome these drawbacks, we developed a technique for sarcoma CTCs capture and detection using cryopreserved peripheral blood mononuclear cells (PBMCs) and our proprietary cell-surface vimentin (CSV) antibody 84-1, which is specific to tumor cells. This technique was validated by sarcoma cell spiking assay, matched CTCs comparison between fresh and cryopreserved PBMCs, and independent tumor markers in multiple types of sarcoma patient blood samples. The reproducibility was maximized when cryopreserved PBMCs were prepared from fresh blood samples within 2 h of the blood draw. In summary, as far as we are aware, ours is the first report to capture and detect CTCs from cryopreserved PBMCs. Further validation in other types of tumor may help boost the feasibility and utility of CTC-based diagnosis in a centralized laboratory. •Cell-surface vimentin (CSV) is a specific marker to capture CTCs from patient cryopreserved PBMCs across sarcoma tumor types.•The novel technology is validated to specifically detect and isolate sarcoma CTCs from cryopreserved PBMCs.•This technique allows for the wide use of CTC-based diagnosis and treatment in clinical settings for sarcoma patients.
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