schliessen

Filtern

 

Bibliotheken

The Spleen Is an HIV-1 Sanctuary During Combined Antiretroviral Therapy

Combined antiretroviral therapy (cART) does not eradicate HIV, which persists for years and can re-establish replication if treatment is stopped. The current challenge is identifying those tissues harboring virus through cART. Here, we used HIV env-nef single genome sequencing and HIV gag droplet di... Full description

Journal Title: AIDS research and human retroviruses 2018, Vol.34 (1), p.123-125
Main Author: Nolan, David J.
Other Authors: Rose, Rebecca , Rodriguez, Patricia H. , Salemi, Marco , Singer, Elyse J. , Lamers, Susanna L. , McGrath, Michael S.
Format: Electronic Article Electronic Article
Language: English
Subjects:
HIV
Quelle: Alma/SFX Local Collection
Publisher: United States: Mary Ann Liebert, Inc
ID: ISSN: 0889-2229
Link: https://www.ncbi.nlm.nih.gov/pubmed/29084441
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5771542
title: The Spleen Is an HIV-1 Sanctuary During Combined Antiretroviral Therapy
format: Article
creator:
  • Nolan, David J.
  • Rose, Rebecca
  • Rodriguez, Patricia H.
  • Salemi, Marco
  • Singer, Elyse J.
  • Lamers, Susanna L.
  • McGrath, Michael S.
subjects:
  • Adult
  • Aged
  • Anti-Retroviral Agents - therapeutic use
  • Antiretroviral agents
  • Antiretroviral drugs
  • Antiretroviral therapy
  • Antiretroviral Therapy, Highly Active
  • Drug therapy
  • Gag protein
  • Gene sequencing
  • Genes, gag
  • Genomes
  • HIV
  • HIV evolution
  • HIV Infections - mortality
  • HIV Infections - virology
  • HIV Seropositivity
  • HIV-1 - drug effects
  • HIV-1 - genetics
  • Human immunodeficiency virus
  • Humans
  • lymphatic system
  • Male
  • Middle Aged
  • phylogenetics
  • Phylogeny
  • Polymerase Chain Reaction
  • Proviruses - genetics
  • RNA, Viral - blood
  • Sequence Note
  • Spleen
  • Spleen - virology
  • Therapy
  • Tissues
  • Viral Load
  • Viral Proteins - genetics
  • viral reservoirs
  • virus diseases
  • Viruses
ispartof: AIDS research and human retroviruses, 2018, Vol.34 (1), p.123-125
description: Combined antiretroviral therapy (cART) does not eradicate HIV, which persists for years and can re-establish replication if treatment is stopped. The current challenge is identifying those tissues harboring virus through cART. Here, we used HIV env-nef single genome sequencing and HIV gag droplet digital PCR (ddPCR) to survey 50 tissues from five subjects on cART with no detectable plasma viral load at death. The spleen most consistently contained multiple proviral and expressed sequences (4/5 participants). Spleen-derived HIV demonstrated two distinct phylogenetic patterns: multiple identical sequences, often from different tissues, as well as diverse viral sequences on long terminal branches. Our results suggested that ddPCR may overestimate the size of the tissue-based viral reservoir. The spleen, a lymphatic organ at the intersection of the immune and circulatory systems, may play a key role in viral persistence.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0889-2229
fulltext: fulltext
issn:
  • 0889-2229
  • 1931-8405
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.5856793
LOCALfalse
PrimoNMBib
record
control
sourceidproquest_pubme
recordidTN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5771542
sourceformatXML
sourcesystemPC
sourcerecordid1958540251
originalsourceidFETCH-LOGICAL-1577t-f0a6d1376a67cbe17c09aaf8c00270a7a0bcd571344521c756366954d0ec01fe3
addsrcrecordideNqNkcFvFCEUxonR2LV69Gom8eJl1gcDA1xMNlvtbtLEQ6tXwgDT0szCyjBN-t_LZGvTNjV64vB-38f33ofQewxLDEJ-1t4uCWC-BMLoC7TAssG1oMBeogUIIWtCiDxCb8bxGgAkIew1OiISBKUUL9DpxZWrzveDc6HajpUO1Wb7s8bVuQ4mTzrdVidT8uGyWsdd54Oz1Spkn1xO8cYnPVRFn_T-9i161ethdO_u3mP049vXi_WmPvt-ul2vzmrMOM91D7q1uOGtbrnpHOYGpNa9MACEg-YaOmMZxw2ljGDDWdu0rWTUgjOAe9ccoy8H3_3U7Zw1LuSSQu2T35WwKmqvHk-Cv1KX8UaV7zGjpBhsDgZx74IumzzS2uCyilaRlishWO86aXljetn3urOiYbJj2FiwAtNi9ekuS4q_JjdmtfOjccOgg4vTqLBkgtHSCy7oxyfodZxSKJcqlJjNQDSFqg-USXEck-vvs2FQc9uqtK3mttXcduE_PDzGPf2n3gI0TwyNzzr7ON_GD3-1_T_VanvynGqOoEMYvOtcyv9Y4TfpONZn
sourcetypeOpen Access Repository
isCDItrue
recordtypearticle
pqid1988143083
display
typearticle
titleThe Spleen Is an HIV-1 Sanctuary During Combined Antiretroviral Therapy
sourceAlma/SFX Local Collection
creatorNolan, David J. ; Rose, Rebecca ; Rodriguez, Patricia H. ; Salemi, Marco ; Singer, Elyse J. ; Lamers, Susanna L. ; McGrath, Michael S.
creatorcontribNolan, David J. ; Rose, Rebecca ; Rodriguez, Patricia H. ; Salemi, Marco ; Singer, Elyse J. ; Lamers, Susanna L. ; McGrath, Michael S.
descriptionCombined antiretroviral therapy (cART) does not eradicate HIV, which persists for years and can re-establish replication if treatment is stopped. The current challenge is identifying those tissues harboring virus through cART. Here, we used HIV env-nef single genome sequencing and HIV gag droplet digital PCR (ddPCR) to survey 50 tissues from five subjects on cART with no detectable plasma viral load at death. The spleen most consistently contained multiple proviral and expressed sequences (4/5 participants). Spleen-derived HIV demonstrated two distinct phylogenetic patterns: multiple identical sequences, often from different tissues, as well as diverse viral sequences on long terminal branches. Our results suggested that ddPCR may overestimate the size of the tissue-based viral reservoir. The spleen, a lymphatic organ at the intersection of the immune and circulatory systems, may play a key role in viral persistence.
identifier
0ISSN: 0889-2229
1EISSN: 1931-8405
2DOI: 10.1089/aid.2017.0254
3PMID: 29084441
languageeng
publisherUnited States: Mary Ann Liebert, Inc
subjectAdult ; Aged ; Anti-Retroviral Agents - therapeutic use ; Antiretroviral agents ; Antiretroviral drugs ; Antiretroviral therapy ; Antiretroviral Therapy, Highly Active ; Drug therapy ; Gag protein ; Gene sequencing ; Genes, gag ; Genomes ; HIV ; HIV evolution ; HIV Infections - mortality ; HIV Infections - virology ; HIV Seropositivity ; HIV-1 - drug effects ; HIV-1 - genetics ; Human immunodeficiency virus ; Humans ; lymphatic system ; Male ; Middle Aged ; phylogenetics ; Phylogeny ; Polymerase Chain Reaction ; Proviruses - genetics ; RNA, Viral - blood ; Sequence Note ; Spleen ; Spleen - virology ; Therapy ; Tissues ; Viral Load ; Viral Proteins - genetics ; viral reservoirs ; virus diseases ; Viruses
ispartofAIDS research and human retroviruses, 2018, Vol.34 (1), p.123-125
rights
02018, Mary Ann Liebert, Inc.
1(©) Copyright 2018, Mary Ann Liebert, Inc.
2Copyright 2018, Mary Ann Liebert, Inc. 2018
lds50peer_reviewed
oafree_for_read
citedbyFETCH-LOGICAL-1577t-f0a6d1376a67cbe17c09aaf8c00270a7a0bcd571344521c756366954d0ec01fe3
citesFETCH-LOGICAL-1577t-f0a6d1376a67cbe17c09aaf8c00270a7a0bcd571344521c756366954d0ec01fe3
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
thumbnail$$Usyndetics_thumb_exl
backlink$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29084441$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Nolan, David J.
1Rose, Rebecca
2Rodriguez, Patricia H.
3Salemi, Marco
4Singer, Elyse J.
5Lamers, Susanna L.
6McGrath, Michael S.
title
0The Spleen Is an HIV-1 Sanctuary During Combined Antiretroviral Therapy
1AIDS research and human retroviruses
addtitleAIDS Res Hum Retroviruses
descriptionCombined antiretroviral therapy (cART) does not eradicate HIV, which persists for years and can re-establish replication if treatment is stopped. The current challenge is identifying those tissues harboring virus through cART. Here, we used HIV env-nef single genome sequencing and HIV gag droplet digital PCR (ddPCR) to survey 50 tissues from five subjects on cART with no detectable plasma viral load at death. The spleen most consistently contained multiple proviral and expressed sequences (4/5 participants). Spleen-derived HIV demonstrated two distinct phylogenetic patterns: multiple identical sequences, often from different tissues, as well as diverse viral sequences on long terminal branches. Our results suggested that ddPCR may overestimate the size of the tissue-based viral reservoir. The spleen, a lymphatic organ at the intersection of the immune and circulatory systems, may play a key role in viral persistence.
subject
0Adult
1Aged
2Anti-Retroviral Agents - therapeutic use
3Antiretroviral agents
4Antiretroviral drugs
5Antiretroviral therapy
6Antiretroviral Therapy, Highly Active
7Drug therapy
8Gag protein
9Gene sequencing
10Genes, gag
11Genomes
12HIV
13HIV evolution
14HIV Infections - mortality
15HIV Infections - virology
16HIV Seropositivity
17HIV-1 - drug effects
18HIV-1 - genetics
19Human immunodeficiency virus
20Humans
21lymphatic system
22Male
23Middle Aged
24phylogenetics
25Phylogeny
26Polymerase Chain Reaction
27Proviruses - genetics
28RNA, Viral - blood
29Sequence Note
30Spleen
31Spleen - virology
32Therapy
33Tissues
34Viral Load
35Viral Proteins - genetics
36viral reservoirs
37virus diseases
38Viruses
issn
00889-2229
11931-8405
fulltexttrue
rsrctypearticle
creationdate2018
recordtypearticle
recordideNqNkcFvFCEUxonR2LV69Gom8eJl1gcDA1xMNlvtbtLEQ6tXwgDT0szCyjBN-t_LZGvTNjV64vB-38f33ofQewxLDEJ-1t4uCWC-BMLoC7TAssG1oMBeogUIIWtCiDxCb8bxGgAkIew1OiISBKUUL9DpxZWrzveDc6HajpUO1Wb7s8bVuQ4mTzrdVidT8uGyWsdd54Oz1Spkn1xO8cYnPVRFn_T-9i161ethdO_u3mP049vXi_WmPvt-ul2vzmrMOM91D7q1uOGtbrnpHOYGpNa9MACEg-YaOmMZxw2ljGDDWdu0rWTUgjOAe9ccoy8H3_3U7Zw1LuSSQu2T35WwKmqvHk-Cv1KX8UaV7zGjpBhsDgZx74IumzzS2uCyilaRlishWO86aXljetn3urOiYbJj2FiwAtNi9ekuS4q_JjdmtfOjccOgg4vTqLBkgtHSCy7oxyfodZxSKJcqlJjNQDSFqg-USXEck-vvs2FQc9uqtK3mttXcduE_PDzGPf2n3gI0TwyNzzr7ON_GD3-1_T_VanvynGqOoEMYvOtcyv9Y4TfpONZn
startdate20180101
enddate20180101
creator
0Nolan, David J.
1Rose, Rebecca
2Rodriguez, Patricia H.
3Salemi, Marco
4Singer, Elyse J.
5Lamers, Susanna L.
6McGrath, Michael S.
generalMary Ann Liebert, Inc
scope
0CGR
1CUY
2CVF
3ECM
4EIF
5NPM
6AAYXX
7CITATION
87QL
97T2
107T5
117T7
127TK
137U7
147U9
158FD
16C1K
17FR3
18H94
19M7N
20P64
217X8
22BOBZL
23CLFQK
245PM
sort
creationdate20180101
titleThe Spleen Is an HIV-1 Sanctuary During Combined Antiretroviral Therapy
authorNolan, David J. ; Rose, Rebecca ; Rodriguez, Patricia H. ; Salemi, Marco ; Singer, Elyse J. ; Lamers, Susanna L. ; McGrath, Michael S.
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1577t-f0a6d1376a67cbe17c09aaf8c00270a7a0bcd571344521c756366954d0ec01fe3
rsrctypearticles
prefilterarticles
languageeng
creationdate2018
topic
0Adult
1Aged
2Anti-Retroviral Agents - therapeutic use
3Antiretroviral agents
4Antiretroviral drugs
5Antiretroviral therapy
6Antiretroviral Therapy, Highly Active
7Drug therapy
8Gag protein
9Gene sequencing
10Genes, gag
11Genomes
12HIV
13HIV evolution
14HIV Infections - mortality
15HIV Infections - virology
16HIV Seropositivity
17HIV-1 - drug effects
18HIV-1 - genetics
19Human immunodeficiency virus
20Humans
21lymphatic system
22Male
23Middle Aged
24phylogenetics
25Phylogeny
26Polymerase Chain Reaction
27Proviruses - genetics
28RNA, Viral - blood
29Sequence Note
30Spleen
31Spleen - virology
32Therapy
33Tissues
34Viral Load
35Viral Proteins - genetics
36viral reservoirs
37virus diseases
38Viruses
toplevel
0peer_reviewed
1online_resources
creatorcontrib
0Nolan, David J.
1Rose, Rebecca
2Rodriguez, Patricia H.
3Salemi, Marco
4Singer, Elyse J.
5Lamers, Susanna L.
6McGrath, Michael S.
collection
0Medline
1MEDLINE
2MEDLINE (Ovid)
3MEDLINE
4MEDLINE
5PubMed
6CrossRef
7Bacteriology Abstracts (Microbiology B)
8Health and Safety Science Abstracts (Full archive)
9Immunology Abstracts
10Industrial and Applied Microbiology Abstracts (Microbiology A)
11Neurosciences Abstracts
12Toxicology Abstracts
13Virology and AIDS Abstracts
14Technology Research Database
15Environmental Sciences and Pollution Management
16Engineering Research Database
17AIDS and Cancer Research Abstracts
18Algology Mycology and Protozoology Abstracts (Microbiology C)
19Biotechnology and BioEngineering Abstracts
20MEDLINE - Academic
21OpenAIRE (Open Access)
22OpenAIRE
23PubMed Central (Full Participant titles)
jtitleAIDS research and human retroviruses
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Nolan, David J.
1Rose, Rebecca
2Rodriguez, Patricia H.
3Salemi, Marco
4Singer, Elyse J.
5Lamers, Susanna L.
6McGrath, Michael S.
formatjournal
genrearticle
ristypeJOUR
atitleThe Spleen Is an HIV-1 Sanctuary During Combined Antiretroviral Therapy
jtitleAIDS research and human retroviruses
addtitleAIDS Res Hum Retroviruses
date2018-01-01
risdate2018
volume34
issue1
spage123
epage125
pages123-125
issn0889-2229
eissn1931-8405
abstractCombined antiretroviral therapy (cART) does not eradicate HIV, which persists for years and can re-establish replication if treatment is stopped. The current challenge is identifying those tissues harboring virus through cART. Here, we used HIV env-nef single genome sequencing and HIV gag droplet digital PCR (ddPCR) to survey 50 tissues from five subjects on cART with no detectable plasma viral load at death. The spleen most consistently contained multiple proviral and expressed sequences (4/5 participants). Spleen-derived HIV demonstrated two distinct phylogenetic patterns: multiple identical sequences, often from different tissues, as well as diverse viral sequences on long terminal branches. Our results suggested that ddPCR may overestimate the size of the tissue-based viral reservoir. The spleen, a lymphatic organ at the intersection of the immune and circulatory systems, may play a key role in viral persistence.
copUnited States
pubMary Ann Liebert, Inc
pmid29084441
doi10.1089/aid.2017.0254
oafree_for_read