schliessen

Filtern

 

Bibliotheken

Screening and identification of T helper 1 and linear immunodominant antibody-binding epitopes in the spike 2 domain and the nucleocapsid protein of feline infectious peritonitis virus

Abstract The antibody-dependent enhancement (ADE) of feline infectious peritonitis virus (FIPV) infection has been recognized in experimentally infected cats, and cellular immunity is considered to play an important role in preventing the onset of feline infectious peritonitis (FIP). In the present... Full description

Journal Title: Vaccine 2011, Vol.29 (9), p.1791-1800
Main Author: Satoh, Ryoichi
Other Authors: Furukawa, Tomoko , Kotake, Masako , Takano, Tomomi , Motokawa, Kenji , Gemma, Tsuyoshi , Watanabe, Rie , Arai, Setsuo , Hohdatsu, Tsutomu
Format: Electronic Article Electronic Article
Language: English
Subjects:
FIP
Quelle: Alma/SFX Local Collection
Publisher: Kidlington: Elsevier Ltd
ID: ISSN: 0264-410X
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7115570
title: Screening and identification of T helper 1 and linear immunodominant antibody-binding epitopes in the spike 2 domain and the nucleocapsid protein of feline infectious peritonitis virus
format: Article
creator:
  • Satoh, Ryoichi
  • Furukawa, Tomoko
  • Kotake, Masako
  • Takano, Tomomi
  • Motokawa, Kenji
  • Gemma, Tsuyoshi
  • Watanabe, Rie
  • Arai, Setsuo
  • Hohdatsu, Tsutomu
subjects:
  • ADE, antibody-dependent enhancement
  • Allergy and Immunology
  • Amino Acid Sequence
  • Amino acids
  • Animals
  • Antibodies, Viral - immunology
  • Antibodies, Viral - metabolism
  • antibody
  • Applied microbiology
  • Article
  • binding epitope
  • Binding Sites, Antibody
  • Biological and medical sciences
  • Cats
  • Coronavirus
  • Coronavirus, Feline - immunology
  • Coronavirus, Feline - metabolism
  • dependent enhancement
  • Epitopes - immunology
  • Epitopes - metabolism
  • Epitopes, T-Lymphocyte - immunology
  • Epitopes, T-Lymphocyte - metabolism
  • FCoV
  • FCoV, feline coronavirus
  • FECV, feline enteric coronavirus
  • feline coronavirus
  • feline enteric coronavirus
  • feline infectious peritonitis
  • Feline Infectious Peritonitis - immunology
  • Feline Infectious Peritonitis - metabolism
  • Feline infectious peritonitis virus
  • FIP
  • FIP, feline infectious peritonitis
  • FIPV, feline infectious peritonitis virus
  • Fundamental and applied biological sciences. Psychology
  • Genetic Testing - methods
  • helical
  • heptad repeat
  • HR, heptad repeat
  • IH, inter-helical
  • Immunodominant Epitopes - immunology
  • Immunodominant Epitopes - metabolism
  • Infections
  • inter
  • Linear immunodominant antibody
  • Linear immunodominant antibody-binding epitope
  • Microbiology
  • Miscellaneous
  • Molecular Sequence Data
  • Nucleocapsid Proteins - immunology
  • Nucleocapsid Proteins - metabolism
  • PBMCs
  • PBMCs, peripheral blood mononuclear cells
  • Peptides
  • peripheral blood mononuclear cells
  • Protein Structure, Tertiary
  • Proteins
  • Th1 Cells - immunology
  • Th1 Cells - metabolism
  • Th1 epitope
  • Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
  • Virology
ispartof: Vaccine, 2011, Vol.29 (9), p.1791-1800
description: Abstract The antibody-dependent enhancement (ADE) of feline infectious peritonitis virus (FIPV) infection has been recognized in experimentally infected cats, and cellular immunity is considered to play an important role in preventing the onset of feline infectious peritonitis (FIP). In the present study, we synthesized eighty-one kinds of peptides derived from the spike (S)2 domain of type I FIPV KU-2 strain, the S2 domain of type II FIPV 79-1146 strain, and the nucleocapcid (N) protein of FIPV KU-2 strain. To detect the T helper (Th)1 epitope, peripheral blood mononuclear cells (PBMCs) obtained from FIPV-infected cats were cultured with each peptide, and Th1-type immune responses were measured using feline interferon (fIFN)-γ production as an index. To detect the linear immunodominant antibody-binding epitope, we investigated the reactivity of plasma collected from FIPV-infected cats against each peptide by ELISA. Four and 2 peptides containing Th1 epitopes were identified in the heptad repeat (HR)1 and inter-helical (IH) regions of the S2 domain of type I FIPV, respectively, and these were located on the N-terminal side of the regions. In the S2 domain of type II FIPV, 2, 3, and 2 peptides containing Th1 epitopes were identified in the HR1, IH, and HR2 regions, respectively, and these were mainly located on the C-terminal side of the regions. In the S2 domain of type I FIPV, 3 and 7 peptides containing linear immunodominant antibody-binding epitopes were identified in the IH and HR2 regions, respectively. In the S2 domain of type II FIPV, 4 peptides containing linear immunodominant antibody-binding epitopes were identified in the HR2 region. The Th1 epitopes in the S2 domain of type I and II FIPV were located in different regions, but the linear immunodominant antibody-binding epitopes were mostly located in the HR2 region. Eight peptides containing Th1 epitopes were identified in N protein, and 3 peptides derived from residues 81 to 100 and 137 to 164 showed strong inductivity of fIFN-γ production in PBMCs isolated from type I FIPV- and type II FIPV-infected non-FIP cats. In N protein, 4 peptides containing linear immunodominant antibody-binding epitopes were identified, and 2 peptides derived from residues 345 to 372 showed strong reactivity with plasma of type I FIPV- and type II FIPV-infected cats. The Th1 and linear immunodominant antibody-binding epitopes were located at different positions in both the S2 domain and N protein. Our results may prov
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0264-410X
fulltext: fulltext
issn:
  • 0264-410X
  • 1873-2518
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.3231027
LOCALfalse
PrimoNMBib
record
control
sourceidproquest_pubme
recordidTN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7115570
sourceformatXML
sourcesystemPC
sourcerecordid3218575221
originalsourceidFETCH-LOGICAL-1564t-907013dab118ac83368faf23ee9a08bd01e102ef01eccdf8ede5d451950da673
addsrcrecordideNqFU8Fu1DAQjRCIlsIngCwhxGkXjxM7yaUIVUCRKnFoD9wsrz1pZ5u1g52s1D_j83C6S0t76cnS-M2bN8_PRfEW-BI4qE_r5dZYSx6Xgs81kcvqWXEITV0uhITmeXHIhaoWFfBfB8WrlNacc1lC-7I4ECBAlSAOiz_nNiJ68pfMeMfIoR-pI2tGCp6Fjl2wK-wHjAxuAX2eaCKjzWbywYUNeePHfDPSKribxYq8m7lwoDEMmBh5Nl4hSwNdIxMsd5hcmpnmsp9sj8GaIZFjQwwj0u3QDuc5ublDm4VMiWUFmdHTSIltKU7pdfGiM33CN_vzqLj49vXi5HRx9vP7j5MvZwuQqhoXLa85lM6sABpjm7JUTWc6USK2hjcrxwGBC-zyaa3rGnQoXSWhldwZVZdHxfGOdphWG3Q22xNNr4dIGxNvdDCkH954utKXYatrAClrnglOdwTZDm8o4oNe53HUwWmhaq0cKIWSC2m7ymRRFW-Fw7Z2q5WroM1UH_daYvg9YRr1hpLFvjces0e6UVWr8rtWTyMll4o37bzf-0fIdZiiz45qqNoGgEsxT5Y7lI0hpYjd3RLA9RxHvdb7OOo5jhpELqvc9-5_9-66_uUvAz7sASZZ03fReEvpHlc2NQg5E33e4TA_9ZYw6mQJvUWXHbWjdoGelHL8iMHmjOWg99d4g-l-a52E5vp8_jvz1wHOoWlLKP8CTBkZHA
sourcetypeOpen Access Repository
isCDItrue
recordtypearticle
pqid1498110529
display
typearticle
titleScreening and identification of T helper 1 and linear immunodominant antibody-binding epitopes in the spike 2 domain and the nucleocapsid protein of feline infectious peritonitis virus
sourceAlma/SFX Local Collection
creatorSatoh, Ryoichi ; Furukawa, Tomoko ; Kotake, Masako ; Takano, Tomomi ; Motokawa, Kenji ; Gemma, Tsuyoshi ; Watanabe, Rie ; Arai, Setsuo ; Hohdatsu, Tsutomu
creatorcontribSatoh, Ryoichi ; Furukawa, Tomoko ; Kotake, Masako ; Takano, Tomomi ; Motokawa, Kenji ; Gemma, Tsuyoshi ; Watanabe, Rie ; Arai, Setsuo ; Hohdatsu, Tsutomu
descriptionAbstract The antibody-dependent enhancement (ADE) of feline infectious peritonitis virus (FIPV) infection has been recognized in experimentally infected cats, and cellular immunity is considered to play an important role in preventing the onset of feline infectious peritonitis (FIP). In the present study, we synthesized eighty-one kinds of peptides derived from the spike (S)2 domain of type I FIPV KU-2 strain, the S2 domain of type II FIPV 79-1146 strain, and the nucleocapcid (N) protein of FIPV KU-2 strain. To detect the T helper (Th)1 epitope, peripheral blood mononuclear cells (PBMCs) obtained from FIPV-infected cats were cultured with each peptide, and Th1-type immune responses were measured using feline interferon (fIFN)-γ production as an index. To detect the linear immunodominant antibody-binding epitope, we investigated the reactivity of plasma collected from FIPV-infected cats against each peptide by ELISA. Four and 2 peptides containing Th1 epitopes were identified in the heptad repeat (HR)1 and inter-helical (IH) regions of the S2 domain of type I FIPV, respectively, and these were located on the N-terminal side of the regions. In the S2 domain of type II FIPV, 2, 3, and 2 peptides containing Th1 epitopes were identified in the HR1, IH, and HR2 regions, respectively, and these were mainly located on the C-terminal side of the regions. In the S2 domain of type I FIPV, 3 and 7 peptides containing linear immunodominant antibody-binding epitopes were identified in the IH and HR2 regions, respectively. In the S2 domain of type II FIPV, 4 peptides containing linear immunodominant antibody-binding epitopes were identified in the HR2 region. The Th1 epitopes in the S2 domain of type I and II FIPV were located in different regions, but the linear immunodominant antibody-binding epitopes were mostly located in the HR2 region. Eight peptides containing Th1 epitopes were identified in N protein, and 3 peptides derived from residues 81 to 100 and 137 to 164 showed strong inductivity of fIFN-γ production in PBMCs isolated from type I FIPV- and type II FIPV-infected non-FIP cats. In N protein, 4 peptides containing linear immunodominant antibody-binding epitopes were identified, and 2 peptides derived from residues 345 to 372 showed strong reactivity with plasma of type I FIPV- and type II FIPV-infected cats. The Th1 and linear immunodominant antibody-binding epitopes were located at different positions in both the S2 domain and N protein. Our results may provide important information for the development of peptide-based vaccine against FIPV infection.
identifier
0ISSN: 0264-410X
1EISSN: 1873-2518
2DOI: 10.1016/j.vaccine.2010.12.106
3PMID: 21216312
4CODEN: VACCDE
languageeng
publisherKidlington: Elsevier Ltd
subjectADE, antibody-dependent enhancement ; Allergy and Immunology ; Amino Acid Sequence ; Amino acids ; Animals ; Antibodies, Viral - immunology ; Antibodies, Viral - metabolism ; antibody ; Applied microbiology ; Article ; binding epitope ; Binding Sites, Antibody ; Biological and medical sciences ; Cats ; Coronavirus ; Coronavirus, Feline - immunology ; Coronavirus, Feline - metabolism ; dependent enhancement ; Epitopes - immunology ; Epitopes - metabolism ; Epitopes, T-Lymphocyte - immunology ; Epitopes, T-Lymphocyte - metabolism ; FCoV ; FCoV, feline coronavirus ; FECV, feline enteric coronavirus ; feline coronavirus ; feline enteric coronavirus ; feline infectious peritonitis ; Feline Infectious Peritonitis - immunology ; Feline Infectious Peritonitis - metabolism ; Feline infectious peritonitis virus ; FIP ; FIP, feline infectious peritonitis ; FIPV, feline infectious peritonitis virus ; Fundamental and applied biological sciences. Psychology ; Genetic Testing - methods ; helical ; heptad repeat ; HR, heptad repeat ; IH, inter-helical ; Immunodominant Epitopes - immunology ; Immunodominant Epitopes - metabolism ; Infections ; inter ; Linear immunodominant antibody ; Linear immunodominant antibody-binding epitope ; Microbiology ; Miscellaneous ; Molecular Sequence Data ; Nucleocapsid Proteins - immunology ; Nucleocapsid Proteins - metabolism ; PBMCs ; PBMCs, peripheral blood mononuclear cells ; Peptides ; peripheral blood mononuclear cells ; Protein Structure, Tertiary ; Proteins ; Th1 Cells - immunology ; Th1 Cells - metabolism ; Th1 epitope ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) ; Virology
ispartofVaccine, 2011, Vol.29 (9), p.1791-1800
rights
0Elsevier Ltd
12011 Elsevier Ltd
22015 INIST-CNRS
3Copyright © 2011 Elsevier Ltd. All rights reserved.
4Copyright Elsevier Limited Feb 17, 2011
5Copyright © 2011 Elsevier Ltd. All rights reserved. 2011 Elsevier Ltd
lds50peer_reviewed
oafree_for_read
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
thumbnail$$Usyndetics_thumb_exl
backlink
0$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23871256$$DView record in Pascal Francis
1$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21216312$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Satoh, Ryoichi
1Furukawa, Tomoko
2Kotake, Masako
3Takano, Tomomi
4Motokawa, Kenji
5Gemma, Tsuyoshi
6Watanabe, Rie
7Arai, Setsuo
8Hohdatsu, Tsutomu
title
0Screening and identification of T helper 1 and linear immunodominant antibody-binding epitopes in the spike 2 domain and the nucleocapsid protein of feline infectious peritonitis virus
1Vaccine
addtitleVaccine
descriptionAbstract The antibody-dependent enhancement (ADE) of feline infectious peritonitis virus (FIPV) infection has been recognized in experimentally infected cats, and cellular immunity is considered to play an important role in preventing the onset of feline infectious peritonitis (FIP). In the present study, we synthesized eighty-one kinds of peptides derived from the spike (S)2 domain of type I FIPV KU-2 strain, the S2 domain of type II FIPV 79-1146 strain, and the nucleocapcid (N) protein of FIPV KU-2 strain. To detect the T helper (Th)1 epitope, peripheral blood mononuclear cells (PBMCs) obtained from FIPV-infected cats were cultured with each peptide, and Th1-type immune responses were measured using feline interferon (fIFN)-γ production as an index. To detect the linear immunodominant antibody-binding epitope, we investigated the reactivity of plasma collected from FIPV-infected cats against each peptide by ELISA. Four and 2 peptides containing Th1 epitopes were identified in the heptad repeat (HR)1 and inter-helical (IH) regions of the S2 domain of type I FIPV, respectively, and these were located on the N-terminal side of the regions. In the S2 domain of type II FIPV, 2, 3, and 2 peptides containing Th1 epitopes were identified in the HR1, IH, and HR2 regions, respectively, and these were mainly located on the C-terminal side of the regions. In the S2 domain of type I FIPV, 3 and 7 peptides containing linear immunodominant antibody-binding epitopes were identified in the IH and HR2 regions, respectively. In the S2 domain of type II FIPV, 4 peptides containing linear immunodominant antibody-binding epitopes were identified in the HR2 region. The Th1 epitopes in the S2 domain of type I and II FIPV were located in different regions, but the linear immunodominant antibody-binding epitopes were mostly located in the HR2 region. Eight peptides containing Th1 epitopes were identified in N protein, and 3 peptides derived from residues 81 to 100 and 137 to 164 showed strong inductivity of fIFN-γ production in PBMCs isolated from type I FIPV- and type II FIPV-infected non-FIP cats. In N protein, 4 peptides containing linear immunodominant antibody-binding epitopes were identified, and 2 peptides derived from residues 345 to 372 showed strong reactivity with plasma of type I FIPV- and type II FIPV-infected cats. The Th1 and linear immunodominant antibody-binding epitopes were located at different positions in both the S2 domain and N protein. Our results may provide important information for the development of peptide-based vaccine against FIPV infection.
subject
0ADE, antibody-dependent enhancement
1Allergy and Immunology
2Amino Acid Sequence
3Amino acids
4Animals
5Antibodies, Viral - immunology
6Antibodies, Viral - metabolism
7antibody
8Applied microbiology
9Article
10binding epitope
11Binding Sites, Antibody
12Biological and medical sciences
13Cats
14Coronavirus
15Coronavirus, Feline - immunology
16Coronavirus, Feline - metabolism
17dependent enhancement
18Epitopes - immunology
19Epitopes - metabolism
20Epitopes, T-Lymphocyte - immunology
21Epitopes, T-Lymphocyte - metabolism
22FCoV
23FCoV, feline coronavirus
24FECV, feline enteric coronavirus
25feline coronavirus
26feline enteric coronavirus
27feline infectious peritonitis
28Feline Infectious Peritonitis - immunology
29Feline Infectious Peritonitis - metabolism
30Feline infectious peritonitis virus
31FIP
32FIP, feline infectious peritonitis
33FIPV, feline infectious peritonitis virus
34Fundamental and applied biological sciences. Psychology
35Genetic Testing - methods
36helical
37heptad repeat
38HR, heptad repeat
39IH, inter-helical
40Immunodominant Epitopes - immunology
41Immunodominant Epitopes - metabolism
42Infections
43inter
44Linear immunodominant antibody
45Linear immunodominant antibody-binding epitope
46Microbiology
47Miscellaneous
48Molecular Sequence Data
49Nucleocapsid Proteins - immunology
50Nucleocapsid Proteins - metabolism
51PBMCs
52PBMCs, peripheral blood mononuclear cells
53Peptides
54peripheral blood mononuclear cells
55Protein Structure, Tertiary
56Proteins
57Th1 Cells - immunology
58Th1 Cells - metabolism
59Th1 epitope
60Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
61Virology
issn
00264-410X
11873-2518
fulltexttrue
rsrctypearticle
creationdate2011
recordtypearticle
recordideNqFU8Fu1DAQjRCIlsIngCwhxGkXjxM7yaUIVUCRKnFoD9wsrz1pZ5u1g52s1D_j83C6S0t76cnS-M2bN8_PRfEW-BI4qE_r5dZYSx6Xgs81kcvqWXEITV0uhITmeXHIhaoWFfBfB8WrlNacc1lC-7I4ECBAlSAOiz_nNiJ68pfMeMfIoR-pI2tGCp6Fjl2wK-wHjAxuAX2eaCKjzWbywYUNeePHfDPSKribxYq8m7lwoDEMmBh5Nl4hSwNdIxMsd5hcmpnmsp9sj8GaIZFjQwwj0u3QDuc5ublDm4VMiWUFmdHTSIltKU7pdfGiM33CN_vzqLj49vXi5HRx9vP7j5MvZwuQqhoXLa85lM6sABpjm7JUTWc6USK2hjcrxwGBC-zyaa3rGnQoXSWhldwZVZdHxfGOdphWG3Q22xNNr4dIGxNvdDCkH954utKXYatrAClrnglOdwTZDm8o4oNe53HUwWmhaq0cKIWSC2m7ymRRFW-Fw7Z2q5WroM1UH_daYvg9YRr1hpLFvjces0e6UVWr8rtWTyMll4o37bzf-0fIdZiiz45qqNoGgEsxT5Y7lI0hpYjd3RLA9RxHvdb7OOo5jhpELqvc9-5_9-66_uUvAz7sASZZ03fReEvpHlc2NQg5E33e4TA_9ZYw6mQJvUWXHbWjdoGelHL8iMHmjOWg99d4g-l-a52E5vp8_jvz1wHOoWlLKP8CTBkZHA
startdate2011
enddate2011
creator
0Satoh, Ryoichi
1Furukawa, Tomoko
2Kotake, Masako
3Takano, Tomomi
4Motokawa, Kenji
5Gemma, Tsuyoshi
6Watanabe, Rie
7Arai, Setsuo
8Hohdatsu, Tsutomu
general
0Elsevier Ltd
1Elsevier
2Elsevier Limited
scope
0IQODW
1CGR
2CUY
3CVF
4ECM
5EIF
6NPM
7AAYXX
8CITATION
93V.
107QL
117RV
127T2
137T5
147U9
157X7
167XB
1788C
1888E
198AO
208C1
218FE
228FH
238FI
248FJ
258FK
268G5
27ABUWG
28AZQEC
29BBNVY
30BENPR
31BHPHI
32C1K
33DWQXO
34FYUFA
35GHDGH
36GNUQQ
37GUQSH
38H94
39HCIFZ
40K9-
41K9.
42KB0
43LK8
44M0R
45M0S
46M0T
47M1P
48M2O
49M7N
50M7P
51MBDVC
52NAPCQ
53PQEST
54PQQKQ
55PQUKI
56Q9U
577X8
58BOBZL
59CLFQK
605PM
sort
creationdate2011
titleScreening and identification of T helper 1 and linear immunodominant antibody-binding epitopes in the spike 2 domain and the nucleocapsid protein of feline infectious peritonitis virus
authorSatoh, Ryoichi ; Furukawa, Tomoko ; Kotake, Masako ; Takano, Tomomi ; Motokawa, Kenji ; Gemma, Tsuyoshi ; Watanabe, Rie ; Arai, Setsuo ; Hohdatsu, Tsutomu
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1564t-907013dab118ac83368faf23ee9a08bd01e102ef01eccdf8ede5d451950da673
rsrctypearticles
prefilterarticles
languageeng
creationdate2011
topic
0ADE, antibody-dependent enhancement
1Allergy and Immunology
2Amino Acid Sequence
3Amino acids
4Animals
5Antibodies, Viral - immunology
6Antibodies, Viral - metabolism
7antibody
8Applied microbiology
9Article
10binding epitope
11Binding Sites, Antibody
12Biological and medical sciences
13Cats
14Coronavirus
15Coronavirus, Feline - immunology
16Coronavirus, Feline - metabolism
17dependent enhancement
18Epitopes - immunology
19Epitopes - metabolism
20Epitopes, T-Lymphocyte - immunology
21Epitopes, T-Lymphocyte - metabolism
22FCoV
23FCoV, feline coronavirus
24FECV, feline enteric coronavirus
25feline coronavirus
26feline enteric coronavirus
27feline infectious peritonitis
28Feline Infectious Peritonitis - immunology
29Feline Infectious Peritonitis - metabolism
30Feline infectious peritonitis virus
31FIP
32FIP, feline infectious peritonitis
33FIPV, feline infectious peritonitis virus
34Fundamental and applied biological sciences. Psychology
35Genetic Testing - methods
36helical
37heptad repeat
38HR, heptad repeat
39IH, inter-helical
40Immunodominant Epitopes - immunology
41Immunodominant Epitopes - metabolism
42Infections
43inter
44Linear immunodominant antibody
45Linear immunodominant antibody-binding epitope
46Microbiology
47Miscellaneous
48Molecular Sequence Data
49Nucleocapsid Proteins - immunology
50Nucleocapsid Proteins - metabolism
51PBMCs
52PBMCs, peripheral blood mononuclear cells
53Peptides
54peripheral blood mononuclear cells
55Protein Structure, Tertiary
56Proteins
57Th1 Cells - immunology
58Th1 Cells - metabolism
59Th1 epitope
60Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects)
61Virology
toplevel
0peer_reviewed
1online_resources
creatorcontrib
0Satoh, Ryoichi
1Furukawa, Tomoko
2Kotake, Masako
3Takano, Tomomi
4Motokawa, Kenji
5Gemma, Tsuyoshi
6Watanabe, Rie
7Arai, Setsuo
8Hohdatsu, Tsutomu
collection
0Pascal-Francis
1Medline
2MEDLINE
3MEDLINE (Ovid)
4MEDLINE
5MEDLINE
6PubMed
7CrossRef
8ProQuest Central (Corporate)
9Bacteriology Abstracts (Microbiology B)
10Nursing & Allied Health Database
11Health and Safety Science Abstracts (Full archive)
12Immunology Abstracts
13Virology and AIDS Abstracts
14Health & Medical Collection
15ProQuest Central (purchase pre-March 2016)
16Healthcare Administration Database (Alumni)
17Medical Database (Alumni Edition)
18ProQuest Pharma Collection
19Public Health Database
20ProQuest SciTech Collection
21ProQuest Natural Science Collection
22Hospital Premium Collection
23Hospital Premium Collection (Alumni Edition)
24ProQuest Central (Alumni) (purchase pre-March 2016)
25Research Library (Alumni Edition)
26ProQuest Central (Alumni Edition)
27ProQuest Central Essentials
28Biological Science Collection
29ProQuest Central
30Natural Science Collection
31Environmental Sciences and Pollution Management
32ProQuest Central Korea
33Health Research Premium Collection
34Health Research Premium Collection (Alumni)
35ProQuest Central Student
36Research Library Prep
37AIDS and Cancer Research Abstracts
38SciTech Premium Collection
39Consumer Health Database (Alumni Edition)
40ProQuest Health & Medical Complete (Alumni)
41Nursing & Allied Health Database (Alumni Edition)
42ProQuest Biological Science Collection
43Consumer Health Database
44Health & Medical Collection (Alumni Edition)
45Healthcare Administration Database
46Medical Database
47Research Library
48Algology Mycology and Protozoology Abstracts (Microbiology C)
49Biological Science Database
50Research Library (Corporate)
51Nursing & Allied Health Premium
52ProQuest One Academic Eastern Edition
53ProQuest One Academic
54ProQuest One Academic UKI Edition
55ProQuest Central Basic
56MEDLINE - Academic
57OpenAIRE (Open Access)
58OpenAIRE
59PubMed Central (Full Participant titles)
jtitleVaccine
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Satoh, Ryoichi
1Furukawa, Tomoko
2Kotake, Masako
3Takano, Tomomi
4Motokawa, Kenji
5Gemma, Tsuyoshi
6Watanabe, Rie
7Arai, Setsuo
8Hohdatsu, Tsutomu
formatjournal
genrearticle
ristypeJOUR
atitleScreening and identification of T helper 1 and linear immunodominant antibody-binding epitopes in the spike 2 domain and the nucleocapsid protein of feline infectious peritonitis virus
jtitleVaccine
addtitleVaccine
date2011
risdate2011
volume29
issue9
spage1791
epage1800
pages1791-1800
issn0264-410X
eissn1873-2518
codenVACCDE
abstractAbstract The antibody-dependent enhancement (ADE) of feline infectious peritonitis virus (FIPV) infection has been recognized in experimentally infected cats, and cellular immunity is considered to play an important role in preventing the onset of feline infectious peritonitis (FIP). In the present study, we synthesized eighty-one kinds of peptides derived from the spike (S)2 domain of type I FIPV KU-2 strain, the S2 domain of type II FIPV 79-1146 strain, and the nucleocapcid (N) protein of FIPV KU-2 strain. To detect the T helper (Th)1 epitope, peripheral blood mononuclear cells (PBMCs) obtained from FIPV-infected cats were cultured with each peptide, and Th1-type immune responses were measured using feline interferon (fIFN)-γ production as an index. To detect the linear immunodominant antibody-binding epitope, we investigated the reactivity of plasma collected from FIPV-infected cats against each peptide by ELISA. Four and 2 peptides containing Th1 epitopes were identified in the heptad repeat (HR)1 and inter-helical (IH) regions of the S2 domain of type I FIPV, respectively, and these were located on the N-terminal side of the regions. In the S2 domain of type II FIPV, 2, 3, and 2 peptides containing Th1 epitopes were identified in the HR1, IH, and HR2 regions, respectively, and these were mainly located on the C-terminal side of the regions. In the S2 domain of type I FIPV, 3 and 7 peptides containing linear immunodominant antibody-binding epitopes were identified in the IH and HR2 regions, respectively. In the S2 domain of type II FIPV, 4 peptides containing linear immunodominant antibody-binding epitopes were identified in the HR2 region. The Th1 epitopes in the S2 domain of type I and II FIPV were located in different regions, but the linear immunodominant antibody-binding epitopes were mostly located in the HR2 region. Eight peptides containing Th1 epitopes were identified in N protein, and 3 peptides derived from residues 81 to 100 and 137 to 164 showed strong inductivity of fIFN-γ production in PBMCs isolated from type I FIPV- and type II FIPV-infected non-FIP cats. In N protein, 4 peptides containing linear immunodominant antibody-binding epitopes were identified, and 2 peptides derived from residues 345 to 372 showed strong reactivity with plasma of type I FIPV- and type II FIPV-infected cats. The Th1 and linear immunodominant antibody-binding epitopes were located at different positions in both the S2 domain and N protein. Our results may provide important information for the development of peptide-based vaccine against FIPV infection.
copKidlington
pubElsevier Ltd
pmid21216312
doi10.1016/j.vaccine.2010.12.106
oafree_for_read