schliessen

Filtern

 

Bibliotheken

Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome

Summary Background Human infection with avian influenza A H7N9 virus emerged in eastern China in February, 2013, and has been associated with exposure to poultry. We report the clinical and microbiological features of patients infected with influenza A H7N9 virus and compare genomic features of the... Full description

Journal Title: Lancet (London England), 2013, Vol.381 (9881), p.1916-1925
Main Author: Chen, Yu, MD
Other Authors: Liang, Weifeng, MD , Yang, Shigui, PhD , Wu, Nanping, PhD , Gao, Hainv, MD , Sheng, Jifang, MD , Yao, Hangping, PhD , Wo, Jianer, PhD , Fang, Qiang, MD , Cui, Dawei, PhD , Li, Yongcheng, MD , Yao, Xing, MD , Zhang, Yuntao, MD , Wu, Haibo, PhD , Zheng, Shufa, PhD , Diao, Hongyan, PhD , Xia, Shichang, MD , Zhang, Yanjun, PhD , Chan, Kwok-Hung, PhD , Tsoi, Hoi-Wah, MPhil , Teng, Jade Lee-Lee, PhD , Song, Wenjun, PhD , Wang, Pui, PhD , Lau, Siu-Ying, MPhil , Zheng, Min, MPhil , Chan, Jasper Fuk-Woo, FRCPath , To, Kelvin Kai-Wang, FRCPath , Chen, Honglin, PhD , Li, Lanjuan, Prof , Yuen, Kwok-Yung, Prof
Format: Electronic Article Electronic Article
Language: English
Subjects:
Quelle: Alma/SFX Local Collection
Publisher: Kidlington: Elsevier Ltd
ID: ISSN: 0140-6736
Zum Text:
SendSend as email Add to Book BagAdd to Book Bag
Staff View
recordid: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7134567
title: Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome
format: Article
creator:
  • Chen, Yu, MD
  • Liang, Weifeng, MD
  • Yang, Shigui, PhD
  • Wu, Nanping, PhD
  • Gao, Hainv, MD
  • Sheng, Jifang, MD
  • Yao, Hangping, PhD
  • Wo, Jianer, PhD
  • Fang, Qiang, MD
  • Cui, Dawei, PhD
  • Li, Yongcheng, MD
  • Yao, Xing, MD
  • Zhang, Yuntao, MD
  • Wu, Haibo, PhD
  • Zheng, Shufa, PhD
  • Diao, Hongyan, PhD
  • Xia, Shichang, MD
  • Zhang, Yanjun, PhD
  • Chan, Kwok-Hung, PhD
  • Tsoi, Hoi-Wah, MPhil
  • Teng, Jade Lee-Lee, PhD
  • Song, Wenjun, PhD
  • Wang, Pui, PhD
  • Lau, Siu-Ying, MPhil
  • Zheng, Min, MPhil
  • Chan, Jasper Fuk-Woo, FRCPath
  • To, Kelvin Kai-Wang, FRCPath
  • Chen, Honglin, PhD
  • Li, Lanjuan, Prof
  • Yuen, Kwok-Yung, Prof
subjects:
  • Abridged Index Medicus
  • Animals
  • Article
  • Avian influenza
  • Biological and medical sciences
  • Birds
  • China
  • Colleges & universities
  • Communicable Diseases, Emerging - drug therapy
  • Communicable Diseases, Emerging - transmission
  • Communicable Diseases, Emerging - virology
  • Female
  • General aspects
  • Genetic aspects
  • Genome, Viral - genetics
  • Human viral diseases
  • Humans
  • Infectious diseases
  • Influenza A virus - classification
  • Influenza A virus - genetics
  • Influenza in Birds - genetics
  • Influenza in Birds - transmission
  • Influenza viruses
  • Influenza, Human - drug therapy
  • Influenza, Human - transmission
  • Influenza, Human - virology
  • Internal Medicine
  • Male
  • Medical sciences
  • Middle Aged
  • Physiological aspects
  • Poultry
  • Proteins
  • Respiratory distress syndrome
  • Risk factors
  • Tomography, X-Ray Computed
  • Viral diseases
  • Viral diseases of the respiratory system and ent viral diseases
  • Zoonoses - transmission
  • Zoonoses - virology
ispartof: Lancet (London, England), 2013, Vol.381 (9881), p.1916-1925
description: Summary Background Human infection with avian influenza A H7N9 virus emerged in eastern China in February, 2013, and has been associated with exposure to poultry. We report the clinical and microbiological features of patients infected with influenza A H7N9 virus and compare genomic features of the human virus with those of the virus in market poultry in Zhejiang, China. Methods Between March 7 and April 8, 2013, we included hospital inpatients if they had new-onset respiratory symptoms, unexplained radiographic infiltrate, and laboratory-confirmed H7N9 virus infection. We recorded histories and results of haematological, biochemical, radiological, and microbiological investigations. We took throat and sputum samples, used RT-PCR to detect M, H7, and N9 genes, and cultured samples in Madin-Darby canine kidney cells. We tested for co-infections and monitored serum concentrations of six cytokines and chemokines. We collected cloacal swabs from 86 birds from epidemiologically linked wet markets and inoculated embryonated chicken eggs with the samples. We identified and subtyped isolates by RT-PCR sequencing. RNA extraction, complementary DNA synthesis, and PCR sequencing were done for one human and one chicken isolate. We characterised and phylogenetically analysed the eight gene segments of the viruses in the patient's and the chicken's isolates, and constructed phylogenetic trees of H, N, PB2, and NS genes. Findings We identified four patients (mean age 56 years), all of whom had contact with poultry 3–8 days before disease onset. They presented with fever and rapidly progressive pneumonia that did not respond to antibiotics. Patients were leucopenic and lymphopenic, and had impaired liver or renal function, substantially increased serum cytokine or chemokine concentrations, and disseminated intravascular coagulation with disease progression. Two patients died. Sputum specimens were more likely to test positive for the H7N9 virus than were samples from throat swabs. The viral isolate from the patient was closely similar to that from an epidemiologically linked market chicken. All viral gene segments were of avian origin. The H7 of the isolated viruses was closest to that of the H7N3 virus from domestic ducks in Zhejiang, whereas the N9 was closest to that of the wild bird H7N9 virus in South Korea. We noted Gln226Leu and Gly186Val substitutions in human virus H7 (associated with increased affinity for α-2,6-linked sialic acid receptors) and the PB2 Asp701As
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0140-6736
fulltext: fulltext
issn:
  • 0140-6736
  • 1474-547X
url: Link


@attributes
NO1
SEARCH_ENGINEprimo_central_multiple_fe
SEARCH_ENGINE_TYPEPrimo Central Search Engine
RANK2.8257174
LOCALfalse
PrimoNMBib
record
control
sourceidgale_pubme
recordidTN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7134567
sourceformatXML
sourcesystemPC
galeidA341654386
sourcerecordidA341654386
originalsourceidFETCH-LOGICAL-1834t-bd09500d873910d6d95e851ae4b73c2c3f73376851e206704f385e41be70e1233
addsrcrecordideNqNk39r1DAYx4sobk5fghIQYYI3k-ZXqzAZQz1h6B8q-F9I06d3mW1yJu2N8034lk132203HJNCW9LP95vnyfNtlj0l-IBgIl5_xYThiZBU7BP6UuAS0wm7l-0SJtmEM_njfra7QXayRzGeYoyZwPxhtpNTkVNa4t3sz3TotEPWNWB6611EZ7afo34OCDoIM-tmSC_tGmkHcL81OkJT-blESxuGiJrgO3QGPep0-JkeCz-0fVi9Qaa1zhrdIu10u4o2ppcambkO2vQQbNTjfsg3o1HCZuB8B4-zB41uIzy5eO5l3z-8_3Y8nZx8-fjp-OhkQgrK-klV45JjXBeSlgTXoi45FJxoYJWkJje0kZRKkZYgx0Ji1tCCAyMVSAwk9b6XHa59F0PVQW3A9akItQg29bFSXlu1_cXZuZr5pZKEMp7OdC-brg38Apy2Aba0tYNe-VrlQqqyorIEwinNRaFFJTlueMO1wJXmkphktX9RS_C_Boi96mw00LbagR-iIlykTkpeyLtRKpgkmMsRfX4DPfVDSLMYqbQvkQXPr6iZbkGlGfvUrRlN1RFlRHBGC5Gog39Q6aqhs8Y7aGxa3xK8uiaohmgdxHSLdjbv40wPMW7jfI2b4GMM0GwOk2A1xl2dx12NWU7Vq_O4K5Z0z65PcaO6zHcCXlwAOqYsNkE7Y-MVJ2mRC14m7u2NAoztzwOaGrXtpox4Sxn_p76tiXdrNaS8Ly0EFY0FZ6BOqTK9qr290-HwhsPlz_cTVhCv5q5irvDaZPRIaRkdGP0LPc9Tfg
sourcetypeOpen Access Repository
isCDItrue
recordtypearticle
pqid1357117852
display
typearticle
titleHuman infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome
sourceAlma/SFX Local Collection
creatorChen, Yu, MD ; Liang, Weifeng, MD ; Yang, Shigui, PhD ; Wu, Nanping, PhD ; Gao, Hainv, MD ; Sheng, Jifang, MD ; Yao, Hangping, PhD ; Wo, Jianer, PhD ; Fang, Qiang, MD ; Cui, Dawei, PhD ; Li, Yongcheng, MD ; Yao, Xing, MD ; Zhang, Yuntao, MD ; Wu, Haibo, PhD ; Zheng, Shufa, PhD ; Diao, Hongyan, PhD ; Xia, Shichang, MD ; Zhang, Yanjun, PhD ; Chan, Kwok-Hung, PhD ; Tsoi, Hoi-Wah, MPhil ; Teng, Jade Lee-Lee, PhD ; Song, Wenjun, PhD ; Wang, Pui, PhD ; Lau, Siu-Ying, MPhil ; Zheng, Min, MPhil ; Chan, Jasper Fuk-Woo, FRCPath ; To, Kelvin Kai-Wang, FRCPath ; Chen, Honglin, PhD ; Li, Lanjuan, Prof ; Yuen, Kwok-Yung, Prof
creatorcontribChen, Yu, MD ; Liang, Weifeng, MD ; Yang, Shigui, PhD ; Wu, Nanping, PhD ; Gao, Hainv, MD ; Sheng, Jifang, MD ; Yao, Hangping, PhD ; Wo, Jianer, PhD ; Fang, Qiang, MD ; Cui, Dawei, PhD ; Li, Yongcheng, MD ; Yao, Xing, MD ; Zhang, Yuntao, MD ; Wu, Haibo, PhD ; Zheng, Shufa, PhD ; Diao, Hongyan, PhD ; Xia, Shichang, MD ; Zhang, Yanjun, PhD ; Chan, Kwok-Hung, PhD ; Tsoi, Hoi-Wah, MPhil ; Teng, Jade Lee-Lee, PhD ; Song, Wenjun, PhD ; Wang, Pui, PhD ; Lau, Siu-Ying, MPhil ; Zheng, Min, MPhil ; Chan, Jasper Fuk-Woo, FRCPath ; To, Kelvin Kai-Wang, FRCPath ; Chen, Honglin, PhD ; Li, Lanjuan, Prof ; Yuen, Kwok-Yung, Prof
descriptionSummary Background Human infection with avian influenza A H7N9 virus emerged in eastern China in February, 2013, and has been associated with exposure to poultry. We report the clinical and microbiological features of patients infected with influenza A H7N9 virus and compare genomic features of the human virus with those of the virus in market poultry in Zhejiang, China. Methods Between March 7 and April 8, 2013, we included hospital inpatients if they had new-onset respiratory symptoms, unexplained radiographic infiltrate, and laboratory-confirmed H7N9 virus infection. We recorded histories and results of haematological, biochemical, radiological, and microbiological investigations. We took throat and sputum samples, used RT-PCR to detect M, H7, and N9 genes, and cultured samples in Madin-Darby canine kidney cells. We tested for co-infections and monitored serum concentrations of six cytokines and chemokines. We collected cloacal swabs from 86 birds from epidemiologically linked wet markets and inoculated embryonated chicken eggs with the samples. We identified and subtyped isolates by RT-PCR sequencing. RNA extraction, complementary DNA synthesis, and PCR sequencing were done for one human and one chicken isolate. We characterised and phylogenetically analysed the eight gene segments of the viruses in the patient's and the chicken's isolates, and constructed phylogenetic trees of H, N, PB2, and NS genes. Findings We identified four patients (mean age 56 years), all of whom had contact with poultry 3–8 days before disease onset. They presented with fever and rapidly progressive pneumonia that did not respond to antibiotics. Patients were leucopenic and lymphopenic, and had impaired liver or renal function, substantially increased serum cytokine or chemokine concentrations, and disseminated intravascular coagulation with disease progression. Two patients died. Sputum specimens were more likely to test positive for the H7N9 virus than were samples from throat swabs. The viral isolate from the patient was closely similar to that from an epidemiologically linked market chicken. All viral gene segments were of avian origin. The H7 of the isolated viruses was closest to that of the H7N3 virus from domestic ducks in Zhejiang, whereas the N9 was closest to that of the wild bird H7N9 virus in South Korea. We noted Gln226Leu and Gly186Val substitutions in human virus H7 (associated with increased affinity for α-2,6-linked sialic acid receptors) and the PB2 Asp701Asn mutation (associated with mammalian adaptation). Ser31Asn mutation, which is associated with adamantane resistance, was noted in viral M2. Interpretation Cross species poultry-to-person transmission of this new reassortant H7N9 virus is associated with severe pneumonia and multiorgan dysfunction in human beings. Monitoring of the viral evolution and further study of disease pathogenesis will improve disease management, epidemic control, and pandemic preparedness. Funding Larry Chi-Kin Yung, National Key Program for Infectious Diseases of China.
identifier
0ISSN: 0140-6736
1EISSN: 1474-547X
2DOI: 10.1016/S0140-6736(13)60903-4
3PMID: 23623390
4CODEN: LANCAO
languageeng
publisherKidlington: Elsevier Ltd
subjectAbridged Index Medicus ; Animals ; Article ; Avian influenza ; Biological and medical sciences ; Birds ; China ; Colleges & universities ; Communicable Diseases, Emerging - drug therapy ; Communicable Diseases, Emerging - transmission ; Communicable Diseases, Emerging - virology ; Female ; General aspects ; Genetic aspects ; Genome, Viral - genetics ; Human viral diseases ; Humans ; Infectious diseases ; Influenza A virus - classification ; Influenza A virus - genetics ; Influenza in Birds - genetics ; Influenza in Birds - transmission ; Influenza viruses ; Influenza, Human - drug therapy ; Influenza, Human - transmission ; Influenza, Human - virology ; Internal Medicine ; Male ; Medical sciences ; Middle Aged ; Physiological aspects ; Poultry ; Proteins ; Respiratory distress syndrome ; Risk factors ; Tomography, X-Ray Computed ; Viral diseases ; Viral diseases of the respiratory system and ent viral diseases ; Zoonoses - transmission ; Zoonoses - virology
ispartofLancet (London, England), 2013, Vol.381 (9881), p.1916-1925
rights
0Elsevier Ltd
12013 Elsevier Ltd
22014 INIST-CNRS
3Copyright © 2013 Elsevier Ltd. All rights reserved.
4COPYRIGHT 2013 Elsevier B.V.
5Copyright Elsevier Limited Jun 1, 2013
6Copyright © 2013 Elsevier Ltd. All rights reserved. 2013 Elsevier Ltd
lds50peer_reviewed
oafree_for_read
citedbyFETCH-LOGICAL-1834t-bd09500d873910d6d95e851ae4b73c2c3f73376851e206704f385e41be70e1233
links
openurl$$Topenurl_article
openurlfulltext$$Topenurlfull_article
thumbnail$$Usyndetics_thumb_exl
backlink
0$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27382659$$DView record in Pascal Francis
1$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23623390$$D View this record in MEDLINE/PubMed
search
creatorcontrib
0Chen, Yu, MD
1Liang, Weifeng, MD
2Yang, Shigui, PhD
3Wu, Nanping, PhD
4Gao, Hainv, MD
5Sheng, Jifang, MD
6Yao, Hangping, PhD
7Wo, Jianer, PhD
8Fang, Qiang, MD
9Cui, Dawei, PhD
10Li, Yongcheng, MD
11Yao, Xing, MD
12Zhang, Yuntao, MD
13Wu, Haibo, PhD
14Zheng, Shufa, PhD
15Diao, Hongyan, PhD
16Xia, Shichang, MD
17Zhang, Yanjun, PhD
18Chan, Kwok-Hung, PhD
19Tsoi, Hoi-Wah, MPhil
20Teng, Jade Lee-Lee, PhD
21Song, Wenjun, PhD
22Wang, Pui, PhD
23Lau, Siu-Ying, MPhil
24Zheng, Min, MPhil
25Chan, Jasper Fuk-Woo, FRCPath
26To, Kelvin Kai-Wang, FRCPath
27Chen, Honglin, PhD
28Li, Lanjuan, Prof
29Yuen, Kwok-Yung, Prof
title
0Human infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome
1Lancet (London, England)
addtitleLancet
descriptionSummary Background Human infection with avian influenza A H7N9 virus emerged in eastern China in February, 2013, and has been associated with exposure to poultry. We report the clinical and microbiological features of patients infected with influenza A H7N9 virus and compare genomic features of the human virus with those of the virus in market poultry in Zhejiang, China. Methods Between March 7 and April 8, 2013, we included hospital inpatients if they had new-onset respiratory symptoms, unexplained radiographic infiltrate, and laboratory-confirmed H7N9 virus infection. We recorded histories and results of haematological, biochemical, radiological, and microbiological investigations. We took throat and sputum samples, used RT-PCR to detect M, H7, and N9 genes, and cultured samples in Madin-Darby canine kidney cells. We tested for co-infections and monitored serum concentrations of six cytokines and chemokines. We collected cloacal swabs from 86 birds from epidemiologically linked wet markets and inoculated embryonated chicken eggs with the samples. We identified and subtyped isolates by RT-PCR sequencing. RNA extraction, complementary DNA synthesis, and PCR sequencing were done for one human and one chicken isolate. We characterised and phylogenetically analysed the eight gene segments of the viruses in the patient's and the chicken's isolates, and constructed phylogenetic trees of H, N, PB2, and NS genes. Findings We identified four patients (mean age 56 years), all of whom had contact with poultry 3–8 days before disease onset. They presented with fever and rapidly progressive pneumonia that did not respond to antibiotics. Patients were leucopenic and lymphopenic, and had impaired liver or renal function, substantially increased serum cytokine or chemokine concentrations, and disseminated intravascular coagulation with disease progression. Two patients died. Sputum specimens were more likely to test positive for the H7N9 virus than were samples from throat swabs. The viral isolate from the patient was closely similar to that from an epidemiologically linked market chicken. All viral gene segments were of avian origin. The H7 of the isolated viruses was closest to that of the H7N3 virus from domestic ducks in Zhejiang, whereas the N9 was closest to that of the wild bird H7N9 virus in South Korea. We noted Gln226Leu and Gly186Val substitutions in human virus H7 (associated with increased affinity for α-2,6-linked sialic acid receptors) and the PB2 Asp701Asn mutation (associated with mammalian adaptation). Ser31Asn mutation, which is associated with adamantane resistance, was noted in viral M2. Interpretation Cross species poultry-to-person transmission of this new reassortant H7N9 virus is associated with severe pneumonia and multiorgan dysfunction in human beings. Monitoring of the viral evolution and further study of disease pathogenesis will improve disease management, epidemic control, and pandemic preparedness. Funding Larry Chi-Kin Yung, National Key Program for Infectious Diseases of China.
subject
0Abridged Index Medicus
1Animals
2Article
3Avian influenza
4Biological and medical sciences
5Birds
6China
7Colleges & universities
8Communicable Diseases, Emerging - drug therapy
9Communicable Diseases, Emerging - transmission
10Communicable Diseases, Emerging - virology
11Female
12General aspects
13Genetic aspects
14Genome, Viral - genetics
15Human viral diseases
16Humans
17Infectious diseases
18Influenza A virus - classification
19Influenza A virus - genetics
20Influenza in Birds - genetics
21Influenza in Birds - transmission
22Influenza viruses
23Influenza, Human - drug therapy
24Influenza, Human - transmission
25Influenza, Human - virology
26Internal Medicine
27Male
28Medical sciences
29Middle Aged
30Physiological aspects
31Poultry
32Proteins
33Respiratory distress syndrome
34Risk factors
35Tomography, X-Ray Computed
36Viral diseases
37Viral diseases of the respiratory system and ent viral diseases
38Zoonoses - transmission
39Zoonoses - virology
issn
00140-6736
11474-547X
fulltexttrue
rsrctypearticle
creationdate2013
recordtypearticle
recordideNqNk39r1DAYx4sobk5fghIQYYI3k-ZXqzAZQz1h6B8q-F9I06d3mW1yJu2N8034lk132203HJNCW9LP95vnyfNtlj0l-IBgIl5_xYThiZBU7BP6UuAS0wm7l-0SJtmEM_njfra7QXayRzGeYoyZwPxhtpNTkVNa4t3sz3TotEPWNWB6611EZ7afo34OCDoIM-tmSC_tGmkHcL81OkJT-blESxuGiJrgO3QGPep0-JkeCz-0fVi9Qaa1zhrdIu10u4o2ppcambkO2vQQbNTjfsg3o1HCZuB8B4-zB41uIzy5eO5l3z-8_3Y8nZx8-fjp-OhkQgrK-klV45JjXBeSlgTXoi45FJxoYJWkJje0kZRKkZYgx0Ji1tCCAyMVSAwk9b6XHa59F0PVQW3A9akItQg29bFSXlu1_cXZuZr5pZKEMp7OdC-brg38Apy2Aba0tYNe-VrlQqqyorIEwinNRaFFJTlueMO1wJXmkphktX9RS_C_Boi96mw00LbagR-iIlykTkpeyLtRKpgkmMsRfX4DPfVDSLMYqbQvkQXPr6iZbkGlGfvUrRlN1RFlRHBGC5Gog39Q6aqhs8Y7aGxa3xK8uiaohmgdxHSLdjbv40wPMW7jfI2b4GMM0GwOk2A1xl2dx12NWU7Vq_O4K5Z0z65PcaO6zHcCXlwAOqYsNkE7Y-MVJ2mRC14m7u2NAoztzwOaGrXtpox4Sxn_p76tiXdrNaS8Ly0EFY0FZ6BOqTK9qr290-HwhsPlz_cTVhCv5q5irvDaZPRIaRkdGP0LPc9Tfg
startdate2013
enddate2013
creator
0Chen, Yu, MD
1Liang, Weifeng, MD
2Yang, Shigui, PhD
3Wu, Nanping, PhD
4Gao, Hainv, MD
5Sheng, Jifang, MD
6Yao, Hangping, PhD
7Wo, Jianer, PhD
8Fang, Qiang, MD
9Cui, Dawei, PhD
10Li, Yongcheng, MD
11Yao, Xing, MD
12Zhang, Yuntao, MD
13Wu, Haibo, PhD
14Zheng, Shufa, PhD
15Diao, Hongyan, PhD
16Xia, Shichang, MD
17Zhang, Yanjun, PhD
18Chan, Kwok-Hung, PhD
19Tsoi, Hoi-Wah, MPhil
20Teng, Jade Lee-Lee, PhD
21Song, Wenjun, PhD
22Wang, Pui, PhD
23Lau, Siu-Ying, MPhil
24Zheng, Min, MPhil
25Chan, Jasper Fuk-Woo, FRCPath
26To, Kelvin Kai-Wang, FRCPath
27Chen, Honglin, PhD
28Li, Lanjuan, Prof
29Yuen, Kwok-Yung, Prof
general
0Elsevier Ltd
1Elsevier
2Elsevier B.V
3Elsevier Limited
scope
0IQODW
1CGR
2CUY
3CVF
4ECM
5EIF
6NPM
7AAYXX
8CITATION
9BSHEE
100TT
110TZ
120U~
133V.
147QL
157QP
167RV
177TK
187U7
197U9
207X7
217XB
2288A
2388C
2488E
2588G
2688I
278AF
288AO
298C1
308C2
318FE
328FH
338FI
348FJ
358FK
368G5
37ABUWG
38AN0
39ASE
40AZQEC
41BBNVY
42BEC
43BENPR
44BHPHI
45C1K
46DWQXO
47FPQ
48FYUFA
49GHDGH
50GNUQQ
51GUQSH
52H94
53HCIFZ
54K6X
55K9-
56K9.
57KB0
58KB~
59LK8
60M0R
61M0S
62M0T
63M1P
64M2M
65M2O
66M2P
67M7N
68M7P
69MBDVC
70NAPCQ
71PQEST
72PQQKQ
73PQUKI
74Q9U
75S0X
767X8
777T2
787U2
79BOBZL
80CLFQK
815PM
sort
creationdate2013
titleHuman infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome
authorChen, Yu, MD ; Liang, Weifeng, MD ; Yang, Shigui, PhD ; Wu, Nanping, PhD ; Gao, Hainv, MD ; Sheng, Jifang, MD ; Yao, Hangping, PhD ; Wo, Jianer, PhD ; Fang, Qiang, MD ; Cui, Dawei, PhD ; Li, Yongcheng, MD ; Yao, Xing, MD ; Zhang, Yuntao, MD ; Wu, Haibo, PhD ; Zheng, Shufa, PhD ; Diao, Hongyan, PhD ; Xia, Shichang, MD ; Zhang, Yanjun, PhD ; Chan, Kwok-Hung, PhD ; Tsoi, Hoi-Wah, MPhil ; Teng, Jade Lee-Lee, PhD ; Song, Wenjun, PhD ; Wang, Pui, PhD ; Lau, Siu-Ying, MPhil ; Zheng, Min, MPhil ; Chan, Jasper Fuk-Woo, FRCPath ; To, Kelvin Kai-Wang, FRCPath ; Chen, Honglin, PhD ; Li, Lanjuan, Prof ; Yuen, Kwok-Yung, Prof
facets
frbrtype5
frbrgroupidcdi_FETCH-LOGICAL-1834t-bd09500d873910d6d95e851ae4b73c2c3f73376851e206704f385e41be70e1233
rsrctypearticles
prefilterarticles
languageeng
creationdate2013
topic
0Abridged Index Medicus
1Animals
2Article
3Avian influenza
4Biological and medical sciences
5Birds
6China
7Colleges & universities
8Communicable Diseases, Emerging - drug therapy
9Communicable Diseases, Emerging - transmission
10Communicable Diseases, Emerging - virology
11Female
12General aspects
13Genetic aspects
14Genome, Viral - genetics
15Human viral diseases
16Humans
17Infectious diseases
18Influenza A virus - classification
19Influenza A virus - genetics
20Influenza in Birds - genetics
21Influenza in Birds - transmission
22Influenza viruses
23Influenza, Human - drug therapy
24Influenza, Human - transmission
25Influenza, Human - virology
26Internal Medicine
27Male
28Medical sciences
29Middle Aged
30Physiological aspects
31Poultry
32Proteins
33Respiratory distress syndrome
34Risk factors
35Tomography, X-Ray Computed
36Viral diseases
37Viral diseases of the respiratory system and ent viral diseases
38Zoonoses - transmission
39Zoonoses - virology
toplevel
0peer_reviewed
1online_resources
creatorcontrib
0Chen, Yu, MD
1Liang, Weifeng, MD
2Yang, Shigui, PhD
3Wu, Nanping, PhD
4Gao, Hainv, MD
5Sheng, Jifang, MD
6Yao, Hangping, PhD
7Wo, Jianer, PhD
8Fang, Qiang, MD
9Cui, Dawei, PhD
10Li, Yongcheng, MD
11Yao, Xing, MD
12Zhang, Yuntao, MD
13Wu, Haibo, PhD
14Zheng, Shufa, PhD
15Diao, Hongyan, PhD
16Xia, Shichang, MD
17Zhang, Yanjun, PhD
18Chan, Kwok-Hung, PhD
19Tsoi, Hoi-Wah, MPhil
20Teng, Jade Lee-Lee, PhD
21Song, Wenjun, PhD
22Wang, Pui, PhD
23Lau, Siu-Ying, MPhil
24Zheng, Min, MPhil
25Chan, Jasper Fuk-Woo, FRCPath
26To, Kelvin Kai-Wang, FRCPath
27Chen, Honglin, PhD
28Li, Lanjuan, Prof
29Yuen, Kwok-Yung, Prof
collection
0Pascal-Francis
1Medline
2MEDLINE
3MEDLINE (Ovid)
4MEDLINE
5MEDLINE
6PubMed
7CrossRef
8Academic OneFile (A&I only)
9News PRO
10Pharma and Biotech Premium PRO
11Global News & ABI/Inform Professional
12ProQuest Central (Corporate)
13Bacteriology Abstracts (Microbiology B)
14Calcium & Calcified Tissue Abstracts
15Nursing & Allied Health Database
16Neurosciences Abstracts
17Toxicology Abstracts
18Virology and AIDS Abstracts
19Health & Medical Collection
20ProQuest Central (purchase pre-March 2016)
21Biology Database (Alumni Edition)
22Healthcare Administration Database (Alumni)
23Medical Database (Alumni Edition)
24Psychology Database (Alumni)
25Science Database (Alumni Edition)
26STEM Database
27ProQuest Pharma Collection
28Public Health Database
29Lancet Titles
30ProQuest SciTech Collection
31ProQuest Natural Science Collection
32Hospital Premium Collection
33Hospital Premium Collection (Alumni Edition)
34ProQuest Central (Alumni) (purchase pre-March 2016)
35Research Library (Alumni Edition)
36ProQuest Central (Alumni Edition)
37British Nursing Database
38British Nursing Index
39ProQuest Central Essentials
40Biological Science Collection
41eLibrary
42ProQuest Central
43Natural Science Collection
44Environmental Sciences and Pollution Management
45ProQuest Central Korea
46British Nursing Index (BNI) (1985 to Present)
47Health Research Premium Collection
48Health Research Premium Collection (Alumni)
49ProQuest Central Student
50Research Library Prep
51AIDS and Cancer Research Abstracts
52SciTech Premium Collection
53British Nursing Index
54Consumer Health Database (Alumni Edition)
55ProQuest Health & Medical Complete (Alumni)
56Nursing & Allied Health Database (Alumni Edition)
57ProQuest Newsstand Professional
58ProQuest Biological Science Collection
59Consumer Health Database
60Health & Medical Collection (Alumni Edition)
61Healthcare Administration Database
62Medical Database
63Psychology Database
64Research Library
65Science Database
66Algology Mycology and Protozoology Abstracts (Microbiology C)
67Biological Science Database
68Research Library (Corporate)
69Nursing & Allied Health Premium
70ProQuest One Academic Eastern Edition
71ProQuest One Academic
72ProQuest One Academic UKI Edition
73ProQuest Central Basic
74SIRS Editorial
75MEDLINE - Academic
76Health and Safety Science Abstracts (Full archive)
77Safety Science and Risk
78OpenAIRE (Open Access)
79OpenAIRE
80PubMed Central (Full Participant titles)
jtitleLancet (London, England)
delivery
delcategoryRemote Search Resource
fulltextfulltext
addata
au
0Chen, Yu, MD
1Liang, Weifeng, MD
2Yang, Shigui, PhD
3Wu, Nanping, PhD
4Gao, Hainv, MD
5Sheng, Jifang, MD
6Yao, Hangping, PhD
7Wo, Jianer, PhD
8Fang, Qiang, MD
9Cui, Dawei, PhD
10Li, Yongcheng, MD
11Yao, Xing, MD
12Zhang, Yuntao, MD
13Wu, Haibo, PhD
14Zheng, Shufa, PhD
15Diao, Hongyan, PhD
16Xia, Shichang, MD
17Zhang, Yanjun, PhD
18Chan, Kwok-Hung, PhD
19Tsoi, Hoi-Wah, MPhil
20Teng, Jade Lee-Lee, PhD
21Song, Wenjun, PhD
22Wang, Pui, PhD
23Lau, Siu-Ying, MPhil
24Zheng, Min, MPhil
25Chan, Jasper Fuk-Woo, FRCPath
26To, Kelvin Kai-Wang, FRCPath
27Chen, Honglin, PhD
28Li, Lanjuan, Prof
29Yuen, Kwok-Yung, Prof
formatjournal
genrearticle
ristypeJOUR
atitleHuman infections with the emerging avian influenza A H7N9 virus from wet market poultry: clinical analysis and characterisation of viral genome
jtitleLancet (London, England)
addtitleLancet
date2013
risdate2013
volume381
issue9881
spage1916
epage1925
pages1916-1925
issn0140-6736
eissn1474-547X
codenLANCAO
notesThese authors contributed equally to this work
abstractSummary Background Human infection with avian influenza A H7N9 virus emerged in eastern China in February, 2013, and has been associated with exposure to poultry. We report the clinical and microbiological features of patients infected with influenza A H7N9 virus and compare genomic features of the human virus with those of the virus in market poultry in Zhejiang, China. Methods Between March 7 and April 8, 2013, we included hospital inpatients if they had new-onset respiratory symptoms, unexplained radiographic infiltrate, and laboratory-confirmed H7N9 virus infection. We recorded histories and results of haematological, biochemical, radiological, and microbiological investigations. We took throat and sputum samples, used RT-PCR to detect M, H7, and N9 genes, and cultured samples in Madin-Darby canine kidney cells. We tested for co-infections and monitored serum concentrations of six cytokines and chemokines. We collected cloacal swabs from 86 birds from epidemiologically linked wet markets and inoculated embryonated chicken eggs with the samples. We identified and subtyped isolates by RT-PCR sequencing. RNA extraction, complementary DNA synthesis, and PCR sequencing were done for one human and one chicken isolate. We characterised and phylogenetically analysed the eight gene segments of the viruses in the patient's and the chicken's isolates, and constructed phylogenetic trees of H, N, PB2, and NS genes. Findings We identified four patients (mean age 56 years), all of whom had contact with poultry 3–8 days before disease onset. They presented with fever and rapidly progressive pneumonia that did not respond to antibiotics. Patients were leucopenic and lymphopenic, and had impaired liver or renal function, substantially increased serum cytokine or chemokine concentrations, and disseminated intravascular coagulation with disease progression. Two patients died. Sputum specimens were more likely to test positive for the H7N9 virus than were samples from throat swabs. The viral isolate from the patient was closely similar to that from an epidemiologically linked market chicken. All viral gene segments were of avian origin. The H7 of the isolated viruses was closest to that of the H7N3 virus from domestic ducks in Zhejiang, whereas the N9 was closest to that of the wild bird H7N9 virus in South Korea. We noted Gln226Leu and Gly186Val substitutions in human virus H7 (associated with increased affinity for α-2,6-linked sialic acid receptors) and the PB2 Asp701Asn mutation (associated with mammalian adaptation). Ser31Asn mutation, which is associated with adamantane resistance, was noted in viral M2. Interpretation Cross species poultry-to-person transmission of this new reassortant H7N9 virus is associated with severe pneumonia and multiorgan dysfunction in human beings. Monitoring of the viral evolution and further study of disease pathogenesis will improve disease management, epidemic control, and pandemic preparedness. Funding Larry Chi-Kin Yung, National Key Program for Infectious Diseases of China.
copKidlington
pubElsevier Ltd
pmid23623390
doi10.1016/S0140-6736(13)60903-4
oafree_for_read