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Comparative metabolomics of muscle interstitium fluid in human trapezius myalgia: an in vivo microdialysis study

Purpose The mechanisms behind trapezius myalgia are unclear. Many hypotheses have been presented suggesting an altered metabolism in the muscle. Here, muscle microdialysate from healthy and myalgic muscle is analysed using metabolomics. Metabolomics analyse a vast number of metabolites, enabling a c... Full description

Journal Title: European journal of applied physiology 2013, Vol.113 (12), p.2977-2989
Main Author: Hadrévi, J
Other Authors: Ghafouri, B , Sjörs, A , Antti, H , Larsson, B , Crenshaw, A. G , Gerdle, B , Hellström, F
Format: Electronic Article Electronic Article
Language: English
Subjects:
Publisher: Berlin/Heidelberg: Springer Berlin Heidelberg
ID: ISSN: 1439-6319
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title: Comparative metabolomics of muscle interstitium fluid in human trapezius myalgia: an in vivo microdialysis study
format: Article
creator:
  • Hadrévi, J
  • Ghafouri, B
  • Sjörs, A
  • Antti, H
  • Larsson, B
  • Crenshaw, A. G
  • Gerdle, B
  • Hellström, F
subjects:
  • Adult
  • anatomi
  • Annan medicinsk grundvetenskap
  • Basic Medicine
  • Biomedical and Life Sciences
  • Biomedicine
  • Case-Control Studies
  • Environmental
  • Exercise
  • Extracellular Fluid - metabolism
  • Fatty Acids - metabolism
  • Female
  • Fysiologi
  • GC-MS
  • Human Anatomy
  • Human Physiology
  • Humans
  • Leucine - metabolism
  • Medical and Health Sciences
  • MEDICIN
  • Medicin och hälsovetenskap
  • MEDICINE
  • Medicinska och farmaceutiska grundvetenskaper
  • Metabolites
  • Metabolome
  • Metabolomics
  • Metabolomics
  • Trapezius myalgia
  • Microdialysis
  • Repetitive work
  • Recovery
  • GC-MS
  • Metabolites
  • Microdialysis
  • Muscle, Skeletal - metabolism
  • Muscle, Skeletal - pathology
  • musculoskeletal diseases
  • Myalgia - metabolism
  • Myristic Acid
  • Occupational Health
  • Occupational Medicine/Industrial Medicine
  • Original
  • Original Article
  • Orthopedics
  • Other Basic Medicine
  • Physiology
  • Physiology (medical)
  • Public Health
  • Putrescine - metabolism
  • Pyrrolidonecarboxylic Acid - metabolism
  • Recovery
  • Repetitive work
  • Sports Medicine
  • Trapezius myalgia
ispartof: European journal of applied physiology, 2013, Vol.113 (12), p.2977-2989
description: Purpose The mechanisms behind trapezius myalgia are unclear. Many hypotheses have been presented suggesting an altered metabolism in the muscle. Here, muscle microdialysate from healthy and myalgic muscle is analysed using metabolomics. Metabolomics analyse a vast number of metabolites, enabling a comprehensive explorative screening of the cellular processes in the muscle. Methods Microdialysate samples were obtained from the shoulder muscle of healthy and myalgic subjects that performed a work and stress test. Samples from the baseline period and from the recovery period were analysed using gas chromatography—mass spectrometry (GC–MS) together with multivariate analysis to detect differences in extracellular content of metabolites between groups. Systematic differences in metabolites between groups were identified using multivariate analysis and orthogonal partial least square discriminate analysis (OPLS-DA). A complementary Mann–Whitney U test of group difference in individual metabolites was also performed. Results A large number of metabolites were detected and identified in this screening study. At baseline, no systematic differences between groups were observed according to the OPLS-DA. However, two metabolites, l -leucine and pyroglutamic acid, were significantly more abundant in the myalgic muscle compared to the healthy muscle. In the recovery period, systematic difference in metabolites between the groups was observed according to the OPLS-DA. The groups differed in amino acids, fatty acids and carbohydrates. Myristic acid and putrescine were significantly more abundant and beta- d -glucopyranose was significantly less abundant in the myalgic muscle. Conclusion This study provides important information regarding the metabolite content, thereby presenting new clues regarding the pathophysiology of the myalgic muscle.
language: eng
source:
identifier: ISSN: 1439-6319
fulltext: no_fulltext
issn:
  • 1439-6319
  • 1439-6327
  • 1439-6327
url: Link


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titleComparative metabolomics of muscle interstitium fluid in human trapezius myalgia: an in vivo microdialysis study
creatorHadrévi, J ; Ghafouri, B ; Sjörs, A ; Antti, H ; Larsson, B ; Crenshaw, A. G ; Gerdle, B ; Hellström, F
creatorcontribHadrévi, J ; Ghafouri, B ; Sjörs, A ; Antti, H ; Larsson, B ; Crenshaw, A. G ; Gerdle, B ; Hellström, F
descriptionPurpose The mechanisms behind trapezius myalgia are unclear. Many hypotheses have been presented suggesting an altered metabolism in the muscle. Here, muscle microdialysate from healthy and myalgic muscle is analysed using metabolomics. Metabolomics analyse a vast number of metabolites, enabling a comprehensive explorative screening of the cellular processes in the muscle. Methods Microdialysate samples were obtained from the shoulder muscle of healthy and myalgic subjects that performed a work and stress test. Samples from the baseline period and from the recovery period were analysed using gas chromatography—mass spectrometry (GC–MS) together with multivariate analysis to detect differences in extracellular content of metabolites between groups. Systematic differences in metabolites between groups were identified using multivariate analysis and orthogonal partial least square discriminate analysis (OPLS-DA). A complementary Mann–Whitney U test of group difference in individual metabolites was also performed. Results A large number of metabolites were detected and identified in this screening study. At baseline, no systematic differences between groups were observed according to the OPLS-DA. However, two metabolites, l -leucine and pyroglutamic acid, were significantly more abundant in the myalgic muscle compared to the healthy muscle. In the recovery period, systematic difference in metabolites between the groups was observed according to the OPLS-DA. The groups differed in amino acids, fatty acids and carbohydrates. Myristic acid and putrescine were significantly more abundant and beta- d -glucopyranose was significantly less abundant in the myalgic muscle. Conclusion This study provides important information regarding the metabolite content, thereby presenting new clues regarding the pathophysiology of the myalgic muscle.
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subjectAdult ; anatomi ; Annan medicinsk grundvetenskap ; Basic Medicine ; Biomedical and Life Sciences ; Biomedicine ; Case-Control Studies ; Environmental ; Exercise ; Extracellular Fluid - metabolism ; Fatty Acids - metabolism ; Female ; Fysiologi ; GC-MS ; Human Anatomy ; Human Physiology ; Humans ; Leucine - metabolism ; Medical and Health Sciences ; MEDICIN ; Medicin och hälsovetenskap ; MEDICINE ; Medicinska och farmaceutiska grundvetenskaper ; Metabolites ; Metabolome ; Metabolomics ; Metabolomics; Trapezius myalgia; Microdialysis; Repetitive work; Recovery; GC-MS; Metabolites ; Microdialysis ; Muscle, Skeletal - metabolism ; Muscle, Skeletal - pathology ; musculoskeletal diseases ; Myalgia - metabolism ; Myristic Acid ; Occupational Health ; Occupational Medicine/Industrial Medicine ; Original ; Original Article ; Orthopedics ; Other Basic Medicine ; Physiology ; Physiology (medical) ; Public Health ; Putrescine - metabolism ; Pyrrolidonecarboxylic Acid - metabolism ; Recovery ; Repetitive work ; Sports Medicine ; Trapezius myalgia
ispartofEuropean journal of applied physiology, 2013, Vol.113 (12), p.2977-2989
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descriptionPurpose The mechanisms behind trapezius myalgia are unclear. Many hypotheses have been presented suggesting an altered metabolism in the muscle. Here, muscle microdialysate from healthy and myalgic muscle is analysed using metabolomics. Metabolomics analyse a vast number of metabolites, enabling a comprehensive explorative screening of the cellular processes in the muscle. Methods Microdialysate samples were obtained from the shoulder muscle of healthy and myalgic subjects that performed a work and stress test. Samples from the baseline period and from the recovery period were analysed using gas chromatography—mass spectrometry (GC–MS) together with multivariate analysis to detect differences in extracellular content of metabolites between groups. Systematic differences in metabolites between groups were identified using multivariate analysis and orthogonal partial least square discriminate analysis (OPLS-DA). A complementary Mann–Whitney U test of group difference in individual metabolites was also performed. Results A large number of metabolites were detected and identified in this screening study. At baseline, no systematic differences between groups were observed according to the OPLS-DA. However, two metabolites, l -leucine and pyroglutamic acid, were significantly more abundant in the myalgic muscle compared to the healthy muscle. In the recovery period, systematic difference in metabolites between the groups was observed according to the OPLS-DA. The groups differed in amino acids, fatty acids and carbohydrates. Myristic acid and putrescine were significantly more abundant and beta- d -glucopyranose was significantly less abundant in the myalgic muscle. Conclusion This study provides important information regarding the metabolite content, thereby presenting new clues regarding the pathophysiology of the myalgic muscle.
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44Recovery
45Repetitive work
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titleComparative metabolomics of muscle interstitium fluid in human trapezius myalgia: an in vivo microdialysis study
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9Extracellular Fluid - metabolism
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atitleComparative metabolomics of muscle interstitium fluid in human trapezius myalgia: an in vivo microdialysis study
jtitleEuropean journal of applied physiology
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date2013-09-28
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notesCommunicated by Peter Krustrup.
abstractPurpose The mechanisms behind trapezius myalgia are unclear. Many hypotheses have been presented suggesting an altered metabolism in the muscle. Here, muscle microdialysate from healthy and myalgic muscle is analysed using metabolomics. Metabolomics analyse a vast number of metabolites, enabling a comprehensive explorative screening of the cellular processes in the muscle. Methods Microdialysate samples were obtained from the shoulder muscle of healthy and myalgic subjects that performed a work and stress test. Samples from the baseline period and from the recovery period were analysed using gas chromatography—mass spectrometry (GC–MS) together with multivariate analysis to detect differences in extracellular content of metabolites between groups. Systematic differences in metabolites between groups were identified using multivariate analysis and orthogonal partial least square discriminate analysis (OPLS-DA). A complementary Mann–Whitney U test of group difference in individual metabolites was also performed. Results A large number of metabolites were detected and identified in this screening study. At baseline, no systematic differences between groups were observed according to the OPLS-DA. However, two metabolites, l -leucine and pyroglutamic acid, were significantly more abundant in the myalgic muscle compared to the healthy muscle. In the recovery period, systematic difference in metabolites between the groups was observed according to the OPLS-DA. The groups differed in amino acids, fatty acids and carbohydrates. Myristic acid and putrescine were significantly more abundant and beta- d -glucopyranose was significantly less abundant in the myalgic muscle. Conclusion This study provides important information regarding the metabolite content, thereby presenting new clues regarding the pathophysiology of the myalgic muscle.
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