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Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity

CRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key elements in the interference step of the immune pathway. A CRISPR array composed of a series of repeats interspaced by spacer sequences ac... Full description

Journal Title: FEMS microbiology reviews 2015, Vol.39 (3), p.428-441
Main Author: Charpentier, Emmanuelle
Other Authors: Richter, Hagen , Oost, van der, John , White, Malcolm F
Format: Electronic Article Electronic Article
Language: English
Subjects:
RNA
Quelle: Alma/SFX Local Collection
Publisher: England: Oxford University Press
ID: ISSN: 0168-6445
Zum Text:
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recordid: cdi_wageningen_narcis_oai_library_wur_nl_wurpubs_495874
title: Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity
format: Article
creator:
  • Charpentier, Emmanuelle
  • Richter, Hagen
  • Oost, van der, John
  • White, Malcolm F
subjects:
  • Adaptive immunity
  • Adaptive systems
  • Archaea
  • Archaea - genetics
  • Bacteria - genetics
  • Basic Medicine
  • Biochemistry or Biopharmacy
  • biokemi eller biofarmaci
  • Biosynthesis
  • Cas5d
  • Cas6
  • Cas9
  • Clustered Regularly Interspaced Short Palindromic Repeats - genetics
  • Comparative analysis
  • CRISPR
  • crRNA biogenesis
  • Gene sequencing
  • Genomes
  • Immune system
  • Interspersed Repetitive Sequences - genetics
  • Medical and Health Sciences
  • Medical Biotechnology
  • Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology)
  • Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
  • Medical Genetics
  • Medicin och hälsovetenskap
  • Medicinsk bioteknologi
  • Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi)
  • Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
  • Medicinsk genetik
  • Medicinska och farmaceutiska grundvetenskaper
  • Microbiology
  • mikrobiologi
  • Molecular Biology
  • molekylärbiologi
  • Pathways
  • Proteins
  • QR180 Immunology
  • Review
  • Review Article
  • Ribonuclease III
  • Ribonucleases - metabolism
  • Ribonucleic acid
  • RNA
  • RNA - biosynthesis
  • RNA - genetics
  • RNase III
  • TracrRNA
ispartof: FEMS microbiology reviews, 2015, Vol.39 (3), p.428-441
description: CRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key elements in the interference step of the immune pathway. A CRISPR array composed of a series of repeats interspaced by spacer sequences acquired from invading mobile genomes is transcribed as a precursor crRNA (pre-crRNA) molecule. This pre-crRNA undergoes one or two maturation steps to generate the mature crRNAs that guide CRISPR-associated (Cas) protein(s) to cognate invading genomes for their destruction. Different types of CRISPR-Cas systems have evolved distinct crRNA biogenesis pathways that implicate highly sophisticated processing mechanisms. In Types I and III CRISPR-Cas systems, a specific endoribonuclease of the Cas6 family, either standalone or in a complex with other Cas proteins, cleaves the pre-crRNA within the repeat regions. In Type II systems, the trans-acting small RNA (tracrRNA) base pairs with each repeat of the pre-crRNA to form a dual-RNA that is cleaved by the housekeeping RNase III in the presence of the protein Cas9. In this review, we present a detailed comparative analysis of pre-crRNA recognition and cleavage mechanisms involved in the biogenesis of guide crRNAs in the three CRISPR-Cas types.
language: eng
source: Alma/SFX Local Collection
identifier: ISSN: 0168-6445
fulltext: fulltext
issn:
  • 0168-6445
  • 1574-6976
  • 1574-6976
url: Link


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descriptionCRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key elements in the interference step of the immune pathway. A CRISPR array composed of a series of repeats interspaced by spacer sequences acquired from invading mobile genomes is transcribed as a precursor crRNA (pre-crRNA) molecule. This pre-crRNA undergoes one or two maturation steps to generate the mature crRNAs that guide CRISPR-associated (Cas) protein(s) to cognate invading genomes for their destruction. Different types of CRISPR-Cas systems have evolved distinct crRNA biogenesis pathways that implicate highly sophisticated processing mechanisms. In Types I and III CRISPR-Cas systems, a specific endoribonuclease of the Cas6 family, either standalone or in a complex with other Cas proteins, cleaves the pre-crRNA within the repeat regions. In Type II systems, the trans-acting small RNA (tracrRNA) base pairs with each repeat of the pre-crRNA to form a dual-RNA that is cleaved by the housekeeping RNase III in the presence of the protein Cas9. In this review, we present a detailed comparative analysis of pre-crRNA recognition and cleavage mechanisms involved in the biogenesis of guide crRNAs in the three CRISPR-Cas types.
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subjectAdaptive immunity ; Adaptive systems ; Archaea ; Archaea - genetics ; Bacteria - genetics ; Basic Medicine ; Biochemistry or Biopharmacy ; biokemi eller biofarmaci ; Biosynthesis ; Cas5d ; Cas6 ; Cas9 ; Clustered Regularly Interspaced Short Palindromic Repeats - genetics ; Comparative analysis ; CRISPR ; crRNA biogenesis ; Gene sequencing ; Genomes ; Immune system ; Interspersed Repetitive Sequences - genetics ; Medical and Health Sciences ; Medical Biotechnology ; Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology) ; Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy) ; Medical Genetics ; Medicin och hälsovetenskap ; Medicinsk bioteknologi ; Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi) ; Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci) ; Medicinsk genetik ; Medicinska och farmaceutiska grundvetenskaper ; Microbiology ; mikrobiologi ; Molecular Biology ; molekylärbiologi ; Pathways ; Proteins ; QR180 Immunology ; Review ; Review Article ; Ribonuclease III ; Ribonucleases - metabolism ; Ribonucleic acid ; RNA ; RNA - biosynthesis ; RNA - genetics ; RNase III ; TracrRNA
ispartofFEMS microbiology reviews, 2015, Vol.39 (3), p.428-441
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descriptionCRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key elements in the interference step of the immune pathway. A CRISPR array composed of a series of repeats interspaced by spacer sequences acquired from invading mobile genomes is transcribed as a precursor crRNA (pre-crRNA) molecule. This pre-crRNA undergoes one or two maturation steps to generate the mature crRNAs that guide CRISPR-associated (Cas) protein(s) to cognate invading genomes for their destruction. Different types of CRISPR-Cas systems have evolved distinct crRNA biogenesis pathways that implicate highly sophisticated processing mechanisms. In Types I and III CRISPR-Cas systems, a specific endoribonuclease of the Cas6 family, either standalone or in a complex with other Cas proteins, cleaves the pre-crRNA within the repeat regions. In Type II systems, the trans-acting small RNA (tracrRNA) base pairs with each repeat of the pre-crRNA to form a dual-RNA that is cleaved by the housekeeping RNase III in the presence of the protein Cas9. In this review, we present a detailed comparative analysis of pre-crRNA recognition and cleavage mechanisms involved in the biogenesis of guide crRNAs in the three CRISPR-Cas types.
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0Adaptive immunity
1Adaptive systems
2Archaea
3Archaea - genetics
4Bacteria - genetics
5Basic Medicine
6Biochemistry or Biopharmacy
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8Biosynthesis
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16Gene sequencing
17Genomes
18Immune system
19Interspersed Repetitive Sequences - genetics
20Medical and Health Sciences
21Medical Biotechnology
22Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology)
23Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
24Medical Genetics
25Medicin och hälsovetenskap
26Medicinsk bioteknologi
27Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi)
28Medicinsk bioteknologi (med inriktning mot cellbiologi (inklusive stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci)
29Medicinsk genetik
30Medicinska och farmaceutiska grundvetenskaper
31Microbiology
32mikrobiologi
33Molecular Biology
34molekylärbiologi
35Pathways
36Proteins
37QR180 Immunology
38Review
39Review Article
40Ribonuclease III
41Ribonucleases - metabolism
42Ribonucleic acid
43RNA
44RNA - biosynthesis
45RNA - genetics
46RNase III
47TracrRNA
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abstractCRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key elements in the interference step of the immune pathway. A CRISPR array composed of a series of repeats interspaced by spacer sequences acquired from invading mobile genomes is transcribed as a precursor crRNA (pre-crRNA) molecule. This pre-crRNA undergoes one or two maturation steps to generate the mature crRNAs that guide CRISPR-associated (Cas) protein(s) to cognate invading genomes for their destruction. Different types of CRISPR-Cas systems have evolved distinct crRNA biogenesis pathways that implicate highly sophisticated processing mechanisms. In Types I and III CRISPR-Cas systems, a specific endoribonuclease of the Cas6 family, either standalone or in a complex with other Cas proteins, cleaves the pre-crRNA within the repeat regions. In Type II systems, the trans-acting small RNA (tracrRNA) base pairs with each repeat of the pre-crRNA to form a dual-RNA that is cleaved by the housekeeping RNase III in the presence of the protein Cas9. In this review, we present a detailed comparative analysis of pre-crRNA recognition and cleavage mechanisms involved in the biogenesis of guide crRNAs in the three CRISPR-Cas types.
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pmid25994611
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