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Oxidative phosphorylated neurofilament protein M protects spinal cord against ischemia/reperfusion injury - Oxidative phosphorylated neurofilament protein M protects spinal cord against ischemia/reperfusion injury

Previous studies have shown that neurofilament protein M expression is upregulated in the early stage of spinal cord ischemia/reperfusion injury, indicating that this protein may play a role in the injury process. In the present study, we compared protein expression in spinal cord tissue of rabbits... Full description

Journal Title: 中国神经再生研究:英文版 - Neural Regeneration Research 2014, Vol.9(18), pp.1672-1677
Main Author: Haitao
Other Authors: Wang , Su , Pan , Xiaoyu , Yang , Benqing , Zhu , Dalin , Wang
Format: Electronic Article Electronic Article
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ID: ISSN: 1673-5374
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title: Oxidative phosphorylated neurofilament protein M protects spinal cord against ischemia/reperfusion injury - Oxidative phosphorylated neurofilament protein M protects spinal cord against ischemia/reperfusion injury
format: Article
creator:
  • Haitao
  • Wang
  • Su
  • Pan
  • Xiaoyu
  • Yang
  • Benqing
  • Zhu
  • Dalin
  • Wang
subjects:
  • 缺血/再灌注损伤
  • 神经丝蛋白
  • 氧化磷酸化
  • M蛋白
  • 脊髓
  • 二维凝胶电泳
  • Blot分析
  • 蛋白表达
  • Nerve Regeneration
  • Neurofilament Protein M
  • Spinal Cord Injury
  • Ischemia/Reperfusion
  • Proteomics
  • Phosphorylation
  • Neuroprotection
  • Nsfc Grant
  • Neural Regeneration
ispartof: 中国神经再生研究:英文版 - Neural Regeneration Research, 2014, Vol.9(18), pp.1672-1677
description: Previous studies have shown that neurofilament protein M expression is upregulated in the early stage of spinal cord ischemia/reperfusion injury, indicating that this protein may play a role in the injury process. In the present study, we compared protein expression in spinal cord tissue of rabbits after 25 minutes of ischemia followed by 0, 12, 24, or 48 hours of reperfusion with that of sham operated rabbits, using proteomic two-dimensional gel electrophoresis and mass spec- trometry. In addition, the nerve repair-related neurofilament protein M with the unregulated expression was detected with immunohistochemistry and western blot analysis. Two-dimen- sional gel electrophoresis and mass spectrometry showed that, compared with the sham group, upregulation of protein expression was most significant in the spinal cords of rabbits that had undergone ischemia and 24 hours of reperfusion. Immunohistochemical analysis revealed that neurofilament protein M was located in the membrane and cytoplasm of neuronal soma and axons at each time point after injury. Western blot analysis showed that neurofilament protein M expression increased with reperfusion time until it peaked at 24 hours and returned to baseline level after 48 hours. Furthermore, neurofilament protein M is phosphorylated under oxidative stress, and expression changes were parallel for the phosphorylated and non-phosphorylated forms. Neurofilament protein M plays an important role in spinal cord ischemia/reperfusion injury, and its functions are achieved through oxidative phosphorylation.
language:
source:
identifier: ISSN: 1673-5374
fulltext: fulltext
issn:
  • 1673-5374
  • 16735374
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titleOxidative phosphorylated neurofilament protein M protects spinal cord against ischemia/reperfusion injury - Oxidative phosphorylated neurofilament protein M protects spinal cord against ischemia/reperfusion injury
creatorHaitao ; Wang ; Su ; Pan ; Xiaoyu ; Yang ; Benqing ; Zhu ; Dalin ; Wang
ispartof中国神经再生研究:英文版 - Neural Regeneration Research, 2014, Vol.9(18), pp.1672-1677
identifierISSN: 1673-5374
subject缺血/再灌注损伤 ; 神经丝蛋白 ; 氧化磷酸化 ; M蛋白 ; 脊髓 ; 二维凝胶电泳 ; Blot分析 ; 蛋白表达 ; Nerve Regeneration; Neurofilament Protein M; Spinal Cord Injury; Ischemia/Reperfusion;Proteomics; Phosphorylation; Neuroprotection; Nsfc Grant; Neural Regeneration
descriptionPrevious studies have shown that neurofilament protein M expression is upregulated in the early stage of spinal cord ischemia/reperfusion injury, indicating that this protein may play a role in the injury process. In the present study, we compared protein expression in spinal cord tissue of rabbits after 25 minutes of ischemia followed by 0, 12, 24, or 48 hours of reperfusion with that of sham operated rabbits, using proteomic two-dimensional gel electrophoresis and mass spec- trometry. In addition, the nerve repair-related neurofilament protein M with the unregulated expression was detected with immunohistochemistry and western blot analysis. Two-dimen- sional gel electrophoresis and mass spectrometry showed that, compared with the sham group, upregulation of protein expression was most significant in the spinal cords of rabbits that had undergone ischemia and 24 hours of reperfusion. Immunohistochemical analysis revealed that neurofilament protein M was located in the membrane and cytoplasm of neuronal soma and axons at each time point after injury. Western blot analysis showed that neurofilament protein M expression increased with reperfusion time until it peaked at 24 hours and returned to baseline level after 48 hours. Furthermore, neurofilament protein M is phosphorylated under oxidative stress, and expression changes were parallel for the phosphorylated and non-phosphorylated forms. Neurofilament protein M plays an important role in spinal cord ischemia/reperfusion injury, and its functions are achieved through oxidative phosphorylation.
relation作者单位: Department of Orthopedic Surgery, Affiliated Hospital of Beihua University, ]ilin, lilin Province, China Department of Orthopedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China Department of Orthopedics, People's Hospital of Tianjin, Tianjin, China
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titleOxidative phosphorylated neurofilament protein M protects spinal cord against ischemia/reperfusion injury
descriptionPrevious studies have shown that neurofilament protein M expression is upregulated in the early stage of spinal cord ischemia/reperfusion injury, indicating that this protein may play a role in the injury process. In the present study, we compared protein expression in spinal cord tissue of rabbits after 25 minutes of ischemia followed by 0, 12, 24, or 48 hours of reperfusion with that of sham operated rabbits, using proteomic two-dimensional gel electrophoresis and mass spec- trometry. In addition, the nerve repair-related neurofilament protein M with the unregulated expression was detected with immunohistochemistry and western blot analysis. Two-dimen- sional gel electrophoresis and mass spectrometry showed that, compared with the sham group, upregulation of protein expression was most significant in the spinal cords of rabbits that had undergone ischemia and 24 hours of reperfusion. Immunohistochemical analysis revealed that neurofilament protein M was located in the membrane and cytoplasm of neuronal soma and axons at each time point after injury. Western blot analysis showed that neurofilament protein M expression increased with reperfusion time until it peaked at 24 hours and returned to baseline level after 48 hours. Furthermore, neurofilament protein M is phosphorylated under oxidative stress, and expression changes were parallel for the phosphorylated and non-phosphorylated forms. Neurofilament protein M plays an important role in spinal cord ischemia/reperfusion injury, and its functions are achieved through oxidative phosphorylation.
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abstractPrevious studies have shown that neurofilament protein M expression is upregulated in the early stage of spinal cord ischemia/reperfusion injury, indicating that this protein may play a role in the injury process. In the present study, we compared protein expression in spinal cord tissue of rabbits after 25 minutes of ischemia followed by 0, 12, 24, or 48 hours of reperfusion with that of sham operated rabbits, using proteomic two-dimensional gel electrophoresis and mass spec- trometry. In addition, the nerve repair-related neurofilament protein M with the unregulated expression was detected with immunohistochemistry and western blot analysis. Two-dimen- sional gel electrophoresis and mass spectrometry showed that, compared with the sham group, upregulation of protein expression was most significant in the spinal cords of rabbits that had undergone ischemia and 24 hours of reperfusion. Immunohistochemical analysis revealed that neurofilament protein M was located in the membrane and cytoplasm of neuronal soma and axons at each time point after injury. Western blot analysis showed that neurofilament protein M expression increased with reperfusion time until it peaked at 24 hours and returned to baseline level after 48 hours. Furthermore, neurofilament protein M is phosphorylated under oxidative stress, and expression changes were parallel for the phosphorylated and non-phosphorylated forms. Neurofilament protein M plays an important role in spinal cord ischemia/reperfusion injury, and its functions are achieved through oxidative phosphorylation.
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