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中华按蚊GSTO1基因的鉴定及生物信息学分析 - Identification and Bioinformatics Analysis of the GST01 Gene in Anopheles sinensis

谷胱甘肽转移酶(GSTs)是有机体内一类具有解毒和抗氧化等多功能的超家族蛋白酶。昆虫中,谷胱甘肽转移酶主要分为七大家族,Omega 家族(GSTO)为研究较少的家族。在本研究中,通过 Blast 方法搜索中华按蚊(Anophelessinensis)转录组数据,得到中华按蚊 GSTO1 cDNA 序列,全长 1059 bp,其中阅读框 ORF 长 744 bp,编码 248 个氨基酸。推测该基因编码蛋白的分子量和等电点分别为 28.5 kDa 和 6.92。分析发现该蛋白不存在跨膜区和信号肽,亚细胞定位预测显示该蛋白质位于细胞质中。同源性分析表明 GSTO1 蛋白与达氏按蚊氨基酸序列相似性最高... Full description

Journal Title: 重庆师范大学学报:自然科学版 - Journal of Chongqing Normal University:Natural Science Edition 2015, Vol.32(03), pp.42-47
Main Author: 洪瑞
Other Authors: 李可人 , 吴传剑 , 闫振天 , 陈斌 , HONG Rui, LI Keren, WU Chuanjian, YAN Zhentian, CHEN Bin
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title: 中华按蚊GSTO1基因的鉴定及生物信息学分析 - Identification and Bioinformatics Analysis of the GST01 Gene in Anopheles sinensis
format: Article
creator:
  • 洪瑞
  • 李可人
  • 吴传剑
  • 闫振天
  • 陈斌
  • HONG Rui, LI Keren, WU Chuanjian, YAN Zhentian, CHEN Bin
subjects:
  • 谷胱甘肽
  • S
  • 转移酶
  • 中华按蚊(Anopheles
  • Sinensis)
  • Gsto1
  • 基因
  • 鉴定
  • 生物信息学分析
  • Glutathione S-Transferase
  • Anopheles Sinensis
  • Gsto1
  • Identification
  • Bioinformatic Analysis
ispartof: 重庆师范大学学报:自然科学版 - Journal of Chongqing Normal University:Natural Science Edition, 2015, Vol.32(03), pp.42-47
description: 谷胱甘肽转移酶(GSTs)是有机体内一类具有解毒和抗氧化等多功能的超家族蛋白酶。昆虫中,谷胱甘肽转移酶主要分为七大家族,Omega 家族(GSTO)为研究较少的家族。在本研究中,通过 Blast 方法搜索中华按蚊(Anophelessinensis)转录组数据,得到中华按蚊 GSTO1 cDNA 序列,全长 1059 bp,其中阅读框 ORF 长 744 bp,编码 248 个氨基酸。推测该基因编码蛋白的分子量和等电点分别为 28.5 kDa 和 6.92。分析发现该蛋白不存在跨膜区和信号肽,亚细胞定位预测显示该蛋白质位于细胞质中。同源性分析表明 GSTO1 蛋白与达氏按蚊氨基酸序列相似性最高。本研究进一步丰富了 GST 蛋白的基础数据,为进一步研究昆虫 GSTO 蛋白的功能奠定了理论基础。
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source: 维普数据 (Chongqing VIP Information Co.)
identifier: ISSN: 1672-6693
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issn:
  • 1672-6693
  • 16726693
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title中华按蚊GSTO1基因的鉴定及生物信息学分析 - Identification and Bioinformatics Analysis of the GST01 Gene in Anopheles sinensis
creator洪瑞 ; 李可人 ; 吴传剑 ; 闫振天 ; 陈斌 ; HONG Rui, LI Keren, WU Chuanjian, YAN Zhentian, CHEN Bin
ispartof重庆师范大学学报:自然科学版 - Journal of Chongqing Normal University:Natural Science Edition, 2015, Vol.32(03), pp.42-47
identifierISSN: 1672-6693
subject谷胱甘肽 ; S ; 转移酶 ; 中华按蚊(Anopheles ; Sinensis) ; Gsto1 ; 基因 ; 鉴定 ; 生物信息学分析 ; Glutathione S-Transferase;Anopheles Sinensis ; Gsto1 ; Identification; Bioinformatic Analysis
description
0谷胱甘肽转移酶(GSTs)是有机体内一类具有解毒和抗氧化等多功能的超家族蛋白酶。昆虫中,谷胱甘肽转移酶主要分为七大家族,Omega 家族(GSTO)为研究较少的家族。在本研究中,通过 Blast 方法搜索中华按蚊(Anophelessinensis)转录组数据,得到中华按蚊 GSTO1 cDNA 序列,全长 1059 bp,其中阅读框 ORF 长 744 bp,编码 248 个氨基酸。推测该基因编码蛋白的分子量和等电点分别为 28.5 kDa 和 6.92。分析发现该蛋白不存在跨膜区和信号肽,亚细胞定位预测显示该蛋白质位于细胞质中。同源性分析表明 GSTO1 蛋白与达氏按蚊氨基酸序列相似性最高。本研究进一步丰富了 GST 蛋白的基础数据,为进一步研究昆虫 GSTO 蛋白的功能奠定了理论基础。
1Glutathione S-transferees (GSTs) are superfamily of multifunctional enzymes, some of which have been reported to be rel- ative to insecticide resistance. In insects, GSTs have been classified into seven families, including Delta, Eplison, Omega, Sigma, Theta, Zeta and Unclassified. The study identified genes in the family Omega, named as AsGSTO1, from transcriptome in Anopheles sinensis. The full-length cDNA of the gene was 1059 bp with an opening reading frame (ORF) of 744 bp encoding 247 amino acids. The calculated molecular weight of AsGST01 is 28.5 kDa and the estimated pI is 6.92. The characteristics, homology and phylogenetic relationships of the AsGSTO1 were carried out through bioinformatic analyses. The AsGSTO1 shares the highest sequence similarity (92%) with the homolog of Anopheles darling. This work provides a base information frame for further research of the AsGST01 gene.
relation作者单位: 重庆师范大学昆虫与分子生物学研究所重庆市动物生物学重点实验室,重庆401331
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6重庆师范大学昆虫与分子生物学研究所重庆市动物生物学重点实验室,重庆401331
title
0中华按蚊GSTO1基因的鉴定及生物信息学分析
1Identification and Bioinformatics Analysis of the GST01 Gene in Anopheles sinensis
description
0谷胱甘肽转移酶(GSTs)是有机体内一类具有解毒和抗氧化等多功能的超家族蛋白酶。昆虫中,谷胱甘肽转移酶主要分为七大家族,Omega 家族(GSTO)为研究较少的家族。在本研究中,通过 Blast 方法搜索中华按蚊(Anophelessinensis)转录组数据,得到中华按蚊 GSTO1 cDNA 序列,全长 1059 bp,其中阅读框 ORF 长 744 bp,编码 248 个氨基酸。推测该基因编码蛋白的分子量和等电点分别为 28.5 kDa 和 6.92。分析发现该蛋白不存在跨膜区和信号肽,亚细胞定位预测显示该蛋白质位于细胞质中。同源性分析表明 GSTO1 蛋白与达氏按蚊氨基酸序列相似性最高。本研究进一步丰富了 GST 蛋白的基础数据,为进一步研究昆虫 GSTO 蛋白的功能奠定了理论基础。
1Glutathione S-transferees (GSTs) are superfamily of multifunctional enzymes, some of which have been reported to be rel- ative to insecticide resistance. In insects, GSTs have been classified into seven families, including Delta, Eplison, Omega, Sigma, Theta, Zeta and Unclassified. The study identified genes in the family Omega, named as AsGSTO1, from transcriptome in Anopheles sinensis. The full-length cDNA of the gene was 1059 bp with an opening reading frame (ORF) of 744 bp encoding 247 amino acids. The calculated molecular weight of AsGST01 is 28.5 kDa and the estimated pI is 6.92. The characteristics, homology and phylogenetic relationships of the AsGSTO1 were carried out through bioinformatic analyses. The AsGSTO1 shares the highest sequence similarity (92%) with the homolog of Anopheles darling. This work provides a base information frame for further research of the AsGST01 gene.
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1S
2转移酶
3中华按蚊(Anopheles
4Sinensis)
5Gsto1
6基因
7鉴定
8生物信息学分析
9Glutathione S-Transferase
10Anopheles Sinensis
11Identification
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1维普数据 (Chongqing VIP Information Co.)
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0重庆师范大学昆虫与分子生物学研究所重庆市动物生物学重点实验室,重庆401331
1HONG Rui, LI Keren, WU Chuanjian, YAN Zhentian, CHEN Bin (Institute of Entomology and Molecular Biology, Chongqing Key Laboratory of Animal Biology, Chongqing Normal University, Chongqing 401331, China)
citationpf 42 pt 47 vol 32 issue 03
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title中华按蚊GSTO1基因的鉴定及生物信息学分析 - Identification and Bioinformatics Analysis of the GST01 Gene in Anopheles sinensis
author洪瑞 ; 李可人 ; 吴传剑 ; 闫振天 ; 陈斌 ; HONG Rui, LI Keren, WU Chuanjian, YAN Zhentian, CHEN Bin
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9Glutathione S-Transferase;Anopheles Sinensis
10Identification; Bioinformatic Analysis
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atitle中华按蚊GSTO1基因的鉴定及生物信息学分析 - Identification and Bioinformatics Analysis of the GST01 Gene in Anopheles sinensis
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0谷胱甘肽转移酶(GSTs)是有机体内一类具有解毒和抗氧化等多功能的超家族蛋白酶。昆虫中,谷胱甘肽转移酶主要分为七大家族,Omega 家族(GSTO)为研究较少的家族。在本研究中,通过 Blast 方法搜索中华按蚊(Anophelessinensis)转录组数据,得到中华按蚊 GSTO1 cDNA 序列,全长 1059 bp,其中阅读框 ORF 长 744 bp,编码 248 个氨基酸。推测该基因编码蛋白的分子量和等电点分别为 28.5 kDa 和 6.92。分析发现该蛋白不存在跨膜区和信号肽,亚细胞定位预测显示该蛋白质位于细胞质中。同源性分析表明 GSTO1 蛋白与达氏按蚊氨基酸序列相似性最高。本研究进一步丰富了 GST 蛋白的基础数据,为进一步研究昆虫 GSTO 蛋白的功能奠定了理论基础。
1Glutathione S-transferees (GSTs) are superfamily of multifunctional enzymes, some of which have been reported to be rel- ative to insecticide resistance. In insects, GSTs have been classified into seven families, including Delta, Eplison, Omega, Sigma, Theta, Zeta and Unclassified. The study identified genes in the family Omega, named as AsGSTO1, from transcriptome in Anopheles sinensis. The full-length cDNA of the gene was 1059 bp with an opening reading frame (ORF) of 744 bp encoding 247 amino acids. The calculated molecular weight of AsGST01 is 28.5 kDa and the estimated pI is 6.92. The characteristics, homology and phylogenetic relationships of the AsGSTO1 were carried out through bioinformatic analyses. The AsGSTO1 shares the highest sequence similarity (92%) with the homolog of Anopheles darling. This work provides a base information frame for further research of the AsGST01 gene.
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